Restriction map for
pF1KE0930
(Circular) (Six-base) MAPSORT of: pF1KE0930.seq Check: 8420 from: 1 to: 4268
pF1KE0930 4268 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1306 1306
Size: 4268
Acc65I G'GTAC_C
Cuts at: 1284 1284
Size: 4268
AclI AA'CG_TT
Cuts at: 4108 4108
Size: 4268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2093 2525 3272 3540 2093
Size: 432 747 268 2821
Fragments arranged by size:
2821 747 432 268
AflIII A'CryG_T
Cuts at: 1620 2724 3701 1620
Size: 1104 977 2187
Fragments arranged by size:
2187 1104 977
AgeI A'CCGG_T
Cuts at: 2656 2656
Size: 4268
AlwNI CAG_nnn'CTG
Cuts at: 582 1137 1573 3140 582
Size: 555 436 1567 1710
Fragments arranged by size:
1710 1567 555 436
ApaI G_GGCC'C
Cuts at: 633 718 633
Size: 85 4183
ApaLI G'TGCA_C
Cuts at: 3038 3038
Size: 4268
ApoI r'AATT_y
Cuts at: 253 1041 1272 3621 3877 253
Size: 788 231 2349 256 644
Fragments arranged by size:
2349 788 644 256 231
AseI AT'TA_AT
Cuts at: 20 20
Size: 4268
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
AvaI C'yCGr_G
Cuts at: 1190 1288 1190
Size: 98 4170
BamHI G'GATC_C
Cuts at: 1293 1293
Size: 4268
BanI G'GyrC_C
Cuts at: 945 1060 1284 1698 1946 1981 945
Size: 115 224 414 248 35 3232
Fragments arranged by size:
3232 414 248 224 115 35
BanII G_rGCy'C
Cuts at: 633 718 1282 2312 633
Size: 85 564 1030 2589
Fragments arranged by size:
2589 1030 564 85
BbeI G_GCGC'C
Cuts at: 1702 1950 1702
Size: 248 4020
BciVI GTATCCnnnnn_n'
Cuts at: 2166 2927 4268 2166
Size: 761 1341 2166
Fragments arranged by size:
2166 1341 761
BclI T'GATC_A
Cuts at: 450 1788 450
Size: 1338 2930
BglII A'GATC_T
Cuts at: 1783 3820 1783
Size: 2037 2231
BlpI GC'TnA_GC
Cuts at: 1380 1380
Size: 4268
Bme1580I G_kGCm'C
Cuts at: 633 718 1893 1986 3042 633
Size: 85 1175 93 1056 1859
Fragments arranged by size:
1859 1175 1056 93 85
BmrI ACTGGGnnnn_n'
Cuts at: 1068 1136 1480 1561 1896 4040 1068
Size: 68 344 81 335 2144 1296
Fragments arranged by size:
2144 1296 344 335 81 68
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1266 2635 1266
Size: 1369 2899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 783 916 937 604
Size: 179 133 21 3935
Fragments arranged by size:
3935 179 133 21
Bpu10I CC'TnA_GC
Cuts at: 566 1119 566
Size: 553 3715
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 339 1444 2323 2815 3113 339
Size: 1105 879 492 298 1494
Fragments arranged by size:
1494 1105 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1192 3972 1192
Size: 2780 1488
BsaAI yAC'GTr
Cuts at: 1621 1621
Size: 4268
BsaBI GATnn'nnATC
Cuts at: 6 1782 3819 6
Size: 1776 2037 455
Fragments arranged by size:
2037 1776 455
BsaHI Gr'CG_yC
Cuts at: 1699 1947 2649 4145 1699
Size: 248 702 1496 1822
Fragments arranged by size:
1822 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1457 1978 2656 2930 3077 3488 1457
Size: 521 678 274 147 411 2237
Fragments arranged by size:
2237 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1242 1255 1242
Size: 13 4255
BseYI C'CCAG_C
Cuts at: 221 632 1693 2451 3028 3524 221
Size: 411 1061 758 577 496 965
Fragments arranged by size:
1061 965 758 577 496 411
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 161
Size: 4268
BsiEI CG_ry'CG
Cuts at: 104 1810 1856 3064 104
Size: 1706 46 1208 1308
Fragments arranged by size:
1706 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1282 2060 2250 3042 1282
Size: 778 190 792 2508
Fragments arranged by size:
2508 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 633 718 1282 1893 1986 2060 2250 2312 3042
Size: 85 564 611 93 74 190 62 730
Cuts at: 3042 633
Size: 1859
Fragments arranged by size:
1859 730 611 564 190 93 85 74
62
BspEI T'CCGG_A
Cuts at: 3488 3488
Size: 4268
BspHI T'CATG_A
Cuts at: 3444 3444
Size: 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1318 1532 1834 2215 1318
Size: 214 302 381 3371
Fragments arranged by size:
3371 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2560 2614 4098 4263 50
Size: 2510 54 1484 165 55
Fragments arranged by size:
2510 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 191 509 2180 191
Size: 318 1671 2279
Fragments arranged by size:
2279 1671 318
BsrFI r'CCGG_y
Cuts at: 720 2266 2447 2656 720
Size: 1546 181 209 2332
Fragments arranged by size:
2332 1546 209 181
BssSI C'ACGA_G
Cuts at: 2539 2897 2539
Size: 358 3910
BstAPI GCAn_nnn'nTGC
Cuts at: 1645 1645
Size: 4268
BstBI TT'CG_AA
Cuts at: 2629 2629
Size: 4268
BstYI r'GATC_y
Cuts at: 1 845 921 1293 1760 1783 2118 2364 3376
Size: 844 76 372 467 23 335 246 1012
Cuts at: 3376 3462 3474 3820 1
Size: 86 12 346 449
Fragments arranged by size:
1012 844 467 449 372 346 335 246
86 76 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2218 2399 2562 2218
Size: 181 163 3924
Fragments arranged by size:
3924 181 163
BtsI GCAGTG_nn'
Cuts at: 1170 1176 1511 1170
Size: 6 335 3927
Fragments arranged by size:
3927 335 6
DraI TTT'AAA
Cuts at: 488 1267 488
Size: 779 3489
DrdI GACnn_nn'nnGTC
Cuts at: 1974 2832 1974
Size: 858 3410
EaeI y'GGCC_r
Cuts at: 1853 2027 2418 2445 3790 1853
Size: 174 391 27 1345 2331
Fragments arranged by size:
2331 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1853 1853
Size: 4268
EarI CTCTTCn'nnn_
Cuts at: 465 2291 2501 465
Size: 1826 210 2232
Fragments arranged by size:
2232 1826 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 618 2476 2786 2932 4078 618
Size: 1858 310 146 1146 808
Fragments arranged by size:
1858 1146 808 310 146
EcoICRI GAG'CTC
Cuts at: 1280 1280
Size: 4268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 783 916 937 2093 2525 3272 3540 604
Size: 179 133 21 1156 432 747 268 1332
Fragments arranged by size:
1332 1156 747 432 268 179 133 21
EcoO109I rG'GnC_Cy
Cuts at: 225 714 988 1407 225
Size: 489 274 419 3086
Fragments arranged by size:
3086 489 419 274
EcoRI G'AATT_C
Cuts at: 1272 1272
Size: 4268
EcoRV GAT'ATC
Cuts at: 978 978
Size: 4268
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 4268
FspI TGC'GCA
Cuts at: 2049 2049
Size: 4268
HaeII r_GCGC'y
Cuts at: 1702 1950 2972 3951 1702
Size: 248 1022 979 2019
Fragments arranged by size:
2019 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 147 179 385 417 1237 1269 2110 2140 2142
Size: 32 206 32 820 32 841 30 2
Cuts at: 2142 2172 147
Size: 30 2243
Fragments arranged by size:
2243 841 820 206 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1307 1307
Size: 4268
KasI G'GCGC_C
Cuts at: 1698 1946 1698
Size: 248 4020
KpnI G_GTAC'C
Cuts at: 1288 1288
Size: 4268
MluI A'CGCG_T
Cuts at: 3701 3701
Size: 4268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2939 3123 2939
Size: 184 4084
MscI TGG'CCA
Cuts at: 2029 2029
Size: 4268
MslI CAynn'nnrTG
Cuts at: 2257 2384 2257
Size: 127 4141
MspA1I CmG'CkG
Cuts at: 636 744 1379 1693 2053 3066 3311 636
Size: 108 635 314 360 1013 245 1593
Fragments arranged by size:
1593 1013 635 360 314 245 108
NaeI GCC'GGC
Cuts at: 722 2449 722
Size: 1727 2541
NarI GG'CG_CC
Cuts at: 1699 1947 1699
Size: 248 4020
NgoMIV G'CCGG_C
Cuts at: 720 2447 720
Size: 1727 2541
NspI r_CATG'y
Cuts at: 1321 1821 2728 1321
Size: 500 907 2861
Fragments arranged by size:
2861 907 500
PciI A'CATG_T
Cuts at: 2724 2724
Size: 4268
PmeI GTTT'AAAC
Cuts at: 1267 1267
Size: 4268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 3407
Size: 32 4236
PpuMI rG'GwC_Cy
Cuts at: 225 988 225
Size: 763 3505
PsiI TTA'TAA
Cuts at: 115 115
Size: 4268
PspOMI G'GGCC_C
Cuts at: 629 714 629
Size: 85 4183
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 635 667 635
Size: 32 4236
PstI C_TGCA'G
Cuts at: 1315 2000 1315
Size: 685 3583
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4268
PvuII CAG'CTG
Cuts at: 636 744 1693 2053 636
Size: 108 949 360 2851
Fragments arranged by size:
2851 949 360 108
RsrII CG'GwC_CG
Cuts at: 2463 2463
Size: 4268
SacI G_AGCT'C
Cuts at: 1282 1282
Size: 4268
SalI G'TCGA_C
Cuts at: 1305 1305
Size: 4268
SanDI GG'GwC_CC
Cuts at: 225 225
Size: 4268
SapI GCTCTTCn'nnn_
Cuts at: 2291 2501 2291
Size: 210 4058
SbfI CC_TGCA'GG
Cuts at: 1315 1315
Size: 4268
ScaI AGT'ACT
Cuts at: 866 3785 866
Size: 2919 1349
SexAI A'CCwGG_T
Cuts at: 674 674
Size: 4268
SfcI C'TryA_G
Cuts at: 33 652 1311 1996 2989 3180 33
Size: 619 659 685 993 191 1121
Fragments arranged by size:
1121 993 685 659 619 191
SfoI GGC'GCC
Cuts at: 1700 1948 1700
Size: 248 4020
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
SmaI CCC'GGG
Cuts at: 1290 1290
Size: 4268
SmlI C'TyrA_G
Cuts at: 318 1423 2338 2830 3092 318
Size: 1105 915 492 262 1494
Fragments arranged by size:
1494 1105 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4268
SphI G_CATG'C
Cuts at: 1321 1821 1321
Size: 500 3768
StyI C'CwwG_G
Cuts at: 1089 1402 1756 1089
Size: 313 354 3601
Fragments arranged by size:
3601 354 313
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1851 1984 2443 2652 1851
Size: 133 459 209 3467
Fragments arranged by size:
3467 459 209 133
TatI w'GTAC_w
Cuts at: 864 3783 864
Size: 2919 1349
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3785 3817 3785
Size: 32 4236
Tth111I GACn'n_nGTC
Cuts at: 2065 2065
Size: 4268
XbaI T'CTAG_A
Cuts at: 1299 1299
Size: 4268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1763 1763
Size: 4268
XmaI C'CCGG_G
Cuts at: 1288 1288
Size: 4268
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PpuMI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PspXI SacII SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI