Restriction map for
pF1KE0935
(Circular) (Six-base) MAPSORT of: pF1KE0935.seq Check: 1148 from: 1 to: 4367
pF1KE0935 4367 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AccI GT'mk_AC
Cuts at: 146 1405 146
Size: 1259 3108
Acc65I G'GTAC_C
Cuts at: 1383 1383
Size: 4367
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 2192 2624 3371 3639 397
Size: 1795 432 747 268 1125
Fragments arranged by size:
1795 1125 747 432 268
AflIII A'CryG_T
Cuts at: 1719 2823 3800 1719
Size: 1104 977 2286
Fragments arranged by size:
2286 1104 977
AgeI A'CCGG_T
Cuts at: 2755 2755
Size: 4367
AleI CACnn'nnGTG
Cuts at: 857 941 857
Size: 84 4283
AlwNI CAG_nnn'CTG
Cuts at: 1672 3239 1672
Size: 1567 2800
ApaLI G'TGCA_C
Cuts at: 3137 3137
Size: 4367
ApoI r'AATT_y
Cuts at: 386 613 764 848 932 1016 1184 1268 1350
Size: 227 151 84 84 84 168 84 82
Cuts at: 1350 1371 3720 3976 386
Size: 21 2349 256 777
Fragments arranged by size:
2349 777 256 227 168 151 84 84
84 84 82 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 1387 1387
Size: 4367
BamHI G'GATC_C
Cuts at: 1392 1392
Size: 4367
BanI G'GyrC_C
Cuts at: 1383 1797 2045 2080 1383
Size: 414 248 35 3670
Fragments arranged by size:
3670 414 248 35
BanII G_rGCy'C
Cuts at: 299 1078 1381 2411 299
Size: 779 303 1030 2255
Fragments arranged by size:
2255 1030 779 303
BbeI G_GCGC'C
Cuts at: 1801 2049 1801
Size: 248 4119
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3026 4367 2265
Size: 761 1341 2265
Fragments arranged by size:
2265 1341 761
BclI T'GATC_A
Cuts at: 183 1887 183
Size: 1704 2663
BglII A'GATC_T
Cuts at: 549 1882 3919 549
Size: 1333 2037 997
Fragments arranged by size:
2037 1333 997
BlpI GC'TnA_GC
Cuts at: 1479 1479
Size: 4367
Bme1580I G_kGCm'C
Cuts at: 1992 2085 3141 1992
Size: 93 1056 3218
Fragments arranged by size:
3218 1056 93
BmgBI CAC'GTC
Cuts at: 439 439
Size: 4367
BmrI ACTGGGnnnn_n'
Cuts at: 402 1034 1202 1368 1579 1660 1995 4139 402
Size: 632 168 166 211 81 335 2144 630
Fragments arranged by size:
2144 632 630 335 211 168 166 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 438 438
Size: 4367
Bpu10I CC'TnA_GC
Cuts at: 113 113
Size: 4367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 726 1543 2422 2914 3212 726
Size: 817 879 492 298 1881
Fragments arranged by size:
1881 879 817 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 352 4071 352
Size: 3719 648
BsaAI yAC'GTr
Cuts at: 1720 1720
Size: 4367
BsaBI GATnn'nnATC
Cuts at: 6 1881 3918 6
Size: 1875 2037 455
Fragments arranged by size:
2037 1875 455
BsaHI Gr'CG_yC
Cuts at: 1798 2046 2748 4244 1798
Size: 248 702 1496 1921
Fragments arranged by size:
1921 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1556 2077 2755 3029 3176 3587 1556
Size: 521 678 274 147 411 2336
Fragments arranged by size:
2336 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 286 316 286
Size: 30 4337
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 175
Size: 4367
BseYI C'CCAG_C
Cuts at: 1792 2550 3127 3623 1792
Size: 758 577 496 2536
Fragments arranged by size:
2536 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1909 1955 3163 104
Size: 1805 46 1208 1308
Fragments arranged by size:
1805 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 454 1078 1381 2159 2349 3141 454
Size: 624 303 778 190 792 1680
Fragments arranged by size:
1680 792 778 624 303 190
BsmI GAATG_Cn'
Cuts at: 175 246 964 973 175
Size: 71 718 9 3569
Fragments arranged by size:
3569 718 71 9
Bsp1286I G_dGCh'C
Cuts at: 299 454 1078 1381 1992 2085 2159 2349 2411
Size: 155 624 303 611 93 74 190 62
Cuts at: 2411 3141 299
Size: 730 1525
Fragments arranged by size:
1525 730 624 611 303 190 155 93
74 62
BspEI T'CCGG_A
Cuts at: 3587 3587
Size: 4367
BspHI T'CATG_A
Cuts at: 543 3543 543
Size: 3000 1367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1417 1631 1933 2314 1417
Size: 214 302 381 3470
Fragments arranged by size:
3470 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2659 2713 4197 4362 50
Size: 2609 54 1484 165 55
Fragments arranged by size:
2609 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1065 2279 1065
Size: 1214 3153
BsrFI r'CCGG_y
Cuts at: 2365 2546 2755 2365
Size: 181 209 3977
Fragments arranged by size:
3977 209 181
BssSI C'ACGA_G
Cuts at: 2638 2996 2638
Size: 358 4009
BstAPI GCAn_nnn'nTGC
Cuts at: 968 1744 968
Size: 776 3591
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstYI r'GATC_y
Cuts at: 1 549 1392 1859 1882 2217 2463 3475 3561
Size: 548 843 467 23 335 246 1012 86
Cuts at: 3561 3573 3919 1
Size: 12 346 449
Fragments arranged by size:
1012 843 548 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4367
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2317 2498 2661 2317
Size: 181 163 4023
Fragments arranged by size:
4023 181 163
BtsI GCAGTG_nn'
Cuts at: 1610 1610
Size: 4367
DraI TTT'AAA
Cuts at: 1366 1366
Size: 4367
DrdI GACnn_nn'nnGTC
Cuts at: 2073 2931 2073
Size: 858 3509
EaeI y'GGCC_r
Cuts at: 334 1952 2126 2517 2544 3889 334
Size: 1618 174 391 27 1345 812
Fragments arranged by size:
1618 1345 812 391 174 27
EagI C'GGCC_G
Cuts at: 1952 1952
Size: 4367
EarI CTCTTCn'nnn_
Cuts at: 2390 2600 2390
Size: 210 4157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2575 2885 3031 4177 2575
Size: 310 146 1146 2765
Fragments arranged by size:
2765 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1076 1379 1076
Size: 303 4064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 438 2192 2624 3371 3639 397
Size: 41 1754 432 747 268 1125
Fragments arranged by size:
1754 1125 747 432 268 41
EcoO109I rG'GnC_Cy
Cuts at: 1506 1506
Size: 4367
EcoRI G'AATT_C
Cuts at: 848 932 1016 1184 1268 1371 848
Size: 84 84 168 84 103 3844
Fragments arranged by size:
3844 168 103 84 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 801 2386 801
Size: 1585 2782
FspI TGC'GCA
Cuts at: 2148 2148
Size: 4367
HaeII r_GCGC'y
Cuts at: 1801 2049 3071 4050 1801
Size: 248 1022 979 2118
Fragments arranged by size:
2118 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 555 587 2209 2239 2241 2271 555
Size: 32 1622 30 2 30 2651
Fragments arranged by size:
2651 1622 32 30 30 2
HincII GTy'rAC
Cuts at: 1406 1406
Size: 4367
KasI G'GCGC_C
Cuts at: 1797 2045 1797
Size: 248 4119
KpnI G_GTAC'C
Cuts at: 1387 1387
Size: 4367
MfeI C'AATT_G
Cuts at: 529 529
Size: 4367
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 3038 3222 194
Size: 2844 184 1339
Fragments arranged by size:
2844 1339 184
MscI TGG'CCA
Cuts at: 336 2128 336
Size: 1792 2575
MslI CAynn'nnrTG
Cuts at: 857 941 1109 1277 2356 2483 857
Size: 84 168 168 1079 127 2741
Fragments arranged by size:
2741 1079 168 168 127 84
MspA1I CmG'CkG
Cuts at: 1478 1792 2152 3165 3410 1478
Size: 314 360 1013 245 2435
Fragments arranged by size:
2435 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2548 2548
Size: 4367
NarI GG'CG_CC
Cuts at: 1798 2046 1798
Size: 248 4119
NcoI C'CATG_G
Cuts at: 107 107
Size: 4367
NgoMIV G'CCGG_C
Cuts at: 2546 2546
Size: 4367
NspI r_CATG'y
Cuts at: 1420 1920 2827 1420
Size: 500 907 2960
Fragments arranged by size:
2960 907 500
PciI A'CATG_T
Cuts at: 2823 2823
Size: 4367
PfoI T'CCnGG_A
Cuts at: 341 341
Size: 4367
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 4335
PstI C_TGCA'G
Cuts at: 1414 2099 1414
Size: 685 3682
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 1792 2152 1792
Size: 360 4007
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 1078 1381 1078
Size: 303 4064
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 2390 2600 2390
Size: 210 4157
SbfI CC_TGCA'GG
Cuts at: 1414 1414
Size: 4367
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SexAI A'CCwGG_T
Cuts at: 230 230
Size: 4367
SfcI C'TryA_G
Cuts at: 33 1410 2095 3088 3279 33
Size: 1377 685 993 191 1121
Fragments arranged by size:
1377 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1799 2047 1799
Size: 248 4119
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 1389 1389
Size: 4367
SmlI C'TyrA_G
Cuts at: 741 1522 2437 2929 3191 741
Size: 781 915 492 262 1917
Fragments arranged by size:
1917 915 781 492 262
SpeI A'CTAG_T
Cuts at: 67 1276 67
Size: 1209 3158
SphI G_CATG'C
Cuts at: 1420 1920 1420
Size: 500 3867
StuI AGG'CCT
Cuts at: 979 979
Size: 4367
StyI C'CwwG_G
Cuts at: 107 1501 1855 107
Size: 1394 354 2619
Fragments arranged by size:
2619 1394 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1950 2083 2542 2751 1950
Size: 133 459 209 3566
Fragments arranged by size:
3566 459 209 133
TatI w'GTAC_w
Cuts at: 1355 3882 1355
Size: 2527 1840
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 559 591 3884 3916 559
Size: 32 3293 32 1010
Fragments arranged by size:
3293 1010 32 32
Tth111I GACn'n_nGTC
Cuts at: 2164 2164
Size: 4367
XbaI T'CTAG_A
Cuts at: 1398 1398
Size: 4367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1862 1862
Size: 4367
XmaI C'CCGG_G
Cuts at: 1387 1387
Size: 4367
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI