(Circular) (Six-base) MAPSORT of: pF1KE0779.seq Check: 809 from: 1 to: 4157 pF1KE0779 4157 bp With 182 enzymes: SgfI * October 7, 2009 14:27 .. AccI GT'mk_AC Cuts at: 1195 1195 Size: 4157 Acc65I G'GTAC_C Cuts at: 1173 1173 Size: 4157 AclI AA'CG_TT Cuts at: 3997 3997 Size: 4157 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 397 679 1982 2414 3161 3429 397 Size: 282 1303 432 747 268 1125 Fragments arranged by size: 1303 1125 747 432 282 268 AfeI AGC'GCT Cuts at: 1056 1056 Size: 4157 AflIII A'CryG_T Cuts at: 1509 2613 3590 1509 Size: 1104 977 2076 Fragments arranged by size: 2076 1104 977 AgeI A'CCGG_T Cuts at: 2545 2545 Size: 4157 AlwNI CAG_nnn'CTG Cuts at: 264 1462 3029 264 Size: 1198 1567 1392 Fragments arranged by size: 1567 1392 1198 ApaLI G'TGCA_C Cuts at: 586 2927 586 Size: 2341 1816 ApoI r'AATT_y Cuts at: 1161 3510 3766 1161 Size: 2349 256 1552 Fragments arranged by size: 2349 1552 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4157 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4157 AvaI C'yCGr_G Cuts at: 784 811 965 1177 784 Size: 27 154 212 3764 Fragments arranged by size: 3764 212 154 27 BamHI G'GATC_C Cuts at: 1182 1182 Size: 4157 BanI G'GyrC_C Cuts at: 1085 1173 1587 1835 1870 1085 Size: 88 414 248 35 3372 Fragments arranged by size: 3372 414 248 88 35 BanII G_rGCy'C Cuts at: 203 290 440 524 791 1171 2201 203 Size: 87 150 84 267 380 1030 2159 Fragments arranged by size: 2159 1030 380 267 150 87 84 BbeI G_GCGC'C Cuts at: 1089 1591 1839 1089 Size: 502 248 3407 Fragments arranged by size: 3407 502 248 BbvCI CC'TCA_GC Cuts at: 977 977 Size: 4157 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 134 168 468 502 90 Size: 34 10 34 300 34 3745 Fragments arranged by size: 3745 300 34 34 34 10 BciVI GTATCCnnnnn_n' Cuts at: 2055 2816 4157 2055 Size: 761 1341 2055 Fragments arranged by size: 2055 1341 761 BclI T'GATC_A Cuts at: 1677 1677 Size: 4157 BglI GCCn_nnn'nGGC Cuts at: 607 1003 607 Size: 396 3761 BglII A'GATC_T Cuts at: 1672 3709 1672 Size: 2037 2120 BlpI GC'TnA_GC Cuts at: 1269 1269 Size: 4157 Bme1580I G_kGCm'C Cuts at: 590 1782 1875 2931 590 Size: 1192 93 1056 1816 Fragments arranged by size: 1816 1192 1056 93 BmgBI CAC'GTC Cuts at: 401 401 Size: 4157 BmrI ACTGGGnnnn_n' Cuts at: 1369 1450 1785 3929 1369 Size: 81 335 2144 1597 Fragments arranged by size: 2144 1597 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2524 2524 Size: 4157 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 187 253 640 1113 187 Size: 66 387 473 3231 Fragments arranged by size: 3231 473 387 66 Bpu10I CC'TnA_GC Cuts at: 977 977 Size: 4157 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1333 2212 2704 3002 1333 Size: 879 492 298 2488 Fragments arranged by size: 2488 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3861 3861 Size: 4157 BsaAI yAC'GTr Cuts at: 1510 1510 Size: 4157 BsaBI GATnn'nnATC Cuts at: 6 1066 1671 3708 6 Size: 1060 605 2037 455 Fragments arranged by size: 2037 1060 605 455 BsaHI Gr'CG_yC Cuts at: 842 857 952 1086 1588 1836 2538 4034 842 Size: 15 95 134 502 248 702 1496 965 Fragments arranged by size: 1496 965 702 502 248 134 95 15 BsaWI w'CCGG_w Cuts at: 1346 1867 2545 2819 2966 3377 1346 Size: 521 678 274 147 411 2126 Fragments arranged by size: 2126 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 181 184 211 865 1065 1153 181 Size: 3 27 654 200 88 3185 Fragments arranged by size: 3185 654 200 88 27 3 BseYI C'CCAG_C Cuts at: 444 602 852 1144 1582 2340 2917 3413 444 Size: 158 250 292 438 758 577 496 1188 Fragments arranged by size: 1188 758 577 496 438 292 250 158 BsiEI CG_ry'CG Cuts at: 104 1007 1699 1745 2953 104 Size: 903 692 46 1208 1308 Fragments arranged by size: 1308 1208 903 692 46 BsiHKAI G_wGCw'C Cuts at: 203 258 290 590 791 1079 1171 1949 2139 Size: 55 32 300 201 288 92 778 190 Cuts at: 2139 2931 203 Size: 792 1429 Fragments arranged by size: 1429 792 778 300 288 201 190 92 55 32 Bsp1286I G_dGCh'C Cuts at: 203 258 290 440 524 590 791 1079 1171 Size: 55 32 150 84 66 201 288 92 Cuts at: 1171 1782 1875 1949 2139 2201 2931 203 Size: 611 93 74 190 62 730 1429 Fragments arranged by size: 1429 730 611 288 201 190 150 93 92 84 74 66 62 55 32 BspEI T'CCGG_A Cuts at: 3377 3377 Size: 4157 BspHI T'CATG_A Cuts at: 3333 3333 Size: 4157 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1140 1207 1421 1723 2104 1140 Size: 67 214 302 381 3193 Fragments arranged by size: 3193 381 302 214 67 BsrBI CCG'CTC Cuts at: 50 807 1129 2449 2503 3987 4152 50 Size: 757 322 1320 54 1484 165 55 Fragments arranged by size: 1484 1320 757 322 165 55 54 BsrDI GCAATG_nn' Cuts at: 2069 2069 Size: 4157 BsrFI r'CCGG_y Cuts at: 135 695 899 2155 2336 2545 135 Size: 560 204 1256 181 209 1747 Fragments arranged by size: 1747 1256 560 209 204 181 BssHII G'CGCG_C Cuts at: 227 449 794 227 Size: 222 345 3590 Fragments arranged by size: 3590 345 222 BssSI C'ACGA_G Cuts at: 726 2428 2786 726 Size: 1702 358 2097 Fragments arranged by size: 2097 1702 358 BstAPI GCAn_nnn'nTGC Cuts at: 1534 1534 Size: 4157 BstBI TT'CG_AA Cuts at: 2518 2518 Size: 4157 BstEII G'GTnAC_C Cuts at: 342 342 Size: 4157 BstYI r'GATC_y Cuts at: 1 1182 1649 1672 2007 2253 3265 3351 3363 Size: 1181 467 23 335 246 1012 86 12 Cuts at: 3363 3709 1 Size: 346 449 Fragments arranged by size: 1181 1012 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 408 581 636 1001 408 Size: 173 55 365 3564 Fragments arranged by size: 3564 365 173 55 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2107 2288 2451 2107 Size: 181 163 3813 Fragments arranged by size: 3813 181 163 BtsI GCAGTG_nn' Cuts at: 1127 1400 1127 Size: 273 3884 DraI TTT'AAA Cuts at: 1156 1156 Size: 4157 DrdI GACnn_nn'nnGTC Cuts at: 1863 2721 1863 Size: 858 3299 EaeI y'GGCC_r Cuts at: 327 720 755 908 1004 1742 1916 2307 2334 Size: 393 35 153 96 738 174 391 27 Cuts at: 2334 3679 327 Size: 1345 805 Fragments arranged by size: 1345 805 738 393 391 174 153 96 35 27 EagI C'GGCC_G Cuts at: 1004 1742 1004 Size: 738 3419 EarI CTCTTCn'nnn_ Cuts at: 871 1130 2180 2390 871 Size: 259 1050 210 2638 Fragments arranged by size: 2638 1050 259 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 154 603 1129 2365 2675 2821 3967 154 Size: 449 526 1236 310 146 1146 344 Fragments arranged by size: 1236 1146 526 449 344 310 146 EcoICRI GAG'CTC Cuts at: 201 288 789 1169 201 Size: 87 501 380 3189 Fragments arranged by size: 3189 501 380 87 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 187 253 397 640 679 1113 1982 2414 3161 Size: 66 144 243 39 434 869 432 747 Cuts at: 3161 3429 187 Size: 268 915 Fragments arranged by size: 915 869 747 434 432 268 243 144 66 39 EcoNI CCTnn'n_nnAGG Cuts at: 313 910 313 Size: 597 3560 EcoO109I rG'GnC_Cy Cuts at: 537 654 816 1296 537 Size: 117 162 480 3398 Fragments arranged by size: 3398 480 162 117 EcoRI G'AATT_C Cuts at: 1161 1161 Size: 4157 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2176 2176 Size: 4157 FspI TGC'GCA Cuts at: 1938 1938 Size: 4157 HaeII r_GCGC'y Cuts at: 702 853 1058 1089 1591 1839 2861 3840 702 Size: 151 205 31 502 248 1022 979 1019 Fragments arranged by size: 1022 1019 979 502 248 205 151 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1999 2029 2031 2061 1999 Size: 30 2 30 4095 Fragments arranged by size: 4095 30 30 2 HincII GTy'rAC Cuts at: 1196 1196 Size: 4157 KasI G'GCGC_C Cuts at: 1085 1587 1835 1085 Size: 502 248 3407 Fragments arranged by size: 3407 502 248 KpnI G_GTAC'C Cuts at: 1177 1177 Size: 4157 MluI A'CGCG_T Cuts at: 3590 3590 Size: 4157 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2828 3012 2828 Size: 184 3973 MscI TGG'CCA Cuts at: 1918 1918 Size: 4157 MslI CAynn'nnrTG Cuts at: 2146 2273 2146 Size: 127 4030 MspA1I CmG'CkG Cuts at: 583 593 606 638 1003 1101 1268 1582 1942 Size: 10 13 32 365 98 167 314 360 Cuts at: 1942 2955 3200 583 Size: 1013 245 1540 Fragments arranged by size: 1540 1013 365 360 314 245 167 98 32 13 10 NaeI GCC'GGC Cuts at: 137 697 2338 137 Size: 560 1641 1956 Fragments arranged by size: 1956 1641 560 NarI GG'CG_CC Cuts at: 1086 1588 1836 1086 Size: 502 248 3407 Fragments arranged by size: 3407 502 248 NgoMIV G'CCGG_C Cuts at: 135 695 2336 135 Size: 560 1641 1956 Fragments arranged by size: 1956 1641 560 NspI r_CATG'y Cuts at: 1210 1710 2617 1210 Size: 500 907 2750 Fragments arranged by size: 2750 907 500 PasI CC'CwG_GG Cuts at: 309 893 309 Size: 584 3573 PciI A'CATG_T Cuts at: 2613 2613 Size: 4157 PmeI GTTT'AAAC Cuts at: 1156 1156 Size: 4157 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3296 3328 3296 Size: 32 4125 PpuMI rG'GwC_Cy Cuts at: 537 654 537 Size: 117 4040 PshAI GACnn'nnGTC Cuts at: 265 265 Size: 4157 PstI C_TGCA'G Cuts at: 1204 1889 1204 Size: 685 3472 PvuI CG_AT'CG Cuts at: 104 104 Size: 4157 PvuII CAG'CTG Cuts at: 606 1582 1942 606 Size: 976 360 2821 Fragments arranged by size: 2821 976 360 RsrII CG'GwC_CG Cuts at: 2352 2352 Size: 4157 SacI G_AGCT'C Cuts at: 203 290 791 1171 203 Size: 87 501 380 3189 Fragments arranged by size: 3189 501 380 87 SacII CC_GC'GG Cuts at: 584 639 1004 584 Size: 55 365 3737 Fragments arranged by size: 3737 365 55 SalI G'TCGA_C Cuts at: 1194 1194 Size: 4157 SapI GCTCTTCn'nnn_ Cuts at: 871 2180 2390 871 Size: 1309 210 2638 Fragments arranged by size: 2638 1309 210 SbfI CC_TGCA'GG Cuts at: 1204 1204 Size: 4157 ScaI AGT'ACT Cuts at: 3674 3674 Size: 4157 SfcI C'TryA_G Cuts at: 33 504 1200 1885 2878 3069 33 Size: 471 696 685 993 191 1121 Fragments arranged by size: 1121 993 696 685 471 191 SfiI GGCCn_nnn'nGGCC Cuts at: 1003 1003 Size: 4157 SfoI GGC'GCC Cuts at: 1087 1589 1837 1087 Size: 502 248 3407 Fragments arranged by size: 3407 502 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4157 SmaI CCC'GGG Cuts at: 967 1179 967 Size: 212 3945 SmlI C'TyrA_G Cuts at: 1312 2227 2719 2981 1312 Size: 915 492 262 2488 Fragments arranged by size: 2488 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4157 SphI G_CATG'C Cuts at: 1210 1710 1210 Size: 500 3657 StuI AGG'CCT Cuts at: 732 732 Size: 4157 StyI C'CwwG_G Cuts at: 560 1291 1645 560 Size: 731 354 3072 Fragments arranged by size: 3072 731 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 361 727 1740 1873 2332 2541 361 Size: 366 1013 133 459 209 1977 Fragments arranged by size: 1977 1013 459 366 209 133 TatI w'GTAC_w Cuts at: 623 3672 623 Size: 3049 1108 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3674 3706 3674 Size: 32 4125 Tth111I GACn'n_nGTC Cuts at: 1954 1954 Size: 4157 XbaI T'CTAG_A Cuts at: 1188 1188 Size: 4157 XcmI CCAnnnn_n'nnnnTGG Cuts at: 322 1652 322 Size: 1330 2827 XmaI C'CCGG_G Cuts at: 965 1177 965 Size: 212 3945 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BfrBI BmtI BsaXI BsgI BsiWI BsmI BsmBI BsrGI BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PflMI PfoI PmlI PsiI PspOMI PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI