Restriction map for
pF1KE0925
(Circular) (Six-base) MAPSORT of: pF1KE0925.seq Check: 3155 from: 1 to: 4097
pF1KE0925 4097 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1135 1135
Size: 4097
Acc65I G'GTAC_C
Cuts at: 1113 1113
Size: 4097
AclI AA'CG_TT
Cuts at: 3937 3937
Size: 4097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1922 2354 3101 3369 1922
Size: 432 747 268 2650
Fragments arranged by size:
2650 747 432 268
AflIII A'CryG_T
Cuts at: 1449 2553 3530 1449
Size: 1104 977 2016
Fragments arranged by size:
2016 1104 977
AgeI A'CCGG_T
Cuts at: 2485 2485
Size: 4097
AlwNI CAG_nnn'CTG
Cuts at: 307 1402 2969 307
Size: 1095 1567 1435
Fragments arranged by size:
1567 1435 1095
ApaLI G'TGCA_C
Cuts at: 2867 2867
Size: 4097
ApoI r'AATT_y
Cuts at: 231 454 556 752 1101 3450 3706 231
Size: 223 102 196 349 2349 256 622
Fragments arranged by size:
2349 622 349 256 223 196 102
AseI AT'TA_AT
Cuts at: 20 359 20
Size: 339 3758
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 151 1117 151
Size: 966 3131
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 721 754 721
Size: 33 4064
BamHI G'GATC_C
Cuts at: 1122 1122
Size: 4097
BanI G'GyrC_C
Cuts at: 131 1113 1527 1775 1810 131
Size: 982 414 248 35 2418
Fragments arranged by size:
2418 982 414 248 35
BanII G_rGCy'C
Cuts at: 170 198 1111 2141 170
Size: 28 913 1030 2126
Fragments arranged by size:
2126 1030 913 28
BbeI G_GCGC'C
Cuts at: 1531 1779 1531
Size: 248 3849
BbsI GAAGACnn'nnnn_
Cuts at: 150 574 150
Size: 424 3673
BciVI GTATCCnnnnn_n'
Cuts at: 722 843 1995 2756 4097 722
Size: 121 1152 761 1341 722
Fragments arranged by size:
1341 1152 761 722 121
BclI T'GATC_A
Cuts at: 1617 1617
Size: 4097
BglII A'GATC_T
Cuts at: 1612 3649 1612
Size: 2037 2060
BlpI GC'TnA_GC
Cuts at: 347 1209 347
Size: 862 3235
Bme1580I G_kGCm'C
Cuts at: 1722 1815 2871 1722
Size: 93 1056 2948
Fragments arranged by size:
2948 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1309 1390 1725 3869 1309
Size: 81 335 2144 1537
Fragments arranged by size:
2144 1537 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4097
Bpu10I CC'TnA_GC
Cuts at: 1079 1079
Size: 4097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 1273 2152 2644 2942 362
Size: 911 879 492 298 1517
Fragments arranged by size:
1517 911 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3801 3801
Size: 4097
BsaAI yAC'GTr
Cuts at: 1450 1450
Size: 4097
BsaBI GATnn'nnATC
Cuts at: 6 854 1611 3648 6
Size: 848 757 2037 455
Fragments arranged by size:
2037 848 757 455
BsaHI Gr'CG_yC
Cuts at: 1528 1776 2478 3974 1528
Size: 248 702 1496 1651
Fragments arranged by size:
1651 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1286 1807 2485 2759 2906 3317 1286
Size: 521 678 274 147 411 2066
Fragments arranged by size:
2066 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 185 215 185
Size: 30 4067
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 206 206
Size: 4097
BseYI C'CCAG_C
Cuts at: 1522 2280 2857 3353 1522
Size: 758 577 496 2266
Fragments arranged by size:
2266 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 722 722
Size: 4097
BsiEI CG_ry'CG
Cuts at: 104 1639 1685 2893 104
Size: 1535 46 1208 1308
Fragments arranged by size:
1535 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 170 1111 1889 2079 2871 170
Size: 941 778 190 792 1396
Fragments arranged by size:
1396 941 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 170 198 1111 1722 1815 1889 2079 2141 2871
Size: 28 913 611 93 74 190 62 730
Cuts at: 2871 170
Size: 1396
Fragments arranged by size:
1396 913 730 611 190 93 74 62
28
BspEI T'CCGG_A
Cuts at: 3317 3317
Size: 4097
BspHI T'CATG_A
Cuts at: 3273 3273
Size: 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 342 1147 1361 1663 2044 342
Size: 805 214 302 381 2395
Fragments arranged by size:
2395 805 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2389 2443 3927 4092 50
Size: 2339 54 1484 165 55
Fragments arranged by size:
2339 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 123 2009 123
Size: 1886 2211
BsrFI r'CCGG_y
Cuts at: 2095 2276 2485 2095
Size: 181 209 3707
Fragments arranged by size:
3707 209 181
BssSI C'ACGA_G
Cuts at: 2368 2726 2368
Size: 358 3739
BstAPI GCAn_nnn'nTGC
Cuts at: 1474 1474
Size: 4097
BstBI TT'CG_AA
Cuts at: 2458 2458
Size: 4097
BstYI r'GATC_y
Cuts at: 1 1122 1589 1612 1947 2193 3205 3291 3303
Size: 1121 467 23 335 246 1012 86 12
Cuts at: 3303 3649 1
Size: 346 449
Fragments arranged by size:
1121 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 845 845
Size: 4097
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2047 2228 2391 2047
Size: 181 163 3753
Fragments arranged by size:
3753 181 163
BtsI GCAGTG_nn'
Cuts at: 1340 1340
Size: 4097
DraI TTT'AAA
Cuts at: 450 1096 450
Size: 646 3451
DrdI GACnn_nn'nnGTC
Cuts at: 154 1803 2661 154
Size: 1649 858 1590
Fragments arranged by size:
1649 1590 858
EaeI y'GGCC_r
Cuts at: 770 1682 1856 2247 2274 3619 770
Size: 912 174 391 27 1345 1248
Fragments arranged by size:
1345 1248 912 391 174 27
EagI C'GGCC_G
Cuts at: 1682 1682
Size: 4097
EarI CTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2305 2615 2761 3907 2305
Size: 310 146 1146 2495
Fragments arranged by size:
2495 1146 310 146
EcoICRI GAG'CTC
Cuts at: 168 1109 168
Size: 941 3156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1922 2354 3101 3369 1922
Size: 432 747 268 2650
Fragments arranged by size:
2650 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1236 1236
Size: 4097
EcoRI G'AATT_C
Cuts at: 1101 1101
Size: 4097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 4097
FspI TGC'GCA
Cuts at: 1878 1878
Size: 4097
HaeII r_GCGC'y
Cuts at: 1531 1779 2801 3780 1531
Size: 248 1022 979 1848
Fragments arranged by size:
1848 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 424 456 1939 1969 1971 2001 424
Size: 32 1483 30 2 30 2520
Fragments arranged by size:
2520 1483 32 30 30 2
HincII GTy'rAC
Cuts at: 1136 1136
Size: 4097
KasI G'GCGC_C
Cuts at: 1527 1775 1527
Size: 248 3849
KpnI G_GTAC'C
Cuts at: 1117 1117
Size: 4097
MluI A'CGCG_T
Cuts at: 3530 3530
Size: 4097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2768 2952 2768
Size: 184 3913
MscI TGG'CCA
Cuts at: 772 1858 772
Size: 1086 3011
MslI CAynn'nnrTG
Cuts at: 416 2086 2213 416
Size: 1670 127 2300
Fragments arranged by size:
2300 1670 127
MspA1I CmG'CkG
Cuts at: 1208 1522 1882 2895 3140 1208
Size: 314 360 1013 245 2165
Fragments arranged by size:
2165 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2278 2278
Size: 4097
NarI GG'CG_CC
Cuts at: 1528 1776 1528
Size: 248 3849
NcoI C'CATG_G
Cuts at: 845 845
Size: 4097
NgoMIV G'CCGG_C
Cuts at: 2276 2276
Size: 4097
NspI r_CATG'y
Cuts at: 680 1150 1650 2557 680
Size: 470 500 907 2220
Fragments arranged by size:
2220 907 500 470
PciI A'CATG_T
Cuts at: 2553 2553
Size: 4097
PflMI CCAn_nnn'nTGG
Cuts at: 256 256
Size: 4097
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 4065
PstI C_TGCA'G
Cuts at: 1144 1829 1144
Size: 685 3412
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 1522 1882 1522
Size: 360 3737
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 4097
SacI G_AGCT'C
Cuts at: 170 1111 170
Size: 941 3156
SalI G'TCGA_C
Cuts at: 1134 1134
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
SbfI CC_TGCA'GG
Cuts at: 1144 1144
Size: 4097
ScaI AGT'ACT
Cuts at: 3614 3614
Size: 4097
SfcI C'TryA_G
Cuts at: 33 1140 1825 2818 3009 33
Size: 1107 685 993 191 1121
Fragments arranged by size:
1121 1107 993 685 191
SfoI GGC'GCC
Cuts at: 1529 1777 1529
Size: 248 3849
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SmaI CCC'GGG
Cuts at: 153 1119 153
Size: 966 3131
SmlI C'TyrA_G
Cuts at: 377 1252 2167 2659 2921 377
Size: 875 915 492 262 1553
Fragments arranged by size:
1553 915 875 492 262
SpeI A'CTAG_T
Cuts at: 67 1038 67
Size: 971 3126
SphI G_CATG'C
Cuts at: 680 1150 1650 680
Size: 470 500 3127
Fragments arranged by size:
3127 500 470
StuI AGG'CCT
Cuts at: 711 711
Size: 4097
StyI C'CwwG_G
Cuts at: 845 1231 1585 845
Size: 386 354 3357
Fragments arranged by size:
3357 386 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 675 1680 1813 2272 2481 675
Size: 1005 133 459 209 2291
Fragments arranged by size:
2291 1005 459 209 133
TatI w'GTAC_w
Cuts at: 522 737 927 3612 522
Size: 215 190 2685 1007
Fragments arranged by size:
2685 1007 215 190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 4065
Tth111I GACn'n_nGTC
Cuts at: 1894 1894
Size: 4097
XbaI T'CTAG_A
Cuts at: 1128 1128
Size: 4097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1592 1592
Size: 4097
XmaI C'CCGG_G
Cuts at: 151 1117 151
Size: 966 3131
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI