Restriction map for
pF1KE0908
(Circular) (Six-base) MAPSORT of: pF1KE0908.seq Check: 9385 from: 1 to: 3788
pF1KE0908 3788 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 826 826
Size: 3788
Acc65I G'GTAC_C
Cuts at: 804 804
Size: 3788
AclI AA'CG_TT
Cuts at: 3628 3628
Size: 3788
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 1613 2045 2792 3060 691
Size: 922 432 747 268 1419
Fragments arranged by size:
1419 922 747 432 268
AflIII A'CryG_T
Cuts at: 1140 2244 3221 1140
Size: 1104 977 1707
Fragments arranged by size:
1707 1104 977
AgeI A'CCGG_T
Cuts at: 2176 2176
Size: 3788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 191 191
Size: 3788
AlwNI CAG_nnn'CTG
Cuts at: 214 534 591 748 1093 2660 214
Size: 320 57 157 345 1567 1342
Fragments arranged by size:
1567 1342 345 320 157 57
ApaI G_GGCC'C
Cuts at: 307 307
Size: 3788
ApaLI G'TGCA_C
Cuts at: 153 2558 153
Size: 2405 1383
ApoI r'AATT_y
Cuts at: 792 3141 3397 792
Size: 2349 256 1183
Fragments arranged by size:
2349 1183 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3788
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
AvaI C'yCGr_G
Cuts at: 808 808
Size: 3788
BamHI G'GATC_C
Cuts at: 813 813
Size: 3788
BanI G'GyrC_C
Cuts at: 167 804 1218 1466 1501 167
Size: 637 414 248 35 2454
Fragments arranged by size:
2454 637 414 248 35
BanII G_rGCy'C
Cuts at: 307 774 802 1832 307
Size: 467 28 1030 2263
Fragments arranged by size:
2263 1030 467 28
BbeI G_GCGC'C
Cuts at: 1222 1470 1222
Size: 248 3540
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 110 144 110
Size: 34 3754
BciVI GTATCCnnnnn_n'
Cuts at: 1686 2447 3788 1686
Size: 761 1341 1686
Fragments arranged by size:
1686 1341 761
BclI T'GATC_A
Cuts at: 1308 1308
Size: 3788
BglI GCCn_nnn'nGGC
Cuts at: 530 539 530
Size: 9 3779
BglII A'GATC_T
Cuts at: 1303 3340 1303
Size: 2037 1751
BlpI GC'TnA_GC
Cuts at: 488 900 488
Size: 412 3376
Bme1580I G_kGCm'C
Cuts at: 157 307 1413 1506 2562 157
Size: 150 1106 93 1056 1383
Fragments arranged by size:
1383 1106 1056 150 93
BmrI ACTGGGnnnn_n'
Cuts at: 1000 1081 1416 3560 1000
Size: 81 335 2144 1228
Fragments arranged by size:
2144 1228 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 3788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 511 628 725 277
Size: 234 117 97 3340
Fragments arranged by size:
3340 234 117 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 964 1843 2335 2633 706
Size: 258 879 492 298 1861
Fragments arranged by size:
1861 879 492 298 258
BsaI GGTCTCn'nnnn_
Cuts at: 3492 3492
Size: 3788
BsaAI yAC'GTr
Cuts at: 1141 1141
Size: 3788
BsaBI GATnn'nnATC
Cuts at: 6 1302 3339 6
Size: 1296 2037 455
Fragments arranged by size:
2037 1296 455
BsaHI Gr'CG_yC
Cuts at: 554 1219 1467 2169 3665 554
Size: 665 248 702 1496 677
Fragments arranged by size:
1496 702 677 665 248
BsaWI w'CCGG_w
Cuts at: 977 1498 2176 2450 2597 3008 977
Size: 521 678 274 147 411 1757
Fragments arranged by size:
1757 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 600 630 600
Size: 30 3758
BseYI C'CCAG_C
Cuts at: 280 537 584 1213 1971 2548 3044 280
Size: 257 47 629 758 577 496 1024
Fragments arranged by size:
1024 758 629 577 496 257 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 745 501
Size: 244 3544
BsiEI CG_ry'CG
Cuts at: 104 1330 1376 2584 104
Size: 1226 46 1208 1308
Fragments arranged by size:
1308 1226 1208 46
BsiHKAI G_wGCw'C
Cuts at: 157 164 263 802 1580 1770 2562 157
Size: 7 99 539 778 190 792 1383
Fragments arranged by size:
1383 792 778 539 190 99 7
Bsp1286I G_dGCh'C
Cuts at: 157 164 263 307 774 802 1413 1506 1580
Size: 7 99 44 467 28 611 93 74
Cuts at: 1580 1770 1832 2562 157
Size: 190 62 730 1383
Fragments arranged by size:
1383 730 611 467 190 99 93 74
62 44 28 7
BspEI T'CCGG_A
Cuts at: 3008 3008
Size: 3788
BspHI T'CATG_A
Cuts at: 2964 2964
Size: 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 838 1052 1354 1735 838
Size: 214 302 381 2891
Fragments arranged by size:
2891 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2080 2134 3618 3783 50
Size: 2030 54 1484 165 55
Fragments arranged by size:
2030 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 545 1700 545
Size: 1155 2633
BsrFI r'CCGG_y
Cuts at: 716 1786 1967 2176 716
Size: 1070 181 209 2328
Fragments arranged by size:
2328 1070 209 181
BssSI C'ACGA_G
Cuts at: 129 2059 2417 129
Size: 1930 358 1500
Fragments arranged by size:
1930 1500 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1165 1165
Size: 3788
BstBI TT'CG_AA
Cuts at: 2149 2149
Size: 3788
BstYI r'GATC_y
Cuts at: 1 813 1280 1303 1638 1884 2896 2982 2994
Size: 812 467 23 335 246 1012 86 12
Cuts at: 2994 3340 1
Size: 346 449
Fragments arranged by size:
1012 812 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3788
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1738 1919 2082 1738
Size: 181 163 3444
Fragments arranged by size:
3444 181 163
BtsI GCAGTG_nn'
Cuts at: 185 482 577 1031 185
Size: 297 95 454 2942
Fragments arranged by size:
2942 454 297 95
DraI TTT'AAA
Cuts at: 787 787
Size: 3788
DrdI GACnn_nn'nnGTC
Cuts at: 1494 2352 1494
Size: 858 2930
EaeI y'GGCC_r
Cuts at: 701 1373 1547 1938 1965 3310 701
Size: 672 174 391 27 1345 1179
Fragments arranged by size:
1345 1179 672 391 174 27
EagI C'GGCC_G
Cuts at: 1373 1373
Size: 3788
EarI CTCTTCn'nnn_
Cuts at: 1811 2021 1811
Size: 210 3578
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1996 2306 2452 3598 1996
Size: 310 146 1146 2186
Fragments arranged by size:
2186 1146 310 146
EcoICRI GAG'CTC
Cuts at: 800 800
Size: 3788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 511 628 691 725 1613 2045 2792 3060
Size: 234 117 63 34 888 432 747 268
Cuts at: 3060 277
Size: 1005
Fragments arranged by size:
1005 888 747 432 268 234 117 63
34
EcoO109I rG'GnC_Cy
Cuts at: 204 211 284 304 531 927 204
Size: 7 73 20 227 396 3065
Fragments arranged by size:
3065 396 227 73 20 7
EcoRI G'AATT_C
Cuts at: 792 792
Size: 3788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1807 1807
Size: 3788
FspI TGC'GCA
Cuts at: 1569 1569
Size: 3788
HaeII r_GCGC'y
Cuts at: 699 1222 1470 2492 3471 699
Size: 523 248 1022 979 1016
Fragments arranged by size:
1022 1016 979 523 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 723 755 1630 1660 1662 1692 723
Size: 32 875 30 2 30 2819
Fragments arranged by size:
2819 875 32 30 30 2
HincII GTy'rAC
Cuts at: 375 827 375
Size: 452 3336
KasI G'GCGC_C
Cuts at: 1218 1466 1218
Size: 248 3540
KpnI G_GTAC'C
Cuts at: 808 808
Size: 3788
MluI A'CGCG_T
Cuts at: 3221 3221
Size: 3788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2459 2643 2459
Size: 184 3604
MscI TGG'CCA
Cuts at: 1549 1549
Size: 3788
MslI CAynn'nnrTG
Cuts at: 1777 1904 1777
Size: 127 3661
MspA1I CmG'CkG
Cuts at: 591 748 899 1213 1573 2586 2831 591
Size: 157 151 314 360 1013 245 1548
Fragments arranged by size:
1548 1013 360 314 245 157 151
NaeI GCC'GGC
Cuts at: 1969 1969
Size: 3788
NarI GG'CG_CC
Cuts at: 1219 1467 1219
Size: 248 3540
NcoI C'CATG_G
Cuts at: 107 107
Size: 3788
NgoMIV G'CCGG_C
Cuts at: 1967 1967
Size: 3788
NspI r_CATG'y
Cuts at: 841 1341 2248 841
Size: 500 907 2381
Fragments arranged by size:
2381 907 500
PasI CC'CwG_GG
Cuts at: 208 362 208
Size: 154 3634
PciI A'CATG_T
Cuts at: 2244 2244
Size: 3788
PflMI CCAn_nnn'nTGG
Cuts at: 214 651 214
Size: 437 3351
PmeI GTTT'AAAC
Cuts at: 787 787
Size: 3788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2927 2959 2927
Size: 32 3756
PpuMI rG'GwC_Cy
Cuts at: 204 284 204
Size: 80 3708
PspOMI G'GGCC_C
Cuts at: 303 303
Size: 3788
PstI C_TGCA'G
Cuts at: 180 683 753 835 1520 180
Size: 503 70 82 685 2448
Fragments arranged by size:
2448 685 503 82 70
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3788
PvuII CAG'CTG
Cuts at: 748 1213 1573 748
Size: 465 360 2963
Fragments arranged by size:
2963 465 360
RsrII CG'GwC_CG
Cuts at: 1983 1983
Size: 3788
SacI G_AGCT'C
Cuts at: 802 802
Size: 3788
SalI G'TCGA_C
Cuts at: 825 825
Size: 3788
SanDI GG'GwC_CC
Cuts at: 204 204
Size: 3788
SapI GCTCTTCn'nnn_
Cuts at: 1811 2021 1811
Size: 210 3578
SbfI CC_TGCA'GG
Cuts at: 835 835
Size: 3788
ScaI AGT'ACT
Cuts at: 3305 3305
Size: 3788
SfcI C'TryA_G
Cuts at: 33 176 435 679 749 831 1516 2509 2700
Size: 143 259 244 70 82 685 993 191
Cuts at: 2700 33
Size: 1121
Fragments arranged by size:
1121 993 685 259 244 191 143 82
70
SfoI GGC'GCC
Cuts at: 1220 1468 1220
Size: 248 3540
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
SmaI CCC'GGG
Cuts at: 810 810
Size: 3788
SmlI C'TyrA_G
Cuts at: 721 943 1858 2350 2612 721
Size: 222 915 492 262 1897
Fragments arranged by size:
1897 915 492 262 222
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3788
SphI G_CATG'C
Cuts at: 841 1341 841
Size: 500 3288
StuI AGG'CCT
Cuts at: 433 433
Size: 3788
StyI C'CwwG_G
Cuts at: 107 704 922 1276 107
Size: 597 218 354 2619
Fragments arranged by size:
2619 597 354 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1371 1504 1963 2172 1371
Size: 133 459 209 2987
Fragments arranged by size:
2987 459 209 133
TatI w'GTAC_w
Cuts at: 3303 3303
Size: 3788
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 600 632 3305 3337 600
Size: 32 2673 32 1051
Fragments arranged by size:
2673 1051 32 32
Tth111I GACn'n_nGTC
Cuts at: 1585 1585
Size: 3788
XbaI T'CTAG_A
Cuts at: 819 819
Size: 3788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 375 592 1283 375
Size: 217 691 2880
Fragments arranged by size:
2880 691 217
XmaI C'CCGG_G
Cuts at: 808 808
Size: 3788
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI