Restriction map for
pF1KE0945
(Circular) (Six-base) MAPSORT of: pF1KE0945.seq Check: 1123 from: 1 to: 4163
pF1KE0945 4163 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1201 1201
Size: 4163
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 4003 4003
Size: 4163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 379 753 1988 2420 3167 3435 270
Size: 109 374 1235 432 747 268 998
Fragments arranged by size:
1235 998 747 432 374 268 109
AflIII A'CryG_T
Cuts at: 504 626 1515 2619 3596 504
Size: 122 889 1104 977 1071
Fragments arranged by size:
1104 1071 977 889 122
AgeI A'CCGG_T
Cuts at: 2551 2551
Size: 4163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 437 437
Size: 4163
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 802 834 802
Size: 32 4131
AlwNI CAG_nnn'CTG
Cuts at: 483 753 1468 3035 483
Size: 270 715 1567 1611
Fragments arranged by size:
1611 1567 715 270
ApaLI G'TGCA_C
Cuts at: 2933 2933
Size: 4163
ApoI r'AATT_y
Cuts at: 1167 3516 3772 1167
Size: 2349 256 1558
Fragments arranged by size:
2349 1558 256
AseI AT'TA_AT
Cuts at: 20 674 20
Size: 654 3509
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 149 1183 149
Size: 1034 3129
BamHI G'GATC_C
Cuts at: 1188 1188
Size: 4163
BanI G'GyrC_C
Cuts at: 156 1179 1593 1841 1876 156
Size: 1023 414 248 35 2443
Fragments arranged by size:
2443 1023 414 248 35
BanII G_rGCy'C
Cuts at: 1177 2207 1177
Size: 1030 3133
BbeI G_GCGC'C
Cuts at: 1597 1845 1597
Size: 248 3915
BbsI GAAGACnn'nnnn_
Cuts at: 742 742
Size: 4163
BciVI GTATCCnnnnn_n'
Cuts at: 1107 2061 2822 4163 1107
Size: 954 761 1341 1107
Fragments arranged by size:
1341 1107 954 761
BclI T'GATC_A
Cuts at: 182 1683 182
Size: 1501 2662
BglI GCCn_nnn'nGGC
Cuts at: 698 698
Size: 4163
BglII A'GATC_T
Cuts at: 1678 3715 1678
Size: 2037 2126
BlpI GC'TnA_GC
Cuts at: 1275 1275
Size: 4163
Bme1580I G_kGCm'C
Cuts at: 703 992 1788 1881 2937 703
Size: 289 796 93 1056 1929
Fragments arranged by size:
1929 1056 796 289 93
BmgBI CAC'GTC
Cuts at: 903 903
Size: 4163
BmrI ACTGGGnnnn_n'
Cuts at: 741 1375 1456 1791 3935 741
Size: 634 81 335 2144 969
Fragments arranged by size:
2144 969 634 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1073 2530 1073
Size: 1457 2706
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 664 857 957 1111 466
Size: 198 193 100 154 3518
Fragments arranged by size:
3518 198 193 154 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 597 1339 2218 2710 3008 597
Size: 742 879 492 298 1752
Fragments arranged by size:
1752 879 742 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1087 3867 1087
Size: 2780 1383
BsaAI yAC'GTr
Cuts at: 629 1516 629
Size: 887 3276
BsaBI GATnn'nnATC
Cuts at: 6 1677 3714 6
Size: 1671 2037 455
Fragments arranged by size:
2037 1671 455
BsaHI Gr'CG_yC
Cuts at: 794 1594 1842 2544 4040 794
Size: 800 248 702 1496 917
Fragments arranged by size:
1496 917 800 702 248
BsaWI w'CCGG_w
Cuts at: 1352 1873 2551 2825 2972 3383 1352
Size: 521 678 274 147 411 2132
Fragments arranged by size:
2132 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 802 832 887 917 802
Size: 30 55 30 4048
Fragments arranged by size:
4048 55 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 230 346 230
Size: 116 4047
BseYI C'CCAG_C
Cuts at: 1588 2346 2923 3419 1588
Size: 758 577 496 2332
Fragments arranged by size:
2332 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 667
Size: 4163
BsiEI CG_ry'CG
Cuts at: 104 1705 1751 2959 104
Size: 1601 46 1208 1308
Fragments arranged by size:
1601 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 416 1177 1955 2145 2937 416
Size: 761 778 190 792 1642
Fragments arranged by size:
1642 792 778 761 190
Bsp1286I G_dGCh'C
Cuts at: 416 703 992 1177 1788 1881 1955 2145 2207
Size: 287 289 185 611 93 74 190 62
Cuts at: 2207 2937 416
Size: 730 1642
Fragments arranged by size:
1642 730 611 289 287 190 185 93
74 62
BspEI T'CCGG_A
Cuts at: 3383 3383
Size: 4163
BspHI T'CATG_A
Cuts at: 3339 3339
Size: 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 689 737 1213 1427 1729 2110 689
Size: 48 476 214 302 381 2742
Fragments arranged by size:
2742 476 381 302 214 48
BsrBI CCG'CTC
Cuts at: 50 2455 2509 3993 4158 50
Size: 2405 54 1484 165 55
Fragments arranged by size:
2405 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2075 2075
Size: 4163
BsrFI r'CCGG_y
Cuts at: 2161 2342 2551 2161
Size: 181 209 3773
Fragments arranged by size:
3773 209 181
BssSI C'ACGA_G
Cuts at: 2434 2792 2434
Size: 358 3805
BstAPI GCAn_nnn'nTGC
Cuts at: 1540 1540
Size: 4163
BstBI TT'CG_AA
Cuts at: 2524 2524
Size: 4163
BstYI r'GATC_y
Cuts at: 1 959 1188 1655 1678 2013 2259 3271 3357
Size: 958 229 467 23 335 246 1012 86
Cuts at: 3357 3369 3715 1
Size: 12 346 449
Fragments arranged by size:
1012 958 467 449 346 335 246 229
86 23 12
BtgI C'CryG_G
Cuts at: 107 468 653 107
Size: 361 185 3617
Fragments arranged by size:
3617 361 185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 219 2113 2294 2457 219
Size: 1894 181 163 1925
Fragments arranged by size:
1925 1894 181 163
BtsI GCAGTG_nn'
Cuts at: 1073 1406 1073
Size: 333 3830
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 1869 2727 1869
Size: 858 3305
EaeI y'GGCC_r
Cuts at: 650 840 1748 1922 2313 2340 3685 650
Size: 190 908 174 391 27 1345 1128
Fragments arranged by size:
1345 1128 908 391 190 174 27
EagI C'GGCC_G
Cuts at: 1748 1748
Size: 4163
EarI CTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2371 2681 2827 3973 2371
Size: 310 146 1146 2561
Fragments arranged by size:
2561 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1175 1175
Size: 4163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 379 466 664 753 857 957 1111 1988
Size: 109 87 198 89 104 100 154 877
Cuts at: 1988 2420 3167 3435 270
Size: 432 747 268 998
Fragments arranged by size:
998 877 747 432 268 198 154 109
104 100 89 87
EcoO109I rG'GnC_Cy
Cuts at: 1302 1302
Size: 4163
EcoRI G'AATT_C
Cuts at: 1167 1167
Size: 4163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 408 2182 408
Size: 1774 2389
FspI TGC'GCA
Cuts at: 1944 1944
Size: 4163
HaeII r_GCGC'y
Cuts at: 1597 1845 2867 3846 1597
Size: 248 1022 979 1914
Fragments arranged by size:
1914 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 186 218 2005 2035 2037 2067 186
Size: 32 1787 30 2 30 2282
Fragments arranged by size:
2282 1787 32 30 30 2
HincII GTy'rAC
Cuts at: 1202 1202
Size: 4163
KasI G'GCGC_C
Cuts at: 1593 1841 1593
Size: 248 3915
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 746 2834 3018 746
Size: 2088 184 1891
Fragments arranged by size:
2088 1891 184
MscI TGG'CCA
Cuts at: 652 1924 652
Size: 1272 2891
MslI CAynn'nnrTG
Cuts at: 2152 2279 2152
Size: 127 4036
MspA1I CmG'CkG
Cuts at: 166 357 1274 1588 1948 2961 3206 166
Size: 191 917 314 360 1013 245 1123
Fragments arranged by size:
1123 1013 917 360 314 245 191
NaeI GCC'GGC
Cuts at: 2344 2344
Size: 4163
NarI GG'CG_CC
Cuts at: 1594 1842 1594
Size: 248 3915
NcoI C'CATG_G
Cuts at: 107 468 653 107
Size: 361 185 3617
Fragments arranged by size:
3617 361 185
NdeI CA'TA_TG
Cuts at: 933 933
Size: 4163
NgoMIV G'CCGG_C
Cuts at: 2342 2342
Size: 4163
NspI r_CATG'y
Cuts at: 508 1046 1216 1716 2623 508
Size: 538 170 500 907 2048
Fragments arranged by size:
2048 907 538 500 170
PciI A'CATG_T
Cuts at: 504 2619 504
Size: 2115 2048
PflMI CCAn_nnn'nTGG
Cuts at: 169 169
Size: 4163
PfoI T'CCnGG_A
Cuts at: 820 820
Size: 4163
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PmlI CAC'GTG
Cuts at: 629 629
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 802 834 3302 3334 802
Size: 32 2468 32 1631
Fragments arranged by size:
2468 1631 32 32
PstI C_TGCA'G
Cuts at: 479 1210 1895 479
Size: 731 685 2747
Fragments arranged by size:
2747 731 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 166 357 1588 1948 166
Size: 191 1231 360 2381
Fragments arranged by size:
2381 1231 360 191
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 1177 1177
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SfcI C'TryA_G
Cuts at: 33 475 1206 1891 2884 3075 33
Size: 442 731 685 993 191 1121
Fragments arranged by size:
1121 993 731 685 442 191
SfoI GGC'GCC
Cuts at: 1595 1843 1595
Size: 248 3915
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SmaI CCC'GGG
Cuts at: 1185 1185
Size: 4163
SmlI C'TyrA_G
Cuts at: 576 1318 2233 2725 2987 576
Size: 742 915 492 262 1752
Fragments arranged by size:
1752 915 742 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 1046 1216 1716 1046
Size: 170 500 3493
Fragments arranged by size:
3493 500 170
StuI AGG'CCT
Cuts at: 140 140
Size: 4163
StyI C'CwwG_G
Cuts at: 107 316 468 495 653 1297 1651 107
Size: 209 152 27 158 644 354 2619
Fragments arranged by size:
2619 644 354 209 158 152 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1746 1879 2338 2547 1746
Size: 133 459 209 3362
Fragments arranged by size:
3362 459 209 133
TatI w'GTAC_w
Cuts at: 3678 3678
Size: 4163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 255 287 887 919 3680 3712 255
Size: 32 600 32 2761 32 706
Fragments arranged by size:
2761 706 600 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 661 741 1960 661
Size: 80 1219 2864
Fragments arranged by size:
2864 1219 80
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 881 981 1658 881
Size: 100 677 3386
Fragments arranged by size:
3386 677 100
XmaI C'CCGG_G
Cuts at: 1183 1183
Size: 4163
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PfoI PmeI PmlI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI