Restriction map for
pF1KE0926
(Circular) (Six-base) MAPSORT of: pF1KE0926.seq Check: 4214 from: 1 to: 4190
pF1KE0926 4190 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 673 1228 673
Size: 555 3635
Acc65I G'GTAC_C
Cuts at: 1206 1206
Size: 4190
AclI AA'CG_TT
Cuts at: 4030 4030
Size: 4190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 652 2015 2447 3194 3462 485
Size: 167 1363 432 747 268 1213
Fragments arranged by size:
1363 1213 747 432 268 167
AflIII A'CryG_T
Cuts at: 579 1542 2646 3623 579
Size: 963 1104 977 1146
Fragments arranged by size:
1146 1104 977 963
AgeI A'CCGG_T
Cuts at: 2578 2578
Size: 4190
AhdI GACnn_n'nnGTC
Cuts at: 1152 1152
Size: 4190
AleI CACnn'nnGTG
Cuts at: 932 932
Size: 4190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 209 209
Size: 4190
AlwNI CAG_nnn'CTG
Cuts at: 535 660 846 1495 3062 535
Size: 125 186 649 1567 1663
Fragments arranged by size:
1663 1567 649 186 125
ApaI G_GGCC'C
Cuts at: 700 700
Size: 4190
ApaLI G'TGCA_C
Cuts at: 1113 2960 1113
Size: 1847 2343
ApoI r'AATT_y
Cuts at: 788 1048 1194 3543 3799 788
Size: 260 146 2349 256 1179
Fragments arranged by size:
2349 1179 260 256 146
AseI AT'TA_AT
Cuts at: 20 20
Size: 4190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
AvaI C'yCGr_G
Cuts at: 1210 1210
Size: 4190
BamHI G'GATC_C
Cuts at: 826 1215 826
Size: 389 3801
BanI G'GyrC_C
Cuts at: 1206 1620 1868 1903 1206
Size: 414 248 35 3493
Fragments arranged by size:
3493 414 248 35
BanII G_rGCy'C
Cuts at: 317 700 1070 1204 2234 317
Size: 383 370 134 1030 2273
Fragments arranged by size:
2273 1030 383 370 134
BbeI G_GCGC'C
Cuts at: 1624 1872 1624
Size: 248 3942
BbsI GAAGACnn'nnnn_
Cuts at: 443 640 924 443
Size: 197 284 3709
Fragments arranged by size:
3709 284 197
BbvCI CC'TCA_GC
Cuts at: 831 831
Size: 4190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1150 1184 1150
Size: 34 4156
BciVI GTATCCnnnnn_n'
Cuts at: 2088 2849 4190 2088
Size: 761 1341 2088
Fragments arranged by size:
2088 1341 761
BclI T'GATC_A
Cuts at: 1710 1710
Size: 4190
BglII A'GATC_T
Cuts at: 572 1705 3742 572
Size: 1133 2037 1020
Fragments arranged by size:
2037 1133 1020
BlpI GC'TnA_GC
Cuts at: 1302 1302
Size: 4190
Bme1580I G_kGCm'C
Cuts at: 700 1117 1815 1908 2964 700
Size: 417 698 93 1056 1926
Fragments arranged by size:
1926 1056 698 417 93
BmgBI CAC'GTC
Cuts at: 479 479
Size: 4190
BmrI ACTGGGnnnn_n'
Cuts at: 637 1402 1483 1818 3962 637
Size: 765 81 335 2144 865
Fragments arranged by size:
2144 865 765 335 81
BmtI G_CTAG'C
Cuts at: 413 413
Size: 4190
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 4190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 385
Size: 4190
Bpu10I CC'TnA_GC
Cuts at: 831 831
Size: 4190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 2245 2737 3035 1366
Size: 879 492 298 2521
Fragments arranged by size:
2521 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3894 3894
Size: 4190
BsaAI yAC'GTr
Cuts at: 1543 1543
Size: 4190
BsaBI GATnn'nnATC
Cuts at: 6 1704 3741 6
Size: 1698 2037 455
Fragments arranged by size:
2037 1698 455
BsaHI Gr'CG_yC
Cuts at: 1621 1869 2571 4067 1621
Size: 248 702 1496 1744
Fragments arranged by size:
1744 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1379 1900 2578 2852 2999 3410 1379
Size: 521 678 274 147 411 2159
Fragments arranged by size:
2159 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 754 784 754
Size: 30 4160
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 386 537 743 820 386
Size: 151 206 77 3756
Fragments arranged by size:
3756 206 151 77
BseYI C'CCAG_C
Cuts at: 285 1615 2373 2950 3446 285
Size: 1330 758 577 496 1029
Fragments arranged by size:
1330 1029 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 673
Size: 4190
BsiEI CG_ry'CG
Cuts at: 104 1732 1778 2986 104
Size: 1628 46 1208 1308
Fragments arranged by size:
1628 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 499 559 1070 1117 1204 1982 2172 2964 499
Size: 60 511 47 87 778 190 792 1725
Fragments arranged by size:
1725 792 778 511 190 87 60 47
Bsp1286I G_dGCh'C
Cuts at: 317 499 559 700 1070 1117 1204 1815 1908
Size: 182 60 141 370 47 87 611 93
Cuts at: 1908 1982 2172 2234 2964 317
Size: 74 190 62 730 1543
Fragments arranged by size:
1543 730 611 370 190 182 141 93
87 74 62 60 47
BspEI T'CCGG_A
Cuts at: 3410 3410
Size: 4190
BspHI T'CATG_A
Cuts at: 3366 3366
Size: 4190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 165 177 1240 1454 1756 2137 165
Size: 12 1063 214 302 381 2218
Fragments arranged by size:
2218 1063 381 302 214 12
BsrBI CCG'CTC
Cuts at: 50 2482 2536 4020 4185 50
Size: 2432 54 1484 165 55
Fragments arranged by size:
2432 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 881 2102 881
Size: 1221 2969
BsrFI r'CCGG_y
Cuts at: 2188 2369 2578 2188
Size: 181 209 3800
Fragments arranged by size:
3800 209 181
BsrGI T'GTAC_A
Cuts at: 959 959
Size: 4190
BssSI C'ACGA_G
Cuts at: 2461 2819 2461
Size: 358 3832
BstAPI GCAn_nnn'nTGC
Cuts at: 660 846 1121 1567 660
Size: 186 275 446 3283
Fragments arranged by size:
3283 446 275 186
BstBI TT'CG_AA
Cuts at: 2551 2551
Size: 4190
BstXI CCAn_nnnn'nTGG
Cuts at: 276 645 276
Size: 369 3821
BstYI r'GATC_y
Cuts at: 1 213 572 826 1215 1682 1705 2040 2286
Size: 212 359 254 389 467 23 335 246
Cuts at: 2286 3298 3384 3396 3742 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 389 359 346 335 254
246 212 86 23 12
BstZ17I GTA'TAC
Cuts at: 674 674
Size: 4190
BtgI C'CryG_G
Cuts at: 107 275 665 1181 107
Size: 168 390 516 3116
Fragments arranged by size:
3116 516 390 168
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1147 2140 2321 2484 1147
Size: 993 181 163 2853
Fragments arranged by size:
2853 993 181 163
BtsI GCAGTG_nn'
Cuts at: 191 1433 191
Size: 1242 2948
DraI TTT'AAA
Cuts at: 1189 1189
Size: 4190
DrdI GACnn_nn'nnGTC
Cuts at: 1896 2754 1896
Size: 858 3332
EaeI y'GGCC_r
Cuts at: 1775 1949 2340 2367 3712 1775
Size: 174 391 27 1345 2253
Fragments arranged by size:
2253 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1775 1775
Size: 4190
EarI CTCTTCn'nnn_
Cuts at: 890 907 2213 2423 890
Size: 17 1306 210 2657
Fragments arranged by size:
2657 1306 210 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2398 2708 2854 4000 2398
Size: 310 146 1146 2588
Fragments arranged by size:
2588 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1068 1202 1068
Size: 134 4056
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 485 652 2015 2447 3194 3462 385
Size: 100 167 1363 432 747 268 1113
Fragments arranged by size:
1363 1113 747 432 268 167 100
EcoO109I rG'GnC_Cy
Cuts at: 696 697 1329 696
Size: 1 632 3557
Fragments arranged by size:
3557 632 1
EcoRI G'AATT_C
Cuts at: 1194 1194
Size: 4190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 919 2209 919
Size: 1290 2900
FspI TGC'GCA
Cuts at: 1971 1971
Size: 4190
HaeII r_GCGC'y
Cuts at: 1624 1872 2894 3873 1624
Size: 248 1022 979 1941
Fragments arranged by size:
1941 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 534 566 754 786 811 843 1024 1056 1138
Size: 32 188 32 25 32 181 32 82
Cuts at: 1138 1170 2032 2062 2064 2094 534
Size: 32 862 30 2 30 2630
Fragments arranged by size:
2630 862 188 181 82 32 32 32
32 32 30 30 25 2
HincII GTy'rAC
Cuts at: 369 482 762 1229 369
Size: 113 280 467 3330
Fragments arranged by size:
3330 467 280 113
KasI G'GCGC_C
Cuts at: 1620 1868 1620
Size: 248 3942
KpnI G_GTAC'C
Cuts at: 1210 1210
Size: 4190
MfeI C'AATT_G
Cuts at: 815 815
Size: 4190
MluI A'CGCG_T
Cuts at: 3623 3623
Size: 4190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 171 277 2861 3045 171
Size: 106 2584 184 1316
Fragments arranged by size:
2584 1316 184 106
MscI TGG'CCA
Cuts at: 1951 1951
Size: 4190
MslI CAynn'nnrTG
Cuts at: 643 772 932 2179 2306 643
Size: 129 160 1247 127 2527
Fragments arranged by size:
2527 1247 160 129 127
MspA1I CmG'CkG
Cuts at: 1301 1615 1975 2988 3233 1301
Size: 314 360 1013 245 2258
Fragments arranged by size:
2258 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2371 2371
Size: 4190
NarI GG'CG_CC
Cuts at: 1621 1869 1621
Size: 248 3942
NcoI C'CATG_G
Cuts at: 107 275 665 1181 107
Size: 168 390 516 3116
Fragments arranged by size:
3116 516 390 168
NgoMIV G'CCGG_C
Cuts at: 2369 2369
Size: 4190
NheI G'CTAG_C
Cuts at: 409 409
Size: 4190
NspI r_CATG'y
Cuts at: 417 583 1243 1743 2650 417
Size: 166 660 500 907 1957
Fragments arranged by size:
1957 907 660 500 166
PciI A'CATG_T
Cuts at: 579 2646 579
Size: 2067 2123
PflMI CCAn_nnn'nTGG
Cuts at: 724 724
Size: 4190
PfoI T'CCnGG_A
Cuts at: 260 1042 260
Size: 782 3408
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 4158
PsiI TTA'TAA
Cuts at: 1019 1019
Size: 4190
PspOMI G'GGCC_C
Cuts at: 696 696
Size: 4190
PstI C_TGCA'G
Cuts at: 690 705 851 1237 1922 690
Size: 15 146 386 685 2958
Fragments arranged by size:
2958 685 386 146 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4190
PvuII CAG'CTG
Cuts at: 1615 1975 1615
Size: 360 3830
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4190
SacI G_AGCT'C
Cuts at: 1070 1204 1070
Size: 134 4056
SalI G'TCGA_C
Cuts at: 1227 1227
Size: 4190
SapI GCTCTTCn'nnn_
Cuts at: 2213 2423 2213
Size: 210 3980
SbfI CC_TGCA'GG
Cuts at: 1237 1237
Size: 4190
ScaI AGT'ACT
Cuts at: 3707 3707
Size: 4190
SfcI C'TryA_G
Cuts at: 33 686 701 847 856 1233 1918 2911 3102
Size: 653 15 146 9 377 685 993 191
Cuts at: 3102 33
Size: 1121
Fragments arranged by size:
1121 993 685 653 377 191 146 15
9
SfoI GGC'GCC
Cuts at: 1622 1870 1622
Size: 248 3942
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
SmaI CCC'GGG
Cuts at: 1212 1212
Size: 4190
SmlI C'TyrA_G
Cuts at: 1345 2260 2752 3014 1345
Size: 915 492 262 2521
Fragments arranged by size:
2521 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4190
SphI G_CATG'C
Cuts at: 417 1243 1743 417
Size: 826 500 2864
Fragments arranged by size:
2864 826 500
StyI C'CwwG_G
Cuts at: 107 275 389 665 1181 1324 1678 107
Size: 168 114 276 516 143 354 2619
Fragments arranged by size:
2619 516 354 276 168 143 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 613 1773 1906 2365 2574 613
Size: 1160 133 459 209 2229
Fragments arranged by size:
2229 1160 459 209 133
TatI w'GTAC_w
Cuts at: 811 959 3705 811
Size: 148 2746 1296
Fragments arranged by size:
2746 1296 148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3707 3739 3707
Size: 32 4158
Tth111I GACn'n_nGTC
Cuts at: 1987 1987
Size: 4190
XbaI T'CTAG_A
Cuts at: 1221 1221
Size: 4190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 780 1685 114
Size: 666 905 2619
Fragments arranged by size:
2619 905 666
XmaI C'CCGG_G
Cuts at: 1210 1210
Size: 4190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PflMI PfoI PmeI
PpiI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI AscI AvrII BaeI
BfrBI BglI BsiWI BsmI BsmBI BssHII BstEII Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI NdeI NotI NruI NsiI PacI PasI PmlI
PpuMI PshAI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI