Restriction map for
pF1KE0920
(Circular) (Six-base) MAPSORT of: pF1KE0920.seq Check: 8943 from: 1 to: 4052
pF1KE0920 4052 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 149 546 149
Size: 397 3655
AccI GT'mk_AC
Cuts at: 263 698 1090 263
Size: 435 392 3225
Fragments arranged by size:
3225 435 392
Acc65I G'GTAC_C
Cuts at: 1068 1068
Size: 4052
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 855 872 1877 2309 3056 3324 406
Size: 449 17 1005 432 747 268 1134
Fragments arranged by size:
1134 1005 747 449 432 268 17
AflIII A'CryG_T
Cuts at: 309 1404 2508 3485 309
Size: 1095 1104 977 876
Fragments arranged by size:
1104 1095 977 876
AgeI A'CCGG_T
Cuts at: 2440 2440
Size: 4052
AhdI GACnn_n'nnGTC
Cuts at: 669 669
Size: 4052
AleI CACnn'nnGTG
Cuts at: 361 361
Size: 4052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 181 379 181
Size: 198 3854
AlwNI CAG_nnn'CTG
Cuts at: 561 1357 2924 561
Size: 796 1567 1689
Fragments arranged by size:
1689 1567 796
ApaI G_GGCC'C
Cuts at: 232 1040 232
Size: 808 3244
ApaLI G'TGCA_C
Cuts at: 2822 2822
Size: 4052
ApoI r'AATT_y
Cuts at: 1056 3405 3661 1056
Size: 2349 256 1447
Fragments arranged by size:
2349 1447 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4052
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 359 399 475 719 1072 359
Size: 40 76 244 353 3339
Fragments arranged by size:
3339 353 244 76 40
BamHI G'GATC_C
Cuts at: 743 794 1077 743
Size: 51 283 3718
Fragments arranged by size:
3718 283 51
BanI G'GyrC_C
Cuts at: 183 1068 1482 1730 1765 183
Size: 885 414 248 35 2470
Fragments arranged by size:
2470 885 414 248 35
BanII G_rGCy'C
Cuts at: 206 232 400 522 653 1040 1066 2096 206
Size: 26 168 122 131 387 26 1030 2162
Fragments arranged by size:
2162 1030 387 168 131 122 26 26
BbeI G_GCGC'C
Cuts at: 187 1486 1734 187
Size: 1299 248 2505
Fragments arranged by size:
2505 1299 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4018
BciVI GTATCCnnnnn_n'
Cuts at: 1950 2711 4052 1950
Size: 761 1341 1950
Fragments arranged by size:
1950 1341 761
BclI T'GATC_A
Cuts at: 1572 1572
Size: 4052
BglII A'GATC_T
Cuts at: 489 1567 3604 489
Size: 1078 2037 937
Fragments arranged by size:
2037 1078 937
BlpI GC'TnA_GC
Cuts at: 1164 1164
Size: 4052
Bme1580I G_kGCm'C
Cuts at: 232 368 1040 1677 1770 2826 232
Size: 136 672 637 93 1056 1458
Fragments arranged by size:
1458 1056 672 637 136 93
BmrI ACTGGGnnnn_n'
Cuts at: 247 345 1264 1345 1680 3824 247
Size: 98 919 81 335 2144 475
Fragments arranged by size:
2144 919 475 335 98 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 487 583 719 229
Size: 258 96 136 3562
Fragments arranged by size:
3562 258 136 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 670 1228 2107 2599 2897 637
Size: 33 558 879 492 298 1792
Fragments arranged by size:
1792 879 558 492 298 33
BsaI GGTCTCn'nnnn_
Cuts at: 888 921 3756 888
Size: 33 2835 1184
Fragments arranged by size:
2835 1184 33
BsaAI yAC'GTr
Cuts at: 312 1405 312
Size: 1093 2959
BsaBI GATnn'nnATC
Cuts at: 6 1566 3603 6
Size: 1560 2037 455
Fragments arranged by size:
2037 1560 455
BsaHI Gr'CG_yC
Cuts at: 184 413 1483 1731 2433 3929 184
Size: 229 1070 248 702 1496 307
Fragments arranged by size:
1496 1070 702 307 248 229
BsaWI w'CCGG_w
Cuts at: 323 1241 1762 2440 2714 2861 3272 323
Size: 918 521 678 274 147 411 1103
Fragments arranged by size:
1103 918 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 202 232 202
Size: 30 4022
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 544 544
Size: 4052
BseYI C'CCAG_C
Cuts at: 1039 1477 2235 2812 3308 1039
Size: 438 758 577 496 1783
Fragments arranged by size:
1783 758 577 496 438
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 151
Size: 4052
BsiEI CG_ry'CG
Cuts at: 104 1594 1640 2848 104
Size: 1490 46 1208 1308
Fragments arranged by size:
1490 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 400 599 653 1066 1844 2034 2826 400
Size: 199 54 413 778 190 792 1626
Fragments arranged by size:
1626 792 778 413 199 190 54
BsmBI CGTCTCn'nnnn_
Cuts at: 285 285
Size: 4052
Bsp1286I G_dGCh'C
Cuts at: 206 232 368 400 522 599 653 1040 1066
Size: 26 136 32 122 77 54 387 26
Cuts at: 1066 1677 1770 1844 2034 2096 2826 206
Size: 611 93 74 190 62 730 1432
Fragments arranged by size:
1432 730 611 387 190 136 122 93
77 74 62 54 32 26 26
BspEI T'CCGG_A
Cuts at: 3272 3272
Size: 4052
BspHI T'CATG_A
Cuts at: 3228 3228
Size: 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 149 173 546 758 1102 1316 1618 1999 149
Size: 24 373 212 344 214 302 381 2202
Fragments arranged by size:
2202 381 373 344 302 214 212 24
BsrBI CCG'CTC
Cuts at: 50 2344 2398 3882 4047 50
Size: 2294 54 1484 165 55
Fragments arranged by size:
2294 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1964 1964
Size: 4052
BsrFI r'CCGG_y
Cuts at: 294 480 2050 2231 2440 294
Size: 186 1570 181 209 1906
Fragments arranged by size:
1906 1570 209 186 181
BssHII G'CGCG_C
Cuts at: 144 144
Size: 4052
BssSI C'ACGA_G
Cuts at: 2323 2681 2323
Size: 358 3694
BstAPI GCAn_nnn'nTGC
Cuts at: 1429 1429
Size: 4052
BstBI TT'CG_AA
Cuts at: 950 2413 950
Size: 1463 2589
BstXI CCAn_nnnn'nTGG
Cuts at: 180 375 180
Size: 195 3857
BstYI r'GATC_y
Cuts at: 1 489 743 794 1010 1077 1544 1567 1902
Size: 488 254 51 216 67 467 23 335
Cuts at: 1902 2148 3160 3246 3258 3604 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 488 467 449 346 335 254 246
216 86 67 51 23 12
BtgI C'CryG_G
Cuts at: 368 762 970 368
Size: 394 208 3450
Fragments arranged by size:
3450 394 208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 739 2002 2183 2346 739
Size: 1263 181 163 2445
Fragments arranged by size:
2445 1263 181 163
BtsI GCAGTG_nn'
Cuts at: 576 1295 576
Size: 719 3333
DraI TTT'AAA
Cuts at: 1051 1051
Size: 4052
DrdI GACnn_nn'nnGTC
Cuts at: 454 1758 2616 454
Size: 1304 858 1890
Fragments arranged by size:
1890 1304 858
EaeI y'GGCC_r
Cuts at: 299 1637 1811 2202 2229 3574 299
Size: 1338 174 391 27 1345 777
Fragments arranged by size:
1345 1338 777 391 174 27
EagI C'GGCC_G
Cuts at: 1637 1637
Size: 4052
EarI CTCTTCn'nnn_
Cuts at: 499 839 891 2075 2285 499
Size: 340 52 1184 210 2266
Fragments arranged by size:
2266 1184 340 210 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2260 2570 2716 3862 2260
Size: 310 146 1146 2450
Fragments arranged by size:
2450 1146 310 146
EcoICRI GAG'CTC
Cuts at: 398 651 1064 398
Size: 253 413 3386
Fragments arranged by size:
3386 413 253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 406 487 583 719 855 872 1877 2309
Size: 177 81 96 136 136 17 1005 432
Cuts at: 2309 3056 3324 229
Size: 747 268 957
Fragments arranged by size:
1005 957 747 432 268 177 136 136
96 81 17
EcoNI CCTnn'n_nnAGG
Cuts at: 526 994 526
Size: 468 3584
EcoO109I rG'GnC_Cy
Cuts at: 716 1036 1191 716
Size: 320 155 3577
Fragments arranged by size:
3577 320 155
EcoRI G'AATT_C
Cuts at: 1056 1056
Size: 4052
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 4052
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 187 682 1486 1734 2756 3735 187
Size: 495 804 248 1022 979 504
Fragments arranged by size:
1022 979 804 504 495 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 655 687 1894 1924 1926 1956 655
Size: 32 1207 30 2 30 2751
Fragments arranged by size:
2751 1207 32 30 30 2
HincII GTy'rAC
Cuts at: 1091 1091
Size: 4052
KasI G'GCGC_C
Cuts at: 183 1482 1730 183
Size: 1299 248 2505
Fragments arranged by size:
2505 1299 248
KpnI G_GTAC'C
Cuts at: 1072 1072
Size: 4052
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 308 356 2723 2907 308
Size: 48 2367 184 1453
Fragments arranged by size:
2367 1453 184 48
MscI TGG'CCA
Cuts at: 301 1813 301
Size: 1512 2540
MslI CAynn'nnrTG
Cuts at: 361 373 2041 2168 361
Size: 12 1668 127 2245
Fragments arranged by size:
2245 1668 127 12
MspA1I CmG'CkG
Cuts at: 245 1163 1477 1837 2850 3095 245
Size: 918 314 360 1013 245 1202
Fragments arranged by size:
1202 1013 918 360 314 245
NaeI GCC'GGC
Cuts at: 2233 2233
Size: 4052
NarI GG'CG_CC
Cuts at: 184 1483 1731 184
Size: 1299 248 2505
Fragments arranged by size:
2505 1299 248
NcoI C'CATG_G
Cuts at: 368 762 970 368
Size: 394 208 3450
Fragments arranged by size:
3450 394 208
NgoMIV G'CCGG_C
Cuts at: 2231 2231
Size: 4052
NspI r_CATG'y
Cuts at: 1105 1605 2512 1105
Size: 500 907 2645
Fragments arranged by size:
2645 907 500
PasI CC'CwG_GG
Cuts at: 789 789
Size: 4052
PciI A'CATG_T
Cuts at: 2508 2508
Size: 4052
PfoI T'CCnGG_A
Cuts at: 425 464 425
Size: 39 4013
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PmlI CAC'GTG
Cuts at: 312 312
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4020
PpuMI rG'GwC_Cy
Cuts at: 716 716
Size: 4052
PspOMI G'GGCC_C
Cuts at: 228 1036 228
Size: 808 3244
PspXI vC'TCGA_Gb
Cuts at: 359 359
Size: 4052
PstI C_TGCA'G
Cuts at: 1099 1784 1099
Size: 685 3367
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4052
PvuII CAG'CTG
Cuts at: 1477 1837 1477
Size: 360 3692
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 400 653 1066 400
Size: 253 413 3386
Fragments arranged by size:
3386 413 253
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SanDI GG'GwC_CC
Cuts at: 716 716
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
SbfI CC_TGCA'GG
Cuts at: 1099 1099
Size: 4052
ScaI AGT'ACT
Cuts at: 3569 3569
Size: 4052
SexAI A'CCwGG_T
Cuts at: 221 221
Size: 4052
SfcI C'TryA_G
Cuts at: 33 1095 1780 2773 2964 33
Size: 1062 685 993 191 1121
Fragments arranged by size:
1121 1062 993 685 191
SfoI GGC'GCC
Cuts at: 185 1484 1732 185
Size: 1299 248 2505
Fragments arranged by size:
2505 1299 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 1074 1074
Size: 4052
SmlI C'TyrA_G
Cuts at: 359 652 685 1207 2122 2614 2876 359
Size: 293 33 522 915 492 262 1535
Fragments arranged by size:
1535 915 522 492 293 262 33
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4052
SphI G_CATG'C
Cuts at: 1105 1605 1105
Size: 500 3552
StyI C'CwwG_G
Cuts at: 368 762 970 1186 1540 368
Size: 394 208 216 354 2880
Fragments arranged by size:
2880 394 354 216 208
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 249 1635 1768 2227 2436 249
Size: 1386 133 459 209 1865
Fragments arranged by size:
1865 1386 459 209 133
TatI w'GTAC_w
Cuts at: 3567 3567
Size: 4052
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4020
Tth111I GACn'n_nGTC
Cuts at: 694 1849 694
Size: 1155 2897
XbaI T'CTAG_A
Cuts at: 1083 1083
Size: 4052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 309 760 1547 309
Size: 451 787 2814
Fragments arranged by size:
2814 787 451
XhoI C'TCGA_G
Cuts at: 359 359
Size: 4052
XmaI C'CCGG_G
Cuts at: 1072 1072
Size: 4052
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI AvrII BaeI BbsI
BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsiWI BsmI
BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PshAI PsiI PsrI SacII
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI