Restriction map for
pF1KE0900
(Circular) (Six-base) MAPSORT of: pF1KE0900.seq Check: 9672 from: 1 to: 13367
pF1KE0900 13367 bp
With 182 enzymes: SgfI *
December 21, 2016 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5640 5640
Size: 13367
AccI GT'mk_AC
Cuts at: 444 3635 4971 7729 8473 9933 10405 444
Size: 3191 1336 2758 744 1460 472 3406
Fragments arranged by size:
3406 3191 2758 1460 1336 744 472
Acc65I G'GTAC_C
Cuts at: 10383 10383
Size: 13367
AclI AA'CG_TT
Cuts at: 2597 13207 2597
Size: 10610 2757
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 847 1037 1196 1415 1727 2084 2765 3117
Size: 647 190 159 219 312 357 681 352
Cuts at: 3117 3821 5954 6518 7543 7820 9261 9689 11192
Size: 704 2133 564 1025 277 1441 428 1503
Cuts at: 11192 11624 12371 12639 200
Size: 432 747 268 928
Fragments arranged by size:
2133 1503 1441 1025 928 747 704 681
647 564 432 428 357 352 312 277
268 219 190 159
AflII C'TTAA_G
Cuts at: 4953 4953
Size: 13367
AflIII A'CryG_T
Cuts at: 555 2131 6871 7611 10719 11823 12800 555
Size: 1576 4740 740 3108 1104 977 1122
Fragments arranged by size:
4740 3108 1576 1122 1104 977 740
AgeI A'CCGG_T
Cuts at: 9222 11755 9222
Size: 2533 10834
AhdI GACnn_n'nnGTC
Cuts at: 3008 8348 3008
Size: 5340 8027
AleI CACnn'nnGTG
Cuts at: 9010 9010
Size: 13367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 8810 8825 8810
Size: 15 13352
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 416 448 7682 7714 416
Size: 32 7234 32 6069
Fragments arranged by size:
7234 6069 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2224 2743 7507 10672 12239 2224
Size: 519 4764 3165 1567 3352
Fragments arranged by size:
4764 3352 3165 1567 519
ApaLI G'TGCA_C
Cuts at: 8771 12137 8771
Size: 3366 10001
ApoI r'AATT_y
Cuts at: 146 406 513 921 1100 1437 1773 1815 3484
Size: 260 107 408 179 337 336 42 1669
Cuts at: 3484 4095 4111 4449 4494 4893 5103 5756 5891
Size: 611 16 338 45 399 210 653 135
Cuts at: 5891 6258 6352 6493 6903 6982 7149 7171 7903
Size: 367 94 141 410 79 167 22 732
Cuts at: 7903 7958 8029 8711 8722 9128 9236 9283 10059
Size: 55 71 682 11 406 108 47 776
Cuts at: 10059 10087 10097 10371 12720 12976 146
Size: 28 10 274 2349 256 537
Fragments arranged by size:
2349 1669 776 732 682 653 611 537
410 408 406 399 367 338 337 336
274 260 256 210 179 167 141 135
108 107 94 79 71 55 47 45
42 28 22 16 11 10
AseI AT'TA_AT
Cuts at: 20 3302 7454 20
Size: 3282 4152 5933
Fragments arranged by size:
5933 4152 3282
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 13367
AvaI C'yCGr_G
Cuts at: 10387 10387
Size: 13367
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 7088 7121 7088
Size: 33 13334
BamHI G'GATC_C
Cuts at: 118 672 7938 10392 118
Size: 554 7266 2454 3093
Fragments arranged by size:
7266 3093 2454 554
BanI G'GyrC_C
Cuts at: 4938 10021 10383 10797 11045 11080 4938
Size: 5083 362 414 248 35 7225
Fragments arranged by size:
7225 5083 414 362 248 35
BanII G_rGCy'C
Cuts at: 1326 6935 7917 8389 9269 10381 11411 1326
Size: 5609 982 472 880 1112 1030 3282
Fragments arranged by size:
5609 3282 1112 1030 982 880 472
BbeI G_GCGC'C
Cuts at: 10801 11049 10801
Size: 248 13119
BbsI GAAGACnn'nnnn_
Cuts at: 448 2772 6373 7549 8611 9752 448
Size: 2324 3601 1176 1062 1141 4063
Fragments arranged by size:
4063 3601 2324 1176 1141 1062
BbvCI CC'TCA_GC
Cuts at: 665 665
Size: 13367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 10120 10154 90
Size: 34 9996 34 3303
Fragments arranged by size:
9996 3303 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 11265 12026 13367 11265
Size: 761 1341 11265
Fragments arranged by size:
11265 1341 761
BclI T'GATC_A
Cuts at: 2391 4185 4458 10277 10887 2391
Size: 1794 273 5819 610 4871
Fragments arranged by size:
5819 4871 1794 610 273
BfrBI ATG'CAT
Cuts at: 5503 7074 9651 5503
Size: 1571 2577 9219
Fragments arranged by size:
9219 2577 1571
BglII A'GATC_T
Cuts at: 10882 12919 10882
Size: 2037 11330
BlpI GC'TnA_GC
Cuts at: 10479 10479
Size: 13367
Bme1580I G_kGCm'C
Cuts at: 8775 10992 11085 12141 8775
Size: 2217 93 1056 10001
Fragments arranged by size:
10001 2217 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1956 2320 4588 8292 10579 10660 10995 13139 1956
Size: 364 2268 3704 2287 81 335 2144 2184
Fragments arranged by size:
3704 2287 2268 2184 2144 364 335 81
BmtI G_CTAG'C
Cuts at: 4691 4691
Size: 13367
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3308 11734 3308
Size: 8426 4941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1568 6197 7022 7913 9466 9548 1568
Size: 4629 825 891 1553 82 5387
Fragments arranged by size:
5387 4629 1553 891 825 82
Bpu10I CC'TnA_GC
Cuts at: 665 4946 5112 8047 665
Size: 4281 166 2935 5985
Fragments arranged by size:
5985 4281 2935 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4277 8878 9948 10543 11422 11914 12212 4277
Size: 4601 1070 595 879 492 298 5432
Fragments arranged by size:
5432 4601 1070 879 595 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1298 4843 13071 1298
Size: 3545 8228 1594
Fragments arranged by size:
8228 3545 1594
BsaAI yAC'GTr
Cuts at: 1450 6208 7733 8770 10720 1450
Size: 4758 1525 1037 1950 4097
Fragments arranged by size:
4758 4097 1950 1525 1037
BsaBI GATnn'nnATC
Cuts at: 6 1196 3556 3593 10881 12918 6
Size: 1190 2360 37 7288 2037 455
Fragments arranged by size:
7288 2360 2037 1190 455 37
BsaHI Gr'CG_yC
Cuts at: 9078 10798 11046 11748 13244 9078
Size: 1720 248 702 1496 9201
Fragments arranged by size:
9201 1720 1496 702 248
BsaWI w'CCGG_w
Cuts at: 9222 10556 11077 11755 12029 12176 12587 9222
Size: 1334 521 678 274 147 411 10002
Fragments arranged by size:
10002 1334 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 653 683 1240 1270 7682 7712 8534 8564 653
Size: 30 557 30 6412 30 822 30 5456
Fragments arranged by size:
6412 5456 822 557 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2980 6941 10055 2980
Size: 3961 3114 6292
Fragments arranged by size:
6292 3961 3114
BseYI C'CCAG_C
Cuts at: 8847 10792 11550 12127 12623 8847
Size: 1945 758 577 496 9591
Fragments arranged by size:
9591 1945 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3299 8258 8831 9971 3299
Size: 4959 573 1140 6695
Fragments arranged by size:
6695 4959 1140 573
BsiEI CG_ry'CG
Cuts at: 104 10909 10955 12163 104
Size: 10805 46 1208 1308
Fragments arranged by size:
10805 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 734 6455 8775 10381 11159 11349 12141 734
Size: 5721 2320 1606 778 190 792 1960
Fragments arranged by size:
5721 2320 1960 1606 792 778 190
BsmI GAATG_Cn'
Cuts at: 1547 1839 2025 3586 3618 5853 7569 8841 1547
Size: 292 186 1561 32 2235 1716 1272 6073
Fragments arranged by size:
6073 2235 1716 1561 1272 292 186 32
Bsp1286I G_dGCh'C
Cuts at: 734 1326 6455 6935 7917 8389 8775 9269 10381
Size: 592 5129 480 982 472 386 494 1112
Cuts at: 10381 10992 11085 11159 11349 11411 12141 734
Size: 611 93 74 190 62 730 1960
Fragments arranged by size:
5129 1960 1112 982 730 611 592 494
480 472 386 190 93 74 62
BspEI T'CCGG_A
Cuts at: 12587 12587
Size: 13367
BspHI T'CATG_A
Cuts at: 774 813 2394 2456 2835 3903 12543 774
Size: 39 1581 62 379 1068 8640 1598
Fragments arranged by size:
8640 1598 1581 1068 379 62 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 252 1828 5640 5770 6490 10417 10631 10933 11314
Size: 1576 3812 130 720 3927 214 302 381
Cuts at: 11314 252
Size: 2305
Fragments arranged by size:
3927 3812 2305 1576 720 381 302 214
130
BsrBI CCG'CTC
Cuts at: 50 11659 11713 13197 13362 50
Size: 11609 54 1484 165 55
Fragments arranged by size:
11609 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 7749 8640 9633 11279 7749
Size: 891 993 1646 9837
Fragments arranged by size:
9837 1646 993 891
BsrFI r'CCGG_y
Cuts at: 9222 11365 11546 11755 9222
Size: 2143 181 209 10834
Fragments arranged by size:
10834 2143 209 181
BsrGI T'GTAC_A
Cuts at: 550 8401 9967 550
Size: 7851 1566 3950
Fragments arranged by size:
7851 3950 1566
BssSI C'ACGA_G
Cuts at: 6428 11638 11996 6428
Size: 5210 358 7799
Fragments arranged by size:
7799 5210 358
BstAPI GCAn_nnn'nTGC
Cuts at: 9657 10744 9657
Size: 1087 12280
BstBI TT'CG_AA
Cuts at: 11728 11728
Size: 13367
BstYI r'GATC_y
Cuts at: 1 118 672 2160 7938 8304 8936 9291 10392
Size: 117 554 1488 5778 366 632 355 1101
Cuts at: 10392 10859 10882 11217 11463 12475 12561 12573 12919
Size: 467 23 335 246 1012 86 12 346
Cuts at: 12919 1
Size: 449
Fragments arranged by size:
5778 1488 1101 1012 632 554 467 449
366 355 346 335 246 117 86 23
12
BstZ17I GTA'TAC
Cuts at: 7730 8474 7730
Size: 744 12623
Bsu36I CC'TnA_GG
Cuts at: 8024 8024
Size: 13367
BtgI C'CryG_G
Cuts at: 2549 2885 8960 9259 2549
Size: 336 6075 299 6657
Fragments arranged by size:
6657 6075 336 299
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 8510 11317 11498 11661 8510
Size: 2807 181 163 10216
Fragments arranged by size:
10216 2807 181 163
BtsI GCAGTG_nn'
Cuts at: 4128 8834 9716 10610 4128
Size: 4706 882 894 6885
Fragments arranged by size:
6885 4706 894 882
ClaI AT'CG_AT
Cuts at: 3622 3622
Size: 13367
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 8651 8686 8651
Size: 35 13332
DraI TTT'AAA
Cuts at: 294 930 1695 2579 3020 6156 6498 7338 10083
Size: 636 765 884 441 3136 342 840 2745
Cuts at: 10083 10366 294
Size: 283 3295
Fragments arranged by size:
3295 3136 2745 884 840 765 636 441
342 283
DraIII CAC_nnn'GTG
Cuts at: 5950 7523 5950
Size: 1573 11794
DrdI GACnn_nn'nnGTC
Cuts at: 188 452 3098 9858 11073 11931 188
Size: 264 2646 6760 1215 858 1624
Fragments arranged by size:
6760 2646 1624 1215 858 264
EaeI y'GGCC_r
Cuts at: 1795 4215 8255 9809 10952 11126 11517 11544 12889
Size: 2420 4040 1554 1143 174 391 27 1345
Cuts at: 12889 1795
Size: 2273
Fragments arranged by size:
4040 2420 2273 1554 1345 1143 391 174
27
EagI C'GGCC_G
Cuts at: 10952 10952
Size: 13367
EarI CTCTTCn'nnn_
Cuts at: 1511 2294 3178 3517 5562 6918 8870 9906 11390
Size: 783 884 339 2045 1356 1952 1036 1484
Cuts at: 11390 11600 1511
Size: 210 3278
Fragments arranged by size:
3278 2045 1952 1484 1356 1036 884 783
339 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 11575 11885 12031 13177 11575
Size: 310 146 1146 11765
Fragments arranged by size:
11765 1146 310 146
EcoICRI GAG'CTC
Cuts at: 10379 10379
Size: 13367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 847 1037 1196 1415 1568 1727 2084 2765
Size: 647 190 159 219 153 159 357 681
Cuts at: 2765 3117 3821 5954 6197 6518 7022 7543 7820
Size: 352 704 2133 243 321 504 521 277
Cuts at: 7820 7913 9261 9466 9548 9689 11192 11624 12371
Size: 93 1348 205 82 141 1503 432 747
Cuts at: 12371 12639 200
Size: 268 928
Fragments arranged by size:
2133 1503 1348 928 747 704 681 647
521 504 432 357 352 321 277 268
243 219 205 190 159 159 153 141
93 82
EcoNI CCTnn'n_nnAGG
Cuts at: 9436 9737 9436
Size: 301 13066
EcoO109I rG'GnC_Cy
Cuts at: 10506 10506
Size: 13367
EcoRI G'AATT_C
Cuts at: 921 1437 1815 4893 10059 10371 921
Size: 516 378 3078 5166 312 3917
Fragments arranged by size:
5166 3917 3078 516 378 312
EcoRV GAT'ATC
Cuts at: 5392 5392
Size: 13367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1441 2008 3905 6153 6640 11386 1441
Size: 567 1897 2248 487 4746 3422
Fragments arranged by size:
4746 3422 2248 1897 567 487
FspI TGC'GCA
Cuts at: 11148 11148
Size: 13367
HaeII r_GCGC'y
Cuts at: 10801 11049 12071 13050 10801
Size: 248 1022 979 11118
Fragments arranged by size:
11118 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1240 1272 1885 1917 1921 1953 2218 2250 2353
Size: 32 613 32 4 32 265 32 103
Cuts at: 2353 2385 2428 2460 2994 3007 3026 3039 3921
Size: 32 43 32 534 13 19 13 882
Cuts at: 3921 3953 5734 5766 7128 7160 8334 8366 8593
Size: 32 1781 32 1362 32 1174 32 227
Cuts at: 8593 8625 9747 9768 9779 9800 11209 11239 11241
Size: 32 1122 21 11 21 1409 30 2
Cuts at: 11241 11271 1240
Size: 30 3336
Fragments arranged by size:
3336 1781 1409 1362 1174 1122 882 613
534 265 227 103 43 32 32 32
32 32 32 32 32 32 32 32
30 30 21 21 19 13 13 11
4 2
HincII GTy'rAC
Cuts at: 4334 5209 9107 10406 4334
Size: 875 3898 1299 7295
Fragments arranged by size:
7295 3898 1299 875
HindIII A'AGCT_T
Cuts at: 5998 6803 7945 9643 9748 5998
Size: 805 1142 1698 105 9617
Fragments arranged by size:
9617 1698 1142 805 105
HpaI GTT'AAC
Cuts at: 4334 9107 4334
Size: 4773 8594
KasI G'GCGC_C
Cuts at: 10797 11045 10797
Size: 248 13119
KpnI G_GTAC'C
Cuts at: 10387 10387
Size: 13367
MluI A'CGCG_T
Cuts at: 7611 12800 7611
Size: 5189 8178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 8924 12038 12222 8924
Size: 3114 184 10069
Fragments arranged by size:
10069 3114 184
MscI TGG'CCA
Cuts at: 1797 4217 8257 9811 11128 1797
Size: 2420 4040 1554 1317 4036
Fragments arranged by size:
4040 4036 2420 1554 1317
MslI CAynn'nnrTG
Cuts at: 2399 4160 5030 5366 7193 9010 9665 11356 11483
Size: 1761 870 336 1827 1817 655 1691 127
Cuts at: 11483 2399
Size: 4283
Fragments arranged by size:
4283 1827 1817 1761 1691 870 655 336
127
MspA1I CmG'CkG
Cuts at: 327 375 4867 7507 7725 8152 10478 10792 11152
Size: 48 4492 2640 218 427 2326 314 360
Cuts at: 11152 12165 12410 327
Size: 1013 245 1284
Fragments arranged by size:
4492 2640 2326 1284 1013 427 360 314
245 218 48
NaeI GCC'GGC
Cuts at: 11548 11548
Size: 13367
NarI GG'CG_CC
Cuts at: 10798 11046 10798
Size: 248 13119
NcoI C'CATG_G
Cuts at: 2549 2885 8960 2549
Size: 336 6075 6956
Fragments arranged by size:
6956 6075 336
NdeI CA'TA_TG
Cuts at: 1676 4198 7990 9458 1676
Size: 2522 3792 1468 5585
Fragments arranged by size:
5585 3792 2522 1468
NgoMIV G'CCGG_C
Cuts at: 11546 11546
Size: 13367
NheI G'CTAG_C
Cuts at: 4687 4687
Size: 13367
NsiI A_TGCA'T
Cuts at: 5505 7076 9653 5505
Size: 1571 2577 9219
Fragments arranged by size:
9219 2577 1571
NspI r_CATG'y
Cuts at: 559 2135 2321 4304 4847 5343 6875 9664 10420
Size: 1576 186 1983 543 496 1532 2789 756
Cuts at: 10420 10920 11827 559
Size: 500 907 2099
Fragments arranged by size:
2789 2099 1983 1576 1532 907 756 543
500 496 186
PciI A'CATG_T
Cuts at: 555 2131 6871 11823 555
Size: 1576 4740 4952 2099
Fragments arranged by size:
4952 4740 2099 1576
PflMI CCAn_nnn'nTGG
Cuts at: 3625 8253 3625
Size: 4628 8739
PmeI GTTT'AAAC
Cuts at: 10366 10366
Size: 13367
PmlI CAC'GTG
Cuts at: 8770 8770
Size: 13367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2100 2132 7682 7714 12506 12538 2100
Size: 32 5550 32 4792 32 2929
Fragments arranged by size:
5550 4792 2929 32 32 32
PsiI TTA'TAA
Cuts at: 320 1907 2171 2591 7970 320
Size: 1587 264 420 5379 5717
Fragments arranged by size:
5717 5379 1587 420 264
PstI C_TGCA'G
Cuts at: 249 2246 2316 3867 5232 6487 10414 11099 249
Size: 1997 70 1551 1365 1255 3927 685 2517
Fragments arranged by size:
3927 2517 1997 1551 1365 1255 685 70
PvuI CG_AT'CG
Cuts at: 104 104
Size: 13367
PvuII CAG'CTG
Cuts at: 327 4867 7507 7725 8152 10792 11152 327
Size: 4540 2640 218 427 2640 360 2542
Fragments arranged by size:
4540 2640 2640 2542 427 360 218
RsrII CG'GwC_CG
Cuts at: 11562 11562
Size: 13367
SacI G_AGCT'C
Cuts at: 10381 10381
Size: 13367
SalI G'TCGA_C
Cuts at: 10404 10404
Size: 13367
SapI GCTCTTCn'nnn_
Cuts at: 1511 3178 5562 8870 9906 11390 11600 1511
Size: 1667 2384 3308 1036 1484 210 3278
Fragments arranged by size:
3308 3278 2384 1667 1484 1036 210
SbfI CC_TGCA'GG
Cuts at: 2246 10414 2246
Size: 8168 5199
ScaI AGT'ACT
Cuts at: 5274 8454 12884 5274
Size: 3180 4430 5757
Fragments arranged by size:
5757 4430 3180
SexAI A'CCwGG_T
Cuts at: 7161 7161
Size: 13367
SfcI C'TryA_G
Cuts at: 33 245 1820 2200 2242 2312 3863 5228 5276
Size: 212 1575 380 42 70 1551 1365 48
Cuts at: 5276 6483 7301 7977 10410 11095 12088 12279 33
Size: 1207 818 676 2433 685 993 191 1121
Fragments arranged by size:
2433 1575 1551 1365 1207 1121 993 818
685 676 380 212 191 70 48 42
SfoI GGC'GCC
Cuts at: 10799 11047 10799
Size: 248 13119
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 13367
SmaI CCC'GGG
Cuts at: 10389 10389
Size: 13367
SmlI C'TyrA_G
Cuts at: 4292 4953 8857 9927 10522 11437 11929 12191 4292
Size: 661 3904 1070 595 915 492 262 5468
Fragments arranged by size:
5468 3904 1070 915 661 595 492 262
SnaBI TAC'GTA
Cuts at: 6208 7733 6208
Size: 1525 11842
SpeI A'CTAG_T
Cuts at: 67 3226 4614 4765 5461 8230 67
Size: 3159 1388 151 696 2769 5204
Fragments arranged by size:
5204 3159 2769 1388 696 151
SphI G_CATG'C
Cuts at: 9664 10420 10920 9664
Size: 756 500 12111
Fragments arranged by size:
12111 756 500
SspI AAT'ATT
Cuts at: 347 1374 2544 3846 3999 4413 5198 7383 9612
Size: 1027 1170 1302 153 414 785 2185 2229
Cuts at: 9612 347
Size: 4102
Fragments arranged by size:
4102 2229 2185 1302 1170 1027 785 414
153
StuI AGG'CCT
Cuts at: 4822 9509 4822
Size: 4687 8680
StyI C'CwwG_G
Cuts at: 1259 2432 2549 2885 3257 8960 9917 10009 10501
Size: 1173 117 336 372 5703 957 92 492
Cuts at: 10501 10855 1259
Size: 354 3771
Fragments arranged by size:
5703 3771 1173 957 492 372 354 336
117 92
SwaI ATTT'AAAT
Cuts at: 6498 6498
Size: 13367
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 726 2339 8267 10053 10950 11083 11542 11751 726
Size: 1613 5928 1786 897 133 459 209 2342
Fragments arranged by size:
5928 2342 1786 1613 897 459 209 133
TatI w'GTAC_w
Cuts at: 550 2328 3526 4811 5022 5272 6283 8401 8452
Size: 1778 1198 1285 211 250 1011 2118 51
Cuts at: 8452 9700 9967 10264 12882 550
Size: 1248 267 297 2618 1035
Fragments arranged by size:
2618 2118 1778 1285 1248 1198 1035 1011
297 267 250 211 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 653 685 2239 2271 5634 5666 6602 6634 12884
Size: 32 1554 32 3363 32 936 32 6250
Cuts at: 12884 12916 653
Size: 32 1104
Fragments arranged by size:
6250 3363 1554 1104 936 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 11164 11164
Size: 13367
XbaI T'CTAG_A
Cuts at: 2119 2464 5829 6400 7954 10398 2119
Size: 345 3365 571 1554 2444 5088
Fragments arranged by size:
5088 3365 2444 1554 571 345
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5914 10862 5914
Size: 4948 8419
XmaI C'CCGG_G
Cuts at: 10387 10387
Size: 13367
XmnI GAAnn'nnTTC
Cuts at: 2046 3141 5142 2046
Size: 1095 2001 10271
Fragments arranged by size:
10271 2001 1095
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI
PciI PflMI PmeI PmlI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI ApaI AscI AvrII BglI BmgBI BsiWI
BsmBI BssHII BstEII BstXI FseI FspAI MfeI NotI
NruI PacI PasI PfoI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SrfI XhoI ZraI