Restriction map for
pF1KE0946
(Circular) (Six-base) MAPSORT of: pF1KE0946.seq Check: 7859 from: 1 to: 4214
pF1KE0946 4214 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AatII G_ACGT'C
Cuts at: 503 878 503
Size: 375 3839
AccI GT'mk_AC
Cuts at: 365 1153 1252 365
Size: 788 99 3327
Fragments arranged by size:
3327 788 99
Acc65I G'GTAC_C
Cuts at: 1230 1230
Size: 4214
AclI AA'CG_TT
Cuts at: 4054 4054
Size: 4214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 555 2039 2471 3218 3486 229
Size: 326 1484 432 747 268 957
Fragments arranged by size:
1484 957 747 432 326 268
AfeI AGC'GCT
Cuts at: 586 586
Size: 4214
AflIII A'CryG_T
Cuts at: 1566 2670 3647 1566
Size: 1104 977 2133
Fragments arranged by size:
2133 1104 977
AgeI A'CCGG_T
Cuts at: 2602 2602
Size: 4214
AhdI GACnn_n'nnGTC
Cuts at: 1026 1026
Size: 4214
AlwNI CAG_nnn'CTG
Cuts at: 914 1519 3086 914
Size: 605 1567 2042
Fragments arranged by size:
2042 1567 605
ApaI G_GGCC'C
Cuts at: 1178 1206 1178
Size: 28 4186
ApaLI G'TGCA_C
Cuts at: 2984 2984
Size: 4214
ApoI r'AATT_y
Cuts at: 156 271 1218 3567 3823 156
Size: 115 947 2349 256 547
Fragments arranged by size:
2349 947 547 256 115
AseI AT'TA_AT
Cuts at: 20 20
Size: 4214
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4214
AvaI C'yCGr_G
Cuts at: 281 403 419 980 1090 1234 281
Size: 122 16 561 110 144 3261
Fragments arranged by size:
3261 561 144 122 110 16
AvrII C'CTAG_G
Cuts at: 711 711
Size: 4214
BamHI G'GATC_C
Cuts at: 1239 1239
Size: 4214
BanI G'GyrC_C
Cuts at: 1230 1644 1892 1927 1230
Size: 414 248 35 3517
Fragments arranged by size:
3517 414 248 35
BanII G_rGCy'C
Cuts at: 172 383 429 1178 1206 1228 2258 172
Size: 211 46 749 28 22 1030 2128
Fragments arranged by size:
2128 1030 749 211 46 28 22
BbeI G_GCGC'C
Cuts at: 1648 1896 1648
Size: 248 3966
BbvCI CC'TCA_GC
Cuts at: 907 907
Size: 4214
BciVI GTATCCnnnnn_n'
Cuts at: 796 2112 2873 4214 796
Size: 1316 761 1341 796
Fragments arranged by size:
1341 1316 796 761
BclI T'GATC_A
Cuts at: 1734 1734
Size: 4214
BglII A'GATC_T
Cuts at: 338 1729 3766 338
Size: 1391 2037 786
Fragments arranged by size:
2037 1391 786
BlpI GC'TnA_GC
Cuts at: 1326 1326
Size: 4214
Bme1580I G_kGCm'C
Cuts at: 1178 1206 1839 1932 2988 1178
Size: 28 633 93 1056 2404
Fragments arranged by size:
2404 1056 633 93 28
BmrI ACTGGGnnnn_n'
Cuts at: 1426 1507 1842 3986 1426
Size: 81 335 2144 1654
Fragments arranged by size:
2144 1654 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 160 2581 160
Size: 2421 1793
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 726 1063 1175 726
Size: 337 112 3765
Fragments arranged by size:
3765 337 112
Bpu10I CC'TnA_GC
Cuts at: 907 907
Size: 4214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 112 502 1390 2269 2761 3059 112
Size: 390 888 879 492 298 1267
Fragments arranged by size:
1267 888 879 492 390 298
BsaI GGTCTCn'nnnn_
Cuts at: 3918 3918
Size: 4214
BsaAI yAC'GTr
Cuts at: 1567 1567
Size: 4214
BsaBI GATnn'nnATC
Cuts at: 6 229 1728 3765 6
Size: 223 1499 2037 455
Fragments arranged by size:
2037 1499 455 223
BsaHI Gr'CG_yC
Cuts at: 500 875 1645 1893 2595 4091 500
Size: 375 770 248 702 1496 623
Fragments arranged by size:
1496 770 702 623 375 248
BsaWI w'CCGG_w
Cuts at: 1403 1924 2602 2876 3023 3434 1403
Size: 521 678 274 147 411 2183
Fragments arranged by size:
2183 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 301 331 745 775 301
Size: 30 414 30 3740
Fragments arranged by size:
3740 414 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 391
Size: 4214
BseYI C'CCAG_C
Cuts at: 1141 1639 2397 2974 3470 1141
Size: 498 758 577 496 1885
Fragments arranged by size:
1885 758 577 498 496
BsiEI CG_ry'CG
Cuts at: 104 240 1756 1802 3010 104
Size: 136 1516 46 1208 1308
Fragments arranged by size:
1516 1308 1208 136 46
BsiHKAI G_wGCw'C
Cuts at: 383 800 1228 2006 2196 2988 383
Size: 417 428 778 190 792 1609
Fragments arranged by size:
1609 792 778 428 417 190
BsmI GAATG_Cn'
Cuts at: 674 674
Size: 4214
BsmBI CGTCTCn'nnnn_
Cuts at: 1189 1189
Size: 4214
Bsp1286I G_dGCh'C
Cuts at: 172 383 429 800 1178 1206 1228 1839 1932
Size: 211 46 371 378 28 22 611 93
Cuts at: 1932 2006 2196 2258 2988 172
Size: 74 190 62 730 1398
Fragments arranged by size:
1398 730 611 378 371 211 190 93
74 62 46 28 22
BspEI T'CCGG_A
Cuts at: 3434 3434
Size: 4214
BspHI T'CATG_A
Cuts at: 3390 3390
Size: 4214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1264 1478 1780 2161 1264
Size: 214 302 381 3317
Fragments arranged by size:
3317 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2506 2560 4044 4209 50
Size: 2456 54 1484 165 55
Fragments arranged by size:
2456 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1109 2126 1109
Size: 1017 3197
BsrFI r'CCGG_y
Cuts at: 881 2212 2393 2602 881
Size: 1331 181 209 2493
Fragments arranged by size:
2493 1331 209 181
BssSI C'ACGA_G
Cuts at: 2485 2843 2485
Size: 358 3856
BstAPI GCAn_nnn'nTGC
Cuts at: 1591 1591
Size: 4214
BstBI TT'CG_AA
Cuts at: 2575 2575
Size: 4214
BstEII G'GTnAC_C
Cuts at: 638 638
Size: 4214
BstXI CCAn_nnnn'nTGG
Cuts at: 1041 1041
Size: 4214
BstYI r'GATC_y
Cuts at: 1 338 902 940 1127 1239 1706 1729 2064
Size: 337 564 38 187 112 467 23 335
Cuts at: 2064 2310 3322 3408 3420 3766 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 564 467 449 346 337 335 246
187 112 86 38 23 12
Bsu36I CC'TnA_GG
Cuts at: 1049 1049
Size: 4214
BtgI C'CryG_G
Cuts at: 107 107
Size: 4214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2164 2345 2508 2164
Size: 181 163 3870
Fragments arranged by size:
3870 181 163
BtsI GCAGTG_nn'
Cuts at: 312 912 1457 312
Size: 600 545 3069
Fragments arranged by size:
3069 600 545
DraI TTT'AAA
Cuts at: 1213 1213
Size: 4214
DrdI GACnn_nn'nnGTC
Cuts at: 121 499 1920 2778 121
Size: 378 1421 858 1557
Fragments arranged by size:
1557 1421 858 378
EaeI y'GGCC_r
Cuts at: 299 473 953 1799 1973 2364 2391 3736 299
Size: 174 480 846 174 391 27 1345 777
Fragments arranged by size:
1345 846 777 480 391 174 174 27
EagI C'GGCC_G
Cuts at: 1799 1799
Size: 4214
EarI CTCTTCn'nnn_
Cuts at: 2237 2447 2237
Size: 210 4004
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2422 2732 2878 4024 2422
Size: 310 146 1146 2612
Fragments arranged by size:
2612 1146 310 146
EcoICRI GAG'CTC
Cuts at: 381 1226 381
Size: 845 3369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 555 726 1063 1175 2039 2471 3218 3486
Size: 326 171 337 112 864 432 747 268
Cuts at: 3486 229
Size: 957
Fragments arranged by size:
957 864 747 432 337 326 268 171
112
EcoNI CCTnn'n_nnAGG
Cuts at: 433 541 433
Size: 108 4106
EcoO109I rG'GnC_Cy
Cuts at: 249 513 846 1174 1175 1353 249
Size: 264 333 328 1 178 3110
Fragments arranged by size:
3110 333 328 264 178 1
EcoRI G'AATT_C
Cuts at: 1218 1218
Size: 4214
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 4214
FspI TGC'GCA
Cuts at: 1995 1995
Size: 4214
HaeII r_GCGC'y
Cuts at: 207 588 1648 1896 2918 3897 207
Size: 381 1060 248 1022 979 524
Fragments arranged by size:
1060 1022 979 524 381 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1012 1044 2056 2086 2088 2118 1012
Size: 32 1012 30 2 30 3108
Fragments arranged by size:
3108 1012 32 30 30 2
HincII GTy'rAC
Cuts at: 807 879 1154 1253 807
Size: 72 275 99 3768
Fragments arranged by size:
3768 275 99 72
KasI G'GCGC_C
Cuts at: 1644 1892 1644
Size: 248 3966
KpnI G_GTAC'C
Cuts at: 1234 1234
Size: 4214
MluI A'CGCG_T
Cuts at: 3647 3647
Size: 4214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 590 788 1103 2885 3069 590
Size: 198 315 1782 184 1735
Fragments arranged by size:
1782 1735 315 198 184
MscI TGG'CCA
Cuts at: 301 475 955 1975 301
Size: 174 480 1020 2540
Fragments arranged by size:
2540 1020 480 174
MslI CAynn'nnrTG
Cuts at: 373 2203 2330 373
Size: 1830 127 2257
Fragments arranged by size:
2257 1830 127
MspA1I CmG'CkG
Cuts at: 1325 1639 1999 3012 3257 1325
Size: 314 360 1013 245 2282
Fragments arranged by size:
2282 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2395 2395
Size: 4214
NarI GG'CG_CC
Cuts at: 1645 1893 1645
Size: 248 3966
NcoI C'CATG_G
Cuts at: 107 107
Size: 4214
NgoMIV G'CCGG_C
Cuts at: 2393 2393
Size: 4214
NspI r_CATG'y
Cuts at: 864 1267 1767 2674 864
Size: 403 500 907 2404
Fragments arranged by size:
2404 907 500 403
PciI A'CATG_T
Cuts at: 2670 2670
Size: 4214
PflMI CCAn_nnn'nTGG
Cuts at: 495 495
Size: 4214
PmeI GTTT'AAAC
Cuts at: 1213 1213
Size: 4214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3353 3385 3353
Size: 32 4182
PpuMI rG'GwC_Cy
Cuts at: 513 846 513
Size: 333 3881
PshAI GACnn'nnGTC
Cuts at: 605 605
Size: 4214
PspOMI G'GGCC_C
Cuts at: 1174 1202 1174
Size: 28 4186
PspXI vC'TCGA_Gb
Cuts at: 403 403
Size: 4214
PstI C_TGCA'G
Cuts at: 671 869 1261 1946 671
Size: 198 392 685 2939
Fragments arranged by size:
2939 685 392 198
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4214
PvuII CAG'CTG
Cuts at: 1639 1999 1639
Size: 360 3854
RsrII CG'GwC_CG
Cuts at: 2409 2409
Size: 4214
SacI G_AGCT'C
Cuts at: 383 1228 383
Size: 845 3369
SalI G'TCGA_C
Cuts at: 1152 1251 1152
Size: 99 4115
SapI GCTCTTCn'nnn_
Cuts at: 2237 2447 2237
Size: 210 4004
SbfI CC_TGCA'GG
Cuts at: 1261 1261
Size: 4214
ScaI AGT'ACT
Cuts at: 484 3731 484
Size: 3247 967
SfcI C'TryA_G
Cuts at: 33 667 865 1257 1942 2935 3126 33
Size: 634 198 392 685 993 191 1121
Fragments arranged by size:
1121 993 685 634 392 198 191
SfoI GGC'GCC
Cuts at: 1646 1894 1646
Size: 248 3966
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4214
SmaI CCC'GGG
Cuts at: 283 421 982 1236 283
Size: 138 561 254 3261
Fragments arranged by size:
3261 561 254 138
SmlI C'TyrA_G
Cuts at: 127 403 517 1369 2284 2776 3038 127
Size: 276 114 852 915 492 262 1303
Fragments arranged by size:
1303 915 852 492 276 262 114
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4214
SphI G_CATG'C
Cuts at: 1267 1767 1267
Size: 500 3714
StyI C'CwwG_G
Cuts at: 107 470 689 711 929 1348 1702 107
Size: 363 219 22 218 419 354 2619
Fragments arranged by size:
2619 419 363 354 219 218 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1797 1930 2389 2598 1797
Size: 133 459 209 3413
Fragments arranged by size:
3413 459 209 133
TatI w'GTAC_w
Cuts at: 482 3729 482
Size: 3247 967
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 451 483 505 537 3731 3763 451
Size: 32 22 32 3194 32 902
Fragments arranged by size:
3194 902 32 32 32 22
Tth111I GACn'n_nGTC
Cuts at: 502 2011 502
Size: 1509 2705
XbaI T'CTAG_A
Cuts at: 1245 1245
Size: 4214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1709 1709
Size: 4214
XhoI C'TCGA_G
Cuts at: 403 403
Size: 4214
XmaI C'CCGG_G
Cuts at: 281 419 980 1234 281
Size: 138 561 254 3261
Fragments arranged by size:
3261 561 254 138
ZraI GAC'GTC
Cuts at: 501 876 501
Size: 375 3839
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AarI AflII AleI AlfI AloI AscI BaeI BbsI
BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI BsrGI
BssHII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PsiI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI