Restriction map for
pF1KE0941
(Circular) (Six-base) MAPSORT of: pF1KE0941.seq Check: 5741 from: 1 to: 3917
pF1KE0941 3917 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 955 955
Size: 3917
Acc65I G'GTAC_C
Cuts at: 134 933 134
Size: 799 3118
AclI AA'CG_TT
Cuts at: 3757 3757
Size: 3917
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1742 2174 2921 3189 568
Size: 1174 432 747 268 1296
Fragments arranged by size:
1296 1174 747 432 268
AflIII A'CryG_T
Cuts at: 1269 2373 3350 1269
Size: 1104 977 1836
Fragments arranged by size:
1836 1104 977
AgeI A'CCGG_T
Cuts at: 2305 2305
Size: 3917
AlwNI CAG_nnn'CTG
Cuts at: 828 1222 2789 828
Size: 394 1567 1956
Fragments arranged by size:
1956 1567 394
ApaLI G'TGCA_C
Cuts at: 2687 2687
Size: 3917
ApoI r'AATT_y
Cuts at: 921 3270 3526 921
Size: 2349 256 1312
Fragments arranged by size:
2349 1312 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3917
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3917
AvaI C'yCGr_G
Cuts at: 275 640 937 275
Size: 365 297 3255
Fragments arranged by size:
3255 365 297
BamHI G'GATC_C
Cuts at: 942 942
Size: 3917
BanI G'GyrC_C
Cuts at: 134 933 1347 1595 1630 134
Size: 799 414 248 35 2421
Fragments arranged by size:
2421 799 414 248 35
BanII G_rGCy'C
Cuts at: 284 377 931 1961 284
Size: 93 554 1030 2240
Fragments arranged by size:
2240 1030 554 93
BbeI G_GCGC'C
Cuts at: 1351 1599 1351
Size: 248 3669
BbvCI CC'TCA_GC
Cuts at: 380 380
Size: 3917
BciVI GTATCCnnnnn_n'
Cuts at: 1815 2576 3917 1815
Size: 761 1341 1815
Fragments arranged by size:
1815 1341 761
BclI T'GATC_A
Cuts at: 1437 1437
Size: 3917
BglII A'GATC_T
Cuts at: 1432 3469 1432
Size: 2037 1880
BlpI GC'TnA_GC
Cuts at: 601 1029 601
Size: 428 3489
Bme1580I G_kGCm'C
Cuts at: 1542 1635 2691 1542
Size: 93 1056 2768
Fragments arranged by size:
2768 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1129 1210 1545 3689 1129
Size: 81 335 2144 1357
Fragments arranged by size:
2144 1357 335 81
BmtI G_CTAG'C
Cuts at: 904 904
Size: 3917
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2284 2284
Size: 3917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 708 358
Size: 350 3567
Bpu10I CC'TnA_GC
Cuts at: 380 380
Size: 3917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1093 1972 2464 2762 817
Size: 276 879 492 298 1972
Fragments arranged by size:
1972 879 492 298 276
BsaI GGTCTCn'nnnn_
Cuts at: 3621 3621
Size: 3917
BsaAI yAC'GTr
Cuts at: 1270 1270
Size: 3917
BsaBI GATnn'nnATC
Cuts at: 6 1431 3468 6
Size: 1425 2037 455
Fragments arranged by size:
2037 1425 455
BsaHI Gr'CG_yC
Cuts at: 1348 1596 2298 3794 1348
Size: 248 702 1496 1471
Fragments arranged by size:
1496 1471 702 248
BsaWI w'CCGG_w
Cuts at: 1106 1627 2305 2579 2726 3137 1106
Size: 521 678 274 147 411 1886
Fragments arranged by size:
1886 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 377 407 681 711 377
Size: 30 274 30 3583
Fragments arranged by size:
3583 274 30 30
BseYI C'CCAG_C
Cuts at: 141 1342 2100 2677 3173 141
Size: 1201 758 577 496 885
Fragments arranged by size:
1201 885 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 815 1459 1505 2713 104
Size: 711 644 46 1208 1308
Fragments arranged by size:
1308 1208 711 644 46
BsiHKAI G_wGCw'C
Cuts at: 284 377 931 1709 1899 2691 284
Size: 93 554 778 190 792 1510
Fragments arranged by size:
1510 792 778 554 190 93
BsmI GAATG_Cn'
Cuts at: 656 656
Size: 3917
Bsp1286I G_dGCh'C
Cuts at: 284 377 931 1542 1635 1709 1899 1961 2691
Size: 93 554 611 93 74 190 62 730
Cuts at: 2691 284
Size: 1510
Fragments arranged by size:
1510 730 611 554 190 93 93 74
62
BspEI T'CCGG_A
Cuts at: 3137 3137
Size: 3917
BspHI T'CATG_A
Cuts at: 3093 3093
Size: 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 836 967 1181 1483 1864 836
Size: 131 214 302 381 2889
Fragments arranged by size:
2889 381 302 214 131
BsrBI CCG'CTC
Cuts at: 50 2209 2263 3747 3912 50
Size: 2159 54 1484 165 55
Fragments arranged by size:
2159 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1829 1829
Size: 3917
BsrFI r'CCGG_y
Cuts at: 1915 2096 2305 1915
Size: 181 209 3527
Fragments arranged by size:
3527 209 181
BssSI C'ACGA_G
Cuts at: 2188 2546 2188
Size: 358 3559
BstAPI GCAn_nnn'nTGC
Cuts at: 1294 1294
Size: 3917
BstBI TT'CG_AA
Cuts at: 2278 2278
Size: 3917
BstXI CCAn_nnnn'nTGG
Cuts at: 490 490
Size: 3917
BstYI r'GATC_y
Cuts at: 1 942 1409 1432 1767 2013 3025 3111 3123
Size: 941 467 23 335 246 1012 86 12
Cuts at: 3123 3469 1
Size: 346 449
Fragments arranged by size:
1012 941 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 483 895 107
Size: 376 412 3129
Fragments arranged by size:
3129 412 376
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1867 2048 2211 1867
Size: 181 163 3573
Fragments arranged by size:
3573 181 163
BtsI GCAGTG_nn'
Cuts at: 421 1160 421
Size: 739 3178
DraI TTT'AAA
Cuts at: 916 916
Size: 3917
DrdI GACnn_nn'nnGTC
Cuts at: 1623 2481 1623
Size: 858 3059
EaeI y'GGCC_r
Cuts at: 812 1502 1676 2067 2094 3439 812
Size: 690 174 391 27 1345 1290
Fragments arranged by size:
1345 1290 690 391 174 27
EagI C'GGCC_G
Cuts at: 812 1502 812
Size: 690 3227
EarI CTCTTCn'nnn_
Cuts at: 245 454 1940 2150 245
Size: 209 1486 210 2012
Fragments arranged by size:
2012 1486 210 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2125 2435 2581 3727 2125
Size: 310 146 1146 2315
Fragments arranged by size:
2315 1146 310 146
EcoICRI GAG'CTC
Cuts at: 282 375 929 282
Size: 93 554 3270
Fragments arranged by size:
3270 554 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 568 708 1742 2174 2921 3189 358
Size: 210 140 1034 432 747 268 1086
Fragments arranged by size:
1086 1034 747 432 268 210 140
EcoO109I rG'GnC_Cy
Cuts at: 164 444 507 825 1056 164
Size: 280 63 318 231 3025
Fragments arranged by size:
3025 318 280 231 63
EcoRI G'AATT_C
Cuts at: 921 921
Size: 3917
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 465 1936 465
Size: 1471 2446
FspI TGC'GCA
Cuts at: 1698 1698
Size: 3917
HaeII r_GCGC'y
Cuts at: 1351 1599 2621 3600 1351
Size: 248 1022 979 1668
Fragments arranged by size:
1668 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 377 409 1759 1789 1791 1821 377
Size: 32 1350 30 2 30 2473
Fragments arranged by size:
2473 1350 32 30 30 2
HincII GTy'rAC
Cuts at: 956 956
Size: 3917
KasI G'GCGC_C
Cuts at: 1347 1595 1347
Size: 248 3669
KpnI G_GTAC'C
Cuts at: 138 937 138
Size: 799 3118
MfeI C'AATT_G
Cuts at: 872 872
Size: 3917
MluI A'CGCG_T
Cuts at: 3350 3350
Size: 3917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2588 2772 2588
Size: 184 3733
MscI TGG'CCA
Cuts at: 1678 1678
Size: 3917
MslI CAynn'nnrTG
Cuts at: 322 488 697 1906 2033 322
Size: 166 209 1209 127 2206
Fragments arranged by size:
2206 1209 209 166 127
MspA1I CmG'CkG
Cuts at: 141 1028 1342 1702 2715 2960 141
Size: 887 314 360 1013 245 1098
Fragments arranged by size:
1098 1013 887 360 314 245
NaeI GCC'GGC
Cuts at: 2098 2098
Size: 3917
NarI GG'CG_CC
Cuts at: 1348 1596 1348
Size: 248 3669
NcoI C'CATG_G
Cuts at: 107 483 895 107
Size: 376 412 3129
Fragments arranged by size:
3129 412 376
NgoMIV G'CCGG_C
Cuts at: 2096 2096
Size: 3917
NheI G'CTAG_C
Cuts at: 900 900
Size: 3917
NspI r_CATG'y
Cuts at: 970 1470 2377 970
Size: 500 907 2510
Fragments arranged by size:
2510 907 500
PciI A'CATG_T
Cuts at: 2373 2373
Size: 3917
PfoI T'CCnGG_A
Cuts at: 842 842
Size: 3917
PmeI GTTT'AAAC
Cuts at: 916 916
Size: 3917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3056 3088 3056
Size: 32 3885
PpuMI rG'GwC_Cy
Cuts at: 164 507 825 164
Size: 343 318 3256
Fragments arranged by size:
3256 343 318
PstI C_TGCA'G
Cuts at: 653 964 1649 653
Size: 311 685 2921
Fragments arranged by size:
2921 685 311
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3917
PvuII CAG'CTG
Cuts at: 141 1342 1702 141
Size: 1201 360 2356
Fragments arranged by size:
2356 1201 360
RsrII CG'GwC_CG
Cuts at: 2112 2112
Size: 3917
SacI G_AGCT'C
Cuts at: 284 377 931 284
Size: 93 554 3270
Fragments arranged by size:
3270 554 93
SalI G'TCGA_C
Cuts at: 954 954
Size: 3917
SapI GCTCTTCn'nnn_
Cuts at: 1940 2150 1940
Size: 210 3707
SbfI CC_TGCA'GG
Cuts at: 964 964
Size: 3917
ScaI AGT'ACT
Cuts at: 3434 3434
Size: 3917
SfcI C'TryA_G
Cuts at: 33 649 960 1645 2638 2829 33
Size: 616 311 685 993 191 1121
Fragments arranged by size:
1121 993 685 616 311 191
SfoI GGC'GCC
Cuts at: 1349 1597 1349
Size: 248 3669
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3917
SmaI CCC'GGG
Cuts at: 939 939
Size: 3917
SmlI C'TyrA_G
Cuts at: 832 1072 1987 2479 2741 832
Size: 240 915 492 262 2008
Fragments arranged by size:
2008 915 492 262 240
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3917
SphI G_CATG'C
Cuts at: 970 1470 970
Size: 500 3417
StyI C'CwwG_G
Cuts at: 107 440 483 572 895 1051 1405 107
Size: 333 43 89 323 156 354 2619
Fragments arranged by size:
2619 354 333 323 156 89 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 685 1500 1633 2092 2301 685
Size: 815 133 459 209 2301
Fragments arranged by size:
2301 815 459 209 133
TatI w'GTAC_w
Cuts at: 622 3432 622
Size: 2810 1107
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 681 713 3434 3466 681
Size: 32 2721 32 1132
Fragments arranged by size:
2721 1132 32 32
Tth111I GACn'n_nGTC
Cuts at: 1714 1714
Size: 3917
XbaI T'CTAG_A
Cuts at: 948 948
Size: 3917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1412 1412
Size: 3917
XmaI C'CCGG_G
Cuts at: 937 937
Size: 3917
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NdeI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI