Restriction map for
pF1KE0779
(Circular) (Six-base) MAPSORT of: pF1KE0779.seq Check: 809 from: 1 to: 4157
pF1KE0779 4157 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1195 1195
Size: 4157
Acc65I G'GTAC_C
Cuts at: 1173 1173
Size: 4157
AclI AA'CG_TT
Cuts at: 3997 3997
Size: 4157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 679 1982 2414 3161 3429 397
Size: 282 1303 432 747 268 1125
Fragments arranged by size:
1303 1125 747 432 282 268
AfeI AGC'GCT
Cuts at: 1056 1056
Size: 4157
AflIII A'CryG_T
Cuts at: 1509 2613 3590 1509
Size: 1104 977 2076
Fragments arranged by size:
2076 1104 977
AgeI A'CCGG_T
Cuts at: 2545 2545
Size: 4157
AlwNI CAG_nnn'CTG
Cuts at: 264 1462 3029 264
Size: 1198 1567 1392
Fragments arranged by size:
1567 1392 1198
ApaLI G'TGCA_C
Cuts at: 586 2927 586
Size: 2341 1816
ApoI r'AATT_y
Cuts at: 1161 3510 3766 1161
Size: 2349 256 1552
Fragments arranged by size:
2349 1552 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4157
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
AvaI C'yCGr_G
Cuts at: 784 811 965 1177 784
Size: 27 154 212 3764
Fragments arranged by size:
3764 212 154 27
BamHI G'GATC_C
Cuts at: 1182 1182
Size: 4157
BanI G'GyrC_C
Cuts at: 1085 1173 1587 1835 1870 1085
Size: 88 414 248 35 3372
Fragments arranged by size:
3372 414 248 88 35
BanII G_rGCy'C
Cuts at: 203 290 440 524 791 1171 2201 203
Size: 87 150 84 267 380 1030 2159
Fragments arranged by size:
2159 1030 380 267 150 87 84
BbeI G_GCGC'C
Cuts at: 1089 1591 1839 1089
Size: 502 248 3407
Fragments arranged by size:
3407 502 248
BbvCI CC'TCA_GC
Cuts at: 977 977
Size: 4157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 134 168 468 502 90
Size: 34 10 34 300 34 3745
Fragments arranged by size:
3745 300 34 34 34 10
BciVI GTATCCnnnnn_n'
Cuts at: 2055 2816 4157 2055
Size: 761 1341 2055
Fragments arranged by size:
2055 1341 761
BclI T'GATC_A
Cuts at: 1677 1677
Size: 4157
BglI GCCn_nnn'nGGC
Cuts at: 607 1003 607
Size: 396 3761
BglII A'GATC_T
Cuts at: 1672 3709 1672
Size: 2037 2120
BlpI GC'TnA_GC
Cuts at: 1269 1269
Size: 4157
Bme1580I G_kGCm'C
Cuts at: 590 1782 1875 2931 590
Size: 1192 93 1056 1816
Fragments arranged by size:
1816 1192 1056 93
BmgBI CAC'GTC
Cuts at: 401 401
Size: 4157
BmrI ACTGGGnnnn_n'
Cuts at: 1369 1450 1785 3929 1369
Size: 81 335 2144 1597
Fragments arranged by size:
2144 1597 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 4157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 253 640 1113 187
Size: 66 387 473 3231
Fragments arranged by size:
3231 473 387 66
Bpu10I CC'TnA_GC
Cuts at: 977 977
Size: 4157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1333 2212 2704 3002 1333
Size: 879 492 298 2488
Fragments arranged by size:
2488 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3861 3861
Size: 4157
BsaAI yAC'GTr
Cuts at: 1510 1510
Size: 4157
BsaBI GATnn'nnATC
Cuts at: 6 1066 1671 3708 6
Size: 1060 605 2037 455
Fragments arranged by size:
2037 1060 605 455
BsaHI Gr'CG_yC
Cuts at: 842 857 952 1086 1588 1836 2538 4034 842
Size: 15 95 134 502 248 702 1496 965
Fragments arranged by size:
1496 965 702 502 248 134 95 15
BsaWI w'CCGG_w
Cuts at: 1346 1867 2545 2819 2966 3377 1346
Size: 521 678 274 147 411 2126
Fragments arranged by size:
2126 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 184 211 865 1065 1153 181
Size: 3 27 654 200 88 3185
Fragments arranged by size:
3185 654 200 88 27 3
BseYI C'CCAG_C
Cuts at: 444 602 852 1144 1582 2340 2917 3413 444
Size: 158 250 292 438 758 577 496 1188
Fragments arranged by size:
1188 758 577 496 438 292 250 158
BsiEI CG_ry'CG
Cuts at: 104 1007 1699 1745 2953 104
Size: 903 692 46 1208 1308
Fragments arranged by size:
1308 1208 903 692 46
BsiHKAI G_wGCw'C
Cuts at: 203 258 290 590 791 1079 1171 1949 2139
Size: 55 32 300 201 288 92 778 190
Cuts at: 2139 2931 203
Size: 792 1429
Fragments arranged by size:
1429 792 778 300 288 201 190 92
55 32
Bsp1286I G_dGCh'C
Cuts at: 203 258 290 440 524 590 791 1079 1171
Size: 55 32 150 84 66 201 288 92
Cuts at: 1171 1782 1875 1949 2139 2201 2931 203
Size: 611 93 74 190 62 730 1429
Fragments arranged by size:
1429 730 611 288 201 190 150 93
92 84 74 66 62 55 32
BspEI T'CCGG_A
Cuts at: 3377 3377
Size: 4157
BspHI T'CATG_A
Cuts at: 3333 3333
Size: 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1140 1207 1421 1723 2104 1140
Size: 67 214 302 381 3193
Fragments arranged by size:
3193 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 807 1129 2449 2503 3987 4152 50
Size: 757 322 1320 54 1484 165 55
Fragments arranged by size:
1484 1320 757 322 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2069 2069
Size: 4157
BsrFI r'CCGG_y
Cuts at: 135 695 899 2155 2336 2545 135
Size: 560 204 1256 181 209 1747
Fragments arranged by size:
1747 1256 560 209 204 181
BssHII G'CGCG_C
Cuts at: 227 449 794 227
Size: 222 345 3590
Fragments arranged by size:
3590 345 222
BssSI C'ACGA_G
Cuts at: 726 2428 2786 726
Size: 1702 358 2097
Fragments arranged by size:
2097 1702 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1534 1534
Size: 4157
BstBI TT'CG_AA
Cuts at: 2518 2518
Size: 4157
BstEII G'GTnAC_C
Cuts at: 342 342
Size: 4157
BstYI r'GATC_y
Cuts at: 1 1182 1649 1672 2007 2253 3265 3351 3363
Size: 1181 467 23 335 246 1012 86 12
Cuts at: 3363 3709 1
Size: 346 449
Fragments arranged by size:
1181 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 408 581 636 1001 408
Size: 173 55 365 3564
Fragments arranged by size:
3564 365 173 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2107 2288 2451 2107
Size: 181 163 3813
Fragments arranged by size:
3813 181 163
BtsI GCAGTG_nn'
Cuts at: 1127 1400 1127
Size: 273 3884
DraI TTT'AAA
Cuts at: 1156 1156
Size: 4157
DrdI GACnn_nn'nnGTC
Cuts at: 1863 2721 1863
Size: 858 3299
EaeI y'GGCC_r
Cuts at: 327 720 755 908 1004 1742 1916 2307 2334
Size: 393 35 153 96 738 174 391 27
Cuts at: 2334 3679 327
Size: 1345 805
Fragments arranged by size:
1345 805 738 393 391 174 153 96
35 27
EagI C'GGCC_G
Cuts at: 1004 1742 1004
Size: 738 3419
EarI CTCTTCn'nnn_
Cuts at: 871 1130 2180 2390 871
Size: 259 1050 210 2638
Fragments arranged by size:
2638 1050 259 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 154 603 1129 2365 2675 2821 3967 154
Size: 449 526 1236 310 146 1146 344
Fragments arranged by size:
1236 1146 526 449 344 310 146
EcoICRI GAG'CTC
Cuts at: 201 288 789 1169 201
Size: 87 501 380 3189
Fragments arranged by size:
3189 501 380 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 253 397 640 679 1113 1982 2414 3161
Size: 66 144 243 39 434 869 432 747
Cuts at: 3161 3429 187
Size: 268 915
Fragments arranged by size:
915 869 747 434 432 268 243 144
66 39
EcoNI CCTnn'n_nnAGG
Cuts at: 313 910 313
Size: 597 3560
EcoO109I rG'GnC_Cy
Cuts at: 537 654 816 1296 537
Size: 117 162 480 3398
Fragments arranged by size:
3398 480 162 117
EcoRI G'AATT_C
Cuts at: 1161 1161
Size: 4157
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 4157
FspI TGC'GCA
Cuts at: 1938 1938
Size: 4157
HaeII r_GCGC'y
Cuts at: 702 853 1058 1089 1591 1839 2861 3840 702
Size: 151 205 31 502 248 1022 979 1019
Fragments arranged by size:
1022 1019 979 502 248 205 151 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1999 2029 2031 2061 1999
Size: 30 2 30 4095
Fragments arranged by size:
4095 30 30 2
HincII GTy'rAC
Cuts at: 1196 1196
Size: 4157
KasI G'GCGC_C
Cuts at: 1085 1587 1835 1085
Size: 502 248 3407
Fragments arranged by size:
3407 502 248
KpnI G_GTAC'C
Cuts at: 1177 1177
Size: 4157
MluI A'CGCG_T
Cuts at: 3590 3590
Size: 4157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2828 3012 2828
Size: 184 3973
MscI TGG'CCA
Cuts at: 1918 1918
Size: 4157
MslI CAynn'nnrTG
Cuts at: 2146 2273 2146
Size: 127 4030
MspA1I CmG'CkG
Cuts at: 583 593 606 638 1003 1101 1268 1582 1942
Size: 10 13 32 365 98 167 314 360
Cuts at: 1942 2955 3200 583
Size: 1013 245 1540
Fragments arranged by size:
1540 1013 365 360 314 245 167 98
32 13 10
NaeI GCC'GGC
Cuts at: 137 697 2338 137
Size: 560 1641 1956
Fragments arranged by size:
1956 1641 560
NarI GG'CG_CC
Cuts at: 1086 1588 1836 1086
Size: 502 248 3407
Fragments arranged by size:
3407 502 248
NgoMIV G'CCGG_C
Cuts at: 135 695 2336 135
Size: 560 1641 1956
Fragments arranged by size:
1956 1641 560
NspI r_CATG'y
Cuts at: 1210 1710 2617 1210
Size: 500 907 2750
Fragments arranged by size:
2750 907 500
PasI CC'CwG_GG
Cuts at: 309 893 309
Size: 584 3573
PciI A'CATG_T
Cuts at: 2613 2613
Size: 4157
PmeI GTTT'AAAC
Cuts at: 1156 1156
Size: 4157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3296 3328 3296
Size: 32 4125
PpuMI rG'GwC_Cy
Cuts at: 537 654 537
Size: 117 4040
PshAI GACnn'nnGTC
Cuts at: 265 265
Size: 4157
PstI C_TGCA'G
Cuts at: 1204 1889 1204
Size: 685 3472
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4157
PvuII CAG'CTG
Cuts at: 606 1582 1942 606
Size: 976 360 2821
Fragments arranged by size:
2821 976 360
RsrII CG'GwC_CG
Cuts at: 2352 2352
Size: 4157
SacI G_AGCT'C
Cuts at: 203 290 791 1171 203
Size: 87 501 380 3189
Fragments arranged by size:
3189 501 380 87
SacII CC_GC'GG
Cuts at: 584 639 1004 584
Size: 55 365 3737
Fragments arranged by size:
3737 365 55
SalI G'TCGA_C
Cuts at: 1194 1194
Size: 4157
SapI GCTCTTCn'nnn_
Cuts at: 871 2180 2390 871
Size: 1309 210 2638
Fragments arranged by size:
2638 1309 210
SbfI CC_TGCA'GG
Cuts at: 1204 1204
Size: 4157
ScaI AGT'ACT
Cuts at: 3674 3674
Size: 4157
SfcI C'TryA_G
Cuts at: 33 504 1200 1885 2878 3069 33
Size: 471 696 685 993 191 1121
Fragments arranged by size:
1121 993 696 685 471 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1003 1003
Size: 4157
SfoI GGC'GCC
Cuts at: 1087 1589 1837 1087
Size: 502 248 3407
Fragments arranged by size:
3407 502 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
SmaI CCC'GGG
Cuts at: 967 1179 967
Size: 212 3945
SmlI C'TyrA_G
Cuts at: 1312 2227 2719 2981 1312
Size: 915 492 262 2488
Fragments arranged by size:
2488 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4157
SphI G_CATG'C
Cuts at: 1210 1710 1210
Size: 500 3657
StuI AGG'CCT
Cuts at: 732 732
Size: 4157
StyI C'CwwG_G
Cuts at: 560 1291 1645 560
Size: 731 354 3072
Fragments arranged by size:
3072 731 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 361 727 1740 1873 2332 2541 361
Size: 366 1013 133 459 209 1977
Fragments arranged by size:
1977 1013 459 366 209 133
TatI w'GTAC_w
Cuts at: 623 3672 623
Size: 3049 1108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3674 3706 3674
Size: 32 4125
Tth111I GACn'n_nGTC
Cuts at: 1954 1954
Size: 4157
XbaI T'CTAG_A
Cuts at: 1188 1188
Size: 4157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 322 1652 322
Size: 1330 2827
XmaI C'CCGG_G
Cuts at: 965 1177 965
Size: 212 3945
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BfrBI BmtI BsaXI BsgI
BsiWI BsmI BsmBI BsrGI BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PsiI PspOMI PspXI PsrI SanDI SexAI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI