Restriction map for
pF1KE0948
(Circular) (Six-base) MAPSORT of: pF1KE0948.seq Check: 6928 from: 1 to: 4391
pF1KE0948 4391 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 209 1429 209
Size: 1220 3171
Acc65I G'GTAC_C
Cuts at: 1407 1407
Size: 4391
AclI AA'CG_TT
Cuts at: 4231 4231
Size: 4391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 922 1260 2216 2648 3395 3663 502
Size: 420 338 956 432 747 268 1230
Fragments arranged by size:
1230 956 747 432 420 338 268
AfeI AGC'GCT
Cuts at: 973 973
Size: 4391
AflIII A'CryG_T
Cuts at: 1743 2847 3824 1743
Size: 1104 977 2310
Fragments arranged by size:
2310 1104 977
AgeI A'CCGG_T
Cuts at: 2779 2779
Size: 4391
AhdI GACnn_n'nnGTC
Cuts at: 702 702
Size: 4391
AleI CACnn'nnGTG
Cuts at: 631 631
Size: 4391
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 500 532 500
Size: 32 4359
AlwNI CAG_nnn'CTG
Cuts at: 1179 1696 3263 1179
Size: 517 1567 2307
Fragments arranged by size:
2307 1567 517
ApaI G_GGCC'C
Cuts at: 1057 1057
Size: 4391
ApaLI G'TGCA_C
Cuts at: 1324 3161 1324
Size: 1837 2554
ApoI r'AATT_y
Cuts at: 1395 3744 4000 1395
Size: 2349 256 1786
Fragments arranged by size:
2349 1786 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4391
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
AvaI C'yCGr_G
Cuts at: 143 1411 143
Size: 1268 3123
BamHI G'GATC_C
Cuts at: 1416 1416
Size: 4391
BanI G'GyrC_C
Cuts at: 637 654 1407 1821 2069 2104 637
Size: 17 753 414 248 35 2924
Fragments arranged by size:
2924 753 414 248 35 17
BanII G_rGCy'C
Cuts at: 164 196 899 956 1057 1151 1214 1405 2435
Size: 32 703 57 101 94 63 191 1030
Cuts at: 2435 164
Size: 2120
Fragments arranged by size:
2120 1030 703 191 101 94 63 57
32
BbeI G_GCGC'C
Cuts at: 1825 2073 1825
Size: 248 4143
BbsI GAAGACnn'nnnn_
Cuts at: 940 1194 940
Size: 254 4137
BciVI GTATCCnnnnn_n'
Cuts at: 2289 3050 4391 2289
Size: 761 1341 2289
Fragments arranged by size:
2289 1341 761
BclI T'GATC_A
Cuts at: 1911 1911
Size: 4391
BglII A'GATC_T
Cuts at: 1906 3943 1906
Size: 2037 2354
BlpI GC'TnA_GC
Cuts at: 411 1503 411
Size: 1092 3299
Bme1580I G_kGCm'C
Cuts at: 640 1057 1328 2016 2109 3165 640
Size: 417 271 688 93 1056 1866
Fragments arranged by size:
1866 1056 688 417 271 93
BmgBI CAC'GTC
Cuts at: 567 567
Size: 4391
BmrI ACTGGGnnnn_n'
Cuts at: 1167 1603 1684 2019 4163 1167
Size: 436 81 335 2144 1395
Fragments arranged by size:
2144 1395 436 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1023 2758 1023
Size: 1735 2656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 463 832 1276 1285 286
Size: 177 369 444 9 3392
Fragments arranged by size:
3392 444 369 177 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1567 2446 2938 3236 1567
Size: 879 492 298 2722
Fragments arranged by size:
2722 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4095 4095
Size: 4391
BsaAI yAC'GTr
Cuts at: 1744 1744
Size: 4391
BsaBI GATnn'nnATC
Cuts at: 6 1905 3942 6
Size: 1899 2037 455
Fragments arranged by size:
2037 1899 455
BsaHI Gr'CG_yC
Cuts at: 1822 2070 2772 4268 1822
Size: 248 702 1496 1945
Fragments arranged by size:
1945 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1580 2101 2779 3053 3200 3611 1580
Size: 521 678 274 147 411 2360
Fragments arranged by size:
2360 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 340 370 1233 1263 340
Size: 30 863 30 3468
Fragments arranged by size:
3468 863 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 172 283 460 1012 1273 1303 172
Size: 111 177 552 261 30 3260
Fragments arranged by size:
3260 552 261 177 111 30
BseYI C'CCAG_C
Cuts at: 1816 2574 3151 3647 1816
Size: 758 577 496 2560
Fragments arranged by size:
2560 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 1108 1363 484
Size: 624 255 3512
Fragments arranged by size:
3512 624 255
BsiEI CG_ry'CG
Cuts at: 104 180 963 1087 1933 1979 3187 104
Size: 76 783 124 846 46 1208 1308
Fragments arranged by size:
1308 1208 846 783 124 76 46
BsiHKAI G_wGCw'C
Cuts at: 164 373 899 956 1214 1328 1405 2183 2373
Size: 209 526 57 258 114 77 778 190
Cuts at: 2373 3165 164
Size: 792 1390
Fragments arranged by size:
1390 792 778 526 258 209 190 114
77 57
Bsp1286I G_dGCh'C
Cuts at: 164 196 373 640 899 956 1057 1151 1214
Size: 32 177 267 259 57 101 94 63
Cuts at: 1214 1328 1405 2016 2109 2183 2373 2435 3165
Size: 114 77 611 93 74 190 62 730
Cuts at: 3165 164
Size: 1390
Fragments arranged by size:
1390 730 611 267 259 190 177 114
101 94 93 77 74 63 62 57
32
BspEI T'CCGG_A
Cuts at: 3611 3611
Size: 4391
BspHI T'CATG_A
Cuts at: 3567 3567
Size: 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1441 1655 1957 2338 1441
Size: 214 302 381 3494
Fragments arranged by size:
3494 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1235 2683 2737 4221 4386 50
Size: 1185 1448 54 1484 165 55
Fragments arranged by size:
1484 1448 1185 165 55 54
BsrDI GCAATG_nn'
Cuts at: 455 2303 455
Size: 1848 2543
BsrFI r'CCGG_y
Cuts at: 2389 2570 2779 2389
Size: 181 209 4001
Fragments arranged by size:
4001 209 181
BssSI C'ACGA_G
Cuts at: 2662 3020 2662
Size: 358 4033
BstAPI GCAn_nnn'nTGC
Cuts at: 1768 1768
Size: 4391
BstBI TT'CG_AA
Cuts at: 2752 2752
Size: 4391
BstXI CCAn_nnnn'nTGG
Cuts at: 729 729
Size: 4391
BstYI r'GATC_y
Cuts at: 1 515 1416 1883 1906 2241 2487 3499 3585
Size: 514 901 467 23 335 246 1012 86
Cuts at: 3585 3597 3943 1
Size: 12 346 449
Fragments arranged by size:
1012 901 514 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 155 659 155
Size: 504 3887
BtgI C'CryG_G
Cuts at: 107 884 107
Size: 777 3614
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2341 2522 2685 2341
Size: 181 163 4047
Fragments arranged by size:
4047 181 163
BtsI GCAGTG_nn'
Cuts at: 1634 1634
Size: 4391
ClaI AT'CG_AT
Cuts at: 221 221
Size: 4391
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 221 256 221
Size: 35 4356
DraI TTT'AAA
Cuts at: 1390 1390
Size: 4391
DraIII CAC_nnn'GTG
Cuts at: 1332 1332
Size: 4391
DrdI GACnn_nn'nnGTC
Cuts at: 2097 2955 2097
Size: 858 3533
EaeI y'GGCC_r
Cuts at: 245 731 887 1084 1283 1976 2150 2541 2568
Size: 486 156 197 199 693 174 391 27
Cuts at: 2568 3913 245
Size: 1345 723
Fragments arranged by size:
1345 723 693 486 391 199 197 174
156 27
EagI C'GGCC_G
Cuts at: 1084 1976 1084
Size: 892 3499
EarI CTCTTCn'nnn_
Cuts at: 169 2414 2624 169
Size: 2245 210 1936
Fragments arranged by size:
2245 1936 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 592 1051 2599 2909 3055 4201 592
Size: 459 1548 310 146 1146 782
Fragments arranged by size:
1548 1146 782 459 310 146
EcoICRI GAG'CTC
Cuts at: 162 897 954 1212 1403 162
Size: 735 57 258 191 3150
Fragments arranged by size:
3150 735 258 191 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 463 502 832 922 1260 1276 1285 2216
Size: 177 39 330 90 338 16 9 931
Cuts at: 2216 2648 3395 3663 286
Size: 432 747 268 1014
Fragments arranged by size:
1014 931 747 432 338 330 268 177
90 39 16 9
EcoNI CCTnn'n_nnAGG
Cuts at: 268 1285 268
Size: 1017 3374
EcoO109I rG'GnC_Cy
Cuts at: 436 486 1053 1054 1169 1530 436
Size: 50 567 1 115 361 3297
Fragments arranged by size:
3297 567 361 115 50 1
EcoRI G'AATT_C
Cuts at: 1395 1395
Size: 4391
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 957 2410 957
Size: 1453 2938
FspI TGC'GCA
Cuts at: 2172 2172
Size: 4391
HaeII r_GCGC'y
Cuts at: 975 1825 2073 3095 4074 975
Size: 850 248 1022 979 1292
Fragments arranged by size:
1292 1022 979 850 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 688 720 2233 2263 2265 2295 688
Size: 32 1513 30 2 30 2784
Fragments arranged by size:
2784 1513 32 30 30 2
HincII GTy'rAC
Cuts at: 1430 1430
Size: 4391
HindIII A'AGCT_T
Cuts at: 1102 1102
Size: 4391
KasI G'GCGC_C
Cuts at: 1821 2069 1821
Size: 248 4143
KpnI G_GTAC'C
Cuts at: 1411 1411
Size: 4391
MluI A'CGCG_T
Cuts at: 3824 3824
Size: 4391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3062 3246 3062
Size: 184 4207
MscI TGG'CCA
Cuts at: 247 733 2152 247
Size: 486 1419 2486
Fragments arranged by size:
2486 1419 486
MslI CAynn'nnrTG
Cuts at: 631 2380 2507 631
Size: 1749 127 2515
Fragments arranged by size:
2515 1749 127
MspA1I CmG'CkG
Cuts at: 1502 1816 2176 3189 3434 1502
Size: 314 360 1013 245 2459
Fragments arranged by size:
2459 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2572 2572
Size: 4391
NarI GG'CG_CC
Cuts at: 1822 2070 1822
Size: 248 4143
NcoI C'CATG_G
Cuts at: 107 107
Size: 4391
NgoMIV G'CCGG_C
Cuts at: 2570 2570
Size: 4391
NspI r_CATG'y
Cuts at: 1444 1944 2851 1444
Size: 500 907 2984
Fragments arranged by size:
2984 907 500
PasI CC'CwG_GG
Cuts at: 708 708
Size: 4391
PciI A'CATG_T
Cuts at: 2847 2847
Size: 4391
PflMI CCAn_nnn'nTGG
Cuts at: 380 380
Size: 4391
PmeI GTTT'AAAC
Cuts at: 1390 1390
Size: 4391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 3530
Size: 32 4359
PpuMI rG'GwC_Cy
Cuts at: 436 1169 436
Size: 733 3658
PspOMI G'GGCC_C
Cuts at: 1053 1053
Size: 4391
PstI C_TGCA'G
Cuts at: 1438 2123 1438
Size: 685 3706
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4391
PvuII CAG'CTG
Cuts at: 1816 2176 1816
Size: 360 4031
RsrII CG'GwC_CG
Cuts at: 2586 2586
Size: 4391
SacI G_AGCT'C
Cuts at: 164 899 956 1214 1405 164
Size: 735 57 258 191 3150
Fragments arranged by size:
3150 735 258 191 57
SalI G'TCGA_C
Cuts at: 1428 1428
Size: 4391
SapI GCTCTTCn'nnn_
Cuts at: 169 2414 2624 169
Size: 2245 210 1936
Fragments arranged by size:
2245 1936 210
SbfI CC_TGCA'GG
Cuts at: 1438 1438
Size: 4391
ScaI AGT'ACT
Cuts at: 3908 3908
Size: 4391
SexAI A'CCwGG_T
Cuts at: 432 1328 432
Size: 896 3495
SfcI C'TryA_G
Cuts at: 33 1434 2119 3112 3303 33
Size: 1401 685 993 191 1121
Fragments arranged by size:
1401 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1823 2071 1823
Size: 248 4143
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
SmaI CCC'GGG
Cuts at: 1413 1413
Size: 4391
SmlI C'TyrA_G
Cuts at: 143 1546 2461 2953 3215 143
Size: 1403 915 492 262 1319
Fragments arranged by size:
1403 1319 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4391
SphI G_CATG'C
Cuts at: 1444 1944 1444
Size: 500 3891
StuI AGG'CCT
Cuts at: 853 853
Size: 4391
StyI C'CwwG_G
Cuts at: 107 734 890 1525 1879 107
Size: 627 156 635 354 2619
Fragments arranged by size:
2619 635 627 354 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 819 1237 1974 2107 2566 2775 819
Size: 418 737 133 459 209 2435
Fragments arranged by size:
2435 737 459 418 209 133
TatI w'GTAC_w
Cuts at: 3906 3906
Size: 4391
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1233 1265 1360 1392 3908 3940 1233
Size: 32 95 32 2516 32 1684
Fragments arranged by size:
2516 1684 95 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2188 2188
Size: 4391
XbaI T'CTAG_A
Cuts at: 1422 1422
Size: 4391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1886 1886
Size: 4391
XhoI C'TCGA_G
Cuts at: 143 143
Size: 4391
XmaI C'CCGG_G
Cuts at: 1411 1411
Size: 4391
XmnI GAAnn'nnTTC
Cuts at: 628 1273 628
Size: 645 3746
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AlfI AscI AvrII BaeI BbvCI
BcgI BfrBI BglI BmtI Bpu10I BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI ZraI