Restriction map for
pF1KE0788
(Circular) (Six-base) MAPSORT of: pF1KE0788.seq Check: 4279 from: 1 to: 4370
pF1KE0788 4370 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 505 718 1358 505
Size: 213 640 3517
Fragments arranged by size:
3517 640 213
AatII G_ACGT'C
Cuts at: 683 839 683
Size: 156 4214
AccI GT'mk_AC
Cuts at: 1408 1408
Size: 4370
Acc65I G'GTAC_C
Cuts at: 1386 1386
Size: 4370
AclI AA'CG_TT
Cuts at: 4210 4210
Size: 4370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 640 826 1344 2195 2627 3374 3642 178
Size: 462 186 518 851 432 747 268 906
Fragments arranged by size:
906 851 747 518 462 432 268 186
AfeI AGC'GCT
Cuts at: 442 1252 442
Size: 810 3560
AflIII A'CryG_T
Cuts at: 1722 2826 3803 1722
Size: 1104 977 2289
Fragments arranged by size:
2289 1104 977
AgeI A'CCGG_T
Cuts at: 2758 2758
Size: 4370
AhdI GACnn_n'nnGTC
Cuts at: 1167 1278 1167
Size: 111 4259
AlwNI CAG_nnn'CTG
Cuts at: 135 393 1675 3242 135
Size: 258 1282 1567 1263
Fragments arranged by size:
1567 1282 1263 258
ApaI G_GGCC'C
Cuts at: 745 745
Size: 4370
ApaLI G'TGCA_C
Cuts at: 3140 3140
Size: 4370
ApoI r'AATT_y
Cuts at: 1374 3723 3979 1374
Size: 2349 256 1765
Fragments arranged by size:
2349 1765 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4370
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
AvaI C'yCGr_G
Cuts at: 751 964 1155 1390 751
Size: 213 191 235 3731
Fragments arranged by size:
3731 235 213 191
BamHI G'GATC_C
Cuts at: 1395 1395
Size: 4370
BanI G'GyrC_C
Cuts at: 493 706 960 1386 1800 2048 2083 493
Size: 213 254 426 414 248 35 2780
Fragments arranged by size:
2780 426 414 254 248 213 35
BanII G_rGCy'C
Cuts at: 289 338 701 745 1127 1384 2414 289
Size: 49 363 44 382 257 1030 2245
Fragments arranged by size:
2245 1030 382 363 257 49 44
BbeI G_GCGC'C
Cuts at: 1804 2052 1804
Size: 248 4122
BbsI GAAGACnn'nnnn_
Cuts at: 535 675 1312 535
Size: 140 637 3593
Fragments arranged by size:
3593 637 140
BciVI GTATCCnnnnn_n'
Cuts at: 2268 3029 4370 2268
Size: 761 1341 2268
Fragments arranged by size:
2268 1341 761
BclI T'GATC_A
Cuts at: 1890 1890
Size: 4370
BglI GCCn_nnn'nGGC
Cuts at: 112 958 112
Size: 846 3524
BglII A'GATC_T
Cuts at: 1885 3922 1885
Size: 2037 2333
BlpI GC'TnA_GC
Cuts at: 1482 1482
Size: 4370
Bme1580I G_kGCm'C
Cuts at: 709 745 1995 2088 3144 709
Size: 36 1250 93 1056 1935
Fragments arranged by size:
1935 1250 1056 93 36
BmgBI CAC'GTC
Cuts at: 540 1275 540
Size: 735 3635
BmrI ACTGGGnnnn_n'
Cuts at: 1582 1663 1998 4142 1582
Size: 81 335 2144 1810
Fragments arranged by size:
2144 1810 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 4370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 1285 487
Size: 798 3572
Bpu10I CC'TnA_GC
Cuts at: 1336 1336
Size: 4370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 1546 2425 2917 3215 709
Size: 837 879 492 298 1864
Fragments arranged by size:
1864 879 837 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1203 4074 1203
Size: 2871 1499
BsaAI yAC'GTr
Cuts at: 1723 1723
Size: 4370
BsaBI GATnn'nnATC
Cuts at: 6 1884 3921 6
Size: 1878 2037 455
Fragments arranged by size:
2037 1878 455
BsaHI Gr'CG_yC
Cuts at: 115 680 836 1279 1331 1801 2049 2751 4247
Size: 565 156 443 52 470 248 702 1496
Cuts at: 4247 115
Size: 238
Fragments arranged by size:
1496 702 565 470 443 248 238 156
52
BsaWI w'CCGG_w
Cuts at: 524 1559 2080 2758 3032 3179 3590 524
Size: 1035 521 678 274 147 411 1304
Fragments arranged by size:
1304 1035 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 595 595
Size: 4370
BseYI C'CCAG_C
Cuts at: 215 330 1795 2553 3130 3626 215
Size: 115 1465 758 577 496 959
Fragments arranged by size:
1465 959 758 577 496 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 667
Size: 4370
BsiEI CG_ry'CG
Cuts at: 104 906 1912 1958 3166 104
Size: 802 1006 46 1208 1308
Fragments arranged by size:
1308 1208 1006 802 46
BsiHKAI G_wGCw'C
Cuts at: 289 701 1384 2162 2352 3144 289
Size: 412 683 778 190 792 1515
Fragments arranged by size:
1515 792 778 683 412 190
BsmI GAATG_Cn'
Cuts at: 758 758
Size: 4370
BsmBI CGTCTCn'nnnn_
Cuts at: 1252 1252
Size: 4370
Bsp1286I G_dGCh'C
Cuts at: 289 338 701 709 745 1127 1384 1995 2088
Size: 49 363 8 36 382 257 611 93
Cuts at: 2088 2162 2352 2414 3144 289
Size: 74 190 62 730 1515
Fragments arranged by size:
1515 730 611 382 363 257 190 93
74 62 49 36 8
BspEI T'CCGG_A
Cuts at: 524 3590 524
Size: 3066 1304
BspHI T'CATG_A
Cuts at: 404 3546 404
Size: 3142 1228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 505 718 1358 1420 1634 1936 2317 505
Size: 213 640 62 214 302 381 2558
Fragments arranged by size:
2558 640 381 302 214 213 62
BsrBI CCG'CTC
Cuts at: 50 269 867 2662 2716 4200 4365 50
Size: 219 598 1795 54 1484 165 55
Fragments arranged by size:
1795 1484 598 219 165 55 54
BsrDI GCAATG_nn'
Cuts at: 542 2282 542
Size: 1740 2630
BsrFI r'CCGG_y
Cuts at: 532 940 2368 2549 2758 532
Size: 408 1428 181 209 2144
Fragments arranged by size:
2144 1428 408 209 181
BssSI C'ACGA_G
Cuts at: 2641 2999 2641
Size: 358 4012
BstAPI GCAn_nnn'nTGC
Cuts at: 1747 1747
Size: 4370
BstBI TT'CG_AA
Cuts at: 2731 2731
Size: 4370
BstYI r'GATC_y
Cuts at: 1 1395 1862 1885 2220 2466 3478 3564 3576
Size: 1394 467 23 335 246 1012 86 12
Cuts at: 3576 3922 1
Size: 346 449
Fragments arranged by size:
1394 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 692 1318 107
Size: 585 626 3159
Fragments arranged by size:
3159 626 585
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 672 2320 2501 2664 672
Size: 1648 181 163 2378
Fragments arranged by size:
2378 1648 181 163
BtsI GCAGTG_nn'
Cuts at: 655 1613 655
Size: 958 3412
DraI TTT'AAA
Cuts at: 1369 1369
Size: 4370
DrdI GACnn_nn'nnGTC
Cuts at: 1096 2076 2934 1096
Size: 980 858 2532
Fragments arranged by size:
2532 980 858
EaeI y'GGCC_r
Cuts at: 248 501 534 903 1955 2129 2520 2547 3892
Size: 253 33 369 1052 174 391 27 1345
Cuts at: 3892 248
Size: 726
Fragments arranged by size:
1345 1052 726 391 369 253 174 33
27
EagI C'GGCC_G
Cuts at: 903 1955 903
Size: 1052 3318
EarI CTCTTCn'nnn_
Cuts at: 616 1115 1201 2393 2603 616
Size: 499 86 1192 210 2383
Fragments arranged by size:
2383 1192 499 210 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 198 2578 2888 3034 4180 198
Size: 2380 310 146 1146 388
Fragments arranged by size:
2380 1146 388 310 146
EcoICRI GAG'CTC
Cuts at: 287 699 1382 287
Size: 412 683 3275
Fragments arranged by size:
3275 683 412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 487 640 826 1285 1344 2195 2627 3374
Size: 309 153 186 459 59 851 432 747
Cuts at: 3374 3642 178
Size: 268 906
Fragments arranged by size:
906 851 747 459 432 309 268 186
153 59
EcoNI CCTnn'n_nnAGG
Cuts at: 1359 1359
Size: 4370
EcoO109I rG'GnC_Cy
Cuts at: 741 742 1098 1509 741
Size: 1 356 411 3602
Fragments arranged by size:
3602 411 356 1
EcoRI G'AATT_C
Cuts at: 1374 1374
Size: 4370
EcoRV GAT'ATC
Cuts at: 207 207
Size: 4370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 537 1107 1204 2389 537
Size: 570 97 1185 2518
Fragments arranged by size:
2518 1185 570 97
FspI TGC'GCA
Cuts at: 2151 2151
Size: 4370
HaeII r_GCGC'y
Cuts at: 444 567 796 941 1254 1804 2052 3074 4053
Size: 123 229 145 313 550 248 1022 979
Cuts at: 4053 444
Size: 761
Fragments arranged by size:
1022 979 761 550 313 248 229 145
123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1153 1185 1264 1296 2212 2242 2244 2274 1153
Size: 32 79 32 916 30 2 30 3249
Fragments arranged by size:
3249 916 79 32 32 30 30 2
HincII GTy'rAC
Cuts at: 344 1409 344
Size: 1065 3305
KasI G'GCGC_C
Cuts at: 1800 2048 1800
Size: 248 4122
KpnI G_GTAC'C
Cuts at: 1390 1390
Size: 4370
MluI A'CGCG_T
Cuts at: 3803 3803
Size: 4370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 872 3041 3225 872
Size: 2169 184 2017
Fragments arranged by size:
2169 2017 184
MscI TGG'CCA
Cuts at: 250 2131 250
Size: 1881 2489
MslI CAynn'nnrTG
Cuts at: 598 691 2359 2486 598
Size: 93 1668 127 2482
Fragments arranged by size:
2482 1668 127 93
MspA1I CmG'CkG
Cuts at: 219 246 491 902 1320 1481 1795 2155 3168
Size: 27 245 411 418 161 314 360 1013
Cuts at: 3168 3413 219
Size: 245 1176
Fragments arranged by size:
1176 1013 418 411 360 314 245 245
161 27
NaeI GCC'GGC
Cuts at: 942 2551 942
Size: 1609 2761
NarI GG'CG_CC
Cuts at: 1801 2049 1801
Size: 248 4122
NcoI C'CATG_G
Cuts at: 107 692 107
Size: 585 3785
NgoMIV G'CCGG_C
Cuts at: 940 2549 940
Size: 1609 2761
NotI GC'GGCC_GC
Cuts at: 903 903
Size: 4370
NspI r_CATG'y
Cuts at: 330 1423 1923 2830 330
Size: 1093 500 907 1870
Fragments arranged by size:
1870 1093 907 500
PciI A'CATG_T
Cuts at: 2826 2826
Size: 4370
PflMI CCAn_nnn'nTGG
Cuts at: 330 330
Size: 4370
PfoI T'CCnGG_A
Cuts at: 568 568
Size: 4370
PmeI GTTT'AAAC
Cuts at: 1369 1369
Size: 4370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 3509
Size: 32 4338
PpuMI rG'GwC_Cy
Cuts at: 1098 1098
Size: 4370
PspOMI G'GGCC_C
Cuts at: 741 741
Size: 4370
PstI C_TGCA'G
Cuts at: 239 398 1417 2102 239
Size: 159 1019 685 2507
Fragments arranged by size:
2507 1019 685 159
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4370
PvuII CAG'CTG
Cuts at: 1795 2155 1795
Size: 360 4010
RsrII CG'GwC_CG
Cuts at: 2565 2565
Size: 4370
SacI G_AGCT'C
Cuts at: 289 701 1384 289
Size: 412 683 3275
Fragments arranged by size:
3275 683 412
SacII CC_GC'GG
Cuts at: 1321 1321
Size: 4370
SalI G'TCGA_C
Cuts at: 1407 1407
Size: 4370
SapI GCTCTTCn'nnn_
Cuts at: 616 1115 2393 2603 616
Size: 499 1278 210 2383
Fragments arranged by size:
2383 1278 499 210
SbfI CC_TGCA'GG
Cuts at: 1417 1417
Size: 4370
ScaI AGT'ACT
Cuts at: 3887 3887
Size: 4370
SfcI C'TryA_G
Cuts at: 33 235 394 1114 1413 2098 3091 3282 33
Size: 202 159 720 299 685 993 191 1121
Fragments arranged by size:
1121 993 720 685 299 202 191 159
SfoI GGC'GCC
Cuts at: 1802 2050 1802
Size: 248 4122
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
SmaI CCC'GGG
Cuts at: 1392 1392
Size: 4370
SmlI C'TyrA_G
Cuts at: 724 1525 2440 2932 3194 724
Size: 801 915 492 262 1900
Fragments arranged by size:
1900 915 801 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4370
SphI G_CATG'C
Cuts at: 1423 1923 1423
Size: 500 3870
StyI C'CwwG_G
Cuts at: 107 692 1504 1858 107
Size: 585 812 354 2619
Fragments arranged by size:
2619 812 585 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1953 2086 2545 2754 1953
Size: 133 459 209 3569
Fragments arranged by size:
3569 459 209 133
TatI w'GTAC_w
Cuts at: 797 3885 797
Size: 3088 1282
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3887 3919 3887
Size: 32 4338
Tth111I GACn'n_nGTC
Cuts at: 1311 2167 1311
Size: 856 3514
XbaI T'CTAG_A
Cuts at: 1401 1401
Size: 4370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 699 855 1865 699
Size: 156 1010 3204
Fragments arranged by size:
3204 1010 156
XmaI C'CCGG_G
Cuts at: 1390 1390
Size: 4370
ZraI GAC'GTC
Cuts at: 681 837 681
Size: 156 4214
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AflII AleI AlfI AloI AscI AvrII BaeI BbvCI
BcgI BfrBI BmtI BsaXI BsiWI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NheI NruI NsiI PacI
PasI PmlI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI