Restriction map for
pF1KE0799
(Circular) (Six-base) MAPSORT of: pF1KE0799.seq Check: 451 from: 1 to: 4616
pF1KE0799 4616 bp
With 182 enzymes: SgfI *
September 2, 2010 12:21 ..
AccI GT'mk_AC
Cuts at: 1654 1654
Size: 4616
Acc65I G'GTAC_C
Cuts at: 1632 1632
Size: 4616
AclI AA'CG_TT
Cuts at: 4456 4456
Size: 4616
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 2441 2873 3620 3888 916
Size: 1525 432 747 268 1644
Fragments arranged by size:
1644 1525 747 432 268
AflIII A'CryG_T
Cuts at: 1968 3072 4049 1968
Size: 1104 977 2535
Fragments arranged by size:
2535 1104 977
AgeI A'CCGG_T
Cuts at: 3004 3004
Size: 4616
AlwNI CAG_nnn'CTG
Cuts at: 1921 3488 1921
Size: 1567 3049
ApaLI G'TGCA_C
Cuts at: 3386 3386
Size: 4616
ApoI r'AATT_y
Cuts at: 217 555 615 973 1620 3969 4225 217
Size: 338 60 358 647 2349 256 608
Fragments arranged by size:
2349 647 608 358 338 256 60
AseI AT'TA_AT
Cuts at: 20 20
Size: 4616
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4616
AvaI C'yCGr_G
Cuts at: 1636 1636
Size: 4616
BamHI G'GATC_C
Cuts at: 328 1641 328
Size: 1313 3303
BanI G'GyrC_C
Cuts at: 1632 2046 2294 2329 1632
Size: 414 248 35 3919
Fragments arranged by size:
3919 414 248 35
BanII G_rGCy'C
Cuts at: 254 931 1630 2660 254
Size: 677 699 1030 2210
Fragments arranged by size:
2210 1030 699 677
BbeI G_GCGC'C
Cuts at: 2050 2298 2050
Size: 248 4368
BciVI GTATCCnnnnn_n'
Cuts at: 2514 3275 4616 2514
Size: 761 1341 2514
Fragments arranged by size:
2514 1341 761
BclI T'GATC_A
Cuts at: 866 1358 2136 866
Size: 492 778 3346
Fragments arranged by size:
3346 778 492
BfrBI ATG'CAT
Cuts at: 460 460
Size: 4616
BglII A'GATC_T
Cuts at: 173 2131 4168 173
Size: 1958 2037 621
Fragments arranged by size:
2037 1958 621
BlpI GC'TnA_GC
Cuts at: 1728 1728
Size: 4616
Bme1580I G_kGCm'C
Cuts at: 1494 1501 2241 2334 3390 1494
Size: 7 740 93 1056 2720
Fragments arranged by size:
2720 1056 740 93 7
BmgBI CAC'GTC
Cuts at: 1356 1356
Size: 4616
BmrI ACTGGGnnnn_n'
Cuts at: 1488 1549 1828 1909 2244 4388 1488
Size: 61 279 81 335 2144 1716
Fragments arranged by size:
2144 1716 335 279 81 61
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2983 2983
Size: 4616
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 776 931 776
Size: 155 4461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1115 1792 2671 3163 3461 1115
Size: 677 879 492 298 2270
Fragments arranged by size:
2270 879 677 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4320 4320
Size: 4616
BsaAI yAC'GTr
Cuts at: 720 1969 720
Size: 1249 3367
BsaBI GATnn'nnATC
Cuts at: 6 2130 4167 6
Size: 2124 2037 455
Fragments arranged by size:
2124 2037 455
BsaHI Gr'CG_yC
Cuts at: 2047 2295 2997 4493 2047
Size: 248 702 1496 2170
Fragments arranged by size:
2170 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1805 2326 3004 3278 3425 3836 1805
Size: 521 678 274 147 411 2585
Fragments arranged by size:
2585 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 259 262 692 1235 1318 1428 259
Size: 3 430 543 83 110 3447
Fragments arranged by size:
3447 543 430 110 83 3
BseYI C'CCAG_C
Cuts at: 2041 2799 3376 3872 2041
Size: 758 577 496 2785
Fragments arranged by size:
2785 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 527 638 1342 527
Size: 111 704 3801
Fragments arranged by size:
3801 704 111
BsiEI CG_ry'CG
Cuts at: 104 2158 2204 3412 104
Size: 2054 46 1208 1308
Fragments arranged by size:
2054 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 254 1630 2408 2598 3390 254
Size: 1376 778 190 792 1480
Fragments arranged by size:
1480 1376 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 254 931 1494 1501 1630 2241 2334 2408 2598
Size: 677 563 7 129 611 93 74 190
Cuts at: 2598 2660 3390 254
Size: 62 730 1480
Fragments arranged by size:
1480 730 677 611 563 190 129 93
74 62 7
BspEI T'CCGG_A
Cuts at: 3836 3836
Size: 4616
BspHI T'CATG_A
Cuts at: 1331 3792 1331
Size: 2461 2155
BspMI ACCTGCnnnn'nnnn_
Cuts at: 499 1666 1880 2182 2563 499
Size: 1167 214 302 381 2552
Fragments arranged by size:
2552 1167 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1083 2908 2962 4446 4611 50
Size: 1033 1825 54 1484 165 55
Fragments arranged by size:
1825 1484 1033 165 55 54
BsrDI GCAATG_nn'
Cuts at: 493 1292 2528 493
Size: 799 1236 2581
Fragments arranged by size:
2581 1236 799
BsrFI r'CCGG_y
Cuts at: 2614 2795 3004 2614
Size: 181 209 4226
Fragments arranged by size:
4226 209 181
BssSI C'ACGA_G
Cuts at: 2887 3245 2887
Size: 358 4258
BstAPI GCAn_nnn'nTGC
Cuts at: 1993 1993
Size: 4616
BstBI TT'CG_AA
Cuts at: 1068 2977 1068
Size: 1909 2707
BstEII G'GTnAC_C
Cuts at: 903 903
Size: 4616
BstYI r'GATC_y
Cuts at: 1 173 328 524 1641 2108 2131 2466 2712
Size: 172 155 196 1117 467 23 335 246
Cuts at: 2712 3724 3810 3822 4168 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1117 1012 467 449 346 335 246 196
172 155 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 117 117
Size: 4616
BtgI C'CryG_G
Cuts at: 334 984 1228 334
Size: 650 244 3722
Fragments arranged by size:
3722 650 244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2566 2747 2910 2566
Size: 181 163 4272
Fragments arranged by size:
4272 181 163
BtsI GCAGTG_nn'
Cuts at: 302 707 1859 302
Size: 405 1152 3059
Fragments arranged by size:
3059 1152 405
DraI TTT'AAA
Cuts at: 1341 1615 1341
Size: 274 4342
DrdI GACnn_nn'nnGTC
Cuts at: 136 426 2322 3180 136
Size: 290 1896 858 1572
Fragments arranged by size:
1896 1572 858 290
EaeI y'GGCC_r
Cuts at: 597 2201 2375 2766 2793 4138 597
Size: 1604 174 391 27 1345 1075
Fragments arranged by size:
1604 1345 1075 391 174 27
EagI C'GGCC_G
Cuts at: 2201 2201
Size: 4616
EarI CTCTTCn'nnn_
Cuts at: 892 1527 2639 2849 892
Size: 635 1112 210 2659
Fragments arranged by size:
2659 1112 635 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2824 3134 3280 4426 2824
Size: 310 146 1146 3014
Fragments arranged by size:
3014 1146 310 146
EcoICRI GAG'CTC
Cuts at: 252 1628 252
Size: 1376 3240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 776 916 931 2441 2873 3620 3888 776
Size: 140 15 1510 432 747 268 1504
Fragments arranged by size:
1510 1504 747 432 268 140 15
EcoNI CCTnn'n_nnAGG
Cuts at: 791 791
Size: 4616
EcoO109I rG'GnC_Cy
Cuts at: 649 1135 1755 649
Size: 486 620 3510
Fragments arranged by size:
3510 620 486
EcoRI G'AATT_C
Cuts at: 1620 1620
Size: 4616
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 332 2635 332
Size: 2303 2313
FspI TGC'GCA
Cuts at: 1291 2397 1291
Size: 1106 3510
FspAI rTGC'GCAy
Cuts at: 1291 1291
Size: 4616
HaeII r_GCGC'y
Cuts at: 2050 2298 3320 4299 2050
Size: 248 1022 979 2367
Fragments arranged by size:
2367 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2458 2488 2490 2520 2458
Size: 30 2 30 4554
Fragments arranged by size:
4554 30 30 2
HincII GTy'rAC
Cuts at: 288 1655 288
Size: 1367 3249
KasI G'GCGC_C
Cuts at: 2046 2294 2046
Size: 248 4368
KpnI G_GTAC'C
Cuts at: 1636 1636
Size: 4616
MfeI C'AATT_G
Cuts at: 545 545
Size: 4616
MluI A'CGCG_T
Cuts at: 4049 4049
Size: 4616
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3287 3471 3287
Size: 184 4432
MscI TGG'CCA
Cuts at: 599 2377 599
Size: 1778 2838
MslI CAynn'nnrTG
Cuts at: 2605 2732 2605
Size: 127 4489
MspA1I CmG'CkG
Cuts at: 1727 2041 2401 3414 3659 1727
Size: 314 360 1013 245 2684
Fragments arranged by size:
2684 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2797 2797
Size: 4616
NarI GG'CG_CC
Cuts at: 2047 2295 2047
Size: 248 4368
NcoI C'CATG_G
Cuts at: 984 984
Size: 4616
NgoMIV G'CCGG_C
Cuts at: 2795 2795
Size: 4616
NsiI A_TGCA'T
Cuts at: 462 462
Size: 4616
NspI r_CATG'y
Cuts at: 1669 2169 3076 1669
Size: 500 907 3209
Fragments arranged by size:
3209 907 500
PciI A'CATG_T
Cuts at: 3072 3072
Size: 4616
PflMI CCAn_nnn'nTGG
Cuts at: 550 550
Size: 4616
PfoI T'CCnGG_A
Cuts at: 753 1027 753
Size: 274 4342
PmeI GTTT'AAAC
Cuts at: 1615 1615
Size: 4616
PmlI CAC'GTG
Cuts at: 720 720
Size: 4616
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 3755
Size: 32 4584
PstI C_TGCA'G
Cuts at: 1451 1663 2348 1451
Size: 212 685 3719
Fragments arranged by size:
3719 685 212
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4616
PvuII CAG'CTG
Cuts at: 2041 2401 2041
Size: 360 4256
RsrII CG'GwC_CG
Cuts at: 2811 2811
Size: 4616
SacI G_AGCT'C
Cuts at: 254 1630 254
Size: 1376 3240
SalI G'TCGA_C
Cuts at: 1653 1653
Size: 4616
SapI GCTCTTCn'nnn_
Cuts at: 2639 2849 2639
Size: 210 4406
SbfI CC_TGCA'GG
Cuts at: 1663 1663
Size: 4616
ScaI AGT'ACT
Cuts at: 4133 4133
Size: 4616
SfcI C'TryA_G
Cuts at: 33 788 1447 1659 2344 3337 3528 33
Size: 755 659 212 685 993 191 1121
Fragments arranged by size:
1121 993 755 685 659 212 191
SfoI GGC'GCC
Cuts at: 2048 2296 2048
Size: 248 4368
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4616
SmaI CCC'GGG
Cuts at: 1638 1638
Size: 4616
SmlI C'TyrA_G
Cuts at: 1130 1771 2686 3178 3440 1130
Size: 641 915 492 262 2306
Fragments arranged by size:
2306 915 641 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4616
SphI G_CATG'C
Cuts at: 1669 2169 1669
Size: 500 4116
SspI AAT'ATT
Cuts at: 768 768
Size: 4616
StyI C'CwwG_G
Cuts at: 610 984 1101 1750 2104 610
Size: 374 117 649 354 3122
Fragments arranged by size:
3122 649 374 354 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1455 2199 2332 2791 3000 1455
Size: 744 133 459 209 3071
Fragments arranged by size:
3071 744 459 209 133
TatI w'GTAC_w
Cuts at: 4131 4131
Size: 4616
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 659 691 4133 4165 659
Size: 32 3442 32 1110
Fragments arranged by size:
3442 1110 32 32
Tth111I GACn'n_nGTC
Cuts at: 2413 2413
Size: 4616
XbaI T'CTAG_A
Cuts at: 1647 1647
Size: 4616
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1035 1501 2111 1035
Size: 466 610 3540
Fragments arranged by size:
3540 610 466
XmaI C'CCGG_G
Cuts at: 1636 1636
Size: 4616
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PmlI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI HindIII
HpaI NdeI NheI NotI NruI PacI PasI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI