Restriction map for pF1KE0779
 (Circular) (Six-base) MAPSORT of: pF1KE0779.seq  Check: 809  from: 1  to: 4157



pF1KE0779  4157 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AccI GT'mk_AC

Cuts at:   1195    1195 
   Size:       4157
 
Acc65I G'GTAC_C

Cuts at:   1173    1173 
   Size:       4157
 
AclI AA'CG_TT

Cuts at:   3997    3997 
   Size:       4157
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    397     679    1982    2414    3161    3429     397 
   Size:        282    1303     432     747     268    1125

  Fragments arranged by size: 

                1303    1125     747     432     282     268
 
AfeI AGC'GCT

Cuts at:   1056    1056 
   Size:       4157
 
AflIII A'CryG_T

Cuts at:   1509    2613    3590    1509 
   Size:       1104     977    2076

  Fragments arranged by size: 

                2076    1104     977
 
AgeI A'CCGG_T

Cuts at:   2545    2545 
   Size:       4157
 
AlwNI CAG_nnn'CTG

Cuts at:    264    1462    3029     264 
   Size:       1198    1567    1392

  Fragments arranged by size: 

                1567    1392    1198
 
ApaLI G'TGCA_C

Cuts at:    586    2927     586 
   Size:       2341    1816
 
ApoI r'AATT_y

Cuts at:   1161    3510    3766    1161 
   Size:       2349     256    1552

  Fragments arranged by size: 

                2349    1552     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4157
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4157
 
AvaI C'yCGr_G

Cuts at:    784     811     965    1177     784 
   Size:         27     154     212    3764

  Fragments arranged by size: 

                3764     212     154      27
 
BamHI G'GATC_C

Cuts at:   1182    1182 
   Size:       4157
 
BanI G'GyrC_C

Cuts at:   1085    1173    1587    1835    1870    1085 
   Size:         88     414     248      35    3372

  Fragments arranged by size: 

                3372     414     248      88      35
 
BanII G_rGCy'C

Cuts at:    203     290     440     524     791    1171    2201     203 
   Size:         87     150      84     267     380    1030    2159

  Fragments arranged by size: 

                2159    1030     380     267     150      87      84
 
BbeI G_GCGC'C

Cuts at:   1089    1591    1839    1089 
   Size:        502     248    3407

  Fragments arranged by size: 

                3407     502     248
 
BbvCI CC'TCA_GC

Cuts at:    977     977 
   Size:       4157
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124     134     168     468     502      90 
   Size:         34      10      34     300      34    3745

  Fragments arranged by size: 

                3745     300      34      34      34      10
 
BciVI GTATCCnnnnn_n'

Cuts at:   2055    2816    4157    2055 
   Size:        761    1341    2055

  Fragments arranged by size: 

                2055    1341     761
 
BclI T'GATC_A

Cuts at:   1677    1677 
   Size:       4157
 
BglI GCCn_nnn'nGGC

Cuts at:    607    1003     607 
   Size:        396    3761
 
BglII A'GATC_T

Cuts at:   1672    3709    1672 
   Size:       2037    2120
 
BlpI GC'TnA_GC

Cuts at:   1269    1269 
   Size:       4157
 
Bme1580I G_kGCm'C

Cuts at:    590    1782    1875    2931     590 
   Size:       1192      93    1056    1816

  Fragments arranged by size: 

                1816    1192    1056      93
 
BmgBI CAC'GTC

Cuts at:    401     401 
   Size:       4157
 
BmrI ACTGGGnnnn_n'

Cuts at:   1369    1450    1785    3929    1369 
   Size:         81     335    2144    1597

  Fragments arranged by size: 

                2144    1597     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2524    2524 
   Size:       4157
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    187     253     640    1113     187 
   Size:         66     387     473    3231

  Fragments arranged by size: 

                3231     473     387      66
 
Bpu10I CC'TnA_GC

Cuts at:    977     977 
   Size:       4157
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1333    2212    2704    3002    1333 
   Size:        879     492     298    2488

  Fragments arranged by size: 

                2488     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3861    3861 
   Size:       4157
 
BsaAI yAC'GTr

Cuts at:   1510    1510 
   Size:       4157
 
BsaBI GATnn'nnATC

Cuts at:      6    1066    1671    3708       6 
   Size:       1060     605    2037     455

  Fragments arranged by size: 

                2037    1060     605     455
 
BsaHI Gr'CG_yC

Cuts at:    842     857     952    1086    1588    1836    2538    4034     842 
   Size:         15      95     134     502     248     702    1496     965

  Fragments arranged by size: 

                1496     965     702     502     248     134      95      15
            
 
BsaWI w'CCGG_w

Cuts at:   1346    1867    2545    2819    2966    3377    1346 
   Size:        521     678     274     147     411    2126

  Fragments arranged by size: 

                2126     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    181     184     211     865    1065    1153     181 
   Size:          3      27     654     200      88    3185

  Fragments arranged by size: 

                3185     654     200      88      27       3
 
BseYI C'CCAG_C

Cuts at:    444     602     852    1144    1582    2340    2917    3413     444 
   Size:        158     250     292     438     758     577     496    1188

  Fragments arranged by size: 

                1188     758     577     496     438     292     250     158
            
 
BsiEI CG_ry'CG

Cuts at:    104    1007    1699    1745    2953     104 
   Size:        903     692      46    1208    1308

  Fragments arranged by size: 

                1308    1208     903     692      46
 
BsiHKAI G_wGCw'C

Cuts at:    203     258     290     590     791    1079    1171    1949    2139 
   Size:         55      32     300     201     288      92     778     190

Cuts at:   2139    2931     203 
   Size:        792    1429

  Fragments arranged by size: 

                1429     792     778     300     288     201     190      92
                  55      32
 
Bsp1286I G_dGCh'C

Cuts at:    203     258     290     440     524     590     791    1079    1171 
   Size:         55      32     150      84      66     201     288      92

Cuts at:   1171    1782    1875    1949    2139    2201    2931     203 
   Size:        611      93      74     190      62     730    1429

  Fragments arranged by size: 

                1429     730     611     288     201     190     150      93
                  92      84      74      66      62      55      32
 
BspEI T'CCGG_A

Cuts at:   3377    3377 
   Size:       4157
 
BspHI T'CATG_A

Cuts at:   3333    3333 
   Size:       4157
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1140    1207    1421    1723    2104    1140 
   Size:         67     214     302     381    3193

  Fragments arranged by size: 

                3193     381     302     214      67
 
BsrBI CCG'CTC

Cuts at:     50     807    1129    2449    2503    3987    4152      50 
   Size:        757     322    1320      54    1484     165      55

  Fragments arranged by size: 

                1484    1320     757     322     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2069    2069 
   Size:       4157
 
BsrFI r'CCGG_y

Cuts at:    135     695     899    2155    2336    2545     135 
   Size:        560     204    1256     181     209    1747

  Fragments arranged by size: 

                1747    1256     560     209     204     181
 
BssHII G'CGCG_C

Cuts at:    227     449     794     227 
   Size:        222     345    3590

  Fragments arranged by size: 

                3590     345     222
 
BssSI C'ACGA_G

Cuts at:    726    2428    2786     726 
   Size:       1702     358    2097

  Fragments arranged by size: 

                2097    1702     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1534    1534 
   Size:       4157
 
BstBI TT'CG_AA

Cuts at:   2518    2518 
   Size:       4157
 
BstEII G'GTnAC_C

Cuts at:    342     342 
   Size:       4157
 
BstYI r'GATC_y

Cuts at:      1    1182    1649    1672    2007    2253    3265    3351    3363 
   Size:       1181     467      23     335     246    1012      86      12

Cuts at:   3363    3709       1 
   Size:        346     449

  Fragments arranged by size: 

                1181    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    408     581     636    1001     408 
   Size:        173      55     365    3564

  Fragments arranged by size: 

                3564     365     173      55
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2107    2288    2451    2107 
   Size:        181     163    3813

  Fragments arranged by size: 

                3813     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1127    1400    1127 
   Size:        273    3884
 
DraI TTT'AAA

Cuts at:   1156    1156 
   Size:       4157
 
DrdI GACnn_nn'nnGTC

Cuts at:   1863    2721    1863 
   Size:        858    3299
 
EaeI y'GGCC_r

Cuts at:    327     720     755     908    1004    1742    1916    2307    2334 
   Size:        393      35     153      96     738     174     391      27

Cuts at:   2334    3679     327 
   Size:       1345     805

  Fragments arranged by size: 

                1345     805     738     393     391     174     153      96
                  35      27
 
EagI C'GGCC_G

Cuts at:   1004    1742    1004 
   Size:        738    3419
 
EarI CTCTTCn'nnn_

Cuts at:    871    1130    2180    2390     871 
   Size:        259    1050     210    2638

  Fragments arranged by size: 

                2638    1050     259     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    154     603    1129    2365    2675    2821    3967     154 
   Size:        449     526    1236     310     146    1146     344

  Fragments arranged by size: 

                1236    1146     526     449     344     310     146
 
EcoICRI GAG'CTC

Cuts at:    201     288     789    1169     201 
   Size:         87     501     380    3189

  Fragments arranged by size: 

                3189     501     380      87
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    187     253     397     640     679    1113    1982    2414    3161 
   Size:         66     144     243      39     434     869     432     747

Cuts at:   3161    3429     187 
   Size:        268     915

  Fragments arranged by size: 

                 915     869     747     434     432     268     243     144
                  66      39
 
EcoNI CCTnn'n_nnAGG

Cuts at:    313     910     313 
   Size:        597    3560
 
EcoO109I rG'GnC_Cy

Cuts at:    537     654     816    1296     537 
   Size:        117     162     480    3398

  Fragments arranged by size: 

                3398     480     162     117
 
EcoRI G'AATT_C

Cuts at:   1161    1161 
   Size:       4157
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2176    2176 
   Size:       4157
 
FspI TGC'GCA

Cuts at:   1938    1938 
   Size:       4157
 
HaeII r_GCGC'y

Cuts at:    702     853    1058    1089    1591    1839    2861    3840     702 
   Size:        151     205      31     502     248    1022     979    1019

  Fragments arranged by size: 

                1022    1019     979     502     248     205     151      31
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1999    2029    2031    2061    1999 
   Size:         30       2      30    4095

  Fragments arranged by size: 

                4095      30      30       2
 
HincII GTy'rAC

Cuts at:   1196    1196 
   Size:       4157
 
KasI G'GCGC_C

Cuts at:   1085    1587    1835    1085 
   Size:        502     248    3407

  Fragments arranged by size: 

                3407     502     248
 
KpnI G_GTAC'C

Cuts at:   1177    1177 
   Size:       4157
 
MluI A'CGCG_T

Cuts at:   3590    3590 
   Size:       4157
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2828    3012    2828 
   Size:        184    3973
 
MscI TGG'CCA

Cuts at:   1918    1918 
   Size:       4157
 
MslI CAynn'nnrTG

Cuts at:   2146    2273    2146 
   Size:        127    4030
 
MspA1I CmG'CkG

Cuts at:    583     593     606     638    1003    1101    1268    1582    1942 
   Size:         10      13      32     365      98     167     314     360

Cuts at:   1942    2955    3200     583 
   Size:       1013     245    1540

  Fragments arranged by size: 

                1540    1013     365     360     314     245     167      98
                  32      13      10
 
NaeI GCC'GGC

Cuts at:    137     697    2338     137 
   Size:        560    1641    1956

  Fragments arranged by size: 

                1956    1641     560
 
NarI GG'CG_CC

Cuts at:   1086    1588    1836    1086 
   Size:        502     248    3407

  Fragments arranged by size: 

                3407     502     248
 
NgoMIV G'CCGG_C

Cuts at:    135     695    2336     135 
   Size:        560    1641    1956

  Fragments arranged by size: 

                1956    1641     560
 
NspI r_CATG'y

Cuts at:   1210    1710    2617    1210 
   Size:        500     907    2750

  Fragments arranged by size: 

                2750     907     500
 
PasI CC'CwG_GG

Cuts at:    309     893     309 
   Size:        584    3573
 
PciI A'CATG_T

Cuts at:   2613    2613 
   Size:       4157
 
PmeI GTTT'AAAC

Cuts at:   1156    1156 
   Size:       4157
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3296    3328    3296 
   Size:         32    4125
 
PpuMI rG'GwC_Cy

Cuts at:    537     654     537 
   Size:        117    4040
 
PshAI GACnn'nnGTC

Cuts at:    265     265 
   Size:       4157
 
PstI C_TGCA'G

Cuts at:   1204    1889    1204 
   Size:        685    3472
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4157
 
PvuII CAG'CTG

Cuts at:    606    1582    1942     606 
   Size:        976     360    2821

  Fragments arranged by size: 

                2821     976     360
 
RsrII CG'GwC_CG

Cuts at:   2352    2352 
   Size:       4157
 
SacI G_AGCT'C

Cuts at:    203     290     791    1171     203 
   Size:         87     501     380    3189

  Fragments arranged by size: 

                3189     501     380      87
 
SacII CC_GC'GG

Cuts at:    584     639    1004     584 
   Size:         55     365    3737

  Fragments arranged by size: 

                3737     365      55
 
SalI G'TCGA_C

Cuts at:   1194    1194 
   Size:       4157
 
SapI GCTCTTCn'nnn_

Cuts at:    871    2180    2390     871 
   Size:       1309     210    2638

  Fragments arranged by size: 

                2638    1309     210
 
SbfI CC_TGCA'GG

Cuts at:   1204    1204 
   Size:       4157
 
ScaI AGT'ACT

Cuts at:   3674    3674 
   Size:       4157
 
SfcI C'TryA_G

Cuts at:     33     504    1200    1885    2878    3069      33 
   Size:        471     696     685     993     191    1121

  Fragments arranged by size: 

                1121     993     696     685     471     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1003    1003 
   Size:       4157
 
SfoI GGC'GCC

Cuts at:   1087    1589    1837    1087 
   Size:        502     248    3407

  Fragments arranged by size: 

                3407     502     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4157
 
SmaI CCC'GGG

Cuts at:    967    1179     967 
   Size:        212    3945
 
SmlI C'TyrA_G

Cuts at:   1312    2227    2719    2981    1312 
   Size:        915     492     262    2488

  Fragments arranged by size: 

                2488     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4157
 
SphI G_CATG'C

Cuts at:   1210    1710    1210 
   Size:        500    3657
 
StuI AGG'CCT

Cuts at:    732     732 
   Size:       4157
 
StyI C'CwwG_G

Cuts at:    560    1291    1645     560 
   Size:        731     354    3072

  Fragments arranged by size: 

                3072     731     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    361     727    1740    1873    2332    2541     361 
   Size:        366    1013     133     459     209    1977

  Fragments arranged by size: 

                1977    1013     459     366     209     133
 
TatI w'GTAC_w

Cuts at:    623    3672     623 
   Size:       3049    1108
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3674    3706    3674 
   Size:         32    4125
 
Tth111I GACn'n_nGTC

Cuts at:   1954    1954 
   Size:       4157
 
XbaI T'CTAG_A

Cuts at:   1188    1188 
   Size:       4157
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    322    1652     322 
   Size:       1330    2827
 
XmaI C'CCGG_G

Cuts at:    965    1177     965 
   Size:        212    3945
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PasI     PciI     PmeI     PpiI    PpuMI
    PshAI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BfrBI     BmtI    BsaXI     BsgI
    BsiWI     BsmI    BsmBI    BsrGI    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI    PflMI
     PfoI     PmlI     PsiI   PspOMI    PspXI     PsrI    SanDI    SexAI
    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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