Restriction map for
pF1KE0932
(Circular) (Six-base) MAPSORT of: pF1KE0932.seq Check: 72 from: 1 to: 4346
pF1KE0932 4346 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1112 1384 1112
Size: 272 4074
Acc65I G'GTAC_C
Cuts at: 1362 1362
Size: 4346
AclI AA'CG_TT
Cuts at: 4186 4186
Size: 4346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2171 2603 3350 3618 2171
Size: 432 747 268 2899
Fragments arranged by size:
2899 747 432 268
AflIII A'CryG_T
Cuts at: 1698 2802 3779 1698
Size: 1104 977 2265
Fragments arranged by size:
2265 1104 977
AgeI A'CCGG_T
Cuts at: 2734 2734
Size: 4346
AlwNI CAG_nnn'CTG
Cuts at: 673 1651 3218 673
Size: 978 1567 1801
Fragments arranged by size:
1801 1567 978
ApaI G_GGCC'C
Cuts at: 904 904
Size: 4346
ApaLI G'TGCA_C
Cuts at: 781 3116 781
Size: 2335 2011
ApoI r'AATT_y
Cuts at: 721 856 928 1350 3699 3955 721
Size: 135 72 422 2349 256 1112
Fragments arranged by size:
2349 1112 422 256 135 72
AseI AT'TA_AT
Cuts at: 20 20
Size: 4346
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
AvaI C'yCGr_G
Cuts at: 736 1366 736
Size: 630 3716
BamHI G'GATC_C
Cuts at: 562 1371 562
Size: 809 3537
BanI G'GyrC_C
Cuts at: 519 1362 1776 2024 2059 519
Size: 843 414 248 35 2806
Fragments arranged by size:
2806 843 414 248 35
BanII G_rGCy'C
Cuts at: 172 904 1360 2390 172
Size: 732 456 1030 2128
Fragments arranged by size:
2128 1030 732 456
BbeI G_GCGC'C
Cuts at: 523 1780 2028 523
Size: 1257 248 2841
Fragments arranged by size:
2841 1257 248
BbvCI CC'TCA_GC
Cuts at: 441 441
Size: 4346
BciVI GTATCCnnnnn_n'
Cuts at: 1239 2244 3005 4346 1239
Size: 1005 761 1341 1239
Fragments arranged by size:
1341 1239 1005 761
BclI T'GATC_A
Cuts at: 1148 1866 1148
Size: 718 3628
BglII A'GATC_T
Cuts at: 1861 3898 1861
Size: 2037 2309
BlpI GC'TnA_GC
Cuts at: 1458 1458
Size: 4346
Bme1580I G_kGCm'C
Cuts at: 785 904 1971 2064 3120 785
Size: 119 1067 93 1056 2011
Fragments arranged by size:
2011 1067 1056 119 93
BmrI ACTGGGnnnn_n'
Cuts at: 1558 1639 1974 4118 1558
Size: 81 335 2144 1786
Fragments arranged by size:
2144 1786 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 4346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 186
Size: 4346
Bpu10I CC'TnA_GC
Cuts at: 441 441
Size: 4346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1522 2401 2893 3191 1522
Size: 879 492 298 2677
Fragments arranged by size:
2677 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 743 4050 743
Size: 3307 1039
BsaAI yAC'GTr
Cuts at: 1699 1699
Size: 4346
BsaBI GATnn'nnATC
Cuts at: 6 1860 3897 6
Size: 1854 2037 455
Fragments arranged by size:
2037 1854 455
BsaHI Gr'CG_yC
Cuts at: 520 1777 2025 2727 4223 520
Size: 1257 248 702 1496 643
Fragments arranged by size:
1496 1257 702 643 248
BsaWI w'CCGG_w
Cuts at: 1535 2056 2734 3008 3155 3566 1535
Size: 521 678 274 147 411 2315
Fragments arranged by size:
2315 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 240 240
Size: 4346
BseYI C'CCAG_C
Cuts at: 480 657 967 1771 2529 3106 3602 480
Size: 177 310 804 758 577 496 1224
Fragments arranged by size:
1224 804 758 577 496 310 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 610 610
Size: 4346
BsiEI CG_ry'CG
Cuts at: 104 1888 1934 3142 104
Size: 1784 46 1208 1308
Fragments arranged by size:
1784 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 785 1311 1360 2138 2328 3120 785
Size: 526 49 778 190 792 2011
Fragments arranged by size:
2011 792 778 526 190 49
BsmBI CGTCTCn'nnnn_
Cuts at: 459 1069 459
Size: 610 3736
Bsp1286I G_dGCh'C
Cuts at: 172 785 904 1311 1360 1971 2064 2138 2328
Size: 613 119 407 49 611 93 74 190
Cuts at: 2328 2390 3120 172
Size: 62 730 1398
Fragments arranged by size:
1398 730 613 611 407 190 119 93
74 62 49
BspEI T'CCGG_A
Cuts at: 3566 3566
Size: 4346
BspHI T'CATG_A
Cuts at: 3522 3522
Size: 4346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1396 1610 1912 2293 1396
Size: 214 302 381 3449
Fragments arranged by size:
3449 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1127 2638 2692 4176 4341 50
Size: 1077 1511 54 1484 165 55
Fragments arranged by size:
1511 1484 1077 165 55 54
BsrDI GCAATG_nn'
Cuts at: 817 2258 817
Size: 1441 2905
BsrFI r'CCGG_y
Cuts at: 2344 2525 2734 2344
Size: 181 209 3956
Fragments arranged by size:
3956 209 181
BssSI C'ACGA_G
Cuts at: 368 2617 2975 368
Size: 2249 358 1739
Fragments arranged by size:
2249 1739 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1723 1723
Size: 4346
BstBI TT'CG_AA
Cuts at: 2707 2707
Size: 4346
BstYI r'GATC_y
Cuts at: 1 562 1371 1838 1861 2196 2442 3454 3540
Size: 561 809 467 23 335 246 1012 86
Cuts at: 3540 3552 3898 1
Size: 12 346 449
Fragments arranged by size:
1012 809 561 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1113 1113
Size: 4346
BtgI C'CryG_G
Cuts at: 107 107
Size: 4346
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2296 2477 2640 2296
Size: 181 163 4002
Fragments arranged by size:
4002 181 163
BtsI GCAGTG_nn'
Cuts at: 461 1589 461
Size: 1128 3218
DraI TTT'AAA
Cuts at: 944 1345 944
Size: 401 3945
DraIII CAC_nnn'GTG
Cuts at: 220 220
Size: 4346
DrdI GACnn_nn'nnGTC
Cuts at: 2052 2910 2052
Size: 858 3488
EaeI y'GGCC_r
Cuts at: 431 458 532 1101 1931 2105 2496 2523 3868
Size: 27 74 569 830 174 391 27 1345
Cuts at: 3868 431
Size: 909
Fragments arranged by size:
1345 909 830 569 391 174 74 27
27
EagI C'GGCC_G
Cuts at: 1931 1931
Size: 4346
EarI CTCTTCn'nnn_
Cuts at: 781 2369 2579 781
Size: 1588 210 2548
Fragments arranged by size:
2548 1588 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2554 2864 3010 4156 2554
Size: 310 146 1146 2744
Fragments arranged by size:
2744 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1358 1358
Size: 4346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 2171 2603 3350 3618 186
Size: 1985 432 747 268 914
Fragments arranged by size:
1985 914 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 198 414 900 901 1485 198
Size: 216 486 1 584 3059
Fragments arranged by size:
3059 584 486 216 1
EcoRI G'AATT_C
Cuts at: 1350 1350
Size: 4346
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 4346
FspI TGC'GCA
Cuts at: 2127 2127
Size: 4346
HaeII r_GCGC'y
Cuts at: 523 1780 2028 3050 4029 523
Size: 1257 248 1022 979 840
Fragments arranged by size:
1257 1022 979 840 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 634 666 742 774 2188 2218 2220 2250 634
Size: 32 76 32 1414 30 2 30 2730
Fragments arranged by size:
2730 1414 76 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1385 1385
Size: 4346
HindIII A'AGCT_T
Cuts at: 186 706 186
Size: 520 3826
KasI G'GCGC_C
Cuts at: 519 1776 2024 519
Size: 1257 248 2841
Fragments arranged by size:
2841 1257 248
KpnI G_GTAC'C
Cuts at: 1366 1366
Size: 4346
MfeI C'AATT_G
Cuts at: 140 140
Size: 4346
MluI A'CGCG_T
Cuts at: 3779 3779
Size: 4346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3017 3201 3017
Size: 184 4162
MscI TGG'CCA
Cuts at: 2107 2107
Size: 4346
MslI CAynn'nnrTG
Cuts at: 2335 2462 2335
Size: 127 4219
MspA1I CmG'CkG
Cuts at: 616 1457 1771 2131 3144 3389 616
Size: 841 314 360 1013 245 1573
Fragments arranged by size:
1573 1013 841 360 314 245
NaeI GCC'GGC
Cuts at: 2527 2527
Size: 4346
NarI GG'CG_CC
Cuts at: 520 1777 2025 520
Size: 1257 248 2841
Fragments arranged by size:
2841 1257 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4346
NgoMIV G'CCGG_C
Cuts at: 2525 2525
Size: 4346
NspI r_CATG'y
Cuts at: 796 1399 1899 2806 796
Size: 603 500 907 2336
Fragments arranged by size:
2336 907 603 500
PasI CC'CwG_GG
Cuts at: 514 514
Size: 4346
PciI A'CATG_T
Cuts at: 2802 2802
Size: 4346
PflMI CCAn_nnn'nTGG
Cuts at: 220 220
Size: 4346
PmeI GTTT'AAAC
Cuts at: 1345 1345
Size: 4346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 3485
Size: 32 4314
PshAI GACnn'nnGTC
Cuts at: 839 839
Size: 4346
PspOMI G'GGCC_C
Cuts at: 900 900
Size: 4346
PstI C_TGCA'G
Cuts at: 132 422 1393 2078 132
Size: 290 971 685 2400
Fragments arranged by size:
2400 971 685 290
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4346
PvuII CAG'CTG
Cuts at: 1771 2131 1771
Size: 360 3986
RsrII CG'GwC_CG
Cuts at: 2541 2541
Size: 4346
SacI G_AGCT'C
Cuts at: 1360 1360
Size: 4346
SalI G'TCGA_C
Cuts at: 1383 1383
Size: 4346
SapI GCTCTTCn'nnn_
Cuts at: 2369 2579 2369
Size: 210 4136
SbfI CC_TGCA'GG
Cuts at: 1393 1393
Size: 4346
ScaI AGT'ACT
Cuts at: 3863 3863
Size: 4346
SfcI C'TryA_G
Cuts at: 33 128 418 1389 2074 3067 3258 33
Size: 95 290 971 685 993 191 1121
Fragments arranged by size:
1121 993 971 685 290 191 95
SfoI GGC'GCC
Cuts at: 521 1778 2026 521
Size: 1257 248 2841
Fragments arranged by size:
2841 1257 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
SmaI CCC'GGG
Cuts at: 1368 1368
Size: 4346
SmlI C'TyrA_G
Cuts at: 736 1501 2416 2908 3170 736
Size: 765 915 492 262 1912
Fragments arranged by size:
1912 915 765 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4346
SphI G_CATG'C
Cuts at: 796 1399 1899 796
Size: 603 500 3243
Fragments arranged by size:
3243 603 500
SspI AAT'ATT
Cuts at: 954 954
Size: 4346
StuI AGG'CCT
Cuts at: 598 598
Size: 4346
StyI C'CwwG_G
Cuts at: 107 1480 1834 107
Size: 1373 354 2619
Fragments arranged by size:
2619 1373 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1929 2062 2521 2730 1929
Size: 133 459 209 3545
Fragments arranged by size:
3545 459 209 133
TatI w'GTAC_w
Cuts at: 3861 3861
Size: 4346
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 354 386 3863 3895 354
Size: 32 3477 32 805
Fragments arranged by size:
3477 805 32 32
Tth111I GACn'n_nGTC
Cuts at: 2143 2143
Size: 4346
XbaI T'CTAG_A
Cuts at: 1377 1377
Size: 4346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 711 1841 711
Size: 1130 3216
XhoI C'TCGA_G
Cuts at: 736 736
Size: 4346
XmaI C'CCGG_G
Cuts at: 1366 1366
Size: 4346
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsrGI BssHII BstEII BstXI
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HpaI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PpuMI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XmnI ZraI