Restriction map for
pF1KE0784
(Circular) (Six-base) MAPSORT of: pF1KE0784.seq Check: 5318 from: 1 to: 4382
pF1KE0784 4382 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AccI GT'mk_AC
Cuts at: 402 1420 402
Size: 1018 3364
Acc65I G'GTAC_C
Cuts at: 1398 1398
Size: 4382
AclI AA'CG_TT
Cuts at: 4222 4222
Size: 4382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1295 2207 2639 3386 3654 1295
Size: 912 432 747 268 2023
Fragments arranged by size:
2023 912 747 432 268
AflII C'TTAA_G
Cuts at: 1017 1017
Size: 4382
AflIII A'CryG_T
Cuts at: 1319 1734 2838 3815 1319
Size: 415 1104 977 1886
Fragments arranged by size:
1886 1104 977 415
AgeI A'CCGG_T
Cuts at: 2770 2770
Size: 4382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1145 1145
Size: 4382
AlwNI CAG_nnn'CTG
Cuts at: 1687 3254 1687
Size: 1567 2815
ApaLI G'TGCA_C
Cuts at: 3152 3152
Size: 4382
ApoI r'AATT_y
Cuts at: 291 942 1386 3735 3991 291
Size: 651 444 2349 256 682
Fragments arranged by size:
2349 682 651 444 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4382
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 185 1402 185
Size: 1217 3165
BamHI G'GATC_C
Cuts at: 1407 1407
Size: 4382
BanI G'GyrC_C
Cuts at: 1398 1812 2060 2095 1398
Size: 414 248 35 3685
Fragments arranged by size:
3685 414 248 35
BanII G_rGCy'C
Cuts at: 332 1396 2426 332
Size: 1064 1030 2288
Fragments arranged by size:
2288 1064 1030
BbeI G_GCGC'C
Cuts at: 1816 2064 1816
Size: 248 4134
BbsI GAAGACnn'nnnn_
Cuts at: 191 485 838 191
Size: 294 353 3735
Fragments arranged by size:
3735 353 294
BciVI GTATCCnnnnn_n'
Cuts at: 2280 3041 4382 2280
Size: 761 1341 2280
Fragments arranged by size:
2280 1341 761
BclI T'GATC_A
Cuts at: 1902 1902
Size: 4382
BglII A'GATC_T
Cuts at: 1897 3934 1897
Size: 2037 2345
BlpI GC'TnA_GC
Cuts at: 1494 1494
Size: 4382
Bme1580I G_kGCm'C
Cuts at: 2007 2100 3156 2007
Size: 93 1056 3233
Fragments arranged by size:
3233 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 203 1594 1675 2010 4154 203
Size: 1391 81 335 2144 431
Fragments arranged by size:
2144 1391 431 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 4382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 413 1088 230
Size: 183 675 3524
Fragments arranged by size:
3524 675 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 392 1558 2437 2929 3227 392
Size: 1166 879 492 298 1547
Fragments arranged by size:
1547 1166 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4086 4086
Size: 4382
BsaAI yAC'GTr
Cuts at: 1735 1735
Size: 4382
BsaBI GATnn'nnATC
Cuts at: 6 1896 3933 6
Size: 1890 2037 455
Fragments arranged by size:
2037 1890 455
BsaHI Gr'CG_yC
Cuts at: 1813 2061 2763 4259 1813
Size: 248 702 1496 1936
Fragments arranged by size:
1936 1496 702 248
BsaWI w'CCGG_w
Cuts at: 690 1571 2092 2770 3044 3191 3602 690
Size: 881 521 678 274 147 411 1470
Fragments arranged by size:
1470 881 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 462 638 1807 2565 3142 3638 462
Size: 176 1169 758 577 496 1206
Fragments arranged by size:
1206 1169 758 577 496 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1133 1133
Size: 4382
BsiEI CG_ry'CG
Cuts at: 104 172 1924 1970 3178 104
Size: 68 1752 46 1208 1308
Fragments arranged by size:
1752 1308 1208 68 46
BsiHKAI G_wGCw'C
Cuts at: 348 1396 2174 2364 3156 348
Size: 1048 778 190 792 1574
Fragments arranged by size:
1574 1048 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 788 788
Size: 4382
Bsp1286I G_dGCh'C
Cuts at: 332 348 1396 2007 2100 2174 2364 2426 3156
Size: 16 1048 611 93 74 190 62 730
Cuts at: 3156 332
Size: 1558
Fragments arranged by size:
1558 1048 730 611 190 93 74 62
16
BspEI T'CCGG_A
Cuts at: 690 3602 690
Size: 2912 1470
BspHI T'CATG_A
Cuts at: 527 3558 527
Size: 3031 1351
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1432 1646 1948 2329 1432
Size: 214 302 381 3485
Fragments arranged by size:
3485 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2674 2728 4212 4377 50
Size: 2624 54 1484 165 55
Fragments arranged by size:
2624 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2294 2294
Size: 4382
BsrFI r'CCGG_y
Cuts at: 2380 2561 2770 2380
Size: 181 209 3992
Fragments arranged by size:
3992 209 181
BssSI C'ACGA_G
Cuts at: 2653 3011 2653
Size: 358 4024
BstAPI GCAn_nnn'nTGC
Cuts at: 1759 1759
Size: 4382
BstBI TT'CG_AA
Cuts at: 2743 2743
Size: 4382
BstYI r'GATC_y
Cuts at: 1 388 1125 1407 1874 1897 2232 2478 3490
Size: 387 737 282 467 23 335 246 1012
Cuts at: 3490 3576 3588 3934 1
Size: 86 12 346 449
Fragments arranged by size:
1012 737 467 449 387 346 335 282
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 403 403
Size: 4382
BtgI C'CryG_G
Cuts at: 107 107
Size: 4382
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2332 2513 2676 2332
Size: 181 163 4038
Fragments arranged by size:
4038 181 163
BtsI GCAGTG_nn'
Cuts at: 1625 1625
Size: 4382
DraI TTT'AAA
Cuts at: 1381 1381
Size: 4382
DrdI GACnn_nn'nnGTC
Cuts at: 164 2088 2946 164
Size: 1924 858 1600
Fragments arranged by size:
1924 1600 858
EaeI y'GGCC_r
Cuts at: 1967 2141 2532 2559 3904 1967
Size: 174 391 27 1345 2445
Fragments arranged by size:
2445 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1967 1967
Size: 4382
EarI CTCTTCn'nnn_
Cuts at: 786 2405 2615 786
Size: 1619 210 2553
Fragments arranged by size:
2553 1619 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2590 2900 3046 4192 2590
Size: 310 146 1146 2780
Fragments arranged by size:
2780 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1394 1394
Size: 4382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 413 1088 1295 2207 2639 3386 3654 230
Size: 183 675 207 912 432 747 268 958
Fragments arranged by size:
958 912 747 675 432 268 207 183
EcoNI CCTnn'n_nnAGG
Cuts at: 854 854
Size: 4382
EcoO109I rG'GnC_Cy
Cuts at: 466 1521 466
Size: 1055 3327
EcoRI G'AATT_C
Cuts at: 1386 1386
Size: 4382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4382
FspI TGC'GCA
Cuts at: 2163 2163
Size: 4382
HaeII r_GCGC'y
Cuts at: 1371 1816 2064 3086 4065 1371
Size: 445 248 1022 979 1688
Fragments arranged by size:
1688 1022 979 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 828 860 2224 2254 2256 2286 828
Size: 32 1364 30 2 30 2924
Fragments arranged by size:
2924 1364 32 30 30 2
HincII GTy'rAC
Cuts at: 1421 1421
Size: 4382
HindIII A'AGCT_T
Cuts at: 806 1024 806
Size: 218 4164
KasI G'GCGC_C
Cuts at: 1812 2060 1812
Size: 248 4134
KpnI G_GTAC'C
Cuts at: 1402 1402
Size: 4382
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 204 222 305 3053 3237 204
Size: 18 83 2748 184 1349
Fragments arranged by size:
2748 1349 184 83 18
MscI TGG'CCA
Cuts at: 2143 2143
Size: 4382
MslI CAynn'nnrTG
Cuts at: 2371 2498 2371
Size: 127 4255
MspA1I CmG'CkG
Cuts at: 1493 1807 2167 3180 3425 1493
Size: 314 360 1013 245 2450
Fragments arranged by size:
2450 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2563 2563
Size: 4382
NarI GG'CG_CC
Cuts at: 1813 2061 1813
Size: 248 4134
NcoI C'CATG_G
Cuts at: 107 107
Size: 4382
NdeI CA'TA_TG
Cuts at: 818 818
Size: 4382
NgoMIV G'CCGG_C
Cuts at: 2561 2561
Size: 4382
NspI r_CATG'y
Cuts at: 1323 1435 1935 2842 1323
Size: 112 500 907 2863
Fragments arranged by size:
2863 907 500 112
PciI A'CATG_T
Cuts at: 1319 2838 1319
Size: 1519 2863
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 495 527 3521 3553 495
Size: 32 2994 32 1324
Fragments arranged by size:
2994 1324 32 32
PpuMI rG'GwC_Cy
Cuts at: 466 466
Size: 4382
PspXI vC'TCGA_Gb
Cuts at: 185 185
Size: 4382
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 568 600 568
Size: 32 4350
PstI C_TGCA'G
Cuts at: 1429 2114 1429
Size: 685 3697
PvuI CG_AT'CG
Cuts at: 104 172 104
Size: 68 4314
PvuII CAG'CTG
Cuts at: 1807 2167 1807
Size: 360 4022
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 1396 1396
Size: 4382
SalI G'TCGA_C
Cuts at: 1419 1419
Size: 4382
SapI GCTCTTCn'nnn_
Cuts at: 2405 2615 2405
Size: 210 4172
SbfI CC_TGCA'GG
Cuts at: 1429 1429
Size: 4382
ScaI AGT'ACT
Cuts at: 3899 3899
Size: 4382
SexAI A'CCwGG_T
Cuts at: 1190 1190
Size: 4382
SfcI C'TryA_G
Cuts at: 33 1425 2110 3103 3294 33
Size: 1392 685 993 191 1121
Fragments arranged by size:
1392 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1814 2062 1814
Size: 248 4134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SmaI CCC'GGG
Cuts at: 1404 1404
Size: 4382
SmlI C'TyrA_G
Cuts at: 185 407 1017 1537 2452 2944 3206 185
Size: 222 610 520 915 492 262 1361
Fragments arranged by size:
1361 915 610 520 492 262 222
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4382
SphI G_CATG'C
Cuts at: 1435 1935 1435
Size: 500 3882
StyI C'CwwG_G
Cuts at: 107 850 1516 1870 107
Size: 743 666 354 2619
Fragments arranged by size:
2619 743 666 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1099 1272 1965 2098 2557 2766 1099
Size: 173 693 133 459 209 2715
Fragments arranged by size:
2715 693 459 209 173 133
TatI w'GTAC_w
Cuts at: 3897 3897
Size: 4382
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3899 3931 3899
Size: 32 4350
Tth111I GACn'n_nGTC
Cuts at: 2179 2179
Size: 4382
XbaI T'CTAG_A
Cuts at: 1413 1413
Size: 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1877 1877
Size: 4382
XhoI C'TCGA_G
Cuts at: 185 185
Size: 4382
XmaI C'CCGG_G
Cuts at: 1402 1402
Size: 4382
XmnI GAAnn'nnTTC
Cuts at: 507 944 507
Size: 437 3945
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PmeI PpiI PpuMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsiWI BsmI BsrGI BssHII BstEII
BstXI Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI ZraI