Restriction map for
pF1KE0964
(Circular) (Six-base) MAPSORT of: pF1KE0964.seq Check: 6582 from: 1 to: 4637
pF1KE0964 4637 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1675 1675
Size: 4637
Acc65I G'GTAC_C
Cuts at: 514 1653 514
Size: 1139 3498
AclI AA'CG_TT
Cuts at: 4477 4477
Size: 4637
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 2462 2894 3641 3909 889
Size: 1573 432 747 268 1617
Fragments arranged by size:
1617 1573 747 432 268
AfeI AGC'GCT
Cuts at: 1215 1215
Size: 4637
AflIII A'CryG_T
Cuts at: 279 1989 3093 4070 279
Size: 1710 1104 977 846
Fragments arranged by size:
1710 1104 977 846
AgeI A'CCGG_T
Cuts at: 3025 3025
Size: 4637
AlwNI CAG_nnn'CTG
Cuts at: 867 962 1942 3509 867
Size: 95 980 1567 1995
Fragments arranged by size:
1995 1567 980 95
ApaLI G'TGCA_C
Cuts at: 3407 3407
Size: 4637
ApoI r'AATT_y
Cuts at: 331 404 436 1040 1641 3990 4246 331
Size: 73 32 604 601 2349 256 722
Fragments arranged by size:
2349 722 604 601 256 73 32
AseI AT'TA_AT
Cuts at: 20 20
Size: 4637
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
AvaI C'yCGr_G
Cuts at: 1657 1657
Size: 4637
BamHI G'GATC_C
Cuts at: 1662 1662
Size: 4637
BanI G'GyrC_C
Cuts at: 514 1653 2067 2315 2350 514
Size: 1139 414 248 35 2801
Fragments arranged by size:
2801 1139 414 248 35
BanII G_rGCy'C
Cuts at: 920 945 1651 2681 920
Size: 25 706 1030 2876
Fragments arranged by size:
2876 1030 706 25
BbeI G_GCGC'C
Cuts at: 2071 2319 2071
Size: 248 4389
BbsI GAAGACnn'nnnn_
Cuts at: 1311 1311
Size: 4637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4603
BciVI GTATCCnnnnn_n'
Cuts at: 559 2535 3296 4637 559
Size: 1976 761 1341 559
Fragments arranged by size:
1976 1341 761 559
BclI T'GATC_A
Cuts at: 2157 2157
Size: 4637
BglII A'GATC_T
Cuts at: 497 1444 2152 4189 497
Size: 947 708 2037 945
Fragments arranged by size:
2037 947 945 708
BlpI GC'TnA_GC
Cuts at: 1749 1749
Size: 4637
Bme1580I G_kGCm'C
Cuts at: 2262 2355 3411 2262
Size: 93 1056 3488
Fragments arranged by size:
3488 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 208 918 1527 1849 1930 2265 4409 208
Size: 710 609 322 81 335 2144 436
Fragments arranged by size:
2144 710 609 436 335 322 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3004 3004
Size: 4637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 239
Size: 4637
Bpu10I CC'TnA_GC
Cuts at: 1286 1286
Size: 4637
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1310 1813 2692 3184 3482 1018
Size: 292 503 879 492 298 2173
Fragments arranged by size:
2173 879 503 492 298 292
BsaI GGTCTCn'nnnn_
Cuts at: 4341 4341
Size: 4637
BsaAI yAC'GTr
Cuts at: 1990 1990
Size: 4637
BsaBI GATnn'nnATC
Cuts at: 6 1449 2151 4188 6
Size: 1443 702 2037 455
Fragments arranged by size:
2037 1443 702 455
BsaHI Gr'CG_yC
Cuts at: 2068 2316 3018 4514 2068
Size: 248 702 1496 2191
Fragments arranged by size:
2191 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1826 2347 3025 3299 3446 3857 1826
Size: 521 678 274 147 411 2606
Fragments arranged by size:
2606 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1264 1264
Size: 4637
BseYI C'CCAG_C
Cuts at: 2062 2820 3397 3893 2062
Size: 758 577 496 2806
Fragments arranged by size:
2806 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1261 907
Size: 354 4283
BsiEI CG_ry'CG
Cuts at: 104 2179 2225 3433 104
Size: 2075 46 1208 1308
Fragments arranged by size:
2075 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 920 993 1651 2429 2619 3411 920
Size: 73 658 778 190 792 2146
Fragments arranged by size:
2146 792 778 658 190 73
Bsp1286I G_dGCh'C
Cuts at: 920 945 993 1651 2262 2355 2429 2619 2681
Size: 25 48 658 611 93 74 190 62
Cuts at: 2681 3411 920
Size: 730 2146
Fragments arranged by size:
2146 730 658 611 190 93 74 62
48 25
BspEI T'CCGG_A
Cuts at: 3857 3857
Size: 4637
BspHI T'CATG_A
Cuts at: 3813 3813
Size: 4637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1687 1901 2203 2584 1687
Size: 214 302 381 3740
Fragments arranged by size:
3740 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2929 2983 4467 4632 50
Size: 2879 54 1484 165 55
Fragments arranged by size:
2879 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2549 2549
Size: 4637
BsrFI r'CCGG_y
Cuts at: 2635 2816 3025 2635
Size: 181 209 4247
Fragments arranged by size:
4247 209 181
BssSI C'ACGA_G
Cuts at: 2908 3266 2908
Size: 358 4279
BstAPI GCAn_nnn'nTGC
Cuts at: 2014 2014
Size: 4637
BstBI TT'CG_AA
Cuts at: 2998 2998
Size: 4637
BstYI r'GATC_y
Cuts at: 1 421 497 809 1444 1662 2129 2152 2487
Size: 420 76 312 635 218 467 23 335
Cuts at: 2487 2733 3745 3831 3843 4189 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 635 467 449 420 346 335 312
246 218 86 76 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2587 2768 2931 2587
Size: 181 163 4293
Fragments arranged by size:
4293 181 163
BtsI GCAGTG_nn'
Cuts at: 1880 1880
Size: 4637
DraI TTT'AAA
Cuts at: 522 1636 522
Size: 1114 3523
DrdI GACnn_nn'nnGTC
Cuts at: 2343 3201 2343
Size: 858 3779
EaeI y'GGCC_r
Cuts at: 2222 2396 2787 2814 4159 2222
Size: 174 391 27 1345 2700
Fragments arranged by size:
2700 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2222 2222
Size: 4637
EarI CTCTTCn'nnn_
Cuts at: 1544 2660 2870 1544
Size: 1116 210 3311
Fragments arranged by size:
3311 1116 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2845 3155 3301 4447 2845
Size: 310 146 1146 3035
Fragments arranged by size:
3035 1146 310 146
EcoICRI GAG'CTC
Cuts at: 918 1649 918
Size: 731 3906
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 889 2462 2894 3641 3909 239
Size: 650 1573 432 747 268 967
Fragments arranged by size:
1573 967 747 650 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 728 728
Size: 4637
EcoO109I rG'GnC_Cy
Cuts at: 873 1776 873
Size: 903 3734
EcoRI G'AATT_C
Cuts at: 404 436 1641 404
Size: 32 1205 3400
Fragments arranged by size:
3400 1205 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 4637
FspI TGC'GCA
Cuts at: 2418 2418
Size: 4637
HaeII r_GCGC'y
Cuts at: 1217 2071 2319 3341 4320 1217
Size: 854 248 1022 979 1534
Fragments arranged by size:
1534 1022 979 854 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2479 2509 2511 2541 2479
Size: 30 2 30 4575
Fragments arranged by size:
4575 30 30 2
HincII GTy'rAC
Cuts at: 530 1164 1676 530
Size: 634 512 3491
Fragments arranged by size:
3491 634 512
KasI G'GCGC_C
Cuts at: 2067 2315 2067
Size: 248 4389
KpnI G_GTAC'C
Cuts at: 518 1657 518
Size: 1139 3498
MluI A'CGCG_T
Cuts at: 4070 4070
Size: 4637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 539 890 3308 3492 539
Size: 351 2418 184 1684
Fragments arranged by size:
2418 1684 351 184
MscI TGG'CCA
Cuts at: 2398 2398
Size: 4637
MslI CAynn'nnrTG
Cuts at: 584 602 2626 2753 584
Size: 18 2024 127 2468
Fragments arranged by size:
2468 2024 127 18
MspA1I CmG'CkG
Cuts at: 1748 2062 2422 3435 3680 1748
Size: 314 360 1013 245 2705
Fragments arranged by size:
2705 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2818 2818
Size: 4637
NarI GG'CG_CC
Cuts at: 2068 2316 2068
Size: 248 4389
NdeI CA'TA_TG
Cuts at: 1005 1005
Size: 4637
NgoMIV G'CCGG_C
Cuts at: 2816 2816
Size: 4637
NspI r_CATG'y
Cuts at: 283 828 1690 2190 3097 283
Size: 545 862 500 907 1823
Fragments arranged by size:
1823 907 862 545 500
PasI CC'CwG_GG
Cuts at: 854 854
Size: 4637
PciI A'CATG_T
Cuts at: 279 3093 279
Size: 2814 1823
PflMI CCAn_nnn'nTGG
Cuts at: 981 981
Size: 4637
PmeI GTTT'AAAC
Cuts at: 1636 1636
Size: 4637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 4605
PpuMI rG'GwC_Cy
Cuts at: 873 873
Size: 4637
PstI C_TGCA'G
Cuts at: 1684 2369 1684
Size: 685 3952
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4637
PvuII CAG'CTG
Cuts at: 2062 2422 2062
Size: 360 4277
RsrII CG'GwC_CG
Cuts at: 2832 2832
Size: 4637
SacI G_AGCT'C
Cuts at: 920 1651 920
Size: 731 3906
SalI G'TCGA_C
Cuts at: 1674 1674
Size: 4637
SapI GCTCTTCn'nnn_
Cuts at: 2660 2870 2660
Size: 210 4427
SbfI CC_TGCA'GG
Cuts at: 1684 1684
Size: 4637
ScaI AGT'ACT
Cuts at: 4154 4154
Size: 4637
SexAI A'CCwGG_T
Cuts at: 647 647
Size: 4637
SfcI C'TryA_G
Cuts at: 33 678 1680 2365 3358 3549 33
Size: 645 1002 685 993 191 1121
Fragments arranged by size:
1121 1002 993 685 645 191
SfoI GGC'GCC
Cuts at: 2069 2317 2069
Size: 248 4389
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
SmaI CCC'GGG
Cuts at: 1659 1659
Size: 4637
SmlI C'TyrA_G
Cuts at: 1033 1325 1792 2707 3199 3461 1033
Size: 292 467 915 492 262 2209
Fragments arranged by size:
2209 915 492 467 292 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4637
SphI G_CATG'C
Cuts at: 828 1690 2190 828
Size: 862 500 3275
Fragments arranged by size:
3275 862 500
StyI C'CwwG_G
Cuts at: 1771 2125 1771
Size: 354 4283
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2220 2353 2812 3021 2220
Size: 133 459 209 3836
Fragments arranged by size:
3836 459 209 133
TatI w'GTAC_w
Cuts at: 564 4152 564
Size: 3588 1049
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4154 4186 4154
Size: 32 4605
Tth111I GACn'n_nGTC
Cuts at: 2434 2434
Size: 4637
XbaI T'CTAG_A
Cuts at: 1668 1668
Size: 4637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2132 2132
Size: 4637
XmaI C'CCGG_G
Cuts at: 1657 1657
Size: 4637
XmnI GAAnn'nnTTC
Cuts at: 593 593
Size: 4637
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NheI NotI NruI
NsiI PacI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI