Restriction map for
pF1KE0909
(Circular) (Six-base) MAPSORT of: pF1KE0909.seq Check: 283 from: 1 to: 3788
pF1KE0909 3788 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 372 372
Size: 3788
AccI GT'mk_AC
Cuts at: 599 826 599
Size: 227 3561
Acc65I G'GTAC_C
Cuts at: 804 804
Size: 3788
AclI AA'CG_TT
Cuts at: 3628 3628
Size: 3788
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 502 1613 2045 2792 3060 481
Size: 21 1111 432 747 268 1209
Fragments arranged by size:
1209 1111 747 432 268 21
AflIII A'CryG_T
Cuts at: 122 1140 2244 3221 122
Size: 1018 1104 977 689
Fragments arranged by size:
1104 1018 977 689
AgeI A'CCGG_T
Cuts at: 2176 2176
Size: 3788
AlwNI CAG_nnn'CTG
Cuts at: 147 1093 2660 147
Size: 946 1567 1275
Fragments arranged by size:
1567 1275 946
ApaI G_GGCC'C
Cuts at: 344 425 691 344
Size: 81 266 3441
Fragments arranged by size:
3441 266 81
ApaLI G'TGCA_C
Cuts at: 2558 2558
Size: 3788
ApoI r'AATT_y
Cuts at: 792 3141 3397 792
Size: 2349 256 1183
Fragments arranged by size:
2349 1183 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3788
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
AvaI C'yCGr_G
Cuts at: 176 244 621 808 176
Size: 68 377 187 3156
Fragments arranged by size:
3156 377 187 68
BamHI G'GATC_C
Cuts at: 813 813
Size: 3788
BanI G'GyrC_C
Cuts at: 255 804 1218 1466 1501 255
Size: 549 414 248 35 2542
Fragments arranged by size:
2542 549 414 248 35
BanII G_rGCy'C
Cuts at: 344 425 691 802 1832 344
Size: 81 266 111 1030 2300
Fragments arranged by size:
2300 1030 266 111 81
BbeI G_GCGC'C
Cuts at: 259 1222 1470 259
Size: 963 248 2577
Fragments arranged by size:
2577 963 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3754
BciVI GTATCCnnnnn_n'
Cuts at: 1686 2447 3788 1686
Size: 761 1341 1686
Fragments arranged by size:
1686 1341 761
BclI T'GATC_A
Cuts at: 1308 1308
Size: 3788
BglII A'GATC_T
Cuts at: 509 1303 3340 509
Size: 794 2037 957
Fragments arranged by size:
2037 957 794
BlpI GC'TnA_GC
Cuts at: 900 900
Size: 3788
Bme1580I G_kGCm'C
Cuts at: 245 344 425 691 1413 1506 2562 245
Size: 99 81 266 722 93 1056 1471
Fragments arranged by size:
1471 1056 722 266 99 93 81
BmrI ACTGGGnnnn_n'
Cuts at: 1000 1081 1416 3560 1000
Size: 81 335 2144 1228
Fragments arranged by size:
2144 1228 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 3788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 169
Size: 3788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 964 1843 2335 2633 532
Size: 432 879 492 298 1687
Fragments arranged by size:
1687 879 492 432 298
BsaI GGTCTCn'nnnn_
Cuts at: 760 3492 760
Size: 2732 1056
BsaAI yAC'GTr
Cuts at: 1141 1141
Size: 3788
BsaBI GATnn'nnATC
Cuts at: 6 1302 3339 6
Size: 1296 2037 455
Fragments arranged by size:
2037 1296 455
BsaHI Gr'CG_yC
Cuts at: 256 1219 1467 2169 3665 256
Size: 963 248 702 1496 379
Fragments arranged by size:
1496 963 702 379 248
BsaWI w'CCGG_w
Cuts at: 651 977 1498 2176 2450 2597 3008 651
Size: 326 521 678 274 147 411 1431
Fragments arranged by size:
1431 678 521 411 326 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 625 655 625
Size: 30 3758
BseYI C'CCAG_C
Cuts at: 111 132 336 1213 1971 2548 3044 111
Size: 21 204 877 758 577 496 855
Fragments arranged by size:
877 855 758 577 496 204 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 541 400
Size: 141 3647
BsiEI CG_ry'CG
Cuts at: 104 356 1330 1376 2584 104
Size: 252 974 46 1208 1308
Fragments arranged by size:
1308 1208 974 252 46
BsiHKAI G_wGCw'C
Cuts at: 802 1580 1770 2562 802
Size: 778 190 792 2028
Fragments arranged by size:
2028 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 245 344 425 691 802 1413 1506 1580 1770
Size: 99 81 266 111 611 93 74 190
Cuts at: 1770 1832 2562 245
Size: 62 730 1471
Fragments arranged by size:
1471 730 611 266 190 111 99 93
81 74 62
BspEI T'CCGG_A
Cuts at: 3008 3008
Size: 3788
BspHI T'CATG_A
Cuts at: 2964 2964
Size: 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 372 838 1052 1354 1735 372
Size: 466 214 302 381 2425
Fragments arranged by size:
2425 466 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2080 2134 3618 3783 50
Size: 2030 54 1484 165 55
Fragments arranged by size:
2030 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1700 1700
Size: 3788
BsrFI r'CCGG_y
Cuts at: 210 351 1786 1967 2176 210
Size: 141 1435 181 209 1822
Fragments arranged by size:
1822 1435 209 181 141
BssSI C'ACGA_G
Cuts at: 2059 2417 2059
Size: 358 3430
BstAPI GCAn_nnn'nTGC
Cuts at: 1165 1165
Size: 3788
BstBI TT'CG_AA
Cuts at: 2149 2149
Size: 3788
BstEII G'GTnAC_C
Cuts at: 384 384
Size: 3788
BstYI r'GATC_y
Cuts at: 1 281 435 509 813 1280 1303 1638 1884
Size: 280 154 74 304 467 23 335 246
Cuts at: 1884 2896 2982 2994 3340 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 304 280 246
154 86 74 23 12
BtgI C'CryG_G
Cuts at: 356 356
Size: 3788
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1738 1919 2082 1738
Size: 181 163 3444
Fragments arranged by size:
3444 181 163
BtsI GCAGTG_nn'
Cuts at: 221 1031 221
Size: 810 2978
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 183 218 183
Size: 35 3753
DraI TTT'AAA
Cuts at: 787 787
Size: 3788
DrdI GACnn_nn'nnGTC
Cuts at: 402 595 1494 2352 402
Size: 193 899 858 1838
Fragments arranged by size:
1838 899 858 193
EaeI y'GGCC_r
Cuts at: 314 353 1373 1547 1938 1965 3310 314
Size: 39 1020 174 391 27 1345 792
Fragments arranged by size:
1345 1020 792 391 174 39 27
EagI C'GGCC_G
Cuts at: 353 1373 353
Size: 1020 2768
EarI CTCTTCn'nnn_
Cuts at: 262 715 1811 2021 262
Size: 453 1096 210 2029
Fragments arranged by size:
2029 1096 453 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1996 2306 2452 3598 1996
Size: 310 146 1146 2186
Fragments arranged by size:
2186 1146 310 146
EcoICRI GAG'CTC
Cuts at: 800 800
Size: 3788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 481 502 1613 2045 2792 3060 169
Size: 312 21 1111 432 747 268 897
Fragments arranged by size:
1111 897 747 432 312 268 21
EcoO109I rG'GnC_Cy
Cuts at: 340 341 421 447 576 687 688 927 340
Size: 1 80 26 129 111 1 239 3201
Fragments arranged by size:
3201 239 129 111 80 26 1 1
EcoRI G'AATT_C
Cuts at: 792 792
Size: 3788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 291 531 1807 291
Size: 240 1276 2272
Fragments arranged by size:
2272 1276 240
FspI TGC'GCA
Cuts at: 1569 1569
Size: 3788
HaeII r_GCGC'y
Cuts at: 169 259 629 667 1222 1470 2492 3471 169
Size: 90 370 38 555 248 1022 979 486
Fragments arranged by size:
1022 979 555 486 370 248 90 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 494 526 1630 1660 1662 1692 494
Size: 32 1104 30 2 30 2590
Fragments arranged by size:
2590 1104 32 30 30 2
HincII GTy'rAC
Cuts at: 827 827
Size: 3788
KasI G'GCGC_C
Cuts at: 255 1218 1466 255
Size: 963 248 2577
Fragments arranged by size:
2577 963 248
KpnI G_GTAC'C
Cuts at: 808 808
Size: 3788
MluI A'CGCG_T
Cuts at: 122 3221 122
Size: 3099 689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 209 386 2459 2643 209
Size: 177 2073 184 1354
Fragments arranged by size:
2073 1354 184 177
MscI TGG'CCA
Cuts at: 316 1549 316
Size: 1233 2555
MslI CAynn'nnrTG
Cuts at: 1777 1904 1777
Size: 127 3661
MspA1I CmG'CkG
Cuts at: 144 291 542 899 1213 1573 2586 2831 144
Size: 147 251 357 314 360 1013 245 1101
Fragments arranged by size:
1101 1013 360 357 314 251 245 147
NaeI GCC'GGC
Cuts at: 212 1969 212
Size: 1757 2031
NarI GG'CG_CC
Cuts at: 256 1219 1467 256
Size: 963 248 2577
Fragments arranged by size:
2577 963 248
NgoMIV G'CCGG_C
Cuts at: 210 1967 210
Size: 1757 2031
NspI r_CATG'y
Cuts at: 841 1341 2248 841
Size: 500 907 2381
Fragments arranged by size:
2381 907 500
PciI A'CATG_T
Cuts at: 2244 2244
Size: 3788
PmeI GTTT'AAAC
Cuts at: 787 787
Size: 3788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2927 2959 2927
Size: 32 3756
PpuMI rG'GwC_Cy
Cuts at: 447 447
Size: 3788
PspOMI G'GGCC_C
Cuts at: 340 421 687 340
Size: 81 266 3441
Fragments arranged by size:
3441 266 81
PstI C_TGCA'G
Cuts at: 835 1520 835
Size: 685 3103
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3788
PvuII CAG'CTG
Cuts at: 144 291 1213 1573 144
Size: 147 922 360 2359
Fragments arranged by size:
2359 922 360 147
RsrII CG'GwC_CG
Cuts at: 1983 1983
Size: 3788
SacI G_AGCT'C
Cuts at: 802 802
Size: 3788
SalI G'TCGA_C
Cuts at: 825 825
Size: 3788
SapI GCTCTTCn'nnn_
Cuts at: 262 1811 2021 262
Size: 1549 210 2029
Fragments arranged by size:
2029 1549 210
SbfI CC_TGCA'GG
Cuts at: 835 835
Size: 3788
ScaI AGT'ACT
Cuts at: 3305 3305
Size: 3788
SexAI A'CCwGG_T
Cuts at: 467 467
Size: 3788
SfcI C'TryA_G
Cuts at: 33 831 1516 2509 2700 33
Size: 798 685 993 191 1121
Fragments arranged by size:
1121 993 798 685 191
SfoI GGC'GCC
Cuts at: 257 1220 1468 257
Size: 963 248 2577
Fragments arranged by size:
2577 963 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
SmaI CCC'GGG
Cuts at: 810 810
Size: 3788
SmlI C'TyrA_G
Cuts at: 547 943 1858 2350 2612 547
Size: 396 915 492 262 1723
Fragments arranged by size:
1723 915 492 396 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3788
SphI G_CATG'C
Cuts at: 841 1341 841
Size: 500 3288
StyI C'CwwG_G
Cuts at: 922 1276 922
Size: 354 3434
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1371 1504 1963 2172 1371
Size: 133 459 209 2987
Fragments arranged by size:
2987 459 209 133
TatI w'GTAC_w
Cuts at: 320 3303 320
Size: 2983 805
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 724 756 3305 3337 724
Size: 32 2549 32 1175
Fragments arranged by size:
2549 1175 32 32
Tth111I GACn'n_nGTC
Cuts at: 1585 1585
Size: 3788
XbaI T'CTAG_A
Cuts at: 819 819
Size: 3788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1283 114
Size: 1169 2619
XmaI C'CCGG_G
Cuts at: 808 808
Size: 3788
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI