Restriction map for
pF1KE0963
(Circular) (Six-base) MAPSORT of: pF1KE0963.seq Check: 9606 from: 1 to: 4580
pF1KE0963 4580 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1618 1618
Size: 4580
Acc65I G'GTAC_C
Cuts at: 1596 1596
Size: 4580
AclI AA'CG_TT
Cuts at: 4420 4420
Size: 4580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 428 781 1418 1558 2405 2837 3584 3852
Size: 87 353 637 140 847 432 747 268
Cuts at: 3852 341
Size: 1069
Fragments arranged by size:
1069 847 747 637 432 353 268 140
87
AflII C'TTAA_G
Cuts at: 700 1312 700
Size: 612 3968
AflIII A'CryG_T
Cuts at: 471 1932 3036 4013 471
Size: 1461 1104 977 1038
Fragments arranged by size:
1461 1104 1038 977
AgeI A'CCGG_T
Cuts at: 2968 2968
Size: 4580
AhdI GACnn_n'nnGTC
Cuts at: 663 1527 663
Size: 864 3716
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 785 817 1101 1133 785
Size: 32 284 32 4232
Fragments arranged by size:
4232 284 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1885 3452 1885
Size: 1567 3013
ApaLI G'TGCA_C
Cuts at: 3350 3350
Size: 4580
ApoI r'AATT_y
Cuts at: 243 623 954 1041 1584 3933 4189 243
Size: 380 331 87 543 2349 256 634
Fragments arranged by size:
2349 634 543 380 331 256 87
AseI AT'TA_AT
Cuts at: 20 1034 20
Size: 1014 3566
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4580
AvaI C'yCGr_G
Cuts at: 866 907 1600 866
Size: 41 693 3846
Fragments arranged by size:
3846 693 41
BamHI G'GATC_C
Cuts at: 1452 1605 1452
Size: 153 4427
BanI G'GyrC_C
Cuts at: 1596 2010 2258 2293 1596
Size: 414 248 35 3883
Fragments arranged by size:
3883 414 248 35
BanII G_rGCy'C
Cuts at: 148 162 1594 2624 148
Size: 14 1432 1030 2104
Fragments arranged by size:
2104 1432 1030 14
BbeI G_GCGC'C
Cuts at: 2014 2262 2014
Size: 248 4332
BbsI GAAGACnn'nnnn_
Cuts at: 416 550 416
Size: 134 4446
BciVI GTATCCnnnnn_n'
Cuts at: 2478 3239 4580 2478
Size: 761 1341 2478
Fragments arranged by size:
2478 1341 761
BclI T'GATC_A
Cuts at: 2100 2100
Size: 4580
BfrBI ATG'CAT
Cuts at: 1189 1189
Size: 4580
BglII A'GATC_T
Cuts at: 2095 4132 2095
Size: 2037 2543
BlpI GC'TnA_GC
Cuts at: 1692 1692
Size: 4580
Bme1580I G_kGCm'C
Cuts at: 2205 2298 3354 2205
Size: 93 1056 3431
Fragments arranged by size:
3431 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1533 1792 1873 2208 4352 1533
Size: 259 81 335 2144 1761
Fragments arranged by size:
2144 1761 335 259 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2947 2947
Size: 4580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 602 1188 602
Size: 586 3994
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1486 1756 2635 3127 3425 1486
Size: 270 879 492 298 2641
Fragments arranged by size:
2641 879 492 298 270
BsaI GGTCTCn'nnnn_
Cuts at: 1279 4284 1279
Size: 3005 1575
BsaAI yAC'GTr
Cuts at: 1933 1933
Size: 4580
BsaBI GATnn'nnATC
Cuts at: 6 2094 4131 6
Size: 2088 2037 455
Fragments arranged by size:
2088 2037 455
BsaHI Gr'CG_yC
Cuts at: 2011 2259 2961 4457 2011
Size: 248 702 1496 2134
Fragments arranged by size:
2134 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1769 2290 2968 3242 3389 3800 1769
Size: 521 678 274 147 411 2549
Fragments arranged by size:
2549 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1498 1516 1498
Size: 18 4562
BseYI C'CCAG_C
Cuts at: 2005 2763 3340 3836 2005
Size: 758 577 496 2749
Fragments arranged by size:
2749 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2122 2168 3376 104
Size: 2018 46 1208 1308
Fragments arranged by size:
2018 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 162 1594 2372 2562 3354 162
Size: 1432 778 190 792 1388
Fragments arranged by size:
1432 1388 792 778 190
BsmI GAATG_Cn'
Cuts at: 1323 1323
Size: 4580
BsmBI CGTCTCn'nnnn_
Cuts at: 782 782
Size: 4580
Bsp1286I G_dGCh'C
Cuts at: 148 162 1594 2205 2298 2372 2562 2624 3354
Size: 14 1432 611 93 74 190 62 730
Cuts at: 3354 148
Size: 1374
Fragments arranged by size:
1432 1374 730 611 190 93 74 62
14
BspEI T'CCGG_A
Cuts at: 3800 3800
Size: 4580
BspHI T'CATG_A
Cuts at: 3756 3756
Size: 4580
BspMI ACCTGCnnnn'nnnn_
Cuts at: 644 1630 1844 2146 2527 644
Size: 986 214 302 381 2697
Fragments arranged by size:
2697 986 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2872 2926 4410 4575 50
Size: 2822 54 1484 165 55
Fragments arranged by size:
2822 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2492 2492
Size: 4580
BsrFI r'CCGG_y
Cuts at: 2578 2759 2968 2578
Size: 181 209 4190
Fragments arranged by size:
4190 209 181
BssSI C'ACGA_G
Cuts at: 2851 3209 2851
Size: 358 4222
BstAPI GCAn_nnn'nTGC
Cuts at: 1957 1957
Size: 4580
BstBI TT'CG_AA
Cuts at: 958 2941 958
Size: 1983 2597
BstXI CCAn_nnnn'nTGG
Cuts at: 573 626 573
Size: 53 4527
BstYI r'GATC_y
Cuts at: 1 363 547 1452 1605 2072 2095 2430 2676
Size: 362 184 905 153 467 23 335 246
Cuts at: 2676 3688 3774 3786 4132 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 905 467 449 362 346 335 246
184 153 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4580
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 823 2530 2711 2874 823
Size: 1707 181 163 2529
Fragments arranged by size:
2529 1707 181 163
BtsI GCAGTG_nn'
Cuts at: 769 1279 1823 769
Size: 510 544 3526
Fragments arranged by size:
3526 544 510
DraI TTT'AAA
Cuts at: 822 1579 822
Size: 757 3823
DrdI GACnn_nn'nnGTC
Cuts at: 121 2286 3144 121
Size: 2165 858 1557
Fragments arranged by size:
2165 1557 858
EaeI y'GGCC_r
Cuts at: 2165 2339 2730 2757 4102 2165
Size: 174 391 27 1345 2643
Fragments arranged by size:
2643 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2165 2165
Size: 4580
EarI CTCTTCn'nnn_
Cuts at: 1059 2603 2813 1059
Size: 1544 210 2826
Fragments arranged by size:
2826 1544 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2788 3098 3244 4390 2788
Size: 310 146 1146 2978
Fragments arranged by size:
2978 1146 310 146
EcoICRI GAG'CTC
Cuts at: 160 1592 160
Size: 1432 3148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 428 602 781 1188 1418 1558 2405 2837
Size: 87 174 179 407 230 140 847 432
Cuts at: 2837 3584 3852 341
Size: 747 268 1069
Fragments arranged by size:
1069 847 747 432 407 268 230 179
174 140 87
EcoO109I rG'GnC_Cy
Cuts at: 1387 1719 1387
Size: 332 4248
EcoRI G'AATT_C
Cuts at: 243 1584 243
Size: 1341 3239
EcoRV GAT'ATC
Cuts at: 489 489
Size: 4580
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 522 2599 522
Size: 2077 2503
FspI TGC'GCA
Cuts at: 2361 2361
Size: 4580
HaeII r_GCGC'y
Cuts at: 2014 2262 3284 4263 2014
Size: 248 1022 979 2331
Fragments arranged by size:
2331 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 478 510 532 564 649 681 1513 1545 2422
Size: 32 22 32 85 32 832 32 877
Cuts at: 2422 2452 2454 2484 478
Size: 30 2 30 2574
Fragments arranged by size:
2574 877 832 85 32 32 32 32
30 30 22 2
HincII GTy'rAC
Cuts at: 1619 1619
Size: 4580
KasI G'GCGC_C
Cuts at: 2010 2258 2010
Size: 248 4332
KpnI G_GTAC'C
Cuts at: 1600 1600
Size: 4580
MluI A'CGCG_T
Cuts at: 4013 4013
Size: 4580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 706 3251 3435 706
Size: 2545 184 1851
Fragments arranged by size:
2545 1851 184
MscI TGG'CCA
Cuts at: 2341 2341
Size: 4580
MslI CAynn'nnrTG
Cuts at: 2569 2696 2569
Size: 127 4453
MspA1I CmG'CkG
Cuts at: 1691 2005 2365 3378 3623 1691
Size: 314 360 1013 245 2648
Fragments arranged by size:
2648 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2761 2761
Size: 4580
NarI GG'CG_CC
Cuts at: 2011 2259 2011
Size: 248 4332
NcoI C'CATG_G
Cuts at: 107 107
Size: 4580
NgoMIV G'CCGG_C
Cuts at: 2759 2759
Size: 4580
NsiI A_TGCA'T
Cuts at: 1191 1191
Size: 4580
NspI r_CATG'y
Cuts at: 1633 2133 3040 1633
Size: 500 907 3173
Fragments arranged by size:
3173 907 500
PciI A'CATG_T
Cuts at: 3036 3036
Size: 4580
PmeI GTTT'AAAC
Cuts at: 1579 1579
Size: 4580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 3719
Size: 32 4548
PsiI TTA'TAA
Cuts at: 644 644
Size: 4580
PstI C_TGCA'G
Cuts at: 1627 2312 1627
Size: 685 3895
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4580
PvuII CAG'CTG
Cuts at: 2005 2365 2005
Size: 360 4220
RsrII CG'GwC_CG
Cuts at: 2775 2775
Size: 4580
SacI G_AGCT'C
Cuts at: 162 1594 162
Size: 1432 3148
SalI G'TCGA_C
Cuts at: 1617 1617
Size: 4580
SapI GCTCTTCn'nnn_
Cuts at: 2603 2813 2603
Size: 210 4370
SbfI CC_TGCA'GG
Cuts at: 1627 1627
Size: 4580
ScaI AGT'ACT
Cuts at: 4097 4097
Size: 4580
SexAI A'CCwGG_T
Cuts at: 1362 1362
Size: 4580
SfcI C'TryA_G
Cuts at: 33 1623 2308 3301 3492 33
Size: 1590 685 993 191 1121
Fragments arranged by size:
1590 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2012 2260 2012
Size: 248 4332
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4580
SmaI CCC'GGG
Cuts at: 1602 1602
Size: 4580
SmlI C'TyrA_G
Cuts at: 700 866 1312 1465 1735 2650 3142 3404 700
Size: 166 446 153 270 915 492 262 1876
Fragments arranged by size:
1876 915 492 446 270 262 166 153
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4580
SphI G_CATG'C
Cuts at: 1633 2133 1633
Size: 500 4080
SspI AAT'ATT
Cuts at: 291 291
Size: 4580
StuI AGG'CCT
Cuts at: 1562 1562
Size: 4580
StyI C'CwwG_G
Cuts at: 107 444 668 1714 2068 107
Size: 337 224 1046 354 2619
Fragments arranged by size:
2619 1046 354 337 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1515 2163 2296 2755 2964 1515
Size: 648 133 459 209 3131
Fragments arranged by size:
3131 648 459 209 133
TatI w'GTAC_w
Cuts at: 1247 4095 1247
Size: 2848 1732
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4097 4129 4097
Size: 32 4548
Tth111I GACn'n_nGTC
Cuts at: 2377 2377
Size: 4580
XbaI T'CTAG_A
Cuts at: 1611 1611
Size: 4580
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 442 2075 442
Size: 1633 2947
XhoI C'TCGA_G
Cuts at: 866 866
Size: 4580
XmaI C'CCGG_G
Cuts at: 1600 1600
Size: 4580
XmnI GAAnn'nnTTC
Cuts at: 505 505
Size: 4580
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I BsaXI
BsgI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SwaI ZraI