Restriction map for
pF1KE0944
(Circular) (Six-base) MAPSORT of: pF1KE0944.seq Check: 9351 from: 1 to: 4142
pF1KE0944 4142 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 544 544
Size: 4142
AatII G_ACGT'C
Cuts at: 1103 1103
Size: 4142
AccI GT'mk_AC
Cuts at: 124 1180 124
Size: 1056 3086
Acc65I G'GTAC_C
Cuts at: 860 1158 860
Size: 298 3844
AclI AA'CG_TT
Cuts at: 1136 3982 1136
Size: 2846 1296
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 879 1967 2399 3146 3414 879
Size: 1088 432 747 268 1607
Fragments arranged by size:
1607 1088 747 432 268
AflIII A'CryG_T
Cuts at: 815 1494 2598 3575 815
Size: 679 1104 977 1382
Fragments arranged by size:
1382 1104 977 679
AgeI A'CCGG_T
Cuts at: 2530 2530
Size: 4142
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 223 255 223
Size: 32 4110
AlwNI CAG_nnn'CTG
Cuts at: 1447 3014 1447
Size: 1567 2575
ApaLI G'TGCA_C
Cuts at: 2912 2912
Size: 4142
ApoI r'AATT_y
Cuts at: 646 1010 1053 1146 3495 3751 646
Size: 364 43 93 2349 256 1037
Fragments arranged by size:
2349 1037 364 256 93 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 4142
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
AvaI C'yCGr_G
Cuts at: 243 253 674 798 1162 243
Size: 10 421 124 364 3223
Fragments arranged by size:
3223 421 364 124 10
BamHI G'GATC_C
Cuts at: 1167 1167
Size: 4142
BanI G'GyrC_C
Cuts at: 411 860 1092 1158 1572 1820 1855 411
Size: 449 232 66 414 248 35 2698
Fragments arranged by size:
2698 449 414 248 232 66 35
BanII G_rGCy'C
Cuts at: 212 250 986 1156 2186 212
Size: 38 736 170 1030 2168
Fragments arranged by size:
2168 1030 736 170 38
BbeI G_GCGC'C
Cuts at: 1576 1824 1576
Size: 248 3894
BbsI GAAGACnn'nnnn_
Cuts at: 704 704
Size: 4142
BciVI GTATCCnnnnn_n'
Cuts at: 2040 2801 4142 2040
Size: 761 1341 2040
Fragments arranged by size:
2040 1341 761
BclI T'GATC_A
Cuts at: 1662 1662
Size: 4142
BfrBI ATG'CAT
Cuts at: 519 519
Size: 4142
BglII A'GATC_T
Cuts at: 773 1657 3694 773
Size: 884 2037 1221
Fragments arranged by size:
2037 1221 884
BlpI GC'TnA_GC
Cuts at: 1254 1254
Size: 4142
Bme1580I G_kGCm'C
Cuts at: 1767 1860 2916 1767
Size: 93 1056 2993
Fragments arranged by size:
2993 1056 93
BmgBI CAC'GTC
Cuts at: 816 816
Size: 4142
BmrI ACTGGGnnnn_n'
Cuts at: 1354 1435 1770 3914 1354
Size: 81 335 2144 1582
Fragments arranged by size:
2144 1582 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2509 2509
Size: 4142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 337
Size: 4142
Bpu10I CC'TnA_GC
Cuts at: 1070 1070
Size: 4142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 1318 2197 2689 2987 619
Size: 699 879 492 298 1774
Fragments arranged by size:
1774 879 699 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 317 469 3846 317
Size: 152 3377 613
Fragments arranged by size:
3377 613 152
BsaAI yAC'GTr
Cuts at: 762 1495 762
Size: 733 3409
BsaBI GATnn'nnATC
Cuts at: 6 1656 3693 6
Size: 1650 2037 455
Fragments arranged by size:
2037 1650 455
BsaHI Gr'CG_yC
Cuts at: 701 1100 1573 1821 2523 4019 701
Size: 399 473 248 702 1496 824
Fragments arranged by size:
1496 824 702 473 399 248
BsaWI w'CCGG_w
Cuts at: 1331 1852 2530 2804 2951 3362 1331
Size: 521 678 274 147 411 2111
Fragments arranged by size:
2111 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 223 253 314 344 355 385 223
Size: 30 61 30 11 30 3980
Fragments arranged by size:
3980 61 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 376 568 571 376
Size: 192 3 3947
Fragments arranged by size:
3947 192 3
BseYI C'CCAG_C
Cuts at: 211 883 1567 2325 2902 3398 211
Size: 672 684 758 577 496 955
Fragments arranged by size:
955 758 684 672 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 334
Size: 4142
BsiEI CG_ry'CG
Cuts at: 104 1684 1730 2938 104
Size: 1580 46 1208 1308
Fragments arranged by size:
1580 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1156 1934 2124 2916 1156
Size: 778 190 792 2382
Fragments arranged by size:
2382 792 778 190
BsmI GAATG_Cn'
Cuts at: 519 968 519
Size: 449 3693
BsmBI CGTCTCn'nnnn_
Cuts at: 1053 1053
Size: 4142
Bsp1286I G_dGCh'C
Cuts at: 212 250 986 1156 1767 1860 1934 2124 2186
Size: 38 736 170 611 93 74 190 62
Cuts at: 2186 2916 212
Size: 730 1438
Fragments arranged by size:
1438 736 730 611 190 170 93 74
62 38
BspEI T'CCGG_A
Cuts at: 3362 3362
Size: 4142
BspHI T'CATG_A
Cuts at: 3318 3318
Size: 4142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 544 1192 1406 1708 2089 544
Size: 648 214 302 381 2597
Fragments arranged by size:
2597 648 381 302 214
BsrBI CCG'CTC
Cuts at: 50 993 2434 2488 3972 4137 50
Size: 943 1441 54 1484 165 55
Fragments arranged by size:
1484 1441 943 165 55 54
BsrDI GCAATG_nn'
Cuts at: 273 2054 273
Size: 1781 2361
BsrFI r'CCGG_y
Cuts at: 2140 2321 2530 2140
Size: 181 209 3752
Fragments arranged by size:
3752 209 181
BsrGI T'GTAC_A
Cuts at: 839 839
Size: 4142
BssSI C'ACGA_G
Cuts at: 2413 2771 2413
Size: 358 3784
BstAPI GCAn_nnn'nTGC
Cuts at: 1066 1519 1066
Size: 453 3689
BstBI TT'CG_AA
Cuts at: 179 2503 179
Size: 2324 1818
BstXI CCAn_nnnn'nTGG
Cuts at: 852 852
Size: 4142
BstYI r'GATC_y
Cuts at: 1 773 902 1167 1634 1657 1992 2238 3250
Size: 772 129 265 467 23 335 246 1012
Cuts at: 3250 3336 3348 3694 1
Size: 86 12 346 449
Fragments arranged by size:
1012 772 467 449 346 335 265 246
129 86 23 12
BtgI C'CryG_G
Cuts at: 203 203
Size: 4142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2092 2273 2436 2092
Size: 181 163 3798
Fragments arranged by size:
3798 181 163
BtsI GCAGTG_nn'
Cuts at: 599 600 1385 599
Size: 1 785 3356
Fragments arranged by size:
3356 785 1
DraI TTT'AAA
Cuts at: 1141 1141
Size: 4142
DrdI GACnn_nn'nnGTC
Cuts at: 1848 2706 1848
Size: 858 3284
EaeI y'GGCC_r
Cuts at: 912 1727 1901 2292 2319 3664 912
Size: 815 174 391 27 1345 1390
Fragments arranged by size:
1390 1345 815 391 174 27
EagI C'GGCC_G
Cuts at: 1727 1727
Size: 4142
EarI CTCTTCn'nnn_
Cuts at: 176 659 898 1106 2165 2375 176
Size: 483 239 208 1059 210 1943
Fragments arranged by size:
1943 1059 483 239 210 208
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2350 2660 2806 3952 2350
Size: 310 146 1146 2540
Fragments arranged by size:
2540 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1154 1154
Size: 4142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 879 1967 2399 3146 3414 337
Size: 542 1088 432 747 268 1065
Fragments arranged by size:
1088 1065 747 542 432 268
EcoO109I rG'GnC_Cy
Cuts at: 630 1281 630
Size: 651 3491
EcoRI G'AATT_C
Cuts at: 1053 1146 1053
Size: 93 4049
EcoRV GAT'ATC
Cuts at: 387 387
Size: 4142
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2161 2161
Size: 4142
FspI TGC'GCA
Cuts at: 1923 1923
Size: 4142
HaeII r_GCGC'y
Cuts at: 1576 1824 2846 3825 1576
Size: 248 1022 979 1893
Fragments arranged by size:
1893 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 175 207 718 750 922 954 1984 2014 2016
Size: 32 511 32 172 32 1030 30 2
Cuts at: 2016 2046 175
Size: 30 2271
Fragments arranged by size:
2271 1030 511 172 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1104 1181 1104
Size: 77 4065
KasI G'GCGC_C
Cuts at: 1572 1820 1572
Size: 248 3894
KpnI G_GTAC'C
Cuts at: 864 1162 864
Size: 298 3844
MluI A'CGCG_T
Cuts at: 3575 3575
Size: 4142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 287 761 2813 2997 287
Size: 474 2052 184 1432
Fragments arranged by size:
2052 1432 474 184
MscI TGG'CCA
Cuts at: 914 1903 914
Size: 989 3153
MslI CAynn'nnrTG
Cuts at: 605 850 2131 2258 605
Size: 245 1281 127 2489
Fragments arranged by size:
2489 1281 245 127
MspA1I CmG'CkG
Cuts at: 205 356 392 1089 1253 1567 1927 2940 3185
Size: 151 36 697 164 314 360 1013 245
Cuts at: 3185 205
Size: 1162
Fragments arranged by size:
1162 1013 697 360 314 245 164 151
36
NaeI GCC'GGC
Cuts at: 2323 2323
Size: 4142
NarI GG'CG_CC
Cuts at: 1573 1821 1573
Size: 248 3894
NdeI CA'TA_TG
Cuts at: 1062 1062
Size: 4142
NgoMIV G'CCGG_C
Cuts at: 2321 2321
Size: 4142
NsiI A_TGCA'T
Cuts at: 521 521
Size: 4142
NspI r_CATG'y
Cuts at: 519 1195 1695 2602 519
Size: 676 500 907 2059
Fragments arranged by size:
2059 907 676 500
PciI A'CATG_T
Cuts at: 2598 2598
Size: 4142
PmeI GTTT'AAAC
Cuts at: 1141 1141
Size: 4142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 223 255 3281 3313 223
Size: 32 3026 32 1052
Fragments arranged by size:
3026 1052 32 32
PpuMI rG'GwC_Cy
Cuts at: 630 630
Size: 4142
PshAI GACnn'nnGTC
Cuts at: 676 676
Size: 4142
PstI C_TGCA'G
Cuts at: 1189 1874 1189
Size: 685 3457
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4142
PvuII CAG'CTG
Cuts at: 392 1089 1567 1927 392
Size: 697 478 360 2607
Fragments arranged by size:
2607 697 478 360
RsrII CG'GwC_CG
Cuts at: 2337 2337
Size: 4142
SacI G_AGCT'C
Cuts at: 1156 1156
Size: 4142
SacII CC_GC'GG
Cuts at: 206 206
Size: 4142
SalI G'TCGA_C
Cuts at: 1179 1179
Size: 4142
SapI GCTCTTCn'nnn_
Cuts at: 2165 2375 2165
Size: 210 3932
SbfI CC_TGCA'GG
Cuts at: 1189 1189
Size: 4142
ScaI AGT'ACT
Cuts at: 3659 3659
Size: 4142
SfcI C'TryA_G
Cuts at: 33 1034 1185 1870 2863 3054 33
Size: 1001 151 685 993 191 1121
Fragments arranged by size:
1121 1001 993 685 191 151
SfoI GGC'GCC
Cuts at: 1574 1822 1574
Size: 248 3894
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
SmaI CCC'GGG
Cuts at: 1164 1164
Size: 4142
SmlI C'TyrA_G
Cuts at: 634 1297 2212 2704 2966 634
Size: 663 915 492 262 1810
Fragments arranged by size:
1810 915 663 492 262
SnaBI TAC'GTA
Cuts at: 762 762
Size: 4142
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4142
SphI G_CATG'C
Cuts at: 1195 1695 1195
Size: 500 3642
StyI C'CwwG_G
Cuts at: 944 1024 1276 1630 944
Size: 80 252 354 3456
Fragments arranged by size:
3456 354 252 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 460 1725 1858 2317 2526 460
Size: 1265 133 459 209 2076
Fragments arranged by size:
2076 1265 459 209 133
TatI w'GTAC_w
Cuts at: 839 3657 839
Size: 2818 1324
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 355 387 3659 3691 355
Size: 32 3272 32 806
Fragments arranged by size:
3272 806 32 32
Tth111I GACn'n_nGTC
Cuts at: 936 1939 936
Size: 1003 3139
XbaI T'CTAG_A
Cuts at: 1173 1173
Size: 4142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1637 1637
Size: 4142
XmaI C'CCGG_G
Cuts at: 1162 1162
Size: 4142
ZraI GAC'GTC
Cuts at: 1101 1101
Size: 4142
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NsiI NspI PciI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmtI BsiWI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NcoI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI
XhoI XmnI