Restriction map for
pF1KE0910
(Circular) (Six-base) MAPSORT of: pF1KE0910.seq Check: 5961 from: 1 to: 3806
pF1KE0910 3806 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 844 844
Size: 3806
Acc65I G'GTAC_C
Cuts at: 822 822
Size: 3806
AclI AA'CG_TT
Cuts at: 3646 3646
Size: 3806
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 1631 2063 2810 3078 466
Size: 1165 432 747 268 1194
Fragments arranged by size:
1194 1165 747 432 268
AfeI AGC'GCT
Cuts at: 536 536
Size: 3806
AflIII A'CryG_T
Cuts at: 1158 2262 3239 1158
Size: 1104 977 1725
Fragments arranged by size:
1725 1104 977
AgeI A'CCGG_T
Cuts at: 2194 2194
Size: 3806
AleI CACnn'nnGTG
Cuts at: 644 644
Size: 3806
AlwNI CAG_nnn'CTG
Cuts at: 226 741 1111 2678 226
Size: 515 370 1567 1354
Fragments arranged by size:
1567 1354 515 370
ApaLI G'TGCA_C
Cuts at: 2576 2576
Size: 3806
ApoI r'AATT_y
Cuts at: 810 3159 3415 810
Size: 2349 256 1201
Fragments arranged by size:
2349 1201 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3806
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3806
AvaI C'yCGr_G
Cuts at: 826 826
Size: 3806
BamHI G'GATC_C
Cuts at: 166 831 166
Size: 665 3141
BanI G'GyrC_C
Cuts at: 822 1236 1484 1519 822
Size: 414 248 35 3109
Fragments arranged by size:
3109 414 248 35
BanII G_rGCy'C
Cuts at: 767 820 1850 767
Size: 53 1030 2723
Fragments arranged by size:
2723 1030 53
BbeI G_GCGC'C
Cuts at: 1240 1488 1240
Size: 248 3558
BbsI GAAGACnn'nnnn_
Cuts at: 586 586
Size: 3806
BciVI GTATCCnnnnn_n'
Cuts at: 1704 2465 3806 1704
Size: 761 1341 1704
Fragments arranged by size:
1704 1341 761
BclI T'GATC_A
Cuts at: 1326 1326
Size: 3806
BglII A'GATC_T
Cuts at: 1321 3358 1321
Size: 2037 1769
BlpI GC'TnA_GC
Cuts at: 918 918
Size: 3806
Bme1580I G_kGCm'C
Cuts at: 1431 1524 2580 1431
Size: 93 1056 2657
Fragments arranged by size:
2657 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1018 1099 1434 3578 1018
Size: 81 335 2144 1246
Fragments arranged by size:
2144 1246 335 81
BmtI G_CTAG'C
Cuts at: 356 356
Size: 3806
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 3806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 607 505
Size: 102 3704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1861 2353 2651 982
Size: 879 492 298 2137
Fragments arranged by size:
2137 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 350 3510 350
Size: 3160 646
BsaAI yAC'GTr
Cuts at: 1159 1159
Size: 3806
BsaBI GATnn'nnATC
Cuts at: 6 1320 3357 6
Size: 1314 2037 455
Fragments arranged by size:
2037 1314 455
BsaHI Gr'CG_yC
Cuts at: 1237 1485 2187 3683 1237
Size: 248 702 1496 1360
Fragments arranged by size:
1496 1360 702 248
BsaWI w'CCGG_w
Cuts at: 511 995 1516 2194 2468 2615 3026 511
Size: 484 521 678 274 147 411 1291
Fragments arranged by size:
1291 678 521 484 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 521 551 521
Size: 30 3776
BseYI C'CCAG_C
Cuts at: 1231 1989 2566 3062 1231
Size: 758 577 496 1975
Fragments arranged by size:
1975 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 759 195
Size: 564 3242
BsiEI CG_ry'CG
Cuts at: 104 566 1348 1394 2602 104
Size: 462 782 46 1208 1308
Fragments arranged by size:
1308 1208 782 462 46
BsiHKAI G_wGCw'C
Cuts at: 820 1598 1788 2580 820
Size: 778 190 792 2046
Fragments arranged by size:
2046 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 767 820 1431 1524 1598 1788 1850 2580 767
Size: 53 611 93 74 190 62 730 1993
Fragments arranged by size:
1993 730 611 190 93 74 62 53
BspEI T'CCGG_A
Cuts at: 3026 3026
Size: 3806
BspHI T'CATG_A
Cuts at: 2982 2982
Size: 3806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 103 856 1070 1372 1753 103
Size: 753 214 302 381 2156
Fragments arranged by size:
2156 753 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2098 2152 3636 3801 50
Size: 2048 54 1484 165 55
Fragments arranged by size:
2048 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1718 1718
Size: 3806
BsrFI r'CCGG_y
Cuts at: 1804 1985 2194 1804
Size: 181 209 3416
Fragments arranged by size:
3416 209 181
BsrGI T'GTAC_A
Cuts at: 572 572
Size: 3806
BssSI C'ACGA_G
Cuts at: 2077 2435 2077
Size: 358 3448
BstAPI GCAn_nnn'nTGC
Cuts at: 1183 1183
Size: 3806
BstBI TT'CG_AA
Cuts at: 2167 2167
Size: 3806
BstYI r'GATC_y
Cuts at: 1 166 831 1298 1321 1656 1902 2914 3000
Size: 165 665 467 23 335 246 1012 86
Cuts at: 3000 3012 3358 1
Size: 12 346 449
Fragments arranged by size:
1012 665 467 449 346 335 246 165
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 296 296
Size: 3806
BtgI C'CryG_G
Cuts at: 107 551 107
Size: 444 3362
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1756 1937 2100 1756
Size: 181 163 3462
Fragments arranged by size:
3462 181 163
BtsI GCAGTG_nn'
Cuts at: 1049 1049
Size: 3806
DraI TTT'AAA
Cuts at: 654 805 654
Size: 151 3655
DrdI GACnn_nn'nnGTC
Cuts at: 1512 2370 1512
Size: 858 2948
EaeI y'GGCC_r
Cuts at: 563 616 1391 1565 1956 1983 3328 563
Size: 53 775 174 391 27 1345 1041
Fragments arranged by size:
1345 1041 775 391 174 53 27
EagI C'GGCC_G
Cuts at: 563 1391 563
Size: 828 2978
EarI CTCTTCn'nnn_
Cuts at: 755 1829 2039 755
Size: 1074 210 2522
Fragments arranged by size:
2522 1074 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2014 2324 2470 3616 2014
Size: 310 146 1146 2204
Fragments arranged by size:
2204 1146 310 146
EcoICRI GAG'CTC
Cuts at: 818 818
Size: 3806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 505 607 1631 2063 2810 3078 466
Size: 39 102 1024 432 747 268 1194
Fragments arranged by size:
1194 1024 747 432 268 102 39
EcoO109I rG'GnC_Cy
Cuts at: 945 945
Size: 3806
EcoRI G'AATT_C
Cuts at: 810 810
Size: 3806
EcoRV GAT'ATC
Cuts at: 273 273
Size: 3806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1825 1825
Size: 3806
FspI TGC'GCA
Cuts at: 1587 1587
Size: 3806
HaeII r_GCGC'y
Cuts at: 538 1240 1488 2510 3489 538
Size: 702 248 1022 979 855
Fragments arranged by size:
1022 979 855 702 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 641 673 1648 1678 1680 1710 641
Size: 32 975 30 2 30 2737
Fragments arranged by size:
2737 975 32 30 30 2
HincII GTy'rAC
Cuts at: 845 845
Size: 3806
KasI G'GCGC_C
Cuts at: 1236 1484 1236
Size: 248 3558
KpnI G_GTAC'C
Cuts at: 826 826
Size: 3806
MluI A'CGCG_T
Cuts at: 3239 3239
Size: 3806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2477 2661 2477
Size: 184 3622
MscI TGG'CCA
Cuts at: 618 1567 618
Size: 949 2857
MslI CAynn'nnrTG
Cuts at: 644 1795 1922 644
Size: 1151 127 2528
Fragments arranged by size:
2528 1151 127
MspA1I CmG'CkG
Cuts at: 417 917 1231 1591 2604 2849 417
Size: 500 314 360 1013 245 1374
Fragments arranged by size:
1374 1013 500 360 314 245
NaeI GCC'GGC
Cuts at: 1987 1987
Size: 3806
NarI GG'CG_CC
Cuts at: 1237 1485 1237
Size: 248 3558
NcoI C'CATG_G
Cuts at: 107 107
Size: 3806
NgoMIV G'CCGG_C
Cuts at: 1985 1985
Size: 3806
NheI G'CTAG_C
Cuts at: 352 352
Size: 3806
NspI r_CATG'y
Cuts at: 859 1359 2266 859
Size: 500 907 2399
Fragments arranged by size:
2399 907 500
PciI A'CATG_T
Cuts at: 2262 2262
Size: 3806
PmeI GTTT'AAAC
Cuts at: 805 805
Size: 3806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2945 2977 2945
Size: 32 3774
PshAI GACnn'nnGTC
Cuts at: 586 586
Size: 3806
PstI C_TGCA'G
Cuts at: 746 853 1538 746
Size: 107 685 3014
Fragments arranged by size:
3014 685 107
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3806
PvuII CAG'CTG
Cuts at: 1231 1591 1231
Size: 360 3446
RsrII CG'GwC_CG
Cuts at: 2001 2001
Size: 3806
SacI G_AGCT'C
Cuts at: 820 820
Size: 3806
SalI G'TCGA_C
Cuts at: 843 843
Size: 3806
SapI GCTCTTCn'nnn_
Cuts at: 755 1829 2039 755
Size: 1074 210 2522
Fragments arranged by size:
2522 1074 210
SbfI CC_TGCA'GG
Cuts at: 853 853
Size: 3806
ScaI AGT'ACT
Cuts at: 125 3323 125
Size: 3198 608
SfcI C'TryA_G
Cuts at: 33 185 742 849 1534 2527 2718 33
Size: 152 557 107 685 993 191 1121
Fragments arranged by size:
1121 993 685 557 191 152 107
SfoI GGC'GCC
Cuts at: 1238 1486 1238
Size: 248 3558
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3806
SmaI CCC'GGG
Cuts at: 828 828
Size: 3806
SmlI C'TyrA_G
Cuts at: 961 1876 2368 2630 961
Size: 915 492 262 2137
Fragments arranged by size:
2137 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3806
SphI G_CATG'C
Cuts at: 859 1359 859
Size: 500 3306
StyI C'CwwG_G
Cuts at: 107 940 1294 107
Size: 833 354 2619
Fragments arranged by size:
2619 833 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1389 1522 1981 2190 1389
Size: 133 459 209 3005
Fragments arranged by size:
3005 459 209 133
TatI w'GTAC_w
Cuts at: 123 419 572 3321 123
Size: 296 153 2749 608
Fragments arranged by size:
2749 608 296 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3323 3355 3323
Size: 32 3774
Tth111I GACn'n_nGTC
Cuts at: 1603 1603
Size: 3806
XbaI T'CTAG_A
Cuts at: 837 837
Size: 3806
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1301 1301
Size: 3806
XmaI C'CCGG_G
Cuts at: 826 826
Size: 3806
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PmeI PpiI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI Bpu10I
BseRI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI