Restriction map for
pF1KE0942
(Circular) (Six-base) MAPSORT of: pF1KE0942.seq Check: 2873 from: 1 to: 3995
pF1KE0942 3995 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1033 1033
Size: 3995
Acc65I G'GTAC_C
Cuts at: 1011 1011
Size: 3995
AclI AA'CG_TT
Cuts at: 3835 3835
Size: 3995
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1820 2252 2999 3267 1820
Size: 432 747 268 2548
Fragments arranged by size:
2548 747 432 268
AflIII A'CryG_T
Cuts at: 408 1347 2451 3428 408
Size: 939 1104 977 975
Fragments arranged by size:
1104 977 975 939
AgeI A'CCGG_T
Cuts at: 2383 2383
Size: 3995
AleI CACnn'nnGTG
Cuts at: 973 973
Size: 3995
AlwNI CAG_nnn'CTG
Cuts at: 243 702 1300 2867 243
Size: 459 598 1567 1371
Fragments arranged by size:
1567 1371 598 459
ApaLI G'TGCA_C
Cuts at: 2765 2765
Size: 3995
ApoI r'AATT_y
Cuts at: 999 3348 3604 999
Size: 2349 256 1390
Fragments arranged by size:
2349 1390 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3995
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3995
AvaI C'yCGr_G
Cuts at: 451 1015 451
Size: 564 3431
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 726 759 726
Size: 33 3962
BamHI G'GATC_C
Cuts at: 1020 1020
Size: 3995
BanI G'GyrC_C
Cuts at: 131 217 1011 1425 1673 1708 131
Size: 86 794 414 248 35 2418
Fragments arranged by size:
2418 794 414 248 86 35
BanII G_rGCy'C
Cuts at: 328 383 632 1009 2039 328
Size: 55 249 377 1030 2284
Fragments arranged by size:
2284 1030 377 249 55
BbeI G_GCGC'C
Cuts at: 221 1429 1677 221
Size: 1208 248 2539
Fragments arranged by size:
2539 1208 248
BbsI GAAGACnn'nnnn_
Cuts at: 776 776
Size: 3995
BbvCI CC'TCA_GC
Cuts at: 833 833
Size: 3995
BciVI GTATCCnnnnn_n'
Cuts at: 754 1893 2654 3995 754
Size: 1139 761 1341 754
Fragments arranged by size:
1341 1139 761 754
BclI T'GATC_A
Cuts at: 1515 1515
Size: 3995
BglII A'GATC_T
Cuts at: 1510 3547 1510
Size: 2037 1958
BlpI GC'TnA_GC
Cuts at: 486 1107 486
Size: 621 3374
Bme1580I G_kGCm'C
Cuts at: 579 1620 1713 2769 579
Size: 1041 93 1056 1805
Fragments arranged by size:
1805 1056 1041 93
BmrI ACTGGGnnnn_n'
Cuts at: 331 858 1207 1288 1623 3767 331
Size: 527 349 81 335 2144 559
Fragments arranged by size:
2144 559 527 349 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2362 2362
Size: 3995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 685 139
Size: 546 3449
Bpu10I CC'TnA_GC
Cuts at: 280 833 280
Size: 553 3442
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 2050 2542 2840 1171
Size: 879 492 298 2326
Fragments arranged by size:
2326 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 643 3699 643
Size: 3056 939
BsaAI yAC'GTr
Cuts at: 409 1348 409
Size: 939 3056
BsaBI GATnn'nnATC
Cuts at: 6 1509 3546 6
Size: 1503 2037 455
Fragments arranged by size:
2037 1503 455
BsaHI Gr'CG_yC
Cuts at: 218 421 1426 1674 2376 3872 218
Size: 203 1005 248 702 1496 341
Fragments arranged by size:
1496 1005 702 341 248 203
BsaWI w'CCGG_w
Cuts at: 686 1184 1705 2383 2657 2804 3215 686
Size: 498 521 678 274 147 411 1466
Fragments arranged by size:
1466 678 521 498 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 409 439 409
Size: 30 3965
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 286 430 286
Size: 144 3851
BseYI C'CCAG_C
Cuts at: 213 659 1420 2178 2755 3251 213
Size: 446 761 758 577 496 957
Fragments arranged by size:
957 761 758 577 496 446
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 661 449
Size: 212 3783
BsiEI CG_ry'CG
Cuts at: 104 1537 1583 2791 104
Size: 1433 46 1208 1308
Fragments arranged by size:
1433 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 383 632 1009 1787 1977 2769 383
Size: 249 377 778 190 792 1609
Fragments arranged by size:
1609 792 778 377 249 190
BsmBI CGTCTCn'nnnn_
Cuts at: 412 412
Size: 3995
Bsp1286I G_dGCh'C
Cuts at: 328 383 579 632 1009 1620 1713 1787 1977
Size: 55 196 53 377 611 93 74 190
Cuts at: 1977 2039 2769 328
Size: 62 730 1554
Fragments arranged by size:
1554 730 611 377 196 190 93 74
62 55 53
BspEI T'CCGG_A
Cuts at: 3215 3215
Size: 3995
BspHI T'CATG_A
Cuts at: 3171 3171
Size: 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1045 1259 1561 1942 1045
Size: 214 302 381 3098
Fragments arranged by size:
3098 381 302 214
BsrBI CCG'CTC
Cuts at: 50 123 692 2287 2341 3825 3990 50
Size: 73 569 1595 54 1484 165 55
Fragments arranged by size:
1595 1484 569 165 73 55 54
BsrDI GCAATG_nn'
Cuts at: 1907 1907
Size: 3995
BsrFI r'CCGG_y
Cuts at: 1993 2174 2383 1993
Size: 181 209 3605
Fragments arranged by size:
3605 209 181
BssSI C'ACGA_G
Cuts at: 2266 2624 2266
Size: 358 3637
BstAPI GCAn_nnn'nTGC
Cuts at: 363 443 702 1372 363
Size: 80 259 670 2986
Fragments arranged by size:
2986 670 259 80
BstBI TT'CG_AA
Cuts at: 2356 2356
Size: 3995
BstEII G'GTnAC_C
Cuts at: 882 882
Size: 3995
BstYI r'GATC_y
Cuts at: 1 1020 1487 1510 1845 2091 3103 3189 3201
Size: 1019 467 23 335 246 1012 86 12
Cuts at: 3201 3547 1
Size: 346 449
Fragments arranged by size:
1019 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 713 798 107
Size: 606 85 3304
Fragments arranged by size:
3304 606 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1945 2126 2289 1945
Size: 181 163 3651
Fragments arranged by size:
3651 181 163
BtsI GCAGTG_nn'
Cuts at: 586 1238 586
Size: 652 3343
ClaI AT'CG_AT
Cuts at: 264 264
Size: 3995
DraI TTT'AAA
Cuts at: 994 994
Size: 3995
DrdI GACnn_nn'nnGTC
Cuts at: 610 1701 2559 610
Size: 1091 858 2046
Fragments arranged by size:
2046 1091 858
EaeI y'GGCC_r
Cuts at: 461 784 1580 1754 2145 2172 3517 461
Size: 323 796 174 391 27 1345 939
Fragments arranged by size:
1345 939 796 391 323 174 27
EagI C'GGCC_G
Cuts at: 1580 1580
Size: 3995
EarI CTCTTCn'nnn_
Cuts at: 2018 2228 2018
Size: 210 3785
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 337 2203 2513 2659 3805 337
Size: 1866 310 146 1146 527
Fragments arranged by size:
1866 1146 527 310 146
EcoICRI GAG'CTC
Cuts at: 381 630 1007 381
Size: 249 377 3369
Fragments arranged by size:
3369 377 249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 685 1820 2252 2999 3267 139
Size: 546 1135 432 747 268 867
Fragments arranged by size:
1135 867 747 546 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 832 832
Size: 3995
EcoO109I rG'GnC_Cy
Cuts at: 1134 1134
Size: 3995
EcoRI G'AATT_C
Cuts at: 999 999
Size: 3995
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2014 2014
Size: 3995
FspI TGC'GCA
Cuts at: 1776 1776
Size: 3995
HaeII r_GCGC'y
Cuts at: 221 1429 1677 2699 3678 221
Size: 1208 248 1022 979 538
Fragments arranged by size:
1208 1022 979 538 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 257 289 1837 1867 1869 1899 257
Size: 32 1548 30 2 30 2353
Fragments arranged by size:
2353 1548 32 30 30 2
HincII GTy'rAC
Cuts at: 615 1034 615
Size: 419 3576
KasI G'GCGC_C
Cuts at: 217 1425 1673 217
Size: 1208 248 2539
Fragments arranged by size:
2539 1208 248
KpnI G_GTAC'C
Cuts at: 1015 1015
Size: 3995
MluI A'CGCG_T
Cuts at: 3428 3428
Size: 3995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2666 2850 2666
Size: 184 3811
MscI TGG'CCA
Cuts at: 786 1756 786
Size: 970 3025
MslI CAynn'nnrTG
Cuts at: 973 1984 2111 973
Size: 1011 127 2857
Fragments arranged by size:
2857 1011 127
MspA1I CmG'CkG
Cuts at: 240 663 779 915 1106 1420 1780 2793 3038
Size: 423 116 136 191 314 360 1013 245
Cuts at: 3038 240
Size: 1197
Fragments arranged by size:
1197 1013 423 360 314 245 191 136
116
NaeI GCC'GGC
Cuts at: 2176 2176
Size: 3995
NarI GG'CG_CC
Cuts at: 218 1426 1674 218
Size: 1208 248 2539
Fragments arranged by size:
2539 1208 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3995
NdeI CA'TA_TG
Cuts at: 972 972
Size: 3995
NgoMIV G'CCGG_C
Cuts at: 2174 2174
Size: 3995
NspI r_CATG'y
Cuts at: 1048 1548 2455 1048
Size: 500 907 2588
Fragments arranged by size:
2588 907 500
PasI CC'CwG_GG
Cuts at: 492 492
Size: 3995
PciI A'CATG_T
Cuts at: 2451 2451
Size: 3995
PflMI CCAn_nnn'nTGG
Cuts at: 306 306
Size: 3995
PmeI GTTT'AAAC
Cuts at: 994 994
Size: 3995
PmlI CAC'GTG
Cuts at: 409 409
Size: 3995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3134 3166 3134
Size: 32 3963
PspXI vC'TCGA_Gb
Cuts at: 451 451
Size: 3995
PstI C_TGCA'G
Cuts at: 149 707 1042 1727 149
Size: 558 335 685 2417
Fragments arranged by size:
2417 685 558 335
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3995
PvuII CAG'CTG
Cuts at: 240 663 915 1420 1780 240
Size: 423 252 505 360 2455
Fragments arranged by size:
2455 505 423 360 252
RsrII CG'GwC_CG
Cuts at: 2190 2190
Size: 3995
SacI G_AGCT'C
Cuts at: 383 632 1009 383
Size: 249 377 3369
Fragments arranged by size:
3369 377 249
SalI G'TCGA_C
Cuts at: 1032 1032
Size: 3995
SapI GCTCTTCn'nnn_
Cuts at: 2018 2228 2018
Size: 210 3785
SbfI CC_TGCA'GG
Cuts at: 1042 1042
Size: 3995
ScaI AGT'ACT
Cuts at: 3512 3512
Size: 3995
SfcI C'TryA_G
Cuts at: 33 145 224 540 703 1038 1723 2716 2907
Size: 112 79 316 163 335 685 993 191
Cuts at: 2907 33
Size: 1121
Fragments arranged by size:
1121 993 685 335 316 191 163 112
79
SfoI GGC'GCC
Cuts at: 219 1427 1675 219
Size: 1208 248 2539
Fragments arranged by size:
2539 1208 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3995
SmaI CCC'GGG
Cuts at: 1017 1017
Size: 3995
SmlI C'TyrA_G
Cuts at: 451 1150 2065 2557 2819 451
Size: 699 915 492 262 1627
Fragments arranged by size:
1627 915 699 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3995
SphI G_CATG'C
Cuts at: 1048 1548 1048
Size: 500 3495
StuI AGG'CCT
Cuts at: 982 982
Size: 3995
StyI C'CwwG_G
Cuts at: 107 1129 1483 107
Size: 1022 354 2619
Fragments arranged by size:
2619 1022 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1578 1711 2170 2379 1578
Size: 133 459 209 3194
Fragments arranged by size:
3194 459 209 133
TatI w'GTAC_w
Cuts at: 3510 3510
Size: 3995
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 409 441 3512 3544 409
Size: 32 3071 32 860
Fragments arranged by size:
3071 860 32 32
Tth111I GACn'n_nGTC
Cuts at: 1792 1792
Size: 3995
XbaI T'CTAG_A
Cuts at: 1026 1026
Size: 3995
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 210 1490 114
Size: 96 1280 2619
Fragments arranged by size:
2619 1280 96
XhoI C'TCGA_G
Cuts at: 451 451
Size: 3995
XmaI C'CCGG_G
Cuts at: 1015 1015
Size: 3995
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PmeI PmlI PpiI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmI BsrGI BssHII BstXI BstZ17I Bsu36I CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PfoI PpuMI PshAI PsiI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI