Restriction map for
pF1KE0927
(Circular) (Six-base) MAPSORT of: pF1KE0927.seq Check: 5955 from: 1 to: 4202
pF1KE0927 4202 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 431 431
Size: 4202
AccI GT'mk_AC
Cuts at: 1240 1240
Size: 4202
Acc65I G'GTAC_C
Cuts at: 1218 1218
Size: 4202
AclI AA'CG_TT
Cuts at: 4042 4042
Size: 4202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 1082 1157 2027 2459 3206 3474 488
Size: 594 75 870 432 747 268 1216
Fragments arranged by size:
1216 870 747 594 432 268 75
AflIII A'CryG_T
Cuts at: 696 1554 2658 3635 696
Size: 858 1104 977 1263
Fragments arranged by size:
1263 1104 977 858
AgeI A'CCGG_T
Cuts at: 2590 2590
Size: 4202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 703 703
Size: 4202
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 137 169 137
Size: 32 4170
AlwNI CAG_nnn'CTG
Cuts at: 516 1180 1507 3074 516
Size: 664 327 1567 1644
Fragments arranged by size:
1644 1567 664 327
ApaLI G'TGCA_C
Cuts at: 2972 2972
Size: 4202
ApoI r'AATT_y
Cuts at: 1206 3555 3811 1206
Size: 2349 256 1597
Fragments arranged by size:
2349 1597 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4202
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4202
AvaI C'yCGr_G
Cuts at: 1222 1222
Size: 4202
BamHI G'GATC_C
Cuts at: 166 1227 166
Size: 1061 3141
BanI G'GyrC_C
Cuts at: 916 1218 1632 1880 1915 916
Size: 302 414 248 35 3203
Fragments arranged by size:
3203 414 302 248 35
BanII G_rGCy'C
Cuts at: 766 1216 2246 766
Size: 450 1030 2722
Fragments arranged by size:
2722 1030 450
BbeI G_GCGC'C
Cuts at: 1636 1884 1636
Size: 248 3954
BbsI GAAGACnn'nnnn_
Cuts at: 845 845
Size: 4202
BciVI GTATCCnnnnn_n'
Cuts at: 2100 2861 4202 2100
Size: 761 1341 2100
Fragments arranged by size:
2100 1341 761
BclI T'GATC_A
Cuts at: 1722 1722
Size: 4202
BglII A'GATC_T
Cuts at: 887 1717 3754 887
Size: 830 2037 1335
Fragments arranged by size:
2037 1335 830
BlpI GC'TnA_GC
Cuts at: 1314 1314
Size: 4202
Bme1580I G_kGCm'C
Cuts at: 1827 1920 2976 1827
Size: 93 1056 3053
Fragments arranged by size:
3053 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1414 1495 1830 3974 1414
Size: 81 335 2144 1642
Fragments arranged by size:
2144 1642 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 780 2569 780
Size: 1789 2413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 677 677
Size: 4202
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 1378 2257 2749 3047 784
Size: 594 879 492 298 1939
Fragments arranged by size:
1939 879 594 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 658 3906 658
Size: 3248 954
BsaAI yAC'GTr
Cuts at: 1555 1555
Size: 4202
BsaBI GATnn'nnATC
Cuts at: 6 1716 3753 6
Size: 1710 2037 455
Fragments arranged by size:
2037 1710 455
BsaHI Gr'CG_yC
Cuts at: 118 1633 1881 2583 4079 118
Size: 1515 248 702 1496 241
Fragments arranged by size:
1515 1496 702 248 241
BsaWI w'CCGG_w
Cuts at: 1391 1912 2590 2864 3011 3422 1391
Size: 521 678 274 147 411 2171
Fragments arranged by size:
2171 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 394 424 394
Size: 30 4172
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 219 1081 219
Size: 862 3340
BseYI C'CCAG_C
Cuts at: 222 813 1627 2385 2962 3458 222
Size: 591 814 758 577 496 966
Fragments arranged by size:
966 814 758 591 577 496
BsiEI CG_ry'CG
Cuts at: 104 770 1744 1790 2998 104
Size: 666 974 46 1208 1308
Fragments arranged by size:
1308 1208 974 666 46
BsiHKAI G_wGCw'C
Cuts at: 1216 1994 2184 2976 1216
Size: 778 190 792 2442
Fragments arranged by size:
2442 792 778 190
BsmI GAATG_Cn'
Cuts at: 731 731
Size: 4202
Bsp1286I G_dGCh'C
Cuts at: 766 1216 1827 1920 1994 2184 2246 2976 766
Size: 450 611 93 74 190 62 730 1992
Fragments arranged by size:
1992 730 611 450 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3422 3422
Size: 4202
BspHI T'CATG_A
Cuts at: 3378 3378
Size: 4202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 431 1151 1252 1466 1768 2149 431
Size: 720 101 214 302 381 2484
Fragments arranged by size:
2484 720 381 302 214 101
BsrBI CCG'CTC
Cuts at: 50 273 728 810 999 2494 2548 4032 4197
Size: 223 455 82 189 1495 54 1484 165
Cuts at: 4197 50
Size: 55
Fragments arranged by size:
1495 1484 455 223 189 165 82 55
54
BsrDI GCAATG_nn'
Cuts at: 2114 2114
Size: 4202
BsrFI r'CCGG_y
Cuts at: 2200 2381 2590 2200
Size: 181 209 3812
Fragments arranged by size:
3812 209 181
BssSI C'ACGA_G
Cuts at: 2473 2831 2473
Size: 358 3844
BstAPI GCAn_nnn'nTGC
Cuts at: 1579 1579
Size: 4202
BstBI TT'CG_AA
Cuts at: 2563 2563
Size: 4202
BstXI CCAn_nnnn'nTGG
Cuts at: 455 603 651 455
Size: 148 48 4006
Fragments arranged by size:
4006 148 48
BstYI r'GATC_y
Cuts at: 1 166 887 1227 1694 1717 2052 2298 3310
Size: 165 721 340 467 23 335 246 1012
Cuts at: 3310 3396 3408 3754 1
Size: 86 12 346 449
Fragments arranged by size:
1012 721 467 449 346 340 335 246
165 86 23 12
BtgI C'CryG_G
Cuts at: 107 635 107
Size: 528 3674
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2152 2333 2496 2152
Size: 181 163 3858
Fragments arranged by size:
3858 181 163
BtsI GCAGTG_nn'
Cuts at: 1445 1445
Size: 4202
DraI TTT'AAA
Cuts at: 1074 1201 1074
Size: 127 4075
DrdI GACnn_nn'nnGTC
Cuts at: 541 1908 2766 541
Size: 1367 858 1977
Fragments arranged by size:
1977 1367 858
EaeI y'GGCC_r
Cuts at: 293 428 767 1094 1787 1961 2352 2379 3724
Size: 135 339 327 693 174 391 27 1345
Cuts at: 3724 293
Size: 771
Fragments arranged by size:
1345 771 693 391 339 327 174 135
27
EagI C'GGCC_G
Cuts at: 767 1787 767
Size: 1020 3182
EarI CTCTTCn'nnn_
Cuts at: 718 1058 2225 2435 718
Size: 340 1167 210 2485
Fragments arranged by size:
2485 1167 340 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2410 2720 2866 4012 2410
Size: 310 146 1146 2600
Fragments arranged by size:
2600 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1214 1214
Size: 4202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 677 1082 1157 2027 2459 3206 3474 488
Size: 189 405 75 870 432 747 268 1216
Fragments arranged by size:
1216 870 747 432 405 268 189 75
EcoNI CCTnn'n_nnAGG
Cuts at: 520 520
Size: 4202
EcoO109I rG'GnC_Cy
Cuts at: 474 513 846 1341 474
Size: 39 333 495 3335
Fragments arranged by size:
3335 495 333 39
EcoRI G'AATT_C
Cuts at: 1206 1206
Size: 4202
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 4202
FspI TGC'GCA
Cuts at: 1983 1983
Size: 4202
HaeII r_GCGC'y
Cuts at: 1636 1884 2906 3885 1636
Size: 248 1022 979 1953
Fragments arranged by size:
1953 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2044 2074 2076 2106 2044
Size: 30 2 30 4140
Fragments arranged by size:
4140 30 30 2
HincII GTy'rAC
Cuts at: 1241 1241
Size: 4202
KasI G'GCGC_C
Cuts at: 1632 1880 1632
Size: 248 3954
KpnI G_GTAC'C
Cuts at: 1222 1222
Size: 4202
MluI A'CGCG_T
Cuts at: 3635 3635
Size: 4202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2873 3057 2873
Size: 184 4018
MscI TGG'CCA
Cuts at: 295 430 1963 295
Size: 135 1533 2534
Fragments arranged by size:
2534 1533 135
MslI CAynn'nnrTG
Cuts at: 259 2191 2318 259
Size: 1932 127 2143
Fragments arranged by size:
2143 1932 127
MspA1I CmG'CkG
Cuts at: 1313 1627 1987 3000 3245 1313
Size: 314 360 1013 245 2270
Fragments arranged by size:
2270 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2383 2383
Size: 4202
NarI GG'CG_CC
Cuts at: 1633 1881 1633
Size: 248 3954
NcoI C'CATG_G
Cuts at: 107 107
Size: 4202
NgoMIV G'CCGG_C
Cuts at: 2381 2381
Size: 4202
NspI r_CATG'y
Cuts at: 154 315 573 700 879 943 1255 1755 2662
Size: 161 258 127 179 64 312 500 907
Cuts at: 2662 154
Size: 1694
Fragments arranged by size:
1694 907 500 312 258 179 161 127
64
PciI A'CATG_T
Cuts at: 696 2658 696
Size: 1962 2240
PflMI CCAn_nnn'nTGG
Cuts at: 834 834
Size: 4202
PmeI GTTT'AAAC
Cuts at: 1201 1201
Size: 4202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3341 3373 3341
Size: 32 4170
PpuMI rG'GwC_Cy
Cuts at: 474 474
Size: 4202
PsiI TTA'TAA
Cuts at: 854 854
Size: 4202
PstI C_TGCA'G
Cuts at: 521 1185 1249 1934 521
Size: 664 64 685 2789
Fragments arranged by size:
2789 685 664 64
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4202
PvuII CAG'CTG
Cuts at: 1627 1987 1627
Size: 360 3842
RsrII CG'GwC_CG
Cuts at: 2397 2397
Size: 4202
SacI G_AGCT'C
Cuts at: 1216 1216
Size: 4202
SalI G'TCGA_C
Cuts at: 1239 1239
Size: 4202
SapI GCTCTTCn'nnn_
Cuts at: 718 2225 2435 718
Size: 1507 210 2485
Fragments arranged by size:
2485 1507 210
SbfI CC_TGCA'GG
Cuts at: 521 1249 521
Size: 728 3474
ScaI AGT'ACT
Cuts at: 3719 3719
Size: 4202
SfcI C'TryA_G
Cuts at: 33 517 1181 1245 1930 2923 3114 33
Size: 484 664 64 685 993 191 1121
Fragments arranged by size:
1121 993 685 664 484 191 64
SfoI GGC'GCC
Cuts at: 1634 1882 1634
Size: 248 3954
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4202
SmaI CCC'GGG
Cuts at: 1224 1224
Size: 4202
SmlI C'TyrA_G
Cuts at: 799 1357 2272 2764 3026 799
Size: 558 915 492 262 1975
Fragments arranged by size:
1975 915 558 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4202
SphI G_CATG'C
Cuts at: 573 943 1255 1755 573
Size: 370 312 500 3020
Fragments arranged by size:
3020 500 370 312
StuI AGG'CCT
Cuts at: 706 706
Size: 4202
StyI C'CwwG_G
Cuts at: 107 596 1336 1690 107
Size: 489 740 354 2619
Fragments arranged by size:
2619 740 489 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1785 1918 2377 2586 1785
Size: 133 459 209 3401
Fragments arranged by size:
3401 459 209 133
TatI w'GTAC_w
Cuts at: 3717 3717
Size: 4202
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3719 3751 3719
Size: 32 4170
Tth111I GACn'n_nGTC
Cuts at: 1999 1999
Size: 4202
XbaI T'CTAG_A
Cuts at: 1233 1233
Size: 4202
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 387 438 1047 1697 387
Size: 51 609 650 2892
Fragments arranged by size:
2892 650 609 51
XmaI C'CCGG_G
Cuts at: 1222 1222
Size: 4202
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI