Restriction map for
pF1KE0959
(Circular) (Six-base) MAPSORT of: pF1KE0959.seq Check: 3103 from: 1 to: 4610
pF1KE0959 4610 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1472 1472
Size: 4610
AccI GT'mk_AC
Cuts at: 1648 1648
Size: 4610
Acc65I G'GTAC_C
Cuts at: 1626 1626
Size: 4610
AclI AA'CG_TT
Cuts at: 4450 4450
Size: 4610
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2435 2867 3614 3882 2435
Size: 432 747 268 3163
Fragments arranged by size:
3163 747 432 268
AflIII A'CryG_T
Cuts at: 1962 3066 4043 1962
Size: 1104 977 2529
Fragments arranged by size:
2529 1104 977
AgeI A'CCGG_T
Cuts at: 2998 2998
Size: 4610
AlwNI CAG_nnn'CTG
Cuts at: 217 792 895 1050 1263 1326 1397 1915 3482
Size: 575 103 155 213 63 71 518 1567
Cuts at: 3482 217
Size: 1345
Fragments arranged by size:
1567 1345 575 518 213 155 103 71
63
ApaI G_GGCC'C
Cuts at: 550 564 683 836 1251 1333 550
Size: 14 119 153 415 82 3827
Fragments arranged by size:
3827 415 153 119 82 14
ApaLI G'TGCA_C
Cuts at: 796 1485 3380 796
Size: 689 1895 2026
Fragments arranged by size:
2026 1895 689
ApoI r'AATT_y
Cuts at: 1614 3963 4219 1614
Size: 2349 256 2005
Fragments arranged by size:
2349 2005 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4610
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4610
AvaI C'yCGr_G
Cuts at: 836 934 1535 1630 836
Size: 98 601 95 3816
Fragments arranged by size:
3816 601 98 95
AvrII C'CTAG_G
Cuts at: 1413 1413
Size: 4610
BamHI G'GATC_C
Cuts at: 1635 1635
Size: 4610
BanI G'GyrC_C
Cuts at: 154 175 329 712 1626 2040 2288 2323 154
Size: 21 154 383 914 414 248 35 2441
Fragments arranged by size:
2441 914 414 383 248 154 35 21
BanII G_rGCy'C
Cuts at: 248 391 550 564 683 836 952 1251 1333
Size: 143 159 14 119 153 116 299 82
Cuts at: 1333 1543 1624 2654 248
Size: 210 81 1030 2204
Fragments arranged by size:
2204 1030 299 210 159 153 143 119
116 82 81 14
BbeI G_GCGC'C
Cuts at: 2044 2292 2044
Size: 248 4362
BbsI GAAGACnn'nnnn_
Cuts at: 471 1085 471
Size: 614 3996
BbvCI CC'TCA_GC
Cuts at: 1573 1573
Size: 4610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4576
BciVI GTATCCnnnnn_n'
Cuts at: 2508 3269 4610 2508
Size: 761 1341 2508
Fragments arranged by size:
2508 1341 761
BclI T'GATC_A
Cuts at: 2130 2130
Size: 4610
BglI GCCn_nnn'nGGC
Cuts at: 243 455 1168 243
Size: 212 713 3685
Fragments arranged by size:
3685 713 212
BglII A'GATC_T
Cuts at: 2125 4162 2125
Size: 2037 2573
BlpI GC'TnA_GC
Cuts at: 247 445 1722 247
Size: 198 1277 3135
Fragments arranged by size:
3135 1277 198
Bme1580I G_kGCm'C
Cuts at: 157 550 564 683 800 836 1251 1333 1489
Size: 393 14 119 117 36 415 82 156
Cuts at: 1489 2235 2328 3384 157
Size: 746 93 1056 1383
Fragments arranged by size:
1383 1056 746 415 393 156 119 117
93 82 36 14
BmrI ACTGGGnnnn_n'
Cuts at: 181 1407 1822 1903 2238 4382 181
Size: 1226 415 81 335 2144 409
Fragments arranged by size:
2144 1226 415 409 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2977 2977
Size: 4610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 1147 1374 646
Size: 501 227 3882
Fragments arranged by size:
3882 501 227
Bpu10I CC'TnA_GC
Cuts at: 1234 1573 1234
Size: 339 4271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 1786 2665 3157 3455 214
Size: 1572 879 492 298 1369
Fragments arranged by size:
1572 1369 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 908 4314 908
Size: 3406 1204
BsaAI yAC'GTr
Cuts at: 1963 1963
Size: 4610
BsaBI GATnn'nnATC
Cuts at: 6 2124 4161 6
Size: 2118 2037 455
Fragments arranged by size:
2118 2037 455
BsaHI Gr'CG_yC
Cuts at: 2041 2289 2991 4487 2041
Size: 248 702 1496 2164
Fragments arranged by size:
2164 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1799 2320 2998 3272 3419 3830 1799
Size: 521 678 274 147 411 2579
Fragments arranged by size:
2579 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 302 655 1523 1561 302
Size: 353 868 38 3351
Fragments arranged by size:
3351 868 353 38
BseYI C'CCAG_C
Cuts at: 192 682 852 879 2035 2793 3370 3866 192
Size: 490 170 27 1156 758 577 496 936
Fragments arranged by size:
1156 936 758 577 496 490 170 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 763 110
Size: 653 3957
BsiEI CG_ry'CG
Cuts at: 104 507 1157 2152 2198 3406 104
Size: 403 650 995 46 1208 1308
Fragments arranged by size:
1308 1208 995 650 403 46
BsiHKAI G_wGCw'C
Cuts at: 800 1465 1489 1624 2402 2592 3384 800
Size: 665 24 135 778 190 792 2026
Fragments arranged by size:
2026 792 778 665 190 135 24
BsmI GAATG_Cn'
Cuts at: 287 287
Size: 4610
Bsp1286I G_dGCh'C
Cuts at: 157 248 391 550 564 683 800 836 952
Size: 91 143 159 14 119 117 36 116
Cuts at: 952 1251 1333 1465 1489 1543 1624 2235 2328
Size: 299 82 132 24 54 81 611 93
Cuts at: 2328 2402 2592 2654 3384 157
Size: 74 190 62 730 1383
Fragments arranged by size:
1383 730 611 299 190 159 143 132
119 117 116 93 91 82 81 74
62 54 36 24 14
BspEI T'CCGG_A
Cuts at: 3830 3830
Size: 4610
BspHI T'CATG_A
Cuts at: 1118 3786 1118
Size: 2668 1942
BspMI ACCTGCnnnn'nnnn_
Cuts at: 668 755 1073 1472 1660 1874 2176 2557 668
Size: 87 318 399 188 214 302 381 2721
Fragments arranged by size:
2721 399 381 318 302 214 188 87
BsrBI CCG'CTC
Cuts at: 50 2902 2956 4440 4605 50
Size: 2852 54 1484 165 55
Fragments arranged by size:
2852 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2522 2522
Size: 4610
BsrFI r'CCGG_y
Cuts at: 407 506 1084 2608 2789 2998 407
Size: 99 578 1524 181 209 2019
Fragments arranged by size:
2019 1524 578 209 181 99
BssSI C'ACGA_G
Cuts at: 589 2881 3239 589
Size: 2292 358 1960
Fragments arranged by size:
2292 1960 358
BstAPI GCAn_nnn'nTGC
Cuts at: 336 1050 1263 1987 336
Size: 714 213 724 2959
Fragments arranged by size:
2959 724 714 213
BstBI TT'CG_AA
Cuts at: 2971 2971
Size: 4610
BstYI r'GATC_y
Cuts at: 1 1635 2102 2125 2460 2706 3718 3804 3816
Size: 1634 467 23 335 246 1012 86 12
Cuts at: 3816 4162 1
Size: 346 449
Fragments arranged by size:
1634 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 725 1433 725
Size: 708 3902
BtgI C'CryG_G
Cuts at: 146 459 1026 1151 1192 146
Size: 313 567 125 41 3564
Fragments arranged by size:
3564 567 313 125 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2560 2741 2904 2560
Size: 181 163 4266
Fragments arranged by size:
4266 181 163
BtsI GCAGTG_nn'
Cuts at: 1079 1171 1853 1079
Size: 92 682 3836
Fragments arranged by size:
3836 682 92
DraI TTT'AAA
Cuts at: 1609 1609
Size: 4610
DrdI GACnn_nn'nnGTC
Cuts at: 2316 3174 2316
Size: 858 3752
EaeI y'GGCC_r
Cuts at: 131 409 504 572 1154 2195 2369 2760 2787
Size: 278 95 68 582 1041 174 391 27
Cuts at: 2787 4132 131
Size: 1345 609
Fragments arranged by size:
1345 1041 609 582 391 278 174 95
68 27
EagI C'GGCC_G
Cuts at: 504 1154 2195 504
Size: 650 1041 2919
Fragments arranged by size:
2919 1041 650
EarI CTCTTCn'nnn_
Cuts at: 310 2633 2843 310
Size: 2323 210 2077
Fragments arranged by size:
2323 2077 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 193 257 2818 3128 3274 4420 193
Size: 64 2561 310 146 1146 383
Fragments arranged by size:
2561 1146 383 310 146 64
EcoICRI GAG'CTC
Cuts at: 1622 1622
Size: 4610
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 1147 1374 2435 2867 3614 3882 646
Size: 501 227 1061 432 747 268 1374
Fragments arranged by size:
1374 1061 747 501 432 268 227
EcoNI CCTnn'n_nnAGG
Cuts at: 724 1255 1582 724
Size: 531 327 3752
Fragments arranged by size:
3752 531 327
EcoO109I rG'GnC_Cy
Cuts at: 546 547 789 832 833 1161 1230 1248 1260
Size: 1 242 43 1 328 69 18 12
Cuts at: 1260 1417 1749 546
Size: 157 332 3407
Fragments arranged by size:
3407 332 328 242 157 69 43 18
12 1 1
EcoRI G'AATT_C
Cuts at: 1614 1614
Size: 4610
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2629 2629
Size: 4610
FspI TGC'GCA
Cuts at: 2391 2391
Size: 4610
HaeII r_GCGC'y
Cuts at: 2044 2292 3314 4293 2044
Size: 248 1022 979 2361
Fragments arranged by size:
2361 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 212 244 1507 1539 2452 2482 2484 2514 212
Size: 32 1263 32 913 30 2 30 2308
Fragments arranged by size:
2308 1263 913 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1649 1649
Size: 4610
KasI G'GCGC_C
Cuts at: 2040 2288 2040
Size: 248 4362
KpnI G_GTAC'C
Cuts at: 1630 1630
Size: 4610
MluI A'CGCG_T
Cuts at: 4043 4043
Size: 4610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3281 3465 3281
Size: 184 4426
MscI TGG'CCA
Cuts at: 133 2371 133
Size: 2238 2372
MslI CAynn'nnrTG
Cuts at: 139 803 2599 2726 139
Size: 664 1796 127 2023
Fragments arranged by size:
2023 1796 664 127
MspA1I CmG'CkG
Cuts at: 336 845 967 1050 1194 1225 1313 1721 2035
Size: 509 122 83 144 31 88 408 314
Cuts at: 2035 2395 3408 3653 336
Size: 360 1013 245 1293
Fragments arranged by size:
1293 1013 509 408 360 314 245 144
122 88 83 31
NaeI GCC'GGC
Cuts at: 409 508 2791 409
Size: 99 2283 2228
Fragments arranged by size:
2283 2228 99
NarI GG'CG_CC
Cuts at: 2041 2289 2041
Size: 248 4362
NcoI C'CATG_G
Cuts at: 459 459
Size: 4610
NgoMIV G'CCGG_C
Cuts at: 407 506 2789 407
Size: 99 2283 2228
Fragments arranged by size:
2283 2228 99
NspI r_CATG'y
Cuts at: 1010 1663 2163 3070 1010
Size: 653 500 907 2550
Fragments arranged by size:
2550 907 653 500
PasI CC'CwG_GG
Cuts at: 239 391 1326 1493 239
Size: 152 935 167 3356
Fragments arranged by size:
3356 935 167 152
PciI A'CATG_T
Cuts at: 3066 3066
Size: 4610
PflMI CCAn_nnn'nTGG
Cuts at: 792 1397 792
Size: 605 4005
PfoI T'CCnGG_A
Cuts at: 905 995 1373 905
Size: 90 378 4142
Fragments arranged by size:
4142 378 90
PmeI GTTT'AAAC
Cuts at: 1609 1609
Size: 4610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 3749
Size: 32 4578
PspOMI G'GGCC_C
Cuts at: 546 560 679 832 1247 1329 546
Size: 14 119 153 415 82 3827
Fragments arranged by size:
3827 415 153 119 82 14
PstI C_TGCA'G
Cuts at: 341 386 665 692 947 1366 1657 2342 341
Size: 45 279 27 255 419 291 685 2609
Fragments arranged by size:
2609 685 419 291 279 255 45 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4610
PvuII CAG'CTG
Cuts at: 336 1050 1313 2035 2395 336
Size: 714 263 722 360 2551
Fragments arranged by size:
2551 722 714 360 263
RsrII CG'GwC_CG
Cuts at: 2805 2805
Size: 4610
SacI G_AGCT'C
Cuts at: 1624 1624
Size: 4610
SacII CC_GC'GG
Cuts at: 1195 1195
Size: 4610
SalI G'TCGA_C
Cuts at: 1647 1647
Size: 4610
SapI GCTCTTCn'nnn_
Cuts at: 2633 2843 2633
Size: 210 4400
SbfI CC_TGCA'GG
Cuts at: 1657 1657
Size: 4610
ScaI AGT'ACT
Cuts at: 4127 4127
Size: 4610
SfcI C'TryA_G
Cuts at: 33 337 382 661 688 943 1318 1362 1653
Size: 304 45 279 27 255 375 44 291
Cuts at: 1653 2338 3331 3522 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 375 304 291 279 255
191 45 44 27
SfoI GGC'GCC
Cuts at: 2042 2290 2042
Size: 248 4362
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4610
SmaI CCC'GGG
Cuts at: 838 1632 838
Size: 794 3816
SmlI C'TyrA_G
Cuts at: 229 1765 2680 3172 3434 229
Size: 1536 915 492 262 1405
Fragments arranged by size:
1536 1405 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4610
SphI G_CATG'C
Cuts at: 1010 1663 2163 1010
Size: 653 500 3457
Fragments arranged by size:
3457 653 500
StuI AGG'CCT
Cuts at: 370 634 706 370
Size: 264 72 4274
Fragments arranged by size:
4274 264 72
StyI C'CwwG_G
Cuts at: 278 459 494 551 1413 1744 2098 278
Size: 181 35 57 862 331 354 2790
Fragments arranged by size:
2790 862 354 331 181 57 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 623 844 1506 2193 2326 2785 2994 623
Size: 221 662 687 133 459 209 2239
Fragments arranged by size:
2239 687 662 459 221 209 133
TatI w'GTAC_w
Cuts at: 4125 4125
Size: 4610
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 857 889 4127 4159 857
Size: 32 3238 32 1308
Fragments arranged by size:
3238 1308 32 32
Tth111I GACn'n_nGTC
Cuts at: 2407 2407
Size: 4610
XbaI T'CTAG_A
Cuts at: 1641 1641
Size: 4610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 214 825 999 1500 2105 214
Size: 611 174 501 605 2719
Fragments arranged by size:
2719 611 605 501 174
XmaI C'CCGG_G
Cuts at: 836 1630 836
Size: 794 3816
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
BaeI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PpuMI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI