Restriction map for
pF1KE0971
(Circular) (Six-base) MAPSORT of: pF1KE0971.seq Check: 5318 from: 1 to: 4733
pF1KE0971 4733 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 260 260
Size: 4733
AccI GT'mk_AC
Cuts at: 1771 1771
Size: 4733
Acc65I G'GTAC_C
Cuts at: 1749 1749
Size: 4733
AclI AA'CG_TT
Cuts at: 4573 4573
Size: 4733
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1337 1642 2558 2990 3737 4005 1337
Size: 305 916 432 747 268 2065
Fragments arranged by size:
2065 916 747 432 305 268
AflII C'TTAA_G
Cuts at: 297 297
Size: 4733
AflIII A'CryG_T
Cuts at: 2085 3189 4166 2085
Size: 1104 977 2652
Fragments arranged by size:
2652 1104 977
AgeI A'CCGG_T
Cuts at: 3121 3121
Size: 4733
AleI CACnn'nnGTG
Cuts at: 1076 1076
Size: 4733
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 151 506 151
Size: 355 4378
AlwNI CAG_nnn'CTG
Cuts at: 2038 3605 2038
Size: 1567 3166
ApaLI G'TGCA_C
Cuts at: 3503 3503
Size: 4733
ApoI r'AATT_y
Cuts at: 311 356 373 577 1606 1704 1737 4086 4342
Size: 45 17 204 1029 98 33 2349 256
Cuts at: 4342 311
Size: 702
Fragments arranged by size:
2349 1029 702 256 204 98 45 33
17
AseI AT'TA_AT
Cuts at: 20 857 20
Size: 837 3896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4733
AvaI C'yCGr_G
Cuts at: 1753 1753
Size: 4733
BamHI G'GATC_C
Cuts at: 1147 1424 1758 1147
Size: 277 334 4122
Fragments arranged by size:
4122 334 277
BanI G'GyrC_C
Cuts at: 187 1749 2163 2411 2446 187
Size: 1562 414 248 35 2474
Fragments arranged by size:
2474 1562 414 248 35
BanII G_rGCy'C
Cuts at: 1747 2777 1747
Size: 1030 3703
BbeI G_GCGC'C
Cuts at: 191 2167 2415 191
Size: 1976 248 2509
Fragments arranged by size:
2509 1976 248
BbsI GAAGACnn'nnnn_
Cuts at: 556 1448 1648 556
Size: 892 200 3641
Fragments arranged by size:
3641 892 200
BciVI GTATCCnnnnn_n'
Cuts at: 2631 3392 4733 2631
Size: 761 1341 2631
Fragments arranged by size:
2631 1341 761
BclI T'GATC_A
Cuts at: 1329 1511 2253 1329
Size: 182 742 3809
Fragments arranged by size:
3809 742 182
BglII A'GATC_T
Cuts at: 2248 4285 2248
Size: 2037 2696
BlpI GC'TnA_GC
Cuts at: 1845 1845
Size: 4733
Bme1580I G_kGCm'C
Cuts at: 930 2358 2451 3507 930
Size: 1428 93 1056 2156
Fragments arranged by size:
2156 1428 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1945 2026 2361 4505 1945
Size: 81 335 2144 2173
Fragments arranged by size:
2173 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3100 3100
Size: 4733
Bpu10I CC'TnA_GC
Cuts at: 178 178
Size: 4733
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1193 1223 1909 2788 3280 3578 1193
Size: 30 686 879 492 298 2348
Fragments arranged by size:
2348 879 686 492 298 30
BsaI GGTCTCn'nnnn_
Cuts at: 4437 4437
Size: 4733
BsaAI yAC'GTr
Cuts at: 898 2086 898
Size: 1188 3545
BsaBI GATnn'nnATC
Cuts at: 6 1695 2247 4284 6
Size: 1689 552 2037 455
Fragments arranged by size:
2037 1689 552 455
BsaHI Gr'CG_yC
Cuts at: 188 2164 2412 3114 4610 188
Size: 1976 248 702 1496 311
Fragments arranged by size:
1976 1496 702 311 248
BsaWI w'CCGG_w
Cuts at: 1922 2443 3121 3395 3542 3953 1922
Size: 521 678 274 147 411 2702
Fragments arranged by size:
2702 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 515 1196 515
Size: 681 4052
BseYI C'CCAG_C
Cuts at: 1415 2158 2916 3493 3989 1415
Size: 743 758 577 496 2159
Fragments arranged by size:
2159 758 743 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 496 641 238
Size: 258 145 4330
Fragments arranged by size:
4330 258 145
BsiEI CG_ry'CG
Cuts at: 104 2275 2321 3529 104
Size: 2171 46 1208 1308
Fragments arranged by size:
2171 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 897 1747 2525 2715 3507 897
Size: 850 778 190 792 2123
Fragments arranged by size:
2123 850 792 778 190
BsmI GAATG_Cn'
Cuts at: 1321 1321
Size: 4733
Bsp1286I G_dGCh'C
Cuts at: 897 930 1747 2358 2451 2525 2715 2777 3507
Size: 33 817 611 93 74 190 62 730
Cuts at: 3507 897
Size: 2123
Fragments arranged by size:
2123 817 730 611 190 93 74 62
33
BspEI T'CCGG_A
Cuts at: 3953 3953
Size: 4733
BspHI T'CATG_A
Cuts at: 540 1514 3909 540
Size: 974 2395 1364
Fragments arranged by size:
2395 1364 974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 176 260 518 1783 1997 2299 2680 176
Size: 84 258 1265 214 302 381 2229
Fragments arranged by size:
2229 1265 381 302 258 214 84
BsrBI CCG'CTC
Cuts at: 50 3025 3079 4563 4728 50
Size: 2975 54 1484 165 55
Fragments arranged by size:
2975 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 369 2645 369
Size: 2276 2457
BsrFI r'CCGG_y
Cuts at: 2731 2912 3121 2731
Size: 181 209 4343
Fragments arranged by size:
4343 209 181
BssSI C'ACGA_G
Cuts at: 3004 3362 3004
Size: 358 4375
BstAPI GCAn_nnn'nTGC
Cuts at: 2110 2110
Size: 4733
BstBI TT'CG_AA
Cuts at: 3094 3094
Size: 4733
BstYI r'GATC_y
Cuts at: 1 1147 1306 1424 1758 2225 2248 2583 2829
Size: 1146 159 118 334 467 23 335 246
Cuts at: 2829 3841 3927 3939 4285 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1146 1012 467 449 346 335 334 246
159 118 86 23 12
BtgI C'CryG_G
Cuts at: 183 183
Size: 4733
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2683 2864 3027 2683
Size: 181 163 4389
Fragments arranged by size:
4389 181 163
BtsI GCAGTG_nn'
Cuts at: 1426 1976 1426
Size: 550 4183
DraI TTT'AAA
Cuts at: 1703 1732 1703
Size: 29 4704
DrdI GACnn_nn'nnGTC
Cuts at: 229 2439 3297 229
Size: 2210 858 1665
Fragments arranged by size:
2210 1665 858
EaeI y'GGCC_r
Cuts at: 239 460 2318 2492 2883 2910 4255 239
Size: 221 1858 174 391 27 1345 717
Fragments arranged by size:
1858 1345 717 391 221 174 27
EagI C'GGCC_G
Cuts at: 2318 2318
Size: 4733
EarI CTCTTCn'nnn_
Cuts at: 995 1356 2756 2966 995
Size: 361 1400 210 2762
Fragments arranged by size:
2762 1400 361 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 198 2941 3251 3397 4543 198
Size: 2743 310 146 1146 388
Fragments arranged by size:
2743 1146 388 310 146
EcoICRI GAG'CTC
Cuts at: 1745 1745
Size: 4733
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1337 1642 2558 2990 3737 4005 1337
Size: 305 916 432 747 268 2065
Fragments arranged by size:
2065 916 747 432 305 268
EcoO109I rG'GnC_Cy
Cuts at: 1193 1556 1872 1193
Size: 363 316 4054
Fragments arranged by size:
4054 363 316
EcoRI G'AATT_C
Cuts at: 1737 1737
Size: 4733
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 312 968 1489 2752 312
Size: 656 521 1263 2293
Fragments arranged by size:
2293 1263 656 521
FspI TGC'GCA
Cuts at: 2514 2514
Size: 4733
HaeII r_GCGC'y
Cuts at: 134 191 2167 2415 3437 4416 134
Size: 57 1976 248 1022 979 451
Fragments arranged by size:
1976 1022 979 451 248 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 461 493 1210 1242 2575 2605 2607 2637 461
Size: 32 717 32 1333 30 2 30 2557
Fragments arranged by size:
2557 1333 717 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1772 1772
Size: 4733
KasI G'GCGC_C
Cuts at: 187 2163 2411 187
Size: 1976 248 2509
Fragments arranged by size:
2509 1976 248
KpnI G_GTAC'C
Cuts at: 1753 1753
Size: 4733
MluI A'CGCG_T
Cuts at: 4166 4166
Size: 4733
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3404 3588 3404
Size: 184 4549
MscI TGG'CCA
Cuts at: 462 2494 462
Size: 2032 2701
MslI CAynn'nnrTG
Cuts at: 1076 2722 2849 1076
Size: 1646 127 2960
Fragments arranged by size:
2960 1646 127
MspA1I CmG'CkG
Cuts at: 185 270 1844 2158 2518 3531 3776 185
Size: 85 1574 314 360 1013 245 1142
Fragments arranged by size:
1574 1142 1013 360 314 245 85
NaeI GCC'GGC
Cuts at: 2914 2914
Size: 4733
NarI GG'CG_CC
Cuts at: 188 2164 2412 188
Size: 1976 248 2509
Fragments arranged by size:
2509 1976 248
NdeI CA'TA_TG
Cuts at: 876 876
Size: 4733
NgoMIV G'CCGG_C
Cuts at: 2912 2912
Size: 4733
NspI r_CATG'y
Cuts at: 402 1786 2286 3193 402
Size: 1384 500 907 1942
Fragments arranged by size:
1942 1384 907 500
PciI A'CATG_T
Cuts at: 3189 3189
Size: 4733
PfoI T'CCnGG_A
Cuts at: 1272 1272
Size: 4733
PmeI GTTT'AAAC
Cuts at: 1732 1732
Size: 4733
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3872 3904 3872
Size: 32 4701
PpuMI rG'GwC_Cy
Cuts at: 1193 1556 1193
Size: 363 4370
PstI C_TGCA'G
Cuts at: 130 1383 1403 1780 2465 130
Size: 1253 20 377 685 2398
Fragments arranged by size:
2398 1253 685 377 20
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4733
PvuII CAG'CTG
Cuts at: 2158 2518 2158
Size: 360 4373
RsrII CG'GwC_CG
Cuts at: 2928 2928
Size: 4733
SacI G_AGCT'C
Cuts at: 1747 1747
Size: 4733
SacII CC_GC'GG
Cuts at: 186 186
Size: 4733
SalI G'TCGA_C
Cuts at: 1770 1770
Size: 4733
SapI GCTCTTCn'nnn_
Cuts at: 1356 2756 2966 1356
Size: 1400 210 3123
Fragments arranged by size:
3123 1400 210
SbfI CC_TGCA'GG
Cuts at: 1780 1780
Size: 4733
ScaI AGT'ACT
Cuts at: 4250 4250
Size: 4733
SfcI C'TryA_G
Cuts at: 33 126 1379 1399 1776 2461 3454 3645 33
Size: 93 1253 20 377 685 993 191 1121
Fragments arranged by size:
1253 1121 993 685 377 191 93 20
SfoI GGC'GCC
Cuts at: 189 2165 2413 189
Size: 1976 248 2509
Fragments arranged by size:
2509 1976 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4733
SmaI CCC'GGG
Cuts at: 1755 1755
Size: 4733
SmlI C'TyrA_G
Cuts at: 297 1208 1238 1888 2803 3295 3557 297
Size: 911 30 650 915 492 262 1473
Fragments arranged by size:
1473 915 911 650 492 262 30
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4733
SphI G_CATG'C
Cuts at: 1786 2286 1786
Size: 500 4233
StyI C'CwwG_G
Cuts at: 495 1596 1867 2221 495
Size: 1101 271 354 3007
Fragments arranged by size:
3007 1101 354 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2316 2449 2908 3117 2316
Size: 133 459 209 3932
Fragments arranged by size:
3932 459 209 133
TatI w'GTAC_w
Cuts at: 1623 4248 1623
Size: 2625 2108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4250 4282 4250
Size: 32 4701
Tth111I GACn'n_nGTC
Cuts at: 2530 2530
Size: 4733
XbaI T'CTAG_A
Cuts at: 1228 1687 1764 1228
Size: 459 77 4197
Fragments arranged by size:
4197 459 77
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1168 2228 1168
Size: 1060 3673
XmaI C'CCGG_G
Cuts at: 1753 1753
Size: 4733
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AloI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI BsaXI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI