Restriction map for
pF1KE0936
(Circular) (Six-base) MAPSORT of: pF1KE0936.seq Check: 2599 from: 1 to: 4367
pF1KE0936 4367 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 158 374 1405 158
Size: 216 1031 3120
Fragments arranged by size:
3120 1031 216
Acc65I G'GTAC_C
Cuts at: 183 1383 183
Size: 1200 3167
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 340 1165 2192 2624 3371 3639 237
Size: 103 825 1027 432 747 268 965
Fragments arranged by size:
1027 965 825 747 432 268 103
AflIII A'CryG_T
Cuts at: 1719 2823 3800 1719
Size: 1104 977 2286
Fragments arranged by size:
2286 1104 977
AgeI A'CCGG_T
Cuts at: 2755 2755
Size: 4367
AlwNI CAG_nnn'CTG
Cuts at: 1075 1341 1672 3239 1075
Size: 266 331 1567 2203
Fragments arranged by size:
2203 1567 331 266
ApaI G_GGCC'C
Cuts at: 599 599
Size: 4367
ApaLI G'TGCA_C
Cuts at: 3137 3137
Size: 4367
ApoI r'AATT_y
Cuts at: 450 1371 3720 3976 450
Size: 921 2349 256 841
Fragments arranged by size:
2349 921 841 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 140 1040 1387 140
Size: 900 347 3120
Fragments arranged by size:
3120 900 347
BamHI G'GATC_C
Cuts at: 1392 1392
Size: 4367
BanI G'GyrC_C
Cuts at: 183 1383 1797 2045 2080 183
Size: 1200 414 248 35 2470
Fragments arranged by size:
2470 1200 414 248 35
BanII G_rGCy'C
Cuts at: 599 744 769 1162 1177 1298 1381 2411 599
Size: 145 25 393 15 121 83 1030 2555
Fragments arranged by size:
2555 1030 393 145 121 83 25 15
BbeI G_GCGC'C
Cuts at: 1801 2049 1801
Size: 248 4119
BbsI GAAGACnn'nnnn_
Cuts at: 534 836 534
Size: 302 4065
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3026 4367 2265
Size: 761 1341 2265
Fragments arranged by size:
2265 1341 761
BclI T'GATC_A
Cuts at: 293 1887 293
Size: 1594 2773
BglI GCCn_nnn'nGGC
Cuts at: 883 883
Size: 4367
BglII A'GATC_T
Cuts at: 1882 3919 1882
Size: 2037 2330
BlpI GC'TnA_GC
Cuts at: 1479 1479
Size: 4367
Bme1580I G_kGCm'C
Cuts at: 599 1992 2085 3141 599
Size: 1393 93 1056 1825
Fragments arranged by size:
1825 1393 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 673 707 848 957 1579 1660 1995 4139 673
Size: 34 141 109 622 81 335 2144 901
Fragments arranged by size:
2144 901 622 335 141 109 81 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 928 2734 928
Size: 1806 2561
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 456 831 860 456
Size: 375 29 3963
Fragments arranged by size:
3963 375 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1543 2422 2914 3212 1543
Size: 879 492 298 2698
Fragments arranged by size:
2698 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4071 4071
Size: 4367
BsaAI yAC'GTr
Cuts at: 1720 1720
Size: 4367
BsaBI GATnn'nnATC
Cuts at: 6 223 517 1881 3918 6
Size: 217 294 1364 2037 455
Fragments arranged by size:
2037 1364 455 294 217
BsaHI Gr'CG_yC
Cuts at: 1325 1798 2046 2748 4244 1325
Size: 473 248 702 1496 1448
Fragments arranged by size:
1496 1448 702 473 248
BsaWI w'CCGG_w
Cuts at: 1256 1556 2077 2755 3029 3176 3587 1256
Size: 300 521 678 274 147 411 2036
Fragments arranged by size:
2036 678 521 411 300 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 696 726 751 781 696
Size: 30 25 30 4282
Fragments arranged by size:
4282 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 752 920 960 1056 1294 1327 752
Size: 168 40 96 238 33 3792
Fragments arranged by size:
3792 238 168 96 40 33
BseYI C'CCAG_C
Cuts at: 796 1213 1792 2550 3127 3623 796
Size: 417 579 758 577 496 1540
Fragments arranged by size:
1540 758 579 577 496 417
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 508
Size: 4367
BsiEI CG_ry'CG
Cuts at: 104 1909 1955 3163 104
Size: 1805 46 1208 1308
Fragments arranged by size:
1805 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 769 1298 1381 2159 2349 3141 769
Size: 529 83 778 190 792 1995
Fragments arranged by size:
1995 792 778 529 190 83
BsmBI CGTCTCn'nnnn_
Cuts at: 648 648
Size: 4367
Bsp1286I G_dGCh'C
Cuts at: 599 744 769 1162 1177 1298 1381 1992 2085
Size: 145 25 393 15 121 83 611 93
Cuts at: 2085 2159 2349 2411 3141 599
Size: 74 190 62 730 1825
Fragments arranged by size:
1825 730 611 393 190 145 121 93
83 74 62 25 15
BspEI T'CCGG_A
Cuts at: 3587 3587
Size: 4367
BspHI T'CATG_A
Cuts at: 446 3543 446
Size: 3097 1270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 276 1417 1631 1933 2314 276
Size: 1141 214 302 381 2329
Fragments arranged by size:
2329 1141 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1095 2659 2713 4197 4362 50
Size: 1045 1564 54 1484 165 55
Fragments arranged by size:
1564 1484 1045 165 55 54
BsrDI GCAATG_nn'
Cuts at: 431 2279 431
Size: 1848 2519
BsrFI r'CCGG_y
Cuts at: 2365 2546 2755 2365
Size: 181 209 3977
Fragments arranged by size:
3977 209 181
BssSI C'ACGA_G
Cuts at: 2638 2996 2638
Size: 358 4009
BstAPI GCAn_nnn'nTGC
Cuts at: 1744 1744
Size: 4367
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstYI r'GATC_y
Cuts at: 1 1011 1271 1392 1859 1882 2217 2463 3475
Size: 1010 260 121 467 23 335 246 1012
Cuts at: 3475 3561 3573 3919 1
Size: 86 12 346 449
Fragments arranged by size:
1012 1010 467 449 346 335 260 246
121 86 23 12
BtgI C'CryG_G
Cuts at: 107 1358 107
Size: 1251 3116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2317 2498 2661 2317
Size: 181 163 4023
Fragments arranged by size:
4023 181 163
BtsI GCAGTG_nn'
Cuts at: 236 724 1610 236
Size: 488 886 2993
Fragments arranged by size:
2993 886 488
DraI TTT'AAA
Cuts at: 1366 1366
Size: 4367
DraIII CAC_nnn'GTG
Cuts at: 1305 1305
Size: 4367
DrdI GACnn_nn'nnGTC
Cuts at: 867 2073 2931 867
Size: 1206 858 2303
Fragments arranged by size:
2303 1206 858
EaeI y'GGCC_r
Cuts at: 110 198 587 782 823 938 1077 1952 2126
Size: 88 389 195 41 115 139 875 174
Cuts at: 2126 2517 2544 3889 110
Size: 391 27 1345 588
Fragments arranged by size:
1345 875 588 391 389 195 174 139
115 88 41 27
EagI C'GGCC_G
Cuts at: 1952 1952
Size: 4367
EarI CTCTTCn'nnn_
Cuts at: 395 807 2390 2600 395
Size: 412 1583 210 2162
Fragments arranged by size:
2162 1583 412 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 917 2575 2885 3031 4177 917
Size: 1658 310 146 1146 1107
Fragments arranged by size:
1658 1146 1107 310 146
EcoICRI GAG'CTC
Cuts at: 767 1296 1379 767
Size: 529 83 3755
Fragments arranged by size:
3755 529 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 340 456 831 860 1165 2192 2624 3371
Size: 103 116 375 29 305 1027 432 747
Cuts at: 3371 3639 237
Size: 268 965
Fragments arranged by size:
1027 965 747 432 375 305 268 116
103 29
EcoNI CCTnn'n_nnAGG
Cuts at: 916 1279 916
Size: 363 4004
EcoO109I rG'GnC_Cy
Cuts at: 466 595 860 1037 1506 466
Size: 129 265 177 469 3327
Fragments arranged by size:
3327 469 265 177 129
EcoRI G'AATT_C
Cuts at: 450 1371 450
Size: 921 3446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4367
FspI TGC'GCA
Cuts at: 2148 2148
Size: 4367
HaeII r_GCGC'y
Cuts at: 638 1801 2049 3071 4050 638
Size: 1163 248 1022 979 955
Fragments arranged by size:
1163 1022 979 955 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 751 783 2209 2239 2241 2271 751
Size: 32 1426 30 2 30 2847
Fragments arranged by size:
2847 1426 32 30 30 2
HincII GTy'rAC
Cuts at: 1406 1406
Size: 4367
KasI G'GCGC_C
Cuts at: 1797 2045 1797
Size: 248 4119
KpnI G_GTAC'C
Cuts at: 187 1387 187
Size: 1200 3167
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 243 843 3038 3222 243
Size: 600 2195 184 1388
Fragments arranged by size:
2195 1388 600 184
MscI TGG'CCA
Cuts at: 112 200 940 2128 112
Size: 88 740 1188 2351
Fragments arranged by size:
2351 1188 740 88
MslI CAynn'nnrTG
Cuts at: 2356 2483 2356
Size: 127 4240
MspA1I CmG'CkG
Cuts at: 787 893 1478 1792 2152 3165 3410 787
Size: 106 585 314 360 1013 245 1744
Fragments arranged by size:
1744 1013 585 360 314 245 106
NaeI GCC'GGC
Cuts at: 2548 2548
Size: 4367
NarI GG'CG_CC
Cuts at: 1798 2046 1798
Size: 248 4119
NcoI C'CATG_G
Cuts at: 107 107
Size: 4367
NgoMIV G'CCGG_C
Cuts at: 2546 2546
Size: 4367
NspI r_CATG'y
Cuts at: 1420 1920 2827 1420
Size: 500 907 2960
Fragments arranged by size:
2960 907 500
PasI CC'CwG_GG
Cuts at: 744 1088 744
Size: 344 4023
PciI A'CATG_T
Cuts at: 2823 2823
Size: 4367
PflMI CCAn_nnn'nTGG
Cuts at: 697 697
Size: 4367
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 4335
PpuMI rG'GwC_Cy
Cuts at: 860 1037 860
Size: 177 4190
PspOMI G'GGCC_C
Cuts at: 595 595
Size: 4367
PstI C_TGCA'G
Cuts at: 630 1414 2099 630
Size: 784 685 2898
Fragments arranged by size:
2898 784 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 1792 2152 1792
Size: 360 4007
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 769 1298 1381 769
Size: 529 83 3755
Fragments arranged by size:
3755 529 83
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SanDI GG'GwC_CC
Cuts at: 1037 1037
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 2390 2600 2390
Size: 210 4157
SbfI CC_TGCA'GG
Cuts at: 1414 1414
Size: 4367
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SfcI C'TryA_G
Cuts at: 33 626 1410 2095 3088 3279 33
Size: 593 784 685 993 191 1121
Fragments arranged by size:
1121 993 784 685 593 191
SfoI GGC'GCC
Cuts at: 1799 2047 1799
Size: 248 4119
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 142 1389 142
Size: 1247 3120
SmlI C'TyrA_G
Cuts at: 1522 2437 2929 3191 1522
Size: 915 492 262 2698
Fragments arranged by size:
2698 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 1420 1920 1420
Size: 500 3867
SrfI GCCC'GGGC
Cuts at: 142 142
Size: 4367
StuI AGG'CCT
Cuts at: 911 911
Size: 4367
StyI C'CwwG_G
Cuts at: 107 1501 1855 107
Size: 1394 354 2619
Fragments arranged by size:
2619 1394 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 708 1950 2083 2542 2751 708
Size: 1242 133 459 209 2324
Fragments arranged by size:
2324 1242 459 209 133
TatI w'GTAC_w
Cuts at: 323 3882 323
Size: 3559 808
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 665 697 3884 3916 665
Size: 32 3187 32 1116
Fragments arranged by size:
3187 1116 32 32
Tth111I GACn'n_nGTC
Cuts at: 2164 2164
Size: 4367
XbaI T'CTAG_A
Cuts at: 570 1398 570
Size: 828 3539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 777 1862 777
Size: 1085 3282
XmaI C'CCGG_G
Cuts at: 140 1387 140
Size: 1247 3120
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SspI SwaI XhoI XmnI ZraI