Restriction map for
pF1KE0903
(Circular) (Six-base) MAPSORT of: pF1KE0903.seq Check: 4294 from: 1 to: 3617
pF1KE0903 3617 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 655 655
Size: 3617
Acc65I G'GTAC_C
Cuts at: 633 633
Size: 3617
AclI AA'CG_TT
Cuts at: 3457 3457
Size: 3617
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 283 500 1442 1874 2621 2889 197
Size: 86 217 942 432 747 268 925
Fragments arranged by size:
942 925 747 432 268 217 86
AflIII A'CryG_T
Cuts at: 969 2073 3050 969
Size: 1104 977 1536
Fragments arranged by size:
1536 1104 977
AgeI A'CCGG_T
Cuts at: 2005 2005
Size: 3617
AlwNI CAG_nnn'CTG
Cuts at: 922 2489 922
Size: 1567 2050
ApaLI G'TGCA_C
Cuts at: 2387 2387
Size: 3617
ApoI r'AATT_y
Cuts at: 317 538 621 2970 3226 317
Size: 221 83 2349 256 708
Fragments arranged by size:
2349 708 256 221 83
AseI AT'TA_AT
Cuts at: 20 20
Size: 3617
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3617
AvaI C'yCGr_G
Cuts at: 637 637
Size: 3617
BamHI G'GATC_C
Cuts at: 642 642
Size: 3617
BanI G'GyrC_C
Cuts at: 123 633 1047 1295 1330 123
Size: 510 414 248 35 2410
Fragments arranged by size:
2410 510 414 248 35
BanII G_rGCy'C
Cuts at: 631 1661 631
Size: 1030 2587
BbeI G_GCGC'C
Cuts at: 127 1051 1299 127
Size: 924 248 2445
Fragments arranged by size:
2445 924 248
BbsI GAAGACnn'nnnn_
Cuts at: 161 161
Size: 3617
BciVI GTATCCnnnnn_n'
Cuts at: 1515 2276 3617 1515
Size: 761 1341 1515
Fragments arranged by size:
1515 1341 761
BclI T'GATC_A
Cuts at: 1137 1137
Size: 3617
BglII A'GATC_T
Cuts at: 294 1132 3169 294
Size: 838 2037 742
Fragments arranged by size:
2037 838 742
BlpI GC'TnA_GC
Cuts at: 729 729
Size: 3617
Bme1580I G_kGCm'C
Cuts at: 1242 1335 2391 1242
Size: 93 1056 2468
Fragments arranged by size:
2468 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 829 910 1245 3389 829
Size: 81 335 2144 1057
Fragments arranged by size:
2144 1057 335 81
BmtI G_CTAG'C
Cuts at: 494 494
Size: 3617
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1984 1984
Size: 3617
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 1672 2164 2462 793
Size: 879 492 298 1948
Fragments arranged by size:
1948 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3321 3321
Size: 3617
BsaAI yAC'GTr
Cuts at: 970 970
Size: 3617
BsaBI GATnn'nnATC
Cuts at: 6 1131 3168 6
Size: 1125 2037 455
Fragments arranged by size:
2037 1125 455
BsaHI Gr'CG_yC
Cuts at: 124 1048 1296 1998 3494 124
Size: 924 248 702 1496 247
Fragments arranged by size:
1496 924 702 248 247
BsaWI w'CCGG_w
Cuts at: 253 806 1327 2005 2279 2426 2837 253
Size: 553 521 678 274 147 411 1033
Fragments arranged by size:
1033 678 553 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1042 1800 2377 2873 1042
Size: 758 577 496 1786
Fragments arranged by size:
1786 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1159 1205 2413 104
Size: 1055 46 1208 1308
Fragments arranged by size:
1308 1208 1055 46
BsiHKAI G_wGCw'C
Cuts at: 631 1409 1599 2391 631
Size: 778 190 792 1857
Fragments arranged by size:
1857 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 631 1242 1335 1409 1599 1661 2391 631
Size: 611 93 74 190 62 730 1857
Fragments arranged by size:
1857 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2837 2837
Size: 3617
BspHI T'CATG_A
Cuts at: 2793 2793
Size: 3617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 667 881 1183 1564 667
Size: 214 302 381 2720
Fragments arranged by size:
2720 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1909 1963 3447 3612 50
Size: 1859 54 1484 165 55
Fragments arranged by size:
1859 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 340 1529 340
Size: 1189 2428
BsrFI r'CCGG_y
Cuts at: 120 1615 1796 2005 120
Size: 1495 181 209 1732
Fragments arranged by size:
1732 1495 209 181
BssSI C'ACGA_G
Cuts at: 1888 2246 1888
Size: 358 3259
BstAPI GCAn_nnn'nTGC
Cuts at: 994 994
Size: 3617
BstBI TT'CG_AA
Cuts at: 1978 1978
Size: 3617
BstYI r'GATC_y
Cuts at: 1 294 510 570 642 1109 1132 1467 1713
Size: 293 216 60 72 467 23 335 246
Cuts at: 1713 2725 2811 2823 3169 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 293 246 216
86 72 60 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3617
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1567 1748 1911 1567
Size: 181 163 3273
Fragments arranged by size:
3273 181 163
BtsI GCAGTG_nn'
Cuts at: 860 860
Size: 3617
DraI TTT'AAA
Cuts at: 234 616 234
Size: 382 3235
DrdI GACnn_nn'nnGTC
Cuts at: 1323 2181 1323
Size: 858 2759
EaeI y'GGCC_r
Cuts at: 1202 1376 1767 1794 3139 1202
Size: 174 391 27 1345 1680
Fragments arranged by size:
1680 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1202 1202
Size: 3617
EarI CTCTTCn'nnn_
Cuts at: 1640 1850 1640
Size: 210 3407
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1825 2135 2281 3427 1825
Size: 310 146 1146 2015
Fragments arranged by size:
2015 1146 310 146
EcoICRI GAG'CTC
Cuts at: 629 629
Size: 3617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 283 500 1442 1874 2621 2889 197
Size: 86 217 942 432 747 268 925
Fragments arranged by size:
942 925 747 432 268 217 86
EcoO109I rG'GnC_Cy
Cuts at: 756 756
Size: 3617
EcoRI G'AATT_C
Cuts at: 538 621 538
Size: 83 3534
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1636 1636
Size: 3617
FspI TGC'GCA
Cuts at: 1398 1398
Size: 3617
HaeII r_GCGC'y
Cuts at: 127 419 1051 1299 2321 3300 127
Size: 292 632 248 1022 979 444
Fragments arranged by size:
1022 979 632 444 292 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 495 527 562 594 1459 1489 1491 1521 495
Size: 32 35 32 865 30 2 30 2591
Fragments arranged by size:
2591 865 35 32 32 30 30 2
HincII GTy'rAC
Cuts at: 656 656
Size: 3617
HindIII A'AGCT_T
Cuts at: 202 202
Size: 3617
KasI G'GCGC_C
Cuts at: 123 1047 1295 123
Size: 924 248 2445
Fragments arranged by size:
2445 924 248
KpnI G_GTAC'C
Cuts at: 637 637
Size: 3617
MluI A'CGCG_T
Cuts at: 3050 3050
Size: 3617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2288 2472 2288
Size: 184 3433
MscI TGG'CCA
Cuts at: 1378 1378
Size: 3617
MslI CAynn'nnrTG
Cuts at: 1606 1733 1606
Size: 127 3490
MspA1I CmG'CkG
Cuts at: 728 1042 1402 2415 2660 728
Size: 314 360 1013 245 1685
Fragments arranged by size:
1685 1013 360 314 245
NaeI GCC'GGC
Cuts at: 122 1798 122
Size: 1676 1941
NarI GG'CG_CC
Cuts at: 124 1048 1296 124
Size: 924 248 2445
Fragments arranged by size:
2445 924 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3617
NdeI CA'TA_TG
Cuts at: 348 348
Size: 3617
NgoMIV G'CCGG_C
Cuts at: 120 1796 120
Size: 1676 1941
NheI G'CTAG_C
Cuts at: 490 490
Size: 3617
NspI r_CATG'y
Cuts at: 670 1170 2077 670
Size: 500 907 2210
Fragments arranged by size:
2210 907 500
PciI A'CATG_T
Cuts at: 2073 2073
Size: 3617
PmeI GTTT'AAAC
Cuts at: 616 616
Size: 3617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2756 2788 2756
Size: 32 3585
PstI C_TGCA'G
Cuts at: 664 1349 664
Size: 685 2932
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3617
PvuII CAG'CTG
Cuts at: 1042 1402 1042
Size: 360 3257
RsrII CG'GwC_CG
Cuts at: 1812 1812
Size: 3617
SacI G_AGCT'C
Cuts at: 631 631
Size: 3617
SalI G'TCGA_C
Cuts at: 654 654
Size: 3617
SapI GCTCTTCn'nnn_
Cuts at: 1640 1850 1640
Size: 210 3407
SbfI CC_TGCA'GG
Cuts at: 664 664
Size: 3617
ScaI AGT'ACT
Cuts at: 361 409 3134 361
Size: 48 2725 844
Fragments arranged by size:
2725 844 48
SfcI C'TryA_G
Cuts at: 33 660 1345 2338 2529 33
Size: 627 685 993 191 1121
Fragments arranged by size:
1121 993 685 627 191
SfoI GGC'GCC
Cuts at: 125 1049 1297 125
Size: 924 248 2445
Fragments arranged by size:
2445 924 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3617
SgrAI Cr'CCGG_yG
Cuts at: 120 120
Size: 3617
SmaI CCC'GGG
Cuts at: 639 639
Size: 3617
SmlI C'TyrA_G
Cuts at: 772 1687 2179 2441 772
Size: 915 492 262 1948
Fragments arranged by size:
1948 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3617
SphI G_CATG'C
Cuts at: 670 1170 670
Size: 500 3117
StyI C'CwwG_G
Cuts at: 107 751 1105 107
Size: 644 354 2619
Fragments arranged by size:
2619 644 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1200 1333 1792 2001 1200
Size: 133 459 209 2816
Fragments arranged by size:
2816 459 209 133
TatI w'GTAC_w
Cuts at: 359 407 3132 359
Size: 48 2725 844
Fragments arranged by size:
2725 844 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 235 267 3134 3166 235
Size: 32 2867 32 686
Fragments arranged by size:
2867 686 32 32
Tth111I GACn'n_nGTC
Cuts at: 1414 1414
Size: 3617
XbaI T'CTAG_A
Cuts at: 648 648
Size: 3617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1112 1112
Size: 3617
XmaI C'CCGG_G
Cuts at: 637 637
Size: 3617
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI