Restriction map for
pF1KE0796
(Circular) (Six-base) MAPSORT of: pF1KE0796.seq Check: 1331 from: 1 to: 4385
pF1KE0796 4385 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AccI GT'mk_AC
Cuts at: 1423 1423
Size: 4385
Acc65I G'GTAC_C
Cuts at: 1401 1401
Size: 4385
AclI AA'CG_TT
Cuts at: 4225 4225
Size: 4385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2210 2642 3389 3657 2210
Size: 432 747 268 2938
Fragments arranged by size:
2938 747 432 268
AfeI AGC'GCT
Cuts at: 826 826
Size: 4385
AflIII A'CryG_T
Cuts at: 1737 2841 3818 1737
Size: 1104 977 2304
Fragments arranged by size:
2304 1104 977
AgeI A'CCGG_T
Cuts at: 2773 2773
Size: 4385
AlwNI CAG_nnn'CTG
Cuts at: 1690 3257 1690
Size: 1567 2818
ApaI G_GGCC'C
Cuts at: 1332 1332
Size: 4385
ApaLI G'TGCA_C
Cuts at: 3155 3155
Size: 4385
ApoI r'AATT_y
Cuts at: 1389 3738 3994 1389
Size: 2349 256 1780
Fragments arranged by size:
2349 1780 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4385
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
AvaI C'yCGr_G
Cuts at: 1405 1405
Size: 4385
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 702 735 702
Size: 33 4352
BamHI G'GATC_C
Cuts at: 1410 1410
Size: 4385
BanI G'GyrC_C
Cuts at: 301 1401 1815 2063 2098 301
Size: 1100 414 248 35 2588
Fragments arranged by size:
2588 1100 414 248 35
BanII G_rGCy'C
Cuts at: 1332 1366 1399 2429 1332
Size: 34 33 1030 3288
Fragments arranged by size:
3288 1030 34 33
BbeI G_GCGC'C
Cuts at: 1819 2067 1819
Size: 248 4137
BbsI GAAGACnn'nnnn_
Cuts at: 786 1361 786
Size: 575 3810
BbvCI CC'TCA_GC
Cuts at: 1193 1193
Size: 4385
BciVI GTATCCnnnnn_n'
Cuts at: 2283 3044 4385 2283
Size: 761 1341 2283
Fragments arranged by size:
2283 1341 761
BclI T'GATC_A
Cuts at: 1905 1905
Size: 4385
BglII A'GATC_T
Cuts at: 1900 3937 1900
Size: 2037 2348
BlpI GC'TnA_GC
Cuts at: 1497 1497
Size: 4385
Bme1580I G_kGCm'C
Cuts at: 365 1008 1332 2010 2103 3159 365
Size: 643 324 678 93 1056 1591
Fragments arranged by size:
1591 1056 678 643 324 93
BmrI ACTGGGnnnn_n'
Cuts at: 1011 1597 1678 2013 4157 1011
Size: 586 81 335 2144 1239
Fragments arranged by size:
2144 1239 586 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 4385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 632 647 999 1103 1135 632
Size: 15 352 104 32 3882
Fragments arranged by size:
3882 352 104 32 15
Bpu10I CC'TnA_GC
Cuts at: 1193 1193
Size: 4385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1561 2440 2932 3230 1561
Size: 879 492 298 2716
Fragments arranged by size:
2716 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4089 4089
Size: 4385
BsaAI yAC'GTr
Cuts at: 1738 1738
Size: 4385
BsaBI GATnn'nnATC
Cuts at: 6 1899 3936 6
Size: 1893 2037 455
Fragments arranged by size:
2037 1893 455
BsaHI Gr'CG_yC
Cuts at: 1816 2064 2766 4262 1816
Size: 248 702 1496 1939
Fragments arranged by size:
1939 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1574 2095 2773 3047 3194 3605 1574
Size: 521 678 274 147 411 2354
Fragments arranged by size:
2354 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 385 415 1021 1051 385
Size: 30 606 30 3719
Fragments arranged by size:
3719 606 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 201 1264 201
Size: 1063 3322
BseYI C'CCAG_C
Cuts at: 914 1810 2568 3145 3641 914
Size: 896 758 577 496 1658
Fragments arranged by size:
1658 896 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1126 1126
Size: 4385
BsiEI CG_ry'CG
Cuts at: 104 1927 1973 3181 104
Size: 1823 46 1208 1308
Fragments arranged by size:
1823 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1399 2177 2367 3159 1399
Size: 778 190 792 2625
Fragments arranged by size:
2625 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 388 1317 388
Size: 929 3456
Bsp1286I G_dGCh'C
Cuts at: 365 1008 1332 1366 1399 2010 2103 2177 2367
Size: 643 324 34 33 611 93 74 190
Cuts at: 2367 2429 3159 365
Size: 62 730 1591
Fragments arranged by size:
1591 730 643 611 324 190 93 74
62 34 33
BspEI T'CCGG_A
Cuts at: 3605 3605
Size: 4385
BspHI T'CATG_A
Cuts at: 3561 3561
Size: 4385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1435 1649 1951 2332 1435
Size: 214 302 381 3488
Fragments arranged by size:
3488 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2677 2731 4215 4380 50
Size: 2627 54 1484 165 55
Fragments arranged by size:
2627 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 602 2297 602
Size: 1695 2690
BsrFI r'CCGG_y
Cuts at: 2383 2564 2773 2383
Size: 181 209 3995
Fragments arranged by size:
3995 209 181
BsrGI T'GTAC_A
Cuts at: 248 248
Size: 4385
BssSI C'ACGA_G
Cuts at: 2656 3014 2656
Size: 358 4027
BstAPI GCAn_nnn'nTGC
Cuts at: 941 1762 941
Size: 821 3564
BstBI TT'CG_AA
Cuts at: 2746 2746
Size: 4385
BstXI CCAn_nnnn'nTGG
Cuts at: 555 1047 555
Size: 492 3893
BstYI r'GATC_y
Cuts at: 1 1410 1877 1900 2235 2481 3493 3579 3591
Size: 1409 467 23 335 246 1012 86 12
Cuts at: 3591 3937 1
Size: 346 449
Fragments arranged by size:
1409 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 779 779
Size: 4385
BtgI C'CryG_G
Cuts at: 107 1099 1347 107
Size: 992 248 3145
Fragments arranged by size:
3145 992 248
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 153 1185 2335 2516 2679 153
Size: 1032 1150 181 163 1859
Fragments arranged by size:
1859 1150 1032 181 163
BtsI GCAGTG_nn'
Cuts at: 699 894 1628 699
Size: 195 734 3456
Fragments arranged by size:
3456 734 195
DraI TTT'AAA
Cuts at: 1384 1384
Size: 4385
DrdI GACnn_nn'nnGTC
Cuts at: 2091 2949 2091
Size: 858 3527
EaeI y'GGCC_r
Cuts at: 1970 2144 2535 2562 3907 1970
Size: 174 391 27 1345 2448
Fragments arranged by size:
2448 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1970 1970
Size: 4385
EarI CTCTTCn'nnn_
Cuts at: 1189 1226 2408 2618 1189
Size: 37 1182 210 2956
Fragments arranged by size:
2956 1182 210 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2593 2903 3049 4195 2593
Size: 310 146 1146 2783
Fragments arranged by size:
2783 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1397 1397
Size: 4385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 632 647 999 1103 1135 2210 2642 3389 3657
Size: 15 352 104 32 1075 432 747 268
Cuts at: 3657 632
Size: 1360
Fragments arranged by size:
1360 1075 747 432 352 268 104 32
15
EcoNI CCTnn'n_nnAGG
Cuts at: 1336 1336
Size: 4385
EcoO109I rG'GnC_Cy
Cuts at: 1329 1524 1329
Size: 195 4190
EcoRI G'AATT_C
Cuts at: 1389 1389
Size: 4385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 4385
FspI TGC'GCA
Cuts at: 2166 2166
Size: 4385
HaeII r_GCGC'y
Cuts at: 828 1079 1819 2067 3089 4068 828
Size: 251 740 248 1022 979 1145
Fragments arranged by size:
1145 1022 979 740 251 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 387 419 485 517 1021 1053 2227 2257 2259
Size: 32 66 32 504 32 1174 30 2
Cuts at: 2259 2289 387
Size: 30 2483
Fragments arranged by size:
2483 1174 504 66 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1424 1424
Size: 4385
HindIII A'AGCT_T
Cuts at: 1288 1288
Size: 4385
KasI G'GCGC_C
Cuts at: 1815 2063 1815
Size: 248 4137
KpnI G_GTAC'C
Cuts at: 1405 1405
Size: 4385
MluI A'CGCG_T
Cuts at: 3818 3818
Size: 4385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 107 349 3056 3240 107
Size: 242 2707 184 1252
Fragments arranged by size:
2707 1252 242 184
MscI TGG'CCA
Cuts at: 2146 2146
Size: 4385
MslI CAynn'nnrTG
Cuts at: 533 2374 2501 533
Size: 1841 127 2417
Fragments arranged by size:
2417 1841 127
MspA1I CmG'CkG
Cuts at: 1192 1496 1810 2170 3183 3428 1192
Size: 304 314 360 1013 245 2149
Fragments arranged by size:
2149 1013 360 314 304 245
NaeI GCC'GGC
Cuts at: 2566 2566
Size: 4385
NarI GG'CG_CC
Cuts at: 1816 2064 1816
Size: 248 4137
NcoI C'CATG_G
Cuts at: 107 1099 107
Size: 992 3393
NgoMIV G'CCGG_C
Cuts at: 2564 2564
Size: 4385
NspI r_CATG'y
Cuts at: 1438 1938 2845 1438
Size: 500 907 2978
Fragments arranged by size:
2978 907 500
PciI A'CATG_T
Cuts at: 2841 2841
Size: 4385
PfoI T'CCnGG_A
Cuts at: 968 968
Size: 4385
PmeI GTTT'AAAC
Cuts at: 1384 1384
Size: 4385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 4353
PspOMI G'GGCC_C
Cuts at: 1328 1328
Size: 4385
PstI C_TGCA'G
Cuts at: 765 937 1094 1432 2117 765
Size: 172 157 338 685 3033
Fragments arranged by size:
3033 685 338 172 157
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4385
PvuII CAG'CTG
Cuts at: 1192 1810 2170 1192
Size: 618 360 3407
Fragments arranged by size:
3407 618 360
RsrII CG'GwC_CG
Cuts at: 2580 2580
Size: 4385
SacI G_AGCT'C
Cuts at: 1399 1399
Size: 4385
SalI G'TCGA_C
Cuts at: 1422 1422
Size: 4385
SapI GCTCTTCn'nnn_
Cuts at: 1189 2408 2618 1189
Size: 1219 210 2956
Fragments arranged by size:
2956 1219 210
SbfI CC_TGCA'GG
Cuts at: 1432 1432
Size: 4385
ScaI AGT'ACT
Cuts at: 3902 3902
Size: 4385
SfcI C'TryA_G
Cuts at: 33 137 313 761 933 1090 1428 2113 3106
Size: 104 176 448 172 157 338 685 993
Cuts at: 3106 3297 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 448 338 191 176 172
157 104
SfoI GGC'GCC
Cuts at: 1817 2065 1817
Size: 248 4137
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
SmaI CCC'GGG
Cuts at: 1407 1407
Size: 4385
SmlI C'TyrA_G
Cuts at: 1540 2455 2947 3209 1540
Size: 915 492 262 2716
Fragments arranged by size:
2716 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4385
SphI G_CATG'C
Cuts at: 1438 1938 1438
Size: 500 3885
StyI C'CwwG_G
Cuts at: 107 1099 1519 1873 107
Size: 992 420 354 2619
Fragments arranged by size:
2619 992 420 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1092 1112 1968 2101 2560 2769 1092
Size: 20 856 133 459 209 2708
Fragments arranged by size:
2708 856 459 209 133 20
TatI w'GTAC_w
Cuts at: 248 3900 248
Size: 3652 733
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3902 3934 3902
Size: 32 4353
Tth111I GACn'n_nGTC
Cuts at: 2182 2182
Size: 4385
XbaI T'CTAG_A
Cuts at: 1416 1416
Size: 4385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1345 1880 1345
Size: 535 3850
XmaI C'CCGG_G
Cuts at: 1405 1405
Size: 4385
XmnI GAAnn'nnTTC
Cuts at: 161 161
Size: 4385
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BcgI BfrBI BglI BmgBI BmtI BsiWI BsmI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI