Restriction map for
pF1KE0915
(Circular) (Six-base) MAPSORT of: pF1KE0915.seq Check: 7015 from: 1 to: 3902
pF1KE0915 3902 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 940 940
Size: 3902
Acc65I G'GTAC_C
Cuts at: 918 918
Size: 3902
AclI AA'CG_TT
Cuts at: 3742 3742
Size: 3902
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1727 2159 2906 3174 1727
Size: 432 747 268 2455
Fragments arranged by size:
2455 747 432 268
AflIII A'CryG_T
Cuts at: 1254 2358 3335 1254
Size: 1104 977 1821
Fragments arranged by size:
1821 1104 977
AgeI A'CCGG_T
Cuts at: 141 2290 141
Size: 2149 1753
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 453 453
Size: 3902
AlwNI CAG_nnn'CTG
Cuts at: 446 1207 2774 446
Size: 761 1567 1574
Fragments arranged by size:
1574 1567 761
ApaLI G'TGCA_C
Cuts at: 2672 2672
Size: 3902
ApoI r'AATT_y
Cuts at: 906 3255 3511 906
Size: 2349 256 1297
Fragments arranged by size:
2349 1297 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3902
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3902
AvaI C'yCGr_G
Cuts at: 922 922
Size: 3902
BamHI G'GATC_C
Cuts at: 836 927 836
Size: 91 3811
BanI G'GyrC_C
Cuts at: 412 577 918 1332 1580 1615 412
Size: 165 341 414 248 35 2699
Fragments arranged by size:
2699 414 341 248 165 35
BanII G_rGCy'C
Cuts at: 916 1946 916
Size: 1030 2872
BbeI G_GCGC'C
Cuts at: 1336 1584 1336
Size: 248 3654
BciVI GTATCCnnnnn_n'
Cuts at: 1800 2561 3902 1800
Size: 761 1341 1800
Fragments arranged by size:
1800 1341 761
BclI T'GATC_A
Cuts at: 1422 1422
Size: 3902
BfrBI ATG'CAT
Cuts at: 688 688
Size: 3902
BglII A'GATC_T
Cuts at: 183 1417 3454 183
Size: 1234 2037 631
Fragments arranged by size:
2037 1234 631
BlpI GC'TnA_GC
Cuts at: 1014 1014
Size: 3902
Bme1580I G_kGCm'C
Cuts at: 580 1527 1620 2676 580
Size: 947 93 1056 1806
Fragments arranged by size:
1806 1056 947 93
BmrI ACTGGGnnnn_n'
Cuts at: 1114 1195 1530 3674 1114
Size: 81 335 2144 1342
Fragments arranged by size:
2144 1342 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2269 2269
Size: 3902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 831 321
Size: 510 3392
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1078 1957 2449 2747 1078
Size: 879 492 298 2233
Fragments arranged by size:
2233 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 126 3606 126
Size: 3480 422
BsaAI yAC'GTr
Cuts at: 624 819 1255 624
Size: 195 436 3271
Fragments arranged by size:
3271 436 195
BsaBI GATnn'nnATC
Cuts at: 6 391 1416 3453 6
Size: 385 1025 2037 455
Fragments arranged by size:
2037 1025 455 385
BsaHI Gr'CG_yC
Cuts at: 1333 1581 2283 3779 1333
Size: 248 702 1496 1456
Fragments arranged by size:
1496 1456 702 248
BsaWI w'CCGG_w
Cuts at: 141 641 1091 1612 2290 2564 2711 3122 141
Size: 500 450 521 678 274 147 411 921
Fragments arranged by size:
921 678 521 500 450 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 198 228 308 338 349 379 788 818 198
Size: 30 80 30 11 30 409 30 3282
Fragments arranged by size:
3282 409 80 30 30 30 30 11
BseYI C'CCAG_C
Cuts at: 242 421 593 1327 2085 2662 3158 242
Size: 179 172 734 758 577 496 986
Fragments arranged by size:
986 758 734 577 496 179 172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 821 200
Size: 621 3281
BsiEI CG_ry'CG
Cuts at: 104 1444 1490 2698 104
Size: 1340 46 1208 1308
Fragments arranged by size:
1340 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 916 1694 1884 2676 916
Size: 778 190 792 2142
Fragments arranged by size:
2142 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 580 916 1527 1620 1694 1884 1946 2676 580
Size: 336 611 93 74 190 62 730 1806
Fragments arranged by size:
1806 730 611 336 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3122 3122
Size: 3902
BspHI T'CATG_A
Cuts at: 3078 3078
Size: 3902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 413 952 1166 1468 1849 413
Size: 539 214 302 381 2466
Fragments arranged by size:
2466 539 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2194 2248 3732 3897 50
Size: 2144 54 1484 165 55
Fragments arranged by size:
2144 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 359 475 1814 359
Size: 116 1339 2447
Fragments arranged by size:
2447 1339 116
BsrFI r'CCGG_y
Cuts at: 141 1900 2081 2290 141
Size: 1759 181 209 1753
Fragments arranged by size:
1759 1753 209 181
BssSI C'ACGA_G
Cuts at: 2173 2531 2173
Size: 358 3544
BstAPI GCAn_nnn'nTGC
Cuts at: 1279 1279
Size: 3902
BstBI TT'CG_AA
Cuts at: 489 2263 489
Size: 1774 2128
BstXI CCAn_nnnn'nTGG
Cuts at: 126 126
Size: 3902
BstYI r'GATC_y
Cuts at: 1 183 192 836 927 1394 1417 1752 1998
Size: 182 9 644 91 467 23 335 246
Cuts at: 1998 3010 3096 3108 3454 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 644 467 449 346 335 246 182
91 86 23 12 9
BtgI C'CryG_G
Cuts at: 107 312 416 572 107
Size: 205 104 156 3437
Fragments arranged by size:
3437 205 156 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1852 2033 2196 1852
Size: 181 163 3558
Fragments arranged by size:
3558 181 163
BtsI GCAGTG_nn'
Cuts at: 1145 1145
Size: 3902
DraI TTT'AAA
Cuts at: 901 901
Size: 3902
DrdI GACnn_nn'nnGTC
Cuts at: 173 1608 2466 173
Size: 1435 858 1609
Fragments arranged by size:
1609 1435 858
EaeI y'GGCC_r
Cuts at: 230 1487 1661 2052 2079 3424 230
Size: 1257 174 391 27 1345 708
Fragments arranged by size:
1345 1257 708 391 174 27
EagI C'GGCC_G
Cuts at: 1487 1487
Size: 3902
EarI CTCTTCn'nnn_
Cuts at: 251 1925 2135 251
Size: 1674 210 2018
Fragments arranged by size:
2018 1674 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 298 2110 2420 2566 3712 298
Size: 1812 310 146 1146 488
Fragments arranged by size:
1812 1146 488 310 146
EcoICRI GAG'CTC
Cuts at: 914 914
Size: 3902
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 831 1727 2159 2906 3174 321
Size: 510 896 432 747 268 1049
Fragments arranged by size:
1049 896 747 510 432 268
EcoO109I rG'GnC_Cy
Cuts at: 607 1041 607
Size: 434 3468
EcoRI G'AATT_C
Cuts at: 906 906
Size: 3902
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1921 1921
Size: 3902
FspI TGC'GCA
Cuts at: 1683 1683
Size: 3902
HaeII r_GCGC'y
Cuts at: 115 1336 1584 2606 3585 115
Size: 1221 248 1022 979 432
Fragments arranged by size:
1221 1022 979 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 775 788 807 820 1744 1774 1776 1806 775
Size: 13 19 13 924 30 2 30 2871
Fragments arranged by size:
2871 924 30 30 19 13 13 2
HincII GTy'rAC
Cuts at: 807 941 807
Size: 134 3768
KasI G'GCGC_C
Cuts at: 1332 1580 1332
Size: 248 3654
KpnI G_GTAC'C
Cuts at: 922 922
Size: 3902
MluI A'CGCG_T
Cuts at: 3335 3335
Size: 3902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 107 2573 2757 107
Size: 2466 184 1252
Fragments arranged by size:
2466 1252 184
MscI TGG'CCA
Cuts at: 1663 1663
Size: 3902
MslI CAynn'nnrTG
Cuts at: 1891 2018 1891
Size: 127 3775
MspA1I CmG'CkG
Cuts at: 331 349 433 517 1013 1327 1687 2700 2945
Size: 18 84 84 496 314 360 1013 245
Cuts at: 2945 331
Size: 1288
Fragments arranged by size:
1288 1013 496 360 314 245 84 84
18
NaeI GCC'GGC
Cuts at: 2083 2083
Size: 3902
NarI GG'CG_CC
Cuts at: 1333 1581 1333
Size: 248 3654
NcoI C'CATG_G
Cuts at: 107 312 416 572 107
Size: 205 104 156 3437
Fragments arranged by size:
3437 205 156 104
NdeI CA'TA_TG
Cuts at: 690 690
Size: 3902
NgoMIV G'CCGG_C
Cuts at: 2081 2081
Size: 3902
NsiI A_TGCA'T
Cuts at: 690 690
Size: 3902
NspI r_CATG'y
Cuts at: 955 1455 2362 955
Size: 500 907 2495
Fragments arranged by size:
2495 907 500
PciI A'CATG_T
Cuts at: 2358 2358
Size: 3902
PmeI GTTT'AAAC
Cuts at: 901 901
Size: 3902
PmlI CAC'GTG
Cuts at: 819 819
Size: 3902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3041 3073 3041
Size: 32 3870
PstI C_TGCA'G
Cuts at: 949 1634 949
Size: 685 3217
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3902
PvuII CAG'CTG
Cuts at: 331 517 1327 1687 331
Size: 186 810 360 2546
Fragments arranged by size:
2546 810 360 186
RsrII CG'GwC_CG
Cuts at: 2097 2097
Size: 3902
SacI G_AGCT'C
Cuts at: 916 916
Size: 3902
SalI G'TCGA_C
Cuts at: 939 939
Size: 3902
SapI GCTCTTCn'nnn_
Cuts at: 251 1925 2135 251
Size: 1674 210 2018
Fragments arranged by size:
2018 1674 210
SbfI CC_TGCA'GG
Cuts at: 949 949
Size: 3902
ScaI AGT'ACT
Cuts at: 3419 3419
Size: 3902
SfcI C'TryA_G
Cuts at: 33 344 509 665 777 945 1630 2623 2814
Size: 311 165 156 112 168 685 993 191
Cuts at: 2814 33
Size: 1121
Fragments arranged by size:
1121 993 685 311 191 168 165 156
112
SfoI GGC'GCC
Cuts at: 1334 1582 1334
Size: 248 3654
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3902
SmaI CCC'GGG
Cuts at: 924 924
Size: 3902
SmlI C'TyrA_G
Cuts at: 1057 1972 2464 2726 1057
Size: 915 492 262 2233
Fragments arranged by size:
2233 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3902
SphI G_CATG'C
Cuts at: 955 1455 955
Size: 500 3402
StuI AGG'CCT
Cuts at: 739 739
Size: 3902
StyI C'CwwG_G
Cuts at: 107 312 416 572 1036 1390 107
Size: 205 104 156 464 354 2619
Fragments arranged by size:
2619 464 354 205 156 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1485 1618 2077 2286 1485
Size: 133 459 209 3101
Fragments arranged by size:
3101 459 209 133
TatI w'GTAC_w
Cuts at: 551 3417 551
Size: 2866 1036
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 198 230 3419 3451 198
Size: 32 3189 32 649
Fragments arranged by size:
3189 649 32 32
Tth111I GACn'n_nGTC
Cuts at: 1699 1699
Size: 3902
XbaI T'CTAG_A
Cuts at: 933 933
Size: 3902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 855 1397 855
Size: 542 3360
XmaI C'CCGG_G
Cuts at: 922 922
Size: 3902
XmnI GAAnn'nnTTC
Cuts at: 275 275
Size: 3902
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PmeI PmlI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
PacI PasI PflMI PfoI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI