Restriction map for
pF1KE0793
(Circular) (Six-base) MAPSORT of: pF1KE0793.seq Check: 7395 from: 1 to: 4049
pF1KE0793 4049 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 707 977 1087 707
Size: 270 110 3669
Fragments arranged by size:
3669 270 110
Acc65I G'GTAC_C
Cuts at: 1065 1065
Size: 4049
AclI AA'CG_TT
Cuts at: 3889 3889
Size: 4049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1874 2306 3053 3321 1874
Size: 432 747 268 2602
Fragments arranged by size:
2602 747 432 268
AflIII A'CryG_T
Cuts at: 411 1401 2505 3482 411
Size: 990 1104 977 978
Fragments arranged by size:
1104 990 978 977
AgeI A'CCGG_T
Cuts at: 2437 2437
Size: 4049
AlwNI CAG_nnn'CTG
Cuts at: 559 1354 2921 559
Size: 795 1567 1687
Fragments arranged by size:
1687 1567 795
ApaI G_GGCC'C
Cuts at: 1016 1016
Size: 4049
ApaLI G'TGCA_C
Cuts at: 2819 2819
Size: 4049
ApoI r'AATT_y
Cuts at: 479 718 1053 3402 3658 479
Size: 239 335 2349 256 870
Fragments arranged by size:
2349 870 335 256 239
AseI AT'TA_AT
Cuts at: 20 20
Size: 4049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
AvaI C'yCGr_G
Cuts at: 271 569 632 1069 271
Size: 298 63 437 3251
Fragments arranged by size:
3251 437 298 63
BamHI G'GATC_C
Cuts at: 1074 1074
Size: 4049
BanI G'GyrC_C
Cuts at: 1065 1479 1727 1762 1065
Size: 414 248 35 3352
Fragments arranged by size:
3352 414 248 35
BanII G_rGCy'C
Cuts at: 517 570 1016 1063 2093 517
Size: 53 446 47 1030 2473
Fragments arranged by size:
2473 1030 446 53 47
BbeI G_GCGC'C
Cuts at: 1483 1731 1483
Size: 248 3801
BbsI GAAGACnn'nnnn_
Cuts at: 658 658
Size: 4049
BciVI GTATCCnnnnn_n'
Cuts at: 1947 2708 4049 1947
Size: 761 1341 1947
Fragments arranged by size:
1947 1341 761
BclI T'GATC_A
Cuts at: 1569 1569
Size: 4049
BglI GCCn_nnn'nGGC
Cuts at: 405 405
Size: 4049
BglII A'GATC_T
Cuts at: 1564 3601 1564
Size: 2037 2012
BlpI GC'TnA_GC
Cuts at: 929 1161 929
Size: 232 3817
Bme1580I G_kGCm'C
Cuts at: 1016 1674 1767 2823 1016
Size: 658 93 1056 2242
Fragments arranged by size:
2242 1056 658 93
BmgBI CAC'GTC
Cuts at: 414 489 414
Size: 75 3974
BmrI ACTGGGnnnn_n'
Cuts at: 126 1261 1342 1677 3821 126
Size: 1135 81 335 2144 354
Fragments arranged by size:
2144 1135 354 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 531 2416 531
Size: 1885 2164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1225 2104 2596 2894 1225
Size: 879 492 298 2380
Fragments arranged by size:
2380 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3753 3753
Size: 4049
BsaAI yAC'GTr
Cuts at: 1402 1402
Size: 4049
BsaBI GATnn'nnATC
Cuts at: 6 1563 3600 6
Size: 1557 2037 455
Fragments arranged by size:
2037 1557 455
BsaHI Gr'CG_yC
Cuts at: 1480 1728 2430 3926 1480
Size: 248 702 1496 1603
Fragments arranged by size:
1603 1496 702 248
BsaWI w'CCGG_w
Cuts at: 431 1238 1759 2437 2711 2858 3269 431
Size: 807 521 678 274 147 411 1211
Fragments arranged by size:
1211 807 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 851 881 851
Size: 30 4019
BseYI C'CCAG_C
Cuts at: 113 1474 2232 2809 3305 113
Size: 1361 758 577 496 857
Fragments arranged by size:
1361 857 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 593 866 1591 1637 2845 104
Size: 489 273 725 46 1208 1308
Fragments arranged by size:
1308 1208 725 489 273 46
BsiHKAI G_wGCw'C
Cuts at: 1063 1841 2031 2823 1063
Size: 778 190 792 2289
Fragments arranged by size:
2289 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 420 420
Size: 4049
Bsp1286I G_dGCh'C
Cuts at: 517 570 1016 1063 1674 1767 1841 2031 2093
Size: 53 446 47 611 93 74 190 62
Cuts at: 2093 2823 517
Size: 730 1743
Fragments arranged by size:
1743 730 611 446 190 93 74 62
53 47
BspEI T'CCGG_A
Cuts at: 3269 3269
Size: 4049
BspHI T'CATG_A
Cuts at: 3225 3225
Size: 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1099 1313 1615 1996 1099
Size: 214 302 381 3152
Fragments arranged by size:
3152 381 302 214
BsrBI CCG'CTC
Cuts at: 50 366 510 862 2341 2395 3879 4044 50
Size: 316 144 352 1479 54 1484 165 55
Fragments arranged by size:
1484 1479 352 316 165 144 55 54
BsrDI GCAATG_nn'
Cuts at: 1961 1961
Size: 4049
BsrFI r'CCGG_y
Cuts at: 754 2047 2228 2437 754
Size: 1293 181 209 2366
Fragments arranged by size:
2366 1293 209 181
BsrGI T'GTAC_A
Cuts at: 248 248
Size: 4049
BssSI C'ACGA_G
Cuts at: 2320 2678 2320
Size: 358 3691
BstAPI GCAn_nnn'nTGC
Cuts at: 1426 1426
Size: 4049
BstBI TT'CG_AA
Cuts at: 2410 2410
Size: 4049
BstYI r'GATC_y
Cuts at: 1 1074 1541 1564 1899 2145 3157 3243 3255
Size: 1073 467 23 335 246 1012 86 12
Cuts at: 3255 3601 1
Size: 346 449
Fragments arranged by size:
1073 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 626 1016 1040 107
Size: 519 390 24 3116
Fragments arranged by size:
3116 519 390 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 291 1999 2180 2343 291
Size: 1708 181 163 1997
Fragments arranged by size:
1997 1708 181 163
BtsI GCAGTG_nn'
Cuts at: 734 1292 734
Size: 558 3491
DraI TTT'AAA
Cuts at: 1048 1048
Size: 4049
DrdI GACnn_nn'nnGTC
Cuts at: 1755 2613 1755
Size: 858 3191
EaeI y'GGCC_r
Cuts at: 210 1634 1808 2199 2226 3571 210
Size: 1424 174 391 27 1345 688
Fragments arranged by size:
1424 1345 688 391 174 27
EagI C'GGCC_G
Cuts at: 1634 1634
Size: 4049
EarI CTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 134 2257 2567 2713 3859 134
Size: 2123 310 146 1146 324
Fragments arranged by size:
2123 1146 324 310 146
EcoICRI GAG'CTC
Cuts at: 1061 1061
Size: 4049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1874 2306 3053 3321 1874
Size: 432 747 268 2602
Fragments arranged by size:
2602 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 314 441 1188 314
Size: 127 747 3175
Fragments arranged by size:
3175 747 127
EcoRI G'AATT_C
Cuts at: 718 1053 718
Size: 335 3714
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 4049
FspI TGC'GCA
Cuts at: 1830 1830
Size: 4049
HaeII r_GCGC'y
Cuts at: 1483 1731 2753 3732 1483
Size: 248 1022 979 1800
Fragments arranged by size:
1800 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1891 1921 1923 1953 1891
Size: 30 2 30 3987
Fragments arranged by size:
3987 30 30 2
HincII GTy'rAC
Cuts at: 1088 1088
Size: 4049
KasI G'GCGC_C
Cuts at: 1479 1727 1479
Size: 248 3801
KpnI G_GTAC'C
Cuts at: 1069 1069
Size: 4049
MluI A'CGCG_T
Cuts at: 3482 3482
Size: 4049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 401 443 2720 2904 401
Size: 42 2277 184 1546
Fragments arranged by size:
2277 1546 184 42
MscI TGG'CCA
Cuts at: 1810 1810
Size: 4049
MslI CAynn'nnrTG
Cuts at: 2038 2165 2038
Size: 127 3922
MspA1I CmG'CkG
Cuts at: 937 943 985 1018 1160 1474 1834 2847 3092
Size: 6 42 33 142 314 360 1013 245
Cuts at: 3092 937
Size: 1894
Fragments arranged by size:
1894 1013 360 314 245 142 42 33
6
NaeI GCC'GGC
Cuts at: 2230 2230
Size: 4049
NarI GG'CG_CC
Cuts at: 1480 1728 1480
Size: 248 3801
NcoI C'CATG_G
Cuts at: 107 1040 107
Size: 933 3116
NgoMIV G'CCGG_C
Cuts at: 2228 2228
Size: 4049
NspI r_CATG'y
Cuts at: 1102 1602 2509 1102
Size: 500 907 2642
Fragments arranged by size:
2642 907 500
PciI A'CATG_T
Cuts at: 2505 2505
Size: 4049
PflMI CCAn_nnn'nTGG
Cuts at: 677 677
Size: 4049
PmeI GTTT'AAAC
Cuts at: 1048 1048
Size: 4049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4017
PpuMI rG'GwC_Cy
Cuts at: 314 441 314
Size: 127 3922
PshAI GACnn'nnGTC
Cuts at: 220 220
Size: 4049
PspOMI G'GGCC_C
Cuts at: 1012 1012
Size: 4049
PstI C_TGCA'G
Cuts at: 942 1096 1781 942
Size: 154 685 3210
Fragments arranged by size:
3210 685 154
PvuI CG_AT'CG
Cuts at: 104 593 104
Size: 489 3560
PvuII CAG'CTG
Cuts at: 985 1474 1834 985
Size: 489 360 3200
Fragments arranged by size:
3200 489 360
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 4049
SacI G_AGCT'C
Cuts at: 1063 1063
Size: 4049
SacII CC_GC'GG
Cuts at: 1019 1019
Size: 4049
SalI G'TCGA_C
Cuts at: 1086 1086
Size: 4049
SanDI GG'GwC_CC
Cuts at: 314 441 314
Size: 127 3922
SapI GCTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
SbfI CC_TGCA'GG
Cuts at: 1096 1096
Size: 4049
ScaI AGT'ACT
Cuts at: 3566 3566
Size: 4049
SfcI C'TryA_G
Cuts at: 33 383 918 938 954 1026 1092 1777 2770
Size: 350 535 20 16 72 66 685 993
Cuts at: 2770 2961 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 535 350 191 72 66
20 16
SfoI GGC'GCC
Cuts at: 1481 1729 1481
Size: 248 3801
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
SmaI CCC'GGG
Cuts at: 571 1071 571
Size: 500 3549
SmlI C'TyrA_G
Cuts at: 1204 2119 2611 2873 1204
Size: 915 492 262 2380
Fragments arranged by size:
2380 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4049
SphI G_CATG'C
Cuts at: 1102 1602 1102
Size: 500 3549
StyI C'CwwG_G
Cuts at: 107 518 1040 1183 1537 107
Size: 411 522 143 354 2619
Fragments arranged by size:
2619 522 411 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 578 1632 1765 2224 2433 578
Size: 1054 133 459 209 2194
Fragments arranged by size:
2194 1054 459 209 133
TatI w'GTAC_w
Cuts at: 248 3564 248
Size: 3316 733
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 830 851 862 883 3566 3598 830
Size: 21 11 21 2683 32 1281
Fragments arranged by size:
2683 1281 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 1846 1846
Size: 4049
XbaI T'CTAG_A
Cuts at: 1080 1080
Size: 4049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1544 1544
Size: 4049
XmaI C'CCGG_G
Cuts at: 569 1069 569
Size: 500 3549
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI BpmI
Bpu10I BseRI BsgI BsiWI BsmI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PsiI PspXI PsrI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI