Restriction map for
pF1KE0802
(Circular) (Six-base) MAPSORT of: pF1KE0802.seq Check: 7510 from: 1 to: 4061
pF1KE0802 4061 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 986 1099 986
Size: 113 3948
Acc65I G'GTAC_C
Cuts at: 1077 1077
Size: 4061
AclI AA'CG_TT
Cuts at: 3901 3901
Size: 4061
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1886 2318 3065 3333 1886
Size: 432 747 268 2614
Fragments arranged by size:
2614 747 432 268
AflIII A'CryG_T
Cuts at: 832 843 1413 2517 3494 832
Size: 11 570 1104 977 1399
Fragments arranged by size:
1399 1104 977 570 11
AgeI A'CCGG_T
Cuts at: 2449 2449
Size: 4061
AleI CACnn'nnGTG
Cuts at: 848 848
Size: 4061
AlwNI CAG_nnn'CTG
Cuts at: 1366 2933 1366
Size: 1567 2494
ApaLI G'TGCA_C
Cuts at: 895 2831 895
Size: 1936 2125
ApoI r'AATT_y
Cuts at: 113 1065 3414 3670 113
Size: 952 2349 256 504
Fragments arranged by size:
2349 952 504 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4061
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
AvaI C'yCGr_G
Cuts at: 1081 1081
Size: 4061
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 277 310 277
Size: 33 4028
BamHI G'GATC_C
Cuts at: 1086 1086
Size: 4061
BanI G'GyrC_C
Cuts at: 1015 1077 1491 1739 1774 1015
Size: 62 414 248 35 3302
Fragments arranged by size:
3302 414 248 62 35
BanII G_rGCy'C
Cuts at: 773 1075 2105 773
Size: 302 1030 2729
Fragments arranged by size:
2729 1030 302
BbeI G_GCGC'C
Cuts at: 1495 1743 1495
Size: 248 3813
BciVI GTATCCnnnnn_n'
Cuts at: 1959 2720 4061 1959
Size: 761 1341 1959
Fragments arranged by size:
1959 1341 761
BclI T'GATC_A
Cuts at: 1581 1581
Size: 4061
BglI GCCn_nnn'nGGC
Cuts at: 313 940 313
Size: 627 3434
BglII A'GATC_T
Cuts at: 1576 3613 1576
Size: 2037 2024
BlpI GC'TnA_GC
Cuts at: 1173 1173
Size: 4061
Bme1580I G_kGCm'C
Cuts at: 899 1686 1779 2835 899
Size: 787 93 1056 2125
Fragments arranged by size:
2125 1056 787 93
BmgBI CAC'GTC
Cuts at: 258 846 258
Size: 588 3473
BmrI ACTGGGnnnn_n'
Cuts at: 1273 1354 1689 3833 1273
Size: 81 335 2144 1501
Fragments arranged by size:
2144 1501 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 824 196
Size: 628 3433
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 2116 2608 2906 1237
Size: 879 492 298 2392
Fragments arranged by size:
2392 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3765 3765
Size: 4061
BsaAI yAC'GTr
Cuts at: 1414 1414
Size: 4061
BsaBI GATnn'nnATC
Cuts at: 6 220 757 1575 3612 6
Size: 214 537 818 2037 455
Fragments arranged by size:
2037 818 537 455 214
BsaHI Gr'CG_yC
Cuts at: 610 1492 1740 2442 3938 610
Size: 882 248 702 1496 733
Fragments arranged by size:
1496 882 733 702 248
BsaWI w'CCGG_w
Cuts at: 1250 1771 2449 2723 2870 3281 1250
Size: 521 678 274 147 411 2030
Fragments arranged by size:
2030 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1486 2244 2821 3317 1486
Size: 758 577 496 2230
Fragments arranged by size:
2230 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 641 641
Size: 4061
BsiEI CG_ry'CG
Cuts at: 104 1603 1649 2857 104
Size: 1499 46 1208 1308
Fragments arranged by size:
1499 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 188 899 1075 1853 2043 2835 188
Size: 711 176 778 190 792 1414
Fragments arranged by size:
1414 792 778 711 190 176
BsmI GAATG_Cn'
Cuts at: 1034 1034
Size: 4061
Bsp1286I G_dGCh'C
Cuts at: 188 773 899 1075 1686 1779 1853 2043 2105
Size: 585 126 176 611 93 74 190 62
Cuts at: 2105 2835 188
Size: 730 1414
Fragments arranged by size:
1414 730 611 585 190 176 126 93
74 62
BspEI T'CCGG_A
Cuts at: 3281 3281
Size: 4061
BspHI T'CATG_A
Cuts at: 3237 3237
Size: 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1111 1325 1627 2008 1111
Size: 214 302 381 3164
Fragments arranged by size:
3164 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2353 2407 3891 4056 50
Size: 2303 54 1484 165 55
Fragments arranged by size:
2303 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1973 1973
Size: 4061
BsrFI r'CCGG_y
Cuts at: 2059 2240 2449 2059
Size: 181 209 3671
Fragments arranged by size:
3671 209 181
BssSI C'ACGA_G
Cuts at: 2332 2690 2332
Size: 358 3703
BstAPI GCAn_nnn'nTGC
Cuts at: 1438 1438
Size: 4061
BstBI TT'CG_AA
Cuts at: 2422 2422
Size: 4061
BstXI CCAn_nnnn'nTGG
Cuts at: 475 475
Size: 4061
BstYI r'GATC_y
Cuts at: 1 1007 1086 1553 1576 1911 2157 3169 3255
Size: 1006 79 467 23 335 246 1012 86
Cuts at: 3255 3267 3613 1
Size: 12 346 449
Fragments arranged by size:
1012 1006 467 449 346 335 246 86
79 23 12
BtgI C'CryG_G
Cuts at: 1011 1011
Size: 4061
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2011 2192 2355 2011
Size: 181 163 3717
Fragments arranged by size:
3717 181 163
BtsI GCAGTG_nn'
Cuts at: 527 1304 527
Size: 777 3284
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 816 851 816
Size: 35 4026
DraI TTT'AAA
Cuts at: 1060 1060
Size: 4061
DrdI GACnn_nn'nnGTC
Cuts at: 1767 2625 1767
Size: 858 3203
EaeI y'GGCC_r
Cuts at: 320 465 625 941 1646 1820 2211 2238 3583
Size: 145 160 316 705 174 391 27 1345
Cuts at: 3583 320
Size: 798
Fragments arranged by size:
1345 798 705 391 316 174 160 145
27
EagI C'GGCC_G
Cuts at: 1646 1646
Size: 4061
EarI CTCTTCn'nnn_
Cuts at: 304 961 2084 2294 304
Size: 657 1123 210 2071
Fragments arranged by size:
2071 1123 657 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2269 2579 2725 3871 2269
Size: 310 146 1146 2459
Fragments arranged by size:
2459 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1073 1073
Size: 4061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 824 1886 2318 3065 3333 196
Size: 628 1062 432 747 268 924
Fragments arranged by size:
1062 924 747 628 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1200 1200
Size: 4061
EcoRI G'AATT_C
Cuts at: 1065 1065
Size: 4061
EcoRV GAT'ATC
Cuts at: 197 197
Size: 4061
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 424 2080 424
Size: 1656 2405
FspI TGC'GCA
Cuts at: 1842 1842
Size: 4061
HaeII r_GCGC'y
Cuts at: 1495 1743 2765 3744 1495
Size: 248 1022 979 1812
Fragments arranged by size:
1812 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 543 575 1903 1933 1935 1965 543
Size: 32 1328 30 2 30 2639
Fragments arranged by size:
2639 1328 32 30 30 2
HincII GTy'rAC
Cuts at: 696 1100 696
Size: 404 3657
HindIII A'AGCT_T
Cuts at: 401 401
Size: 4061
HpaI GTT'AAC
Cuts at: 696 696
Size: 4061
KasI G'GCGC_C
Cuts at: 1491 1739 1491
Size: 248 3813
KpnI G_GTAC'C
Cuts at: 1081 1081
Size: 4061
MluI A'CGCG_T
Cuts at: 3494 3494
Size: 4061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2732 2916 2732
Size: 184 3877
MscI TGG'CCA
Cuts at: 467 627 943 1822 467
Size: 160 316 879 2706
Fragments arranged by size:
2706 879 316 160
MslI CAynn'nnrTG
Cuts at: 848 2050 2177 848
Size: 1202 127 2732
Fragments arranged by size:
2732 1202 127
MspA1I CmG'CkG
Cuts at: 455 1172 1486 1846 2859 3104 455
Size: 717 314 360 1013 245 1412
Fragments arranged by size:
1412 1013 717 360 314 245
NaeI GCC'GGC
Cuts at: 2242 2242
Size: 4061
NarI GG'CG_CC
Cuts at: 1492 1740 1492
Size: 248 3813
NcoI C'CATG_G
Cuts at: 1011 1011
Size: 4061
NgoMIV G'CCGG_C
Cuts at: 2240 2240
Size: 4061
NspI r_CATG'y
Cuts at: 836 1114 1614 2521 836
Size: 278 500 907 2376
Fragments arranged by size:
2376 907 500 278
PasI CC'CwG_GG
Cuts at: 169 534 169
Size: 365 3696
PciI A'CATG_T
Cuts at: 832 2517 832
Size: 1685 2376
PmeI GTTT'AAAC
Cuts at: 1060 1060
Size: 4061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 4029
PstI C_TGCA'G
Cuts at: 1108 1793 1108
Size: 685 3376
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4061
PvuII CAG'CTG
Cuts at: 455 1486 1846 455
Size: 1031 360 2670
Fragments arranged by size:
2670 1031 360
RsrII CG'GwC_CG
Cuts at: 2256 2256
Size: 4061
SacI G_AGCT'C
Cuts at: 1075 1075
Size: 4061
SalI G'TCGA_C
Cuts at: 1098 1098
Size: 4061
SapI GCTCTTCn'nnn_
Cuts at: 2084 2294 2084
Size: 210 3851
SbfI CC_TGCA'GG
Cuts at: 1108 1108
Size: 4061
ScaI AGT'ACT
Cuts at: 3578 3578
Size: 4061
SfcI C'TryA_G
Cuts at: 33 353 496 890 987 1104 1789 2782 2973
Size: 320 143 394 97 117 685 993 191
Cuts at: 2973 33
Size: 1121
Fragments arranged by size:
1121 993 685 394 320 191 143 117
97
SfoI GGC'GCC
Cuts at: 1493 1741 1493
Size: 248 3813
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
SmaI CCC'GGG
Cuts at: 1083 1083
Size: 4061
SmlI C'TyrA_G
Cuts at: 1216 2131 2623 2885 1216
Size: 915 492 262 2392
Fragments arranged by size:
2392 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4061
SphI G_CATG'C
Cuts at: 1114 1614 1114
Size: 500 3561
StyI C'CwwG_G
Cuts at: 234 903 1001 1011 1195 1549 234
Size: 669 98 10 184 354 2746
Fragments arranged by size:
2746 669 354 184 98 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1644 1777 2236 2445 1644
Size: 133 459 209 3260
Fragments arranged by size:
3260 459 209 133
TatI w'GTAC_w
Cuts at: 3576 3576
Size: 4061
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 899 931 3578 3610 899
Size: 32 2647 32 1350
Fragments arranged by size:
2647 1350 32 32
Tth111I GACn'n_nGTC
Cuts at: 1858 1858
Size: 4061
XbaI T'CTAG_A
Cuts at: 1092 1092
Size: 4061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 315 1556 315
Size: 1241 2820
XmaI C'CCGG_G
Cuts at: 1081 1081
Size: 4061
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbsI BbvCI BcgI BfrBI BmtI Bpu10I
BsaXI BseRI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII EcoNI FseI FspAI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI