Restriction map for
pF1KE0804
(Circular) (Six-base) MAPSORT of: pF1KE0804.seq Check: 1689 from: 1 to: 3980
pF1KE0804 3980 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1018 1018
Size: 3980
Acc65I G'GTAC_C
Cuts at: 996 996
Size: 3980
AclI AA'CG_TT
Cuts at: 3820 3820
Size: 3980
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 1805 2237 2984 3252 387
Size: 1418 432 747 268 1115
Fragments arranged by size:
1418 1115 747 432 268
AfeI AGC'GCT
Cuts at: 415 415
Size: 3980
AflIII A'CryG_T
Cuts at: 1332 2436 3413 1332
Size: 1104 977 1899
Fragments arranged by size:
1899 1104 977
AgeI A'CCGG_T
Cuts at: 186 2368 186
Size: 2182 1798
AlwNI CAG_nnn'CTG
Cuts at: 774 957 1285 2852 774
Size: 183 328 1567 1902
Fragments arranged by size:
1902 1567 328 183
ApaI G_GGCC'C
Cuts at: 241 918 241
Size: 677 3303
ApaLI G'TGCA_C
Cuts at: 2750 2750
Size: 3980
ApoI r'AATT_y
Cuts at: 984 3333 3589 984
Size: 2349 256 1375
Fragments arranged by size:
2349 1375 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3980
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3980
AvaI C'yCGr_G
Cuts at: 178 858 877 1000 178
Size: 680 19 123 3158
Fragments arranged by size:
3158 680 123 19
BamHI G'GATC_C
Cuts at: 1005 1005
Size: 3980
BanI G'GyrC_C
Cuts at: 331 928 940 996 1410 1658 1693 331
Size: 597 12 56 414 248 35 2618
Fragments arranged by size:
2618 597 414 248 56 35 12
BanII G_rGCy'C
Cuts at: 241 386 918 994 2024 241
Size: 145 532 76 1030 2197
Fragments arranged by size:
2197 1030 532 145 76
BbeI G_GCGC'C
Cuts at: 335 932 1414 1662 335
Size: 597 482 248 2653
Fragments arranged by size:
2653 597 482 248
BbsI GAAGACnn'nnnn_
Cuts at: 144 744 144
Size: 600 3380
BbvCI CC'TCA_GC
Cuts at: 895 895
Size: 3980
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 922 956 922
Size: 34 3946
BciVI GTATCCnnnnn_n'
Cuts at: 1878 2639 3980 1878
Size: 761 1341 1878
Fragments arranged by size:
1878 1341 761
BclI T'GATC_A
Cuts at: 1500 1500
Size: 3980
BglI GCCn_nnn'nGGC
Cuts at: 302 634 302
Size: 332 3648
BglII A'GATC_T
Cuts at: 1495 3532 1495
Size: 2037 1943
BlpI GC'TnA_GC
Cuts at: 1092 1092
Size: 3980
Bme1580I G_kGCm'C
Cuts at: 241 565 918 1605 1698 2754 241
Size: 324 353 687 93 1056 1467
Fragments arranged by size:
1467 1056 687 353 324 93
BmrI ACTGGGnnnn_n'
Cuts at: 1192 1273 1608 3752 1192
Size: 81 335 2144 1420
Fragments arranged by size:
2144 1420 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2347 2347
Size: 3980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 780 150
Size: 630 3350
Bpu10I CC'TnA_GC
Cuts at: 317 895 317
Size: 578 3402
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1156 2035 2527 2825 1156
Size: 879 492 298 2311
Fragments arranged by size:
2311 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 973 3684 973
Size: 2711 1269
BsaAI yAC'GTr
Cuts at: 588 1333 588
Size: 745 3235
BsaBI GATnn'nnATC
Cuts at: 6 1494 3531 6
Size: 1488 2037 455
Fragments arranged by size:
2037 1488 455
BsaHI Gr'CG_yC
Cuts at: 332 929 1411 1659 2361 3857 332
Size: 597 482 248 702 1496 455
Fragments arranged by size:
1496 702 597 482 455 248
BsaWI w'CCGG_w
Cuts at: 186 1169 1690 2368 2642 2789 3200 186
Size: 983 521 678 274 147 411 966
Fragments arranged by size:
983 966 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 823 853 823
Size: 30 3950
BseYI C'CCAG_C
Cuts at: 936 1405 2163 2740 3236 936
Size: 469 758 577 496 1680
Fragments arranged by size:
1680 758 577 496 469
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 244
Size: 3980
BsiEI CG_ry'CG
Cuts at: 104 476 515 1522 1568 2776 104
Size: 372 39 1007 46 1208 1308
Fragments arranged by size:
1308 1208 1007 372 46 39
BsiHKAI G_wGCw'C
Cuts at: 209 359 994 1772 1962 2754 209
Size: 150 635 778 190 792 1435
Fragments arranged by size:
1435 792 778 635 190 150
Bsp1286I G_dGCh'C
Cuts at: 209 241 359 386 565 918 994 1605 1698
Size: 32 118 27 179 353 76 611 93
Cuts at: 1698 1772 1962 2024 2754 209
Size: 74 190 62 730 1435
Fragments arranged by size:
1435 730 611 353 190 179 118 93
76 74 62 32 27
BspEI T'CCGG_A
Cuts at: 3200 3200
Size: 3980
BspHI T'CATG_A
Cuts at: 3156 3156
Size: 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 263 673 1030 1244 1546 1927 263
Size: 410 357 214 302 381 2316
Fragments arranged by size:
2316 410 381 357 302 214
BsrBI CCG'CTC
Cuts at: 50 2272 2326 3810 3975 50
Size: 2222 54 1484 165 55
Fragments arranged by size:
2222 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1892 1892
Size: 3980
BsrFI r'CCGG_y
Cuts at: 186 455 510 1978 2159 2368 186
Size: 269 55 1468 181 209 1798
Fragments arranged by size:
1798 1468 269 209 181 55
BssHII G'CGCG_C
Cuts at: 713 713
Size: 3980
BssSI C'ACGA_G
Cuts at: 2251 2609 2251
Size: 358 3622
BstAPI GCAn_nnn'nTGC
Cuts at: 774 1357 774
Size: 583 3397
BstBI TT'CG_AA
Cuts at: 2341 2341
Size: 3980
BstEII G'GTnAC_C
Cuts at: 660 660
Size: 3980
BstXI CCAn_nnnn'nTGG
Cuts at: 819 819
Size: 3980
BstYI r'GATC_y
Cuts at: 1 1005 1472 1495 1830 2076 3088 3174 3186
Size: 1004 467 23 335 246 1012 86 12
Cuts at: 3186 3532 1
Size: 346 449
Fragments arranged by size:
1012 1004 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 311 682 962 311
Size: 371 280 3329
Fragments arranged by size:
3329 371 280
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1930 2111 2274 1930
Size: 181 163 3636
Fragments arranged by size:
3636 181 163
BtsI GCAGTG_nn'
Cuts at: 1223 1223
Size: 3980
DraI TTT'AAA
Cuts at: 979 979
Size: 3980
DrdI GACnn_nn'nnGTC
Cuts at: 148 1686 2544 148
Size: 1538 858 1584
Fragments arranged by size:
1584 1538 858
EaeI y'GGCC_r
Cuts at: 266 473 512 590 809 1565 1739 2130 2157
Size: 207 39 78 219 756 174 391 27
Cuts at: 2157 3502 266
Size: 1345 744
Fragments arranged by size:
1345 756 744 391 219 207 174 78
39 27
EagI C'GGCC_G
Cuts at: 473 512 1565 473
Size: 39 1053 2888
Fragments arranged by size:
2888 1053 39
EarI CTCTTCn'nnn_
Cuts at: 436 733 2003 2213 436
Size: 297 1270 210 2203
Fragments arranged by size:
2203 1270 297 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2188 2498 2644 3790 2188
Size: 310 146 1146 2378
Fragments arranged by size:
2378 1146 310 146
EcoICRI GAG'CTC
Cuts at: 992 992
Size: 3980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 387 780 1805 2237 2984 3252 150
Size: 237 393 1025 432 747 268 878
Fragments arranged by size:
1025 878 747 432 393 268 237
EcoNI CCTnn'n_nnAGG
Cuts at: 922 922
Size: 3980
EcoO109I rG'GnC_Cy
Cuts at: 237 238 914 915 1119 237
Size: 1 676 1 204 3098
Fragments arranged by size:
3098 676 204 1 1
EcoRI G'AATT_C
Cuts at: 984 984
Size: 3980
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 258 1999 258
Size: 1741 2239
FspI TGC'GCA
Cuts at: 550 1761 550
Size: 1211 2769
HaeII r_GCGC'y
Cuts at: 335 417 932 1414 1662 2684 3663 335
Size: 82 515 482 248 1022 979 652
Fragments arranged by size:
1022 979 652 515 482 248 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1822 1852 1854 1884 1822
Size: 30 2 30 3918
Fragments arranged by size:
3918 30 30 2
HincII GTy'rAC
Cuts at: 831 1019 831
Size: 188 3792
KasI G'GCGC_C
Cuts at: 331 928 1410 1658 331
Size: 597 482 248 2653
Fragments arranged by size:
2653 597 482 248
KpnI G_GTAC'C
Cuts at: 1000 1000
Size: 3980
MluI A'CGCG_T
Cuts at: 3413 3413
Size: 3980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 449 2651 2835 449
Size: 2202 184 1594
Fragments arranged by size:
2202 1594 184
MscI TGG'CCA
Cuts at: 592 811 1741 592
Size: 219 930 2831
Fragments arranged by size:
2831 930 219
MslI CAynn'nnrTG
Cuts at: 1969 2096 1969
Size: 127 3853
MspA1I CmG'CkG
Cuts at: 522 684 807 894 1091 1405 1765 2778 3023
Size: 162 123 87 197 314 360 1013 245
Cuts at: 3023 522
Size: 1479
Fragments arranged by size:
1479 1013 360 314 245 197 162 123
87
NaeI GCC'GGC
Cuts at: 457 512 2161 457
Size: 55 1649 2276
Fragments arranged by size:
2276 1649 55
NarI GG'CG_CC
Cuts at: 332 929 1411 1659 332
Size: 597 482 248 2653
Fragments arranged by size:
2653 597 482 248
NcoI C'CATG_G
Cuts at: 962 962
Size: 3980
NgoMIV G'CCGG_C
Cuts at: 455 510 2159 455
Size: 55 1649 2276
Fragments arranged by size:
2276 1649 55
NspI r_CATG'y
Cuts at: 1033 1533 2440 1033
Size: 500 907 2573
Fragments arranged by size:
2573 907 500
PasI CC'CwG_GG
Cuts at: 327 918 327
Size: 591 3389
PciI A'CATG_T
Cuts at: 2436 2436
Size: 3980
PmeI GTTT'AAAC
Cuts at: 979 979
Size: 3980
PmlI CAC'GTG
Cuts at: 588 588
Size: 3980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3119 3151 3119
Size: 32 3948
PspOMI G'GGCC_C
Cuts at: 237 914 237
Size: 677 3303
PstI C_TGCA'G
Cuts at: 770 1027 1712 770
Size: 257 685 3038
Fragments arranged by size:
3038 685 257
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3980
PvuII CAG'CTG
Cuts at: 1405 1765 1405
Size: 360 3620
RsrII CG'GwC_CG
Cuts at: 2175 2175
Size: 3980
SacI G_AGCT'C
Cuts at: 994 994
Size: 3980
SacII CC_GC'GG
Cuts at: 685 685
Size: 3980
SalI G'TCGA_C
Cuts at: 1017 1017
Size: 3980
SapI GCTCTTCn'nnn_
Cuts at: 733 2003 2213 733
Size: 1270 210 2500
Fragments arranged by size:
2500 1270 210
SbfI CC_TGCA'GG
Cuts at: 1027 1027
Size: 3980
ScaI AGT'ACT
Cuts at: 3497 3497
Size: 3980
SexAI A'CCwGG_T
Cuts at: 601 601
Size: 3980
SfcI C'TryA_G
Cuts at: 33 766 1023 1708 2701 2892 33
Size: 733 257 685 993 191 1121
Fragments arranged by size:
1121 993 733 685 257 191
SfoI GGC'GCC
Cuts at: 333 930 1412 1660 333
Size: 597 482 248 2653
Fragments arranged by size:
2653 597 482 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3980
SmaI CCC'GGG
Cuts at: 879 1002 879
Size: 123 3857
SmlI C'TyrA_G
Cuts at: 1135 2050 2542 2804 1135
Size: 915 492 262 2311
Fragments arranged by size:
2311 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3980
SphI G_CATG'C
Cuts at: 1033 1533 1033
Size: 500 3480
StyI C'CwwG_G
Cuts at: 962 1114 1468 962
Size: 152 354 3474
Fragments arranged by size:
3474 354 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 648 738 928 1563 1696 2155 2364 648
Size: 90 190 635 133 459 209 2264
Fragments arranged by size:
2264 635 459 209 190 133 90
TatI w'GTAC_w
Cuts at: 3495 3495
Size: 3980
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3497 3529 3497
Size: 32 3948
Tth111I GACn'n_nGTC
Cuts at: 1777 1777
Size: 3980
XbaI T'CTAG_A
Cuts at: 1011 1011
Size: 3980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1475 1475
Size: 3980
XmaI C'CCGG_G
Cuts at: 877 1000 877
Size: 123 3857
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PmlI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BfrBI BmgBI BmtI BseRI BsiWI BsmI
BsmBI BsrGI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PpuMI PshAI PsiI
PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI