Restriction map for
pF1KE0783
(Circular) (Six-base) MAPSORT of: pF1KE0783.seq Check: 2350 from: 1 to: 4169
pF1KE0783 4169 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1207 1207
Size: 4169
Acc65I G'GTAC_C
Cuts at: 1185 1185
Size: 4169
AclI AA'CG_TT
Cuts at: 4009 4009
Size: 4169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1994 2426 3173 3441 1994
Size: 432 747 268 2722
Fragments arranged by size:
2722 747 432 268
AflIII A'CryG_T
Cuts at: 1521 2625 3602 1521
Size: 1104 977 2088
Fragments arranged by size:
2088 1104 977
AgeI A'CCGG_T
Cuts at: 2557 2557
Size: 4169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 481 481
Size: 4169
AlwNI CAG_nnn'CTG
Cuts at: 264 338 841 1102 1474 3041 264
Size: 74 503 261 372 1567 1392
Fragments arranged by size:
1567 1392 503 372 261 74
ApaLI G'TGCA_C
Cuts at: 163 2939 163
Size: 2776 1393
ApoI r'AATT_y
Cuts at: 1173 3522 3778 1173
Size: 2349 256 1564
Fragments arranged by size:
2349 1564 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
AvaI C'yCGr_G
Cuts at: 202 385 739 885 1189 202
Size: 183 354 146 304 3182
Fragments arranged by size:
3182 354 304 183 146
BamHI G'GATC_C
Cuts at: 268 492 876 906 1194 268
Size: 224 384 30 288 3243
Fragments arranged by size:
3243 384 288 224 30
BanI G'GyrC_C
Cuts at: 951 1034 1185 1599 1847 1882 951
Size: 83 151 414 248 35 3238
Fragments arranged by size:
3238 414 248 151 83 35
BanII G_rGCy'C
Cuts at: 1056 1183 2213 1056
Size: 127 1030 3012
Fragments arranged by size:
3012 1030 127
BbeI G_GCGC'C
Cuts at: 1603 1851 1603
Size: 248 3921
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 535 569 535
Size: 34 4135
BciVI GTATCCnnnnn_n'
Cuts at: 2067 2828 4169 2067
Size: 761 1341 2067
Fragments arranged by size:
2067 1341 761
BclI T'GATC_A
Cuts at: 1689 1689
Size: 4169
BglI GCCn_nnn'nGGC
Cuts at: 302 483 302
Size: 181 3988
BglII A'GATC_T
Cuts at: 1684 3721 1684
Size: 2037 2132
BlpI GC'TnA_GC
Cuts at: 1281 1281
Size: 4169
Bme1580I G_kGCm'C
Cuts at: 167 1794 1887 2943 167
Size: 1627 93 1056 1393
Fragments arranged by size:
1627 1393 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 274 1381 1462 1797 3941 274
Size: 1107 81 335 2144 502
Fragments arranged by size:
2144 1107 502 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 600 2536 600
Size: 1936 2233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 738 271
Size: 467 3702
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 1345 2224 2716 3014 210
Size: 1135 879 492 298 1365
Fragments arranged by size:
1365 1135 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 694 3873 694
Size: 3179 990
BsaAI yAC'GTr
Cuts at: 1522 1522
Size: 4169
BsaBI GATnn'nnATC
Cuts at: 6 1683 3720 6
Size: 1677 2037 455
Fragments arranged by size:
2037 1677 455
BsaHI Gr'CG_yC
Cuts at: 416 1600 1848 2550 4046 416
Size: 1184 248 702 1496 539
Fragments arranged by size:
1496 1184 702 539 248
BsaWI w'CCGG_w
Cuts at: 815 847 1358 1879 2557 2831 2978 3389 815
Size: 32 511 521 678 274 147 411 1595
Fragments arranged by size:
1595 678 521 511 411 274 147 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 298 328 298
Size: 30 4139
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 592 735 1138 592
Size: 143 403 3623
Fragments arranged by size:
3623 403 143
BseYI C'CCAG_C
Cuts at: 1594 2352 2929 3425 1594
Size: 758 577 496 2338
Fragments arranged by size:
2338 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1711 1757 2965 104
Size: 1607 46 1208 1308
Fragments arranged by size:
1607 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 167 1085 1183 1961 2151 2943 167
Size: 918 98 778 190 792 1393
Fragments arranged by size:
1393 918 792 778 190 98
Bsp1286I G_dGCh'C
Cuts at: 167 1056 1085 1183 1794 1887 1961 2151 2213
Size: 889 29 98 611 93 74 190 62
Cuts at: 2213 2943 167
Size: 730 1393
Fragments arranged by size:
1393 889 730 611 190 98 93 74
62 29
BspEI T'CCGG_A
Cuts at: 815 3389 815
Size: 2574 1595
BspHI T'CATG_A
Cuts at: 377 3345 377
Size: 2968 1201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1219 1433 1735 2116 1219
Size: 214 302 381 3272
Fragments arranged by size:
3272 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2461 2515 3999 4164 50
Size: 2411 54 1484 165 55
Fragments arranged by size:
2411 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2081 2081
Size: 4169
BsrFI r'CCGG_y
Cuts at: 2167 2348 2557 2167
Size: 181 209 3779
Fragments arranged by size:
3779 209 181
BssSI C'ACGA_G
Cuts at: 2440 2798 2440
Size: 358 3811
BstAPI GCAn_nnn'nTGC
Cuts at: 338 1546 338
Size: 1208 2961
BstBI TT'CG_AA
Cuts at: 2530 2530
Size: 4169
BstEII G'GTnAC_C
Cuts at: 423 1011 423
Size: 588 3581
BstXI CCAn_nnnn'nTGG
Cuts at: 540 540
Size: 4169
BstYI r'GATC_y
Cuts at: 1 268 492 516 820 876 906 1194 1661
Size: 267 224 24 304 56 30 288 467
Cuts at: 1661 1684 2019 2265 3277 3363 3375 3721 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 304 288 267
246 224 86 56 30 24 23 12
Bsu36I CC'TnA_GG
Cuts at: 575 575
Size: 4169
BtgI C'CryG_G
Cuts at: 145 213 824 145
Size: 68 611 3490
Fragments arranged by size:
3490 611 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2119 2300 2463 2119
Size: 181 163 3825
Fragments arranged by size:
3825 181 163
BtsI GCAGTG_nn'
Cuts at: 545 1412 545
Size: 867 3302
DraI TTT'AAA
Cuts at: 1168 1168
Size: 4169
DrdI GACnn_nn'nnGTC
Cuts at: 421 967 1875 2733 421
Size: 546 908 858 1857
Fragments arranged by size:
1857 908 858 546
EaeI y'GGCC_r
Cuts at: 542 917 1754 1928 2319 2346 3691 542
Size: 375 837 174 391 27 1345 1020
Fragments arranged by size:
1345 1020 837 391 375 174 27
EagI C'GGCC_G
Cuts at: 1754 1754
Size: 4169
EarI CTCTTCn'nnn_
Cuts at: 2192 2402 2192
Size: 210 3959
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2377 2687 2833 3979 2377
Size: 310 146 1146 2567
Fragments arranged by size:
2567 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1181 1181
Size: 4169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 738 1994 2426 3173 3441 271
Size: 467 1256 432 747 268 999
Fragments arranged by size:
1256 999 747 467 432 268
EcoO109I rG'GnC_Cy
Cuts at: 198 838 969 1308 198
Size: 640 131 339 3059
Fragments arranged by size:
3059 640 339 131
EcoRI G'AATT_C
Cuts at: 1173 1173
Size: 4169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 4169
FspI TGC'GCA
Cuts at: 1950 1950
Size: 4169
HaeII r_GCGC'y
Cuts at: 1603 1851 2873 3852 1603
Size: 248 1022 979 1920
Fragments arranged by size:
1920 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 260 292 868 900 2011 2041 2043 2073 260
Size: 32 576 32 1111 30 2 30 2356
Fragments arranged by size:
2356 1111 576 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1208 1208
Size: 4169
KasI G'GCGC_C
Cuts at: 1599 1847 1599
Size: 248 3921
KpnI G_GTAC'C
Cuts at: 1189 1189
Size: 4169
MfeI C'AATT_G
Cuts at: 446 446
Size: 4169
MluI A'CGCG_T
Cuts at: 3602 3602
Size: 4169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 557 2840 3024 557
Size: 2283 184 1702
Fragments arranged by size:
2283 1702 184
MscI TGG'CCA
Cuts at: 919 1930 919
Size: 1011 3158
MslI CAynn'nnrTG
Cuts at: 172 296 1009 2158 2285 172
Size: 124 713 1149 127 2056
Fragments arranged by size:
2056 1149 713 127 124
MspA1I CmG'CkG
Cuts at: 183 335 1001 1280 1594 1954 2967 3212 183
Size: 152 666 279 314 360 1013 245 1140
Fragments arranged by size:
1140 1013 666 360 314 279 245 152
NaeI GCC'GGC
Cuts at: 2350 2350
Size: 4169
NarI GG'CG_CC
Cuts at: 1600 1848 1600
Size: 248 3921
NcoI C'CATG_G
Cuts at: 213 213
Size: 4169
NgoMIV G'CCGG_C
Cuts at: 2348 2348
Size: 4169
NspI r_CATG'y
Cuts at: 1222 1722 2629 1222
Size: 500 907 2762
Fragments arranged by size:
2762 907 500
PasI CC'CwG_GG
Cuts at: 841 1018 1153 841
Size: 177 135 3857
Fragments arranged by size:
3857 177 135
PciI A'CATG_T
Cuts at: 2625 2625
Size: 4169
PflMI CCAn_nnn'nTGG
Cuts at: 348 1092 348
Size: 744 3425
PfoI T'CCnGG_A
Cuts at: 1118 1118
Size: 4169
PmeI GTTT'AAAC
Cuts at: 1168 1168
Size: 4169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 4137
PpuMI rG'GwC_Cy
Cuts at: 198 969 198
Size: 771 3398
PstI C_TGCA'G
Cuts at: 484 1216 1901 484
Size: 732 685 2752
Fragments arranged by size:
2752 732 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4169
PvuII CAG'CTG
Cuts at: 183 335 1001 1594 1954 183
Size: 152 666 593 360 2398
Fragments arranged by size:
2398 666 593 360 152
RsrII CG'GwC_CG
Cuts at: 2364 2364
Size: 4169
SacI G_AGCT'C
Cuts at: 1183 1183
Size: 4169
SalI G'TCGA_C
Cuts at: 1206 1206
Size: 4169
SanDI GG'GwC_CC
Cuts at: 198 198
Size: 4169
SapI GCTCTTCn'nnn_
Cuts at: 2192 2402 2192
Size: 210 3959
SbfI CC_TGCA'GG
Cuts at: 484 1216 484
Size: 732 3437
ScaI AGT'ACT
Cuts at: 3686 3686
Size: 4169
SfcI C'TryA_G
Cuts at: 33 480 1212 1897 2890 3081 33
Size: 447 732 685 993 191 1121
Fragments arranged by size:
1121 993 732 685 447 191
SfoI GGC'GCC
Cuts at: 1601 1849 1601
Size: 248 3921
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
SmaI CCC'GGG
Cuts at: 387 1191 387
Size: 804 3365
SmlI C'TyrA_G
Cuts at: 225 1324 2239 2731 2993 225
Size: 1099 915 492 262 1401
Fragments arranged by size:
1401 1099 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4169
SphI G_CATG'C
Cuts at: 1222 1722 1222
Size: 500 3669
StuI AGG'CCT
Cuts at: 583 583
Size: 4169
StyI C'CwwG_G
Cuts at: 213 656 1303 1657 213
Size: 443 647 354 2725
Fragments arranged by size:
2725 647 443 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 984 1752 1885 2344 2553 984
Size: 768 133 459 209 2600
Fragments arranged by size:
2600 768 459 209 133
TatI w'GTAC_w
Cuts at: 3684 3684
Size: 4169
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4137
Tth111I GACn'n_nGTC
Cuts at: 196 1966 196
Size: 1770 2399
XbaI T'CTAG_A
Cuts at: 1200 1200
Size: 4169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 152 1664 152
Size: 1512 2657
XmaI C'CCGG_G
Cuts at: 385 1189 385
Size: 804 3365
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI