Restriction map for
pF1KE0913
(Circular) (Six-base) MAPSORT of: pF1KE0913.seq Check: 5947 from: 1 to: 3875
pF1KE0913 3875 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 913 913
Size: 3875
Acc65I G'GTAC_C
Cuts at: 891 891
Size: 3875
AclI AA'CG_TT
Cuts at: 3715 3715
Size: 3875
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1700 2132 2879 3147 1700
Size: 432 747 268 2428
Fragments arranged by size:
2428 747 432 268
AfeI AGC'GCT
Cuts at: 165 409 165
Size: 244 3631
AflIII A'CryG_T
Cuts at: 195 1227 2331 3308 195
Size: 1032 1104 977 762
Fragments arranged by size:
1104 1032 977 762
AgeI A'CCGG_T
Cuts at: 2263 2263
Size: 3875
AhdI GACnn_n'nnGTC
Cuts at: 785 785
Size: 3875
AleI CACnn'nnGTG
Cuts at: 574 574
Size: 3875
AlwNI CAG_nnn'CTG
Cuts at: 1180 2747 1180
Size: 1567 2308
ApaLI G'TGCA_C
Cuts at: 2645 2645
Size: 3875
ApoI r'AATT_y
Cuts at: 322 879 3228 3484 322
Size: 557 2349 256 713
Fragments arranged by size:
2349 713 557 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3875
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
AvaI C'yCGr_G
Cuts at: 895 895
Size: 3875
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 419 452 419
Size: 33 3842
BamHI G'GATC_C
Cuts at: 900 900
Size: 3875
BanI G'GyrC_C
Cuts at: 891 1305 1553 1588 891
Size: 414 248 35 3178
Fragments arranged by size:
3178 414 248 35
BanII G_rGCy'C
Cuts at: 488 889 1919 488
Size: 401 1030 2444
Fragments arranged by size:
2444 1030 401
BbeI G_GCGC'C
Cuts at: 1309 1557 1309
Size: 248 3627
BbsI GAAGACnn'nnnn_
Cuts at: 582 611 582
Size: 29 3846
BciVI GTATCCnnnnn_n'
Cuts at: 1773 2534 3875 1773
Size: 761 1341 1773
Fragments arranged by size:
1773 1341 761
BclI T'GATC_A
Cuts at: 200 1395 200
Size: 1195 2680
BglII A'GATC_T
Cuts at: 1390 3427 1390
Size: 2037 1838
BlpI GC'TnA_GC
Cuts at: 987 987
Size: 3875
Bme1580I G_kGCm'C
Cuts at: 771 1500 1593 2649 771
Size: 729 93 1056 1997
Fragments arranged by size:
1997 1056 729 93
BmrI ACTGGGnnnn_n'
Cuts at: 693 723 1087 1168 1503 3647 693
Size: 30 364 81 335 2144 921
Fragments arranged by size:
2144 921 364 335 81 30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 3875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 425
Size: 3875
Bpu10I CC'TnA_GC
Cuts at: 167 167
Size: 3875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 691 1051 1930 2422 2720 335
Size: 356 360 879 492 298 1490
Fragments arranged by size:
1490 879 492 360 356 298
BsaI GGTCTCn'nnnn_
Cuts at: 3579 3579
Size: 3875
BsaAI yAC'GTr
Cuts at: 542 1228 542
Size: 686 3189
BsaBI GATnn'nnATC
Cuts at: 6 1389 3426 6
Size: 1383 2037 455
Fragments arranged by size:
2037 1383 455
BsaHI Gr'CG_yC
Cuts at: 1306 1554 2256 3752 1306
Size: 248 702 1496 1429
Fragments arranged by size:
1496 1429 702 248
BsaWI w'CCGG_w
Cuts at: 1064 1585 2263 2537 2684 3095 1064
Size: 521 678 274 147 411 1844
Fragments arranged by size:
1844 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 113 143 113
Size: 30 3845
BseYI C'CCAG_C
Cuts at: 1300 2058 2635 3131 1300
Size: 758 577 496 2044
Fragments arranged by size:
2044 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1417 1463 2671 104
Size: 1313 46 1208 1308
Fragments arranged by size:
1313 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 458 683 889 1667 1857 2649 458
Size: 225 206 778 190 792 1684
Fragments arranged by size:
1684 792 778 225 206 190
BsmBI CGTCTCn'nnnn_
Cuts at: 295 295
Size: 3875
Bsp1286I G_dGCh'C
Cuts at: 458 488 683 771 889 1500 1593 1667 1857
Size: 30 195 88 118 611 93 74 190
Cuts at: 1857 1919 2649 458
Size: 62 730 1684
Fragments arranged by size:
1684 730 611 195 190 118 93 88
74 62 30
BspEI T'CCGG_A
Cuts at: 3095 3095
Size: 3875
BspHI T'CATG_A
Cuts at: 398 3051 398
Size: 2653 1222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 925 1139 1441 1822 925
Size: 214 302 381 2978
Fragments arranged by size:
2978 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2167 2221 3705 3870 50
Size: 2117 54 1484 165 55
Fragments arranged by size:
2117 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 849 1787 849
Size: 938 2937
BsrFI r'CCGG_y
Cuts at: 1873 2054 2263 1873
Size: 181 209 3485
Fragments arranged by size:
3485 209 181
BssSI C'ACGA_G
Cuts at: 2146 2504 2146
Size: 358 3517
BstAPI GCAn_nnn'nTGC
Cuts at: 1252 1252
Size: 3875
BstBI TT'CG_AA
Cuts at: 549 2236 549
Size: 1687 2188
BstYI r'GATC_y
Cuts at: 1 900 1367 1390 1725 1971 2983 3069 3081
Size: 899 467 23 335 246 1012 86 12
Cuts at: 3081 3427 1
Size: 346 449
Fragments arranged by size:
1012 899 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3875
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1825 2006 2169 1825
Size: 181 163 3531
Fragments arranged by size:
3531 181 163
BtsI GCAGTG_nn'
Cuts at: 1118 1118
Size: 3875
DraI TTT'AAA
Cuts at: 874 874
Size: 3875
DrdI GACnn_nn'nnGTC
Cuts at: 1581 2439 1581
Size: 858 3017
EaeI y'GGCC_r
Cuts at: 110 362 1460 1634 2025 2052 3397 110
Size: 252 1098 174 391 27 1345 588
Fragments arranged by size:
1345 1098 588 391 252 174 27
EagI C'GGCC_G
Cuts at: 1460 1460
Size: 3875
EarI CTCTTCn'nnn_
Cuts at: 707 1898 2108 707
Size: 1191 210 2474
Fragments arranged by size:
2474 1191 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2083 2393 2539 3685 2083
Size: 310 146 1146 2273
Fragments arranged by size:
2273 1146 310 146
EcoICRI GAG'CTC
Cuts at: 887 887
Size: 3875
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 1700 2132 2879 3147 425
Size: 1275 432 747 268 1153
Fragments arranged by size:
1275 1153 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 131 131
Size: 3875
EcoO109I rG'GnC_Cy
Cuts at: 862 1014 862
Size: 152 3723
EcoRI G'AATT_C
Cuts at: 879 879
Size: 3875
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 628 1894 628
Size: 1266 2609
FspI TGC'GCA
Cuts at: 835 1656 835
Size: 821 3054
HaeII r_GCGC'y
Cuts at: 167 411 1309 1557 2579 3558 167
Size: 244 898 248 1022 979 484
Fragments arranged by size:
1022 979 898 484 248 244
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 128 150 160 182 771 803 1717 1747 1749
Size: 22 10 22 589 32 914 30 2
Cuts at: 1749 1779 128
Size: 30 2224
Fragments arranged by size:
2224 914 589 32 30 30 22 22
10 2
HincII GTy'rAC
Cuts at: 914 914
Size: 3875
KasI G'GCGC_C
Cuts at: 1305 1553 1305
Size: 248 3627
KpnI G_GTAC'C
Cuts at: 895 895
Size: 3875
MluI A'CGCG_T
Cuts at: 3308 3308
Size: 3875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 437 2546 2730 437
Size: 2109 184 1582
Fragments arranged by size:
2109 1582 184
MscI TGG'CCA
Cuts at: 112 364 1636 112
Size: 252 1272 2351
Fragments arranged by size:
2351 1272 252
MslI CAynn'nnrTG
Cuts at: 151 373 574 1864 1991 151
Size: 222 201 1290 127 2035
Fragments arranged by size:
2035 1290 222 201 127
MspA1I CmG'CkG
Cuts at: 986 1300 1660 2673 2918 986
Size: 314 360 1013 245 1943
Fragments arranged by size:
1943 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2056 2056
Size: 3875
NarI GG'CG_CC
Cuts at: 1306 1554 1306
Size: 248 3627
NcoI C'CATG_G
Cuts at: 107 107
Size: 3875
NgoMIV G'CCGG_C
Cuts at: 2054 2054
Size: 3875
NspI r_CATG'y
Cuts at: 199 928 1428 2335 199
Size: 729 500 907 1739
Fragments arranged by size:
1739 907 729 500
PasI CC'CwG_GG
Cuts at: 599 771 599
Size: 172 3703
PciI A'CATG_T
Cuts at: 195 2331 195
Size: 2136 1739
PmeI GTTT'AAAC
Cuts at: 874 874
Size: 3875
PmlI CAC'GTG
Cuts at: 542 542
Size: 3875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3014 3046 3014
Size: 32 3843
PstI C_TGCA'G
Cuts at: 673 922 1607 673
Size: 249 685 2941
Fragments arranged by size:
2941 685 249
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3875
PvuII CAG'CTG
Cuts at: 1300 1660 1300
Size: 360 3515
RsrII CG'GwC_CG
Cuts at: 2070 2070
Size: 3875
SacI G_AGCT'C
Cuts at: 889 889
Size: 3875
SalI G'TCGA_C
Cuts at: 912 912
Size: 3875
SapI GCTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
SbfI CC_TGCA'GG
Cuts at: 922 922
Size: 3875
ScaI AGT'ACT
Cuts at: 3392 3392
Size: 3875
SfcI C'TryA_G
Cuts at: 33 669 749 918 1603 2596 2787 33
Size: 636 80 169 685 993 191 1121
Fragments arranged by size:
1121 993 685 636 191 169 80
SfoI GGC'GCC
Cuts at: 1307 1555 1307
Size: 248 3627
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
SmaI CCC'GGG
Cuts at: 897 897
Size: 3875
SmlI C'TyrA_G
Cuts at: 350 706 1030 1945 2437 2699 350
Size: 356 324 915 492 262 1526
Fragments arranged by size:
1526 915 492 356 324 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3875
SphI G_CATG'C
Cuts at: 928 1428 928
Size: 500 3375
StyI C'CwwG_G
Cuts at: 107 1009 1363 107
Size: 902 354 2619
Fragments arranged by size:
2619 902 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1458 1591 2050 2259 1458
Size: 133 459 209 3074
Fragments arranged by size:
3074 459 209 133
TatI w'GTAC_w
Cuts at: 3390 3390
Size: 3875
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 113 145 3392 3424 113
Size: 32 3247 32 564
Fragments arranged by size:
3247 564 32 32
Tth111I GACn'n_nGTC
Cuts at: 1672 1672
Size: 3875
XbaI T'CTAG_A
Cuts at: 906 906
Size: 3875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 372 1370 372
Size: 998 2877
XmaI C'CCGG_G
Cuts at: 895 895
Size: 3875
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PmlI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AlfI AloI ApaI AscI AvrII
BbvCI BcgI BfrBI BglI BmgBI BmtI BseRI BsgI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI