Restriction map for
pF1KE0850
(Circular) (Six-base) MAPSORT of: pF1KE0850.seq Check: 3702 from: 1 to: 4064
pF1KE0850 4064 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AccI GT'mk_AC
Cuts at: 1102 1102
Size: 4064
Acc65I G'GTAC_C
Cuts at: 1080 1080
Size: 4064
AclI AA'CG_TT
Cuts at: 3904 3904
Size: 4064
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 854 1889 2321 3068 3336 854
Size: 1035 432 747 268 1582
Fragments arranged by size:
1582 1035 747 432 268
AflIII A'CryG_T
Cuts at: 1416 2520 3497 1416
Size: 1104 977 1983
Fragments arranged by size:
1983 1104 977
AgeI A'CCGG_T
Cuts at: 2452 2452
Size: 4064
AlwNI CAG_nnn'CTG
Cuts at: 1369 2936 1369
Size: 1567 2497
ApaLI G'TGCA_C
Cuts at: 766 2834 766
Size: 2068 1996
ApoI r'AATT_y
Cuts at: 136 1068 3417 3673 136
Size: 932 2349 256 527
Fragments arranged by size:
2349 932 527 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4064
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
AvaI C'yCGr_G
Cuts at: 586 1084 586
Size: 498 3566
BamHI G'GATC_C
Cuts at: 1089 1089
Size: 4064
BanI G'GyrC_C
Cuts at: 1080 1494 1742 1777 1080
Size: 414 248 35 3367
Fragments arranged by size:
3367 414 248 35
BanII G_rGCy'C
Cuts at: 166 611 1078 2108 166
Size: 445 467 1030 2122
Fragments arranged by size:
2122 1030 467 445
BbeI G_GCGC'C
Cuts at: 1498 1746 1498
Size: 248 3816
BbsI GAAGACnn'nnnn_
Cuts at: 414 823 414
Size: 409 3655
BbvCI CC'TCA_GC
Cuts at: 787 787
Size: 4064
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 567 601 90
Size: 34 443 34 3553
Fragments arranged by size:
3553 443 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1962 2723 4064 1962
Size: 761 1341 1962
Fragments arranged by size:
1962 1341 761
BclI T'GATC_A
Cuts at: 569 1584 569
Size: 1015 3049
BglII A'GATC_T
Cuts at: 1579 3616 1579
Size: 2037 2027
BlpI GC'TnA_GC
Cuts at: 1176 1176
Size: 4064
Bme1580I G_kGCm'C
Cuts at: 770 1023 1689 1782 2838 770
Size: 253 666 93 1056 1996
Fragments arranged by size:
1996 1056 666 253 93
BmrI ACTGGGnnnn_n'
Cuts at: 1276 1357 1692 3836 1276
Size: 81 335 2144 1504
Fragments arranged by size:
2144 1504 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 202 2431 202
Size: 2229 1835
Bpu10I CC'TnA_GC
Cuts at: 112 787 112
Size: 675 3389
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 2119 2611 2909 1240
Size: 879 492 298 2395
Fragments arranged by size:
2395 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3768 3768
Size: 4064
BsaAI yAC'GTr
Cuts at: 1417 1417
Size: 4064
BsaBI GATnn'nnATC
Cuts at: 6 355 1578 3615 6
Size: 349 1223 2037 455
Fragments arranged by size:
2037 1223 455 349
BsaHI Gr'CG_yC
Cuts at: 1495 1743 2445 3941 1495
Size: 248 702 1496 1618
Fragments arranged by size:
1618 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1253 1774 2452 2726 2873 3284 1253
Size: 521 678 274 147 411 2033
Fragments arranged by size:
2033 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 370 400 370
Size: 30 4034
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 789 792 789
Size: 3 4061
BseYI C'CCAG_C
Cuts at: 1043 1489 2247 2824 3320 1043
Size: 446 758 577 496 1787
Fragments arranged by size:
1787 758 577 496 446
BsiEI CG_ry'CG
Cuts at: 104 1606 1652 2860 104
Size: 1502 46 1208 1308
Fragments arranged by size:
1502 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 166 749 770 1078 1856 2046 2838 166
Size: 583 21 308 778 190 792 1392
Fragments arranged by size:
1392 792 778 583 308 190 21
Bsp1286I G_dGCh'C
Cuts at: 166 611 749 770 1023 1078 1689 1782 1856
Size: 445 138 21 253 55 611 93 74
Cuts at: 1856 2046 2108 2838 166
Size: 190 62 730 1392
Fragments arranged by size:
1392 730 611 445 253 190 138 93
74 62 55 21
BspEI T'CCGG_A
Cuts at: 3284 3284
Size: 4064
BspHI T'CATG_A
Cuts at: 3240 3240
Size: 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1114 1328 1630 2011 1114
Size: 214 302 381 3167
Fragments arranged by size:
3167 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2356 2410 3894 4059 50
Size: 2306 54 1484 165 55
Fragments arranged by size:
2306 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 181 1976 181
Size: 1795 2269
BsrFI r'CCGG_y
Cuts at: 2062 2243 2452 2062
Size: 181 209 3674
Fragments arranged by size:
3674 209 181
BsrGI T'GTAC_A
Cuts at: 941 941
Size: 4064
BssSI C'ACGA_G
Cuts at: 145 176 364 2335 2693 145
Size: 31 188 1971 358 1516
Fragments arranged by size:
1971 1516 358 188 31
BstAPI GCAn_nnn'nTGC
Cuts at: 709 1441 709
Size: 732 3332
BstBI TT'CG_AA
Cuts at: 2425 2425
Size: 4064
BstYI r'GATC_y
Cuts at: 1 1089 1556 1579 1914 2160 3172 3258 3270
Size: 1088 467 23 335 246 1012 86 12
Cuts at: 3270 3616 1
Size: 346 449
Fragments arranged by size:
1088 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 804 804
Size: 4064
BtgI C'CryG_G
Cuts at: 221 740 1010 221
Size: 519 270 3275
Fragments arranged by size:
3275 519 270
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2014 2195 2358 2014
Size: 181 163 3720
Fragments arranged by size:
3720 181 163
BtsI GCAGTG_nn'
Cuts at: 1307 1307
Size: 4064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 335 370 335
Size: 35 4029
DraI TTT'AAA
Cuts at: 1063 1063
Size: 4064
DraIII CAC_nnn'GTG
Cuts at: 366 366
Size: 4064
DrdI GACnn_nn'nnGTC
Cuts at: 382 1770 2628 382
Size: 1388 858 1818
Fragments arranged by size:
1818 1388 858
EaeI y'GGCC_r
Cuts at: 224 300 437 776 1649 1823 2214 2241 3586
Size: 76 137 339 873 174 391 27 1345
Cuts at: 3586 224
Size: 702
Fragments arranged by size:
1345 873 702 391 339 174 137 76
27
EagI C'GGCC_G
Cuts at: 1649 1649
Size: 4064
EarI CTCTTCn'nnn_
Cuts at: 202 2087 2297 202
Size: 1885 210 1969
Fragments arranged by size:
1969 1885 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2272 2582 2728 3874 2272
Size: 310 146 1146 2462
Fragments arranged by size:
2462 1146 310 146
EcoICRI GAG'CTC
Cuts at: 164 1076 164
Size: 912 3152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 854 1889 2321 3068 3336 854
Size: 1035 432 747 268 1582
Fragments arranged by size:
1582 1035 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 651 681 1203 651
Size: 30 522 3512
Fragments arranged by size:
3512 522 30
EcoRI G'AATT_C
Cuts at: 1068 1068
Size: 4064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 4064
FspI TGC'GCA
Cuts at: 1845 1845
Size: 4064
HaeII r_GCGC'y
Cuts at: 1498 1746 2768 3747 1498
Size: 248 1022 979 1815
Fragments arranged by size:
1815 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1906 1936 1938 1968 1906
Size: 30 2 30 4002
Fragments arranged by size:
4002 30 30 2
HincII GTy'rAC
Cuts at: 1103 1103
Size: 4064
HindIII A'AGCT_T
Cuts at: 636 636
Size: 4064
KasI G'GCGC_C
Cuts at: 1494 1742 1494
Size: 248 3816
KpnI G_GTAC'C
Cuts at: 1084 1084
Size: 4064
MluI A'CGCG_T
Cuts at: 3497 3497
Size: 4064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2735 2919 2735
Size: 184 3880
MscI TGG'CCA
Cuts at: 226 778 1825 226
Size: 552 1047 2465
Fragments arranged by size:
2465 1047 552
MslI CAynn'nnrTG
Cuts at: 226 2053 2180 226
Size: 1827 127 2110
Fragments arranged by size:
2110 1827 127
MspA1I CmG'CkG
Cuts at: 1175 1489 1849 2862 3107 1175
Size: 314 360 1013 245 2132
Fragments arranged by size:
2132 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2245 2245
Size: 4064
NarI GG'CG_CC
Cuts at: 1495 1743 1495
Size: 248 3816
NcoI C'CATG_G
Cuts at: 221 740 1010 221
Size: 519 270 3275
Fragments arranged by size:
3275 519 270
NgoMIV G'CCGG_C
Cuts at: 2243 2243
Size: 4064
NspI r_CATG'y
Cuts at: 1117 1617 2524 1117
Size: 500 907 2657
Fragments arranged by size:
2657 907 500
PciI A'CATG_T
Cuts at: 2520 2520
Size: 4064
PflMI CCAn_nnn'nTGG
Cuts at: 366 366
Size: 4064
PfoI T'CCnGG_A
Cuts at: 256 314 256
Size: 58 4006
PmeI GTTT'AAAC
Cuts at: 1063 1063
Size: 4064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 4032
PpuMI rG'GwC_Cy
Cuts at: 681 681
Size: 4064
PshAI GACnn'nnGTC
Cuts at: 850 850
Size: 4064
PstI C_TGCA'G
Cuts at: 1111 1796 1111
Size: 685 3379
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4064
PvuII CAG'CTG
Cuts at: 1489 1849 1489
Size: 360 3704
RsrII CG'GwC_CG
Cuts at: 2259 2259
Size: 4064
SacI G_AGCT'C
Cuts at: 166 1078 166
Size: 912 3152
SalI G'TCGA_C
Cuts at: 1101 1101
Size: 4064
SanDI GG'GwC_CC
Cuts at: 681 681
Size: 4064
SapI GCTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
SbfI CC_TGCA'GG
Cuts at: 1111 1111
Size: 4064
ScaI AGT'ACT
Cuts at: 3581 3581
Size: 4064
SfcI C'TryA_G
Cuts at: 33 210 1107 1792 2785 2976 33
Size: 177 897 685 993 191 1121
Fragments arranged by size:
1121 993 897 685 191 177
SfoI GGC'GCC
Cuts at: 1496 1744 1496
Size: 248 3816
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
SmaI CCC'GGG
Cuts at: 1086 1086
Size: 4064
SmlI C'TyrA_G
Cuts at: 586 1219 2134 2626 2888 586
Size: 633 915 492 262 1762
Fragments arranged by size:
1762 915 633 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4064
SphI G_CATG'C
Cuts at: 1117 1617 1117
Size: 500 3564
StyI C'CwwG_G
Cuts at: 221 740 1010 1198 1552 221
Size: 519 270 188 354 2733
Fragments arranged by size:
2733 519 354 270 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1647 1780 2239 2448 1647
Size: 133 459 209 3263
Fragments arranged by size:
3263 459 209 133
TatI w'GTAC_w
Cuts at: 287 413 941 3579 287
Size: 126 528 2638 772
Fragments arranged by size:
2638 772 528 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 370 402 3581 3613 370
Size: 32 3179 32 821
Fragments arranged by size:
3179 821 32 32
Tth111I GACn'n_nGTC
Cuts at: 1861 1861
Size: 4064
XbaI T'CTAG_A
Cuts at: 1095 1095
Size: 4064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 904 1559 904
Size: 655 3409
XhoI C'TCGA_G
Cuts at: 586 586
Size: 4064
XmaI C'CCGG_G
Cuts at: 1084 1084
Size: 4064
XmnI GAAnn'nnTTC
Cuts at: 820 820
Size: 4064
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BfrBI BglI BmgBI BmtI
BpmI BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I ClaI EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI