Restriction map for
pF1KE0977
(Circular) (Six-base) MAPSORT of: pF1KE0977.seq Check: 9330 from: 1 to: 4826
pF1KE0977 4826 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1864 1864
Size: 4826
Acc65I G'GTAC_C
Cuts at: 1842 1842
Size: 4826
AclI AA'CG_TT
Cuts at: 4666 4666
Size: 4826
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 2651 3083 3830 4098 338
Size: 2313 432 747 268 1066
Fragments arranged by size:
2313 1066 747 432 268
AflII C'TTAA_G
Cuts at: 364 364
Size: 4826
AflIII A'CryG_T
Cuts at: 150 268 2178 3282 4259 150
Size: 118 1910 1104 977 717
Fragments arranged by size:
1910 1104 977 717 118
AgeI A'CCGG_T
Cuts at: 3214 3214
Size: 4826
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1810 1810
Size: 4826
AlwNI CAG_nnn'CTG
Cuts at: 2131 3698 2131
Size: 1567 3259
ApaI G_GGCC'C
Cuts at: 1412 1553 1412
Size: 141 4685
ApaLI G'TGCA_C
Cuts at: 3596 3596
Size: 4826
ApoI r'AATT_y
Cuts at: 126 900 1040 1830 4179 4435 126
Size: 774 140 790 2349 256 517
Fragments arranged by size:
2349 790 774 517 256 140
AseI AT'TA_AT
Cuts at: 20 20
Size: 4826
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4826
AvaI C'yCGr_G
Cuts at: 1846 1846
Size: 4826
BamHI G'GATC_C
Cuts at: 990 1851 990
Size: 861 3965
BanI G'GyrC_C
Cuts at: 777 1842 2256 2504 2539 777
Size: 1065 414 248 35 3064
Fragments arranged by size:
3064 1065 414 248 35
BanII G_rGCy'C
Cuts at: 243 803 1412 1445 1553 1840 2870 243
Size: 560 609 33 108 287 1030 2199
Fragments arranged by size:
2199 1030 609 560 287 108 33
BbeI G_GCGC'C
Cuts at: 2260 2508 2260
Size: 248 4578
BbsI GAAGACnn'nnnn_
Cuts at: 1033 1033
Size: 4826
BbvCI CC'TCA_GC
Cuts at: 598 598
Size: 4826
BciVI GTATCCnnnnn_n'
Cuts at: 2724 3485 4826 2724
Size: 761 1341 2724
Fragments arranged by size:
2724 1341 761
BclI T'GATC_A
Cuts at: 2346 2346
Size: 4826
BglI GCCn_nnn'nGGC
Cuts at: 1639 1639
Size: 4826
BglII A'GATC_T
Cuts at: 2341 4378 2341
Size: 2037 2789
BlpI GC'TnA_GC
Cuts at: 1938 1938
Size: 4826
Bme1580I G_kGCm'C
Cuts at: 1412 1553 2451 2544 3600 1412
Size: 141 898 93 1056 2638
Fragments arranged by size:
2638 1056 898 141 93
BmrI ACTGGGnnnn_n'
Cuts at: 2038 2119 2454 4598 2038
Size: 81 335 2144 2266
Fragments arranged by size:
2266 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3193 3193
Size: 4826
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1054 1645 1748 1054
Size: 591 103 4132
Fragments arranged by size:
4132 591 103
Bpu10I CC'TnA_GC
Cuts at: 598 598
Size: 4826
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1558 1613 2002 2881 3373 3671 1558
Size: 55 389 879 492 298 2713
Fragments arranged by size:
2713 879 492 389 298 55
BsaI GGTCTCn'nnnn_
Cuts at: 4530 4530
Size: 4826
BsaAI yAC'GTr
Cuts at: 151 1240 2179 151
Size: 1089 939 2798
Fragments arranged by size:
2798 1089 939
BsaBI GATnn'nnATC
Cuts at: 6 2340 4377 6
Size: 2334 2037 455
Fragments arranged by size:
2334 2037 455
BsaHI Gr'CG_yC
Cuts at: 2257 2505 3207 4703 2257
Size: 248 702 1496 2380
Fragments arranged by size:
2380 1496 702 248
BsaWI w'CCGG_w
Cuts at: 876 2015 2536 3214 3488 3635 4046 876
Size: 1139 521 678 274 147 411 1656
Fragments arranged by size:
1656 1139 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 203 1741 203
Size: 1538 3288
BseYI C'CCAG_C
Cuts at: 1363 2251 3009 3586 4082 1363
Size: 888 758 577 496 2107
Fragments arranged by size:
2107 888 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2368 2414 3622 104
Size: 2264 46 1208 1308
Fragments arranged by size:
2264 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 243 1320 1840 2618 2808 3600 243
Size: 1077 520 778 190 792 1469
Fragments arranged by size:
1469 1077 792 778 520 190
Bsp1286I G_dGCh'C
Cuts at: 243 803 1320 1412 1445 1553 1840 2451 2544
Size: 560 517 92 33 108 287 611 93
Cuts at: 2544 2618 2808 2870 3600 243
Size: 74 190 62 730 1469
Fragments arranged by size:
1469 730 611 560 517 287 190 108
93 92 74 62 33
BspEI T'CCGG_A
Cuts at: 876 4046 876
Size: 3170 1656
BspHI T'CATG_A
Cuts at: 419 4002 419
Size: 3583 1243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 551 1367 1876 2090 2392 2773 551
Size: 816 509 214 302 381 2604
Fragments arranged by size:
2604 816 509 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3118 3172 4656 4821 50
Size: 3068 54 1484 165 55
Fragments arranged by size:
3068 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2738 2738
Size: 4826
BsrFI r'CCGG_y
Cuts at: 1268 1463 2824 3005 3214 1268
Size: 195 1361 181 209 2880
Fragments arranged by size:
2880 1361 209 195 181
BssSI C'ACGA_G
Cuts at: 1274 3097 3455 1274
Size: 1823 358 2645
Fragments arranged by size:
2645 1823 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2203 2203
Size: 4826
BstBI TT'CG_AA
Cuts at: 3187 3187
Size: 4826
BstYI r'GATC_y
Cuts at: 1 716 990 1157 1592 1851 2318 2341 2676
Size: 715 274 167 435 259 467 23 335
Cuts at: 2676 2922 3934 4020 4032 4378 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 715 467 449 435 346 335 274
259 246 167 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 448 782 1782 448
Size: 334 1000 3492
Fragments arranged by size:
3492 1000 334
BtgI C'CryG_G
Cuts at: 107 752 107
Size: 645 4181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 840 2776 2957 3120 840
Size: 1936 181 163 2546
Fragments arranged by size:
2546 1936 181 163
BtsI GCAGTG_nn'
Cuts at: 596 692 2069 596
Size: 96 1377 3353
Fragments arranged by size:
3353 1377 96
DraI TTT'AAA
Cuts at: 1825 1825
Size: 4826
DraIII CAC_nnn'GTG
Cuts at: 936 936
Size: 4826
DrdI GACnn_nn'nnGTC
Cuts at: 2532 3390 2532
Size: 858 3968
EaeI y'GGCC_r
Cuts at: 223 749 1270 2411 2585 2976 3003 4348 223
Size: 526 521 1141 174 391 27 1345 701
Fragments arranged by size:
1345 1141 701 526 521 391 174 27
EagI C'GGCC_G
Cuts at: 2411 2411
Size: 4826
EarI CTCTTCn'nnn_
Cuts at: 161 210 1058 2849 3059 161
Size: 49 848 1791 210 1928
Fragments arranged by size:
1928 1791 848 210 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3034 3344 3490 4636 3034
Size: 310 146 1146 3224
Fragments arranged by size:
3224 1146 310 146
EcoICRI GAG'CTC
Cuts at: 241 1838 241
Size: 1597 3229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 1054 1645 1748 2651 3083 3830 4098 338
Size: 716 591 103 903 432 747 268 1066
Fragments arranged by size:
1066 903 747 716 591 432 268 103
EcoO109I rG'GnC_Cy
Cuts at: 633 1326 1408 1550 1688 1965 633
Size: 693 82 142 138 277 3494
Fragments arranged by size:
3494 693 277 142 138 82
EcoRI G'AATT_C
Cuts at: 126 1830 126
Size: 1704 3122
EcoRV GAT'ATC
Cuts at: 1218 1218
Size: 4826
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2845 2845
Size: 4826
FspI TGC'GCA
Cuts at: 2607 2607
Size: 4826
HaeII r_GCGC'y
Cuts at: 2260 2508 3530 4509 2260
Size: 248 1022 979 2577
Fragments arranged by size:
2577 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 625 657 811 843 1175 1207 2668 2698 2700
Size: 32 154 32 332 32 1461 30 2
Cuts at: 2700 2730 625
Size: 30 2721
Fragments arranged by size:
2721 1461 332 154 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1305 1865 1305
Size: 560 4266
KasI G'GCGC_C
Cuts at: 2256 2504 2256
Size: 248 4578
KpnI G_GTAC'C
Cuts at: 1846 1846
Size: 4826
MfeI C'AATT_G
Cuts at: 131 481 131
Size: 350 4476
MluI A'CGCG_T
Cuts at: 268 4259 268
Size: 3991 835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3497 3681 3497
Size: 184 4642
MscI TGG'CCA
Cuts at: 225 2587 225
Size: 2362 2464
MslI CAynn'nnrTG
Cuts at: 589 2815 2942 589
Size: 2226 127 2473
Fragments arranged by size:
2473 2226 127
MspA1I CmG'CkG
Cuts at: 1624 1937 2251 2611 3624 3869 1624
Size: 313 314 360 1013 245 2581
Fragments arranged by size:
2581 1013 360 314 313 245
NaeI GCC'GGC
Cuts at: 3007 3007
Size: 4826
NarI GG'CG_CC
Cuts at: 2257 2505 2257
Size: 248 4578
NcoI C'CATG_G
Cuts at: 107 107
Size: 4826
NgoMIV G'CCGG_C
Cuts at: 3005 3005
Size: 4826
NspI r_CATG'y
Cuts at: 402 490 1879 2379 3286 402
Size: 88 1389 500 907 1942
Fragments arranged by size:
1942 1389 907 500 88
PasI CC'CwG_GG
Cuts at: 1692 1803 1692
Size: 111 4715
PciI A'CATG_T
Cuts at: 3282 3282
Size: 4826
PflMI CCAn_nnn'nTGG
Cuts at: 933 1279 933
Size: 346 4480
PfoI T'CCnGG_A
Cuts at: 511 1661 511
Size: 1150 3676
PmeI GTTT'AAAC
Cuts at: 1825 1825
Size: 4826
PmlI CAC'GTG
Cuts at: 151 151
Size: 4826
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3965 3997 3965
Size: 32 4794
PpuMI rG'GwC_Cy
Cuts at: 633 1326 633
Size: 693 4133
PsiI TTA'TAA
Cuts at: 247 247
Size: 4826
PspOMI G'GGCC_C
Cuts at: 1408 1549 1408
Size: 141 4685
PstI C_TGCA'G
Cuts at: 687 1091 1873 2558 687
Size: 404 782 685 2955
Fragments arranged by size:
2955 782 685 404
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4826
PvuII CAG'CTG
Cuts at: 1624 2251 2611 1624
Size: 627 360 3839
Fragments arranged by size:
3839 627 360
RsrII CG'GwC_CG
Cuts at: 3021 3021
Size: 4826
SacI G_AGCT'C
Cuts at: 243 1840 243
Size: 1597 3229
SalI G'TCGA_C
Cuts at: 1863 1863
Size: 4826
SapI GCTCTTCn'nnn_
Cuts at: 1058 2849 3059 1058
Size: 1791 210 2825
Fragments arranged by size:
2825 1791 210
SbfI CC_TGCA'GG
Cuts at: 1873 1873
Size: 4826
ScaI AGT'ACT
Cuts at: 4343 4343
Size: 4826
SfcI C'TryA_G
Cuts at: 33 683 1054 1087 1869 2554 3547 3738 33
Size: 650 371 33 782 685 993 191 1121
Fragments arranged by size:
1121 993 782 685 650 371 191 33
SfoI GGC'GCC
Cuts at: 2258 2506 2258
Size: 248 4578
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4826
SgrAI Cr'CCGG_yG
Cuts at: 1463 1463
Size: 4826
SmaI CCC'GGG
Cuts at: 1848 1848
Size: 4826
SmlI C'TyrA_G
Cuts at: 364 1573 1628 1981 2896 3388 3650 364
Size: 1209 55 353 915 492 262 1540
Fragments arranged by size:
1540 1209 915 492 353 262 55
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4826
SphI G_CATG'C
Cuts at: 490 1879 2379 490
Size: 1389 500 2937
Fragments arranged by size:
2937 1389 500
StuI AGG'CCT
Cuts at: 1793 1793
Size: 4826
StyI C'CwwG_G
Cuts at: 107 553 1960 2314 107
Size: 446 1407 354 2619
Fragments arranged by size:
2619 1407 446 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 448 2409 2542 3001 3210 448
Size: 1961 133 459 209 2064
Fragments arranged by size:
2064 1961 459 209 133
TatI w'GTAC_w
Cuts at: 411 1577 4341 411
Size: 1166 2764 896
Fragments arranged by size:
2764 1166 896
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4343 4375 4343
Size: 32 4794
Tth111I GACn'n_nGTC
Cuts at: 2623 2623
Size: 4826
XbaI T'CTAG_A
Cuts at: 1857 1857
Size: 4826
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1107 1669 2321 1107
Size: 562 652 3612
Fragments arranged by size:
3612 652 562
XmaI C'CCGG_G
Cuts at: 1846 1846
Size: 4826
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI EcoNI FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PshAI PspXI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI