Restriction map for
pF1KE0952
(Circular) (Six-base) MAPSORT of: pF1KE0952.seq Check: 825 from: 1 to: 4535
pF1KE0952 4535 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 951 951
Size: 4535
AccI GT'mk_AC
Cuts at: 1573 1573
Size: 4535
Acc65I G'GTAC_C
Cuts at: 1551 1551
Size: 4535
AclI AA'CG_TT
Cuts at: 4375 4375
Size: 4535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 547 631 889 1270 1423 2360 2792 3539
Size: 164 84 258 381 153 937 432 747
Cuts at: 3539 3807 383
Size: 268 1111
Fragments arranged by size:
1111 937 747 432 381 268 258 164
153 84
AflIII A'CryG_T
Cuts at: 228 1122 1887 2991 3968 228
Size: 894 765 1104 977 795
Fragments arranged by size:
1104 977 894 795 765
AgeI A'CCGG_T
Cuts at: 2923 2923
Size: 4535
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1204 1204
Size: 4535
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1156 1188 1156
Size: 32 4503
AlwNI CAG_nnn'CTG
Cuts at: 525 1233 1485 1840 3407 525
Size: 708 252 355 1567 1653
Fragments arranged by size:
1653 1567 708 355 252
ApaI G_GGCC'C
Cuts at: 1208 1346 1208
Size: 138 4397
ApaLI G'TGCA_C
Cuts at: 3305 3305
Size: 4535
ApoI r'AATT_y
Cuts at: 157 177 1539 3888 4144 157
Size: 20 1362 2349 256 548
Fragments arranged by size:
2349 1362 548 256 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 4535
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
AvaI C'yCGr_G
Cuts at: 1555 1555
Size: 4535
BamHI G'GATC_C
Cuts at: 1050 1560 1050
Size: 510 4025
BanI G'GyrC_C
Cuts at: 1524 1551 1965 2213 2248 1524
Size: 27 414 248 35 3811
Fragments arranged by size:
3811 414 248 35 27
BanII G_rGCy'C
Cuts at: 454 517 1153 1208 1346 1549 2579 454
Size: 63 636 55 138 203 1030 2410
Fragments arranged by size:
2410 1030 636 203 138 63 55
BbeI G_GCGC'C
Cuts at: 1969 2217 1969
Size: 248 4287
BbsI GAAGACnn'nnnn_
Cuts at: 445 487 508 445
Size: 42 21 4472
Fragments arranged by size:
4472 42 21
BbvCI CC'TCA_GC
Cuts at: 1453 1453
Size: 4535
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 801 828 835 862 801
Size: 27 7 27 4474
Fragments arranged by size:
4474 27 27 7
BciVI GTATCCnnnnn_n'
Cuts at: 151 2433 3194 4535 151
Size: 2282 761 1341 151
Fragments arranged by size:
2282 1341 761 151
BclI T'GATC_A
Cuts at: 2055 2055
Size: 4535
BglI GCCn_nnn'nGGC
Cuts at: 1115 1115
Size: 4535
BglII A'GATC_T
Cuts at: 2050 4087 2050
Size: 2037 2498
BlpI GC'TnA_GC
Cuts at: 1647 1647
Size: 4535
Bme1580I G_kGCm'C
Cuts at: 1208 1346 1529 2160 2253 3309 1208
Size: 138 183 631 93 1056 2434
Fragments arranged by size:
2434 1056 631 183 138 93
BmrI ACTGGGnnnn_n'
Cuts at: 1747 1828 2163 4307 1747
Size: 81 335 2144 1975
Fragments arranged by size:
2144 1975 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 4535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 1009 1210 252
Size: 757 201 3577
Fragments arranged by size:
3577 757 201
Bpu10I CC'TnA_GC
Cuts at: 1453 1453
Size: 4535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1189 1711 2590 3082 3380 1189
Size: 522 879 492 298 2344
Fragments arranged by size:
2344 879 522 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1378 4239 1378
Size: 2861 1674
BsaAI yAC'GTr
Cuts at: 1125 1134 1424 1888 1125
Size: 9 290 464 3772
Fragments arranged by size:
3772 464 290 9
BsaBI GATnn'nnATC
Cuts at: 6 2049 4086 6
Size: 2043 2037 455
Fragments arranged by size:
2043 2037 455
BsaHI Gr'CG_yC
Cuts at: 1966 2214 2916 4412 1966
Size: 248 702 1496 2089
Fragments arranged by size:
2089 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1724 2245 2923 3197 3344 3755 1724
Size: 521 678 274 147 411 2504
Fragments arranged by size:
2504 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 912 1444 208
Size: 704 532 3299
Fragments arranged by size:
3299 704 532
BseYI C'CCAG_C
Cuts at: 886 1960 2718 3295 3791 886
Size: 1074 758 577 496 1630
Fragments arranged by size:
1630 1074 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 641 1174 641
Size: 533 4002
BsiEI CG_ry'CG
Cuts at: 104 2077 2123 3331 104
Size: 1973 46 1208 1308
Fragments arranged by size:
1973 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1549 2327 2517 3309 1549
Size: 778 190 792 2775
Fragments arranged by size:
2775 792 778 190
BsmI GAATG_Cn'
Cuts at: 151 641 151
Size: 490 4045
Bsp1286I G_dGCh'C
Cuts at: 454 517 1153 1208 1346 1529 1549 2160 2253
Size: 63 636 55 138 183 20 611 93
Cuts at: 2253 2327 2517 2579 3309 454
Size: 74 190 62 730 1680
Fragments arranged by size:
1680 730 636 611 190 183 138 93
74 63 62 55 20
BspEI T'CCGG_A
Cuts at: 3755 3755
Size: 4535
BspHI T'CATG_A
Cuts at: 1307 3711 1307
Size: 2404 2131
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 951 1196 1585 1799 2101 2482 844
Size: 107 245 389 214 302 381 2897
Fragments arranged by size:
2897 389 381 302 245 214 107
BsrBI CCG'CTC
Cuts at: 50 2827 2881 4365 4530 50
Size: 2777 54 1484 165 55
Fragments arranged by size:
2777 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2447 2447
Size: 4535
BsrFI r'CCGG_y
Cuts at: 971 2533 2714 2923 971
Size: 1562 181 209 2583
Fragments arranged by size:
2583 1562 209 181
BssSI C'ACGA_G
Cuts at: 1156 2806 3164 1156
Size: 1650 358 2527
Fragments arranged by size:
2527 1650 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1485 1912 1485
Size: 427 4108
BstBI TT'CG_AA
Cuts at: 2896 2896
Size: 4535
BstEII G'GTnAC_C
Cuts at: 459 459
Size: 4535
BstYI r'GATC_y
Cuts at: 1 388 1050 1482 1560 2027 2050 2385 2631
Size: 387 662 432 78 467 23 335 246
Cuts at: 2631 3643 3729 3741 4087 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 662 467 449 432 387 346 335
246 86 78 23 12
Bsu36I CC'TnA_GG
Cuts at: 455 455
Size: 4535
BtgI C'CryG_G
Cuts at: 1273 1273
Size: 4535
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2485 2666 2829 2485
Size: 181 163 4191
Fragments arranged by size:
4191 181 163
BtsI GCAGTG_nn'
Cuts at: 817 1778 817
Size: 961 3574
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 534 569 534
Size: 35 4500
DraI TTT'AAA
Cuts at: 1534 1534
Size: 4535
DrdI GACnn_nn'nnGTC
Cuts at: 1361 2241 3099 1361
Size: 880 858 2797
Fragments arranged by size:
2797 880 858
EaeI y'GGCC_r
Cuts at: 824 2120 2294 2685 2712 4057 824
Size: 1296 174 391 27 1345 1302
Fragments arranged by size:
1345 1302 1296 391 174 27
EagI C'GGCC_G
Cuts at: 2120 2120
Size: 4535
EarI CTCTTCn'nnn_
Cuts at: 340 442 2558 2768 340
Size: 102 2116 210 2107
Fragments arranged by size:
2116 2107 210 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2743 3053 3199 4345 2743
Size: 310 146 1146 2933
Fragments arranged by size:
2933 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1547 1547
Size: 4535
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 383 547 631 889 1009 1210 1270 1423
Size: 131 164 84 258 120 201 60 153
Cuts at: 1423 2360 2792 3539 3807 252
Size: 937 432 747 268 980
Fragments arranged by size:
980 937 747 432 268 258 201 164
153 131 120 84 60
EcoNI CCTnn'n_nnAGG
Cuts at: 1192 1192
Size: 4535
EcoO109I rG'GnC_Cy
Cuts at: 206 1164 1205 1342 1343 1354 1376 1674 206
Size: 958 41 137 1 11 22 298 3067
Fragments arranged by size:
3067 958 298 137 41 22 11 1
EcoRI G'AATT_C
Cuts at: 1539 1539
Size: 4535
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2554 2554
Size: 4535
FspI TGC'GCA
Cuts at: 2316 2316
Size: 4535
HaeII r_GCGC'y
Cuts at: 738 1969 2217 3239 4218 738
Size: 1231 248 1022 979 1055
Fragments arranged by size:
1231 1055 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 317 349 2377 2407 2409 2439 317
Size: 32 2028 30 2 30 2413
Fragments arranged by size:
2413 2028 32 30 30 2
HincII GTy'rAC
Cuts at: 789 1574 789
Size: 785 3750
HindIII A'AGCT_T
Cuts at: 557 1469 557
Size: 912 3623
KasI G'GCGC_C
Cuts at: 1965 2213 1965
Size: 248 4287
KpnI G_GTAC'C
Cuts at: 1555 1555
Size: 4535
MluI A'CGCG_T
Cuts at: 3968 3968
Size: 4535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3206 3390 3206
Size: 184 4351
MscI TGG'CCA
Cuts at: 826 2296 826
Size: 1470 3065
MslI CAynn'nnrTG
Cuts at: 1004 1022 1180 2524 2651 1004
Size: 18 158 1344 127 2888
Fragments arranged by size:
2888 1344 158 127 18
MspA1I CmG'CkG
Cuts at: 488 765 1033 1646 1960 2320 3333 3578 488
Size: 277 268 613 314 360 1013 245 1445
Fragments arranged by size:
1445 1013 613 360 314 277 268 245
NaeI GCC'GGC
Cuts at: 973 2716 973
Size: 1743 2792
NarI GG'CG_CC
Cuts at: 1966 2214 1966
Size: 248 4287
NcoI C'CATG_G
Cuts at: 1273 1273
Size: 4535
NdeI CA'TA_TG
Cuts at: 1500 1500
Size: 4535
NgoMIV G'CCGG_C
Cuts at: 971 2714 971
Size: 1743 2792
NspI r_CATG'y
Cuts at: 202 232 937 1248 1588 2088 2995 202
Size: 30 705 311 340 500 907 1742
Fragments arranged by size:
1742 907 705 500 340 311 30
PciI A'CATG_T
Cuts at: 228 2991 228
Size: 2763 1772
PflMI CCAn_nnn'nTGG
Cuts at: 130 1233 130
Size: 1103 3432
PfoI T'CCnGG_A
Cuts at: 400 400
Size: 4535
PmeI GTTT'AAAC
Cuts at: 1534 1534
Size: 4535
PmlI CAC'GTG
Cuts at: 1125 1125
Size: 4535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 3674
Size: 32 4503
PpuMI rG'GwC_Cy
Cuts at: 206 1164 1354 206
Size: 958 190 3387
Fragments arranged by size:
3387 958 190
PshAI GACnn'nnGTC
Cuts at: 217 1064 217
Size: 847 3688
PspOMI G'GGCC_C
Cuts at: 1204 1342 1204
Size: 138 4397
PstI C_TGCA'G
Cuts at: 962 1394 1481 1582 2267 962
Size: 432 87 101 685 3230
Fragments arranged by size:
3230 685 432 101 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4535
PvuII CAG'CTG
Cuts at: 488 1033 1960 2320 488
Size: 545 927 360 2703
Fragments arranged by size:
2703 927 545 360
RsrII CG'GwC_CG
Cuts at: 2730 2730
Size: 4535
SacI G_AGCT'C
Cuts at: 1549 1549
Size: 4535
SalI G'TCGA_C
Cuts at: 1572 1572
Size: 4535
SanDI GG'GwC_CC
Cuts at: 206 1354 206
Size: 1148 3387
SapI GCTCTTCn'nnn_
Cuts at: 442 2558 2768 442
Size: 2116 210 2209
Fragments arranged by size:
2209 2116 210
SbfI CC_TGCA'GG
Cuts at: 1582 1582
Size: 4535
ScaI AGT'ACT
Cuts at: 4052 4052
Size: 4535
SfcI C'TryA_G
Cuts at: 33 188 489 958 1390 1477 1578 2263 3256
Size: 155 301 469 432 87 101 685 993
Cuts at: 3256 3447 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 469 432 301 191 155
101 87
SfoI GGC'GCC
Cuts at: 1967 2215 1967
Size: 248 4287
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
SmaI CCC'GGG
Cuts at: 1557 1557
Size: 4535
SmlI C'TyrA_G
Cuts at: 1168 1690 2605 3097 3359 1168
Size: 522 915 492 262 2344
Fragments arranged by size:
2344 915 522 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4535
SphI G_CATG'C
Cuts at: 1248 1588 2088 1248
Size: 340 500 3695
Fragments arranged by size:
3695 500 340
StyI C'CwwG_G
Cuts at: 243 725 1273 1669 2023 243
Size: 482 548 396 354 2755
Fragments arranged by size:
2755 548 482 396 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 675 2118 2251 2710 2919 675
Size: 1443 133 459 209 2291
Fragments arranged by size:
2291 1443 459 209 133
TatI w'GTAC_w
Cuts at: 806 4050 806
Size: 3244 1291
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4052 4084 4052
Size: 32 4503
Tth111I GACn'n_nGTC
Cuts at: 2332 2332
Size: 4535
XbaI T'CTAG_A
Cuts at: 1566 1566
Size: 4535
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2030 2030
Size: 4535
XmaI C'CCGG_G
Cuts at: 1555 1555
Size: 4535
XmnI GAAnn'nnTTC
Cuts at: 616 757 616
Size: 141 4394
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BaeI
BfrBI BmgBI BmtI BsaXI BsiWI BsmBI BsrGI BssHII
BstXI BstZ17I ClaI DraIII EcoRV FseI FspAI HpaI
MfeI NheI NotI NruI NsiI PacI PasI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI