Restriction map for
pF1KE0943
(Circular) (Six-base) MAPSORT of: pF1KE0943.seq Check: 4736 from: 1 to: 4121
pF1KE0943 4121 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 290 653 858 921 290
Size: 363 205 63 3490
Fragments arranged by size:
3490 363 205 63
AatII G_ACGT'C
Cuts at: 1043 1043
Size: 4121
AccI GT'mk_AC
Cuts at: 1159 1159
Size: 4121
Acc65I G'GTAC_C
Cuts at: 1137 1137
Size: 4121
AclI AA'CG_TT
Cuts at: 3961 3961
Size: 4121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 924 1946 2378 3125 3393 924
Size: 1022 432 747 268 1652
Fragments arranged by size:
1652 1022 747 432 268
AflIII A'CryG_T
Cuts at: 1473 2577 3554 1473
Size: 1104 977 2040
Fragments arranged by size:
2040 1104 977
AgeI A'CCGG_T
Cuts at: 2509 2509
Size: 4121
AleI CACnn'nnGTG
Cuts at: 841 841
Size: 4121
AlwNI CAG_nnn'CTG
Cuts at: 411 487 1426 2993 411
Size: 76 939 1567 1539
Fragments arranged by size:
1567 1539 939 76
ApaI G_GGCC'C
Cuts at: 602 720 602
Size: 118 4003
ApaLI G'TGCA_C
Cuts at: 235 2891 235
Size: 2656 1465
ApoI r'AATT_y
Cuts at: 1125 3474 3730 1125
Size: 2349 256 1516
Fragments arranged by size:
2349 1516 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4121
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
AvaI C'yCGr_G
Cuts at: 459 601 1141 459
Size: 142 540 3439
Fragments arranged by size:
3439 540 142
BamHI G'GATC_C
Cuts at: 1146 1146
Size: 4121
BanI G'GyrC_C
Cuts at: 127 791 1021 1137 1551 1799 1834 127
Size: 664 230 116 414 248 35 2414
Fragments arranged by size:
2414 664 414 248 230 116 35
BanII G_rGCy'C
Cuts at: 517 602 720 1135 2165 517
Size: 85 118 415 1030 2473
Fragments arranged by size:
2473 1030 415 118 85
BbeI G_GCGC'C
Cuts at: 1555 1803 1555
Size: 248 3873
BciVI GTATCCnnnnn_n'
Cuts at: 2019 2780 4121 2019
Size: 761 1341 2019
Fragments arranged by size:
2019 1341 761
BclI T'GATC_A
Cuts at: 1641 1641
Size: 4121
BglI GCCn_nnn'nGGC
Cuts at: 548 548
Size: 4121
BglII A'GATC_T
Cuts at: 1636 3673 1636
Size: 2037 2084
BlpI GC'TnA_GC
Cuts at: 1233 1233
Size: 4121
Bme1580I G_kGCm'C
Cuts at: 239 602 720 1746 1839 2895 239
Size: 363 118 1026 93 1056 1465
Fragments arranged by size:
1465 1056 1026 363 118 93
BmrI ACTGGGnnnn_n'
Cuts at: 414 1333 1414 1749 3893 414
Size: 919 81 335 2144 642
Fragments arranged by size:
2144 919 642 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 4121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 302
Size: 4121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1297 2176 2668 2966 1297
Size: 879 492 298 2452
Fragments arranged by size:
2452 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3825 3825
Size: 4121
BsaAI yAC'GTr
Cuts at: 1474 1474
Size: 4121
BsaBI GATnn'nnATC
Cuts at: 6 1635 3672 6
Size: 1629 2037 455
Fragments arranged by size:
2037 1629 455
BsaHI Gr'CG_yC
Cuts at: 1040 1552 1800 2502 3998 1040
Size: 512 248 702 1496 1163
Fragments arranged by size:
1496 1163 702 512 248
BsaWI w'CCGG_w
Cuts at: 392 1072 1310 1831 2509 2783 2930 3341 392
Size: 680 238 521 678 274 147 411 1172
Fragments arranged by size:
1172 680 678 521 411 274 238 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 475 505 997 1027 475
Size: 30 492 30 3569
Fragments arranged by size:
3569 492 30 30
BseYI C'CCAG_C
Cuts at: 268 1546 2304 2881 3377 268
Size: 1278 758 577 496 1012
Fragments arranged by size:
1278 1012 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 949
Size: 4121
BsiEI CG_ry'CG
Cuts at: 104 260 1663 1709 2917 104
Size: 156 1403 46 1208 1308
Fragments arranged by size:
1403 1308 1208 156 46
BsiHKAI G_wGCw'C
Cuts at: 239 517 896 1135 1913 2103 2895 239
Size: 278 379 239 778 190 792 1465
Fragments arranged by size:
1465 792 778 379 278 239 190
Bsp1286I G_dGCh'C
Cuts at: 239 517 602 720 896 1135 1746 1839 1913
Size: 278 85 118 176 239 611 93 74
Cuts at: 1913 2103 2165 2895 239
Size: 190 62 730 1465
Fragments arranged by size:
1465 730 611 278 239 190 176 118
93 85 74 62
BspEI T'CCGG_A
Cuts at: 392 3341 392
Size: 2949 1172
BspHI T'CATG_A
Cuts at: 3297 3297
Size: 4121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 290 653 858 921 1171 1385 1687 2068 290
Size: 363 205 63 250 214 302 381 2343
Fragments arranged by size:
2343 381 363 302 250 214 205 63
BsrBI CCG'CTC
Cuts at: 50 202 264 2413 2467 3951 4116 50
Size: 152 62 2149 54 1484 165 55
Fragments arranged by size:
2149 1484 165 152 62 55 54
BsrDI GCAATG_nn'
Cuts at: 924 2033 924
Size: 1109 3012
BsrFI r'CCGG_y
Cuts at: 323 330 425 611 2119 2300 2509 323
Size: 7 95 186 1508 181 209 1935
Fragments arranged by size:
1935 1508 209 186 181 95 7
BsrGI T'GTAC_A
Cuts at: 110 110
Size: 4121
BssHII G'CGCG_C
Cuts at: 220 220
Size: 4121
BssSI C'ACGA_G
Cuts at: 2392 2750 2392
Size: 358 3763
BstAPI GCAn_nnn'nTGC
Cuts at: 900 1498 900
Size: 598 3523
BstBI TT'CG_AA
Cuts at: 2482 2482
Size: 4121
BstXI CCAn_nnnn'nTGG
Cuts at: 561 729 561
Size: 168 3953
BstYI r'GATC_y
Cuts at: 1 386 1146 1613 1636 1971 2217 3229 3315
Size: 385 760 467 23 335 246 1012 86
Cuts at: 3315 3327 3673 1
Size: 12 346 449
Fragments arranged by size:
1012 760 467 449 385 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 494 1053 494
Size: 559 3562
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2071 2252 2415 2071
Size: 181 163 3777
Fragments arranged by size:
3777 181 163
BtsI GCAGTG_nn'
Cuts at: 1364 1364
Size: 4121
DraI TTT'AAA
Cuts at: 1120 1120
Size: 4121
DraIII CAC_nnn'GTG
Cuts at: 422 422
Size: 4121
DrdI GACnn_nn'nnGTC
Cuts at: 1827 2685 1827
Size: 858 3263
EaeI y'GGCC_r
Cuts at: 257 427 593 616 674 1706 1880 2271 2298
Size: 170 166 23 58 1032 174 391 27
Cuts at: 2298 3643 257
Size: 1345 735
Fragments arranged by size:
1345 1032 735 391 174 170 166 58
27 23
EagI C'GGCC_G
Cuts at: 257 1706 257
Size: 1449 2672
EarI CTCTTCn'nnn_
Cuts at: 2144 2354 2144
Size: 210 3911
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2329 2639 2785 3931 2329
Size: 310 146 1146 2519
Fragments arranged by size:
2519 1146 310 146
EcoICRI GAG'CTC
Cuts at: 515 1133 515
Size: 618 3503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 924 1946 2378 3125 3393 302
Size: 622 1022 432 747 268 1030
Fragments arranged by size:
1030 1022 747 622 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 286 286
Size: 4121
EcoO109I rG'GnC_Cy
Cuts at: 550 575 667 684 717 827 1013 1260 550
Size: 25 92 17 33 110 186 247 3411
Fragments arranged by size:
3411 247 186 110 92 33 25 17
EcoRI G'AATT_C
Cuts at: 1125 1125
Size: 4121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 4121
FspI TGC'GCA
Cuts at: 1902 1902
Size: 4121
HaeII r_GCGC'y
Cuts at: 1555 1803 2825 3804 1555
Size: 248 1022 979 1872
Fragments arranged by size:
1872 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 475 507 848 880 997 1029 1963 1993 1995
Size: 32 341 32 117 32 934 30 2
Cuts at: 1995 2025 475
Size: 30 2571
Fragments arranged by size:
2571 934 341 117 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1160 1160
Size: 4121
KasI G'GCGC_C
Cuts at: 1551 1799 1551
Size: 248 3873
KpnI G_GTAC'C
Cuts at: 1141 1141
Size: 4121
MluI A'CGCG_T
Cuts at: 3554 3554
Size: 4121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 137 2792 2976 137
Size: 2655 184 1282
Fragments arranged by size:
2655 1282 184
MscI TGG'CCA
Cuts at: 1882 1882
Size: 4121
MslI CAynn'nnrTG
Cuts at: 797 841 2110 2237 797
Size: 44 1269 127 2681
Fragments arranged by size:
2681 1269 127 44
MspA1I CmG'CkG
Cuts at: 408 651 1232 1546 1906 2919 3164 408
Size: 243 581 314 360 1013 245 1365
Fragments arranged by size:
1365 1013 581 360 314 245 243
NaeI GCC'GGC
Cuts at: 332 427 613 2302 332
Size: 95 186 1689 2151
Fragments arranged by size:
2151 1689 186 95
NarI GG'CG_CC
Cuts at: 1552 1800 1552
Size: 248 3873
NcoI C'CATG_G
Cuts at: 494 494
Size: 4121
NgoMIV G'CCGG_C
Cuts at: 330 425 611 2300 330
Size: 95 186 1689 2151
Fragments arranged by size:
2151 1689 186 95
NspI r_CATG'y
Cuts at: 1104 1174 1674 2581 1104
Size: 70 500 907 2644
Fragments arranged by size:
2644 907 500 70
PasI CC'CwG_GG
Cuts at: 690 690
Size: 4121
PciI A'CATG_T
Cuts at: 2577 2577
Size: 4121
PflMI CCAn_nnn'nTGG
Cuts at: 500 713 768 853 500
Size: 213 55 85 3768
Fragments arranged by size:
3768 213 85 55
PfoI T'CCnGG_A
Cuts at: 465 465
Size: 4121
PmeI GTTT'AAAC
Cuts at: 1120 1120
Size: 4121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3260 3292 3260
Size: 32 4089
PpuMI rG'GwC_Cy
Cuts at: 575 684 827 1013 575
Size: 109 143 186 3683
Fragments arranged by size:
3683 186 143 109
PspOMI G'GGCC_C
Cuts at: 598 716 598
Size: 118 4003
PstI C_TGCA'G
Cuts at: 290 584 650 1109 1168 1853 290
Size: 294 66 459 59 685 2558
Fragments arranged by size:
2558 685 459 294 66 59
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4121
PvuII CAG'CTG
Cuts at: 651 1546 1906 651
Size: 895 360 2866
Fragments arranged by size:
2866 895 360
RsrII CG'GwC_CG
Cuts at: 2316 2316
Size: 4121
SacI G_AGCT'C
Cuts at: 517 1135 517
Size: 618 3503
SalI G'TCGA_C
Cuts at: 1158 1158
Size: 4121
SanDI GG'GwC_CC
Cuts at: 575 827 1013 575
Size: 252 186 3683
Fragments arranged by size:
3683 252 186
SapI GCTCTTCn'nnn_
Cuts at: 2144 2354 2144
Size: 210 3911
SbfI CC_TGCA'GG
Cuts at: 1168 1168
Size: 4121
ScaI AGT'ACT
Cuts at: 3638 3638
Size: 4121
SexAI A'CCwGG_T
Cuts at: 560 560
Size: 4121
SfcI C'TryA_G
Cuts at: 33 286 580 646 1105 1164 1849 2842 3033
Size: 253 294 66 459 59 685 993 191
Cuts at: 3033 33
Size: 1121
Fragments arranged by size:
1121 993 685 459 294 253 191 66
59
SfoI GGC'GCC
Cuts at: 1553 1801 1553
Size: 248 3873
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
SmaI CCC'GGG
Cuts at: 1143 1143
Size: 4121
SmlI C'TyrA_G
Cuts at: 1276 2191 2683 2945 1276
Size: 915 492 262 2452
Fragments arranged by size:
2452 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4121
SphI G_CATG'C
Cuts at: 1104 1174 1674 1104
Size: 70 500 3551
Fragments arranged by size:
3551 500 70
StuI AGG'CCT
Cuts at: 457 457
Size: 4121
StyI C'CwwG_G
Cuts at: 494 1255 1609 494
Size: 761 354 3006
Fragments arranged by size:
3006 761 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 264 433 717 809 1704 1837 2296 2505 264
Size: 169 284 92 895 133 459 209 1880
Fragments arranged by size:
1880 895 459 284 209 169 133 92
TatI w'GTAC_w
Cuts at: 110 3636 110
Size: 3526 595
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4089
Tth111I GACn'n_nGTC
Cuts at: 1918 1918
Size: 4121
XbaI T'CTAG_A
Cuts at: 1152 1152
Size: 4121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1616 1616
Size: 4121
XmaI C'CCGG_G
Cuts at: 1141 1141
Size: 4121
ZraI GAC'GTC
Cuts at: 1041 1041
Size: 4121
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BseRI
BsiWI BsmI BsmBI BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SacII SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI