Restriction map for
pF1KE0774
(Circular) (Six-base) MAPSORT of: pF1KE0774.seq Check: 5401 from: 1 to: 4448
pF1KE0774 4448 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 849 1253 1486 849
Size: 404 233 3811
Fragments arranged by size:
3811 404 233
Acc65I G'GTAC_C
Cuts at: 1464 1464
Size: 4448
AclI AA'CG_TT
Cuts at: 4288 4288
Size: 4448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1004 1031 2273 2705 3452 3720 1004
Size: 27 1242 432 747 268 1732
Fragments arranged by size:
1732 1242 747 432 268 27
AflIII A'CryG_T
Cuts at: 1800 2904 3881 1800
Size: 1104 977 2367
Fragments arranged by size:
2367 1104 977
AgeI A'CCGG_T
Cuts at: 2836 2836
Size: 4448
AlwNI CAG_nnn'CTG
Cuts at: 1129 1753 3320 1129
Size: 624 1567 2257
Fragments arranged by size:
2257 1567 624
ApaI G_GGCC'C
Cuts at: 1195 1195
Size: 4448
ApaLI G'TGCA_C
Cuts at: 3218 3218
Size: 4448
ApoI r'AATT_y
Cuts at: 640 934 1207 1452 3801 4057 640
Size: 294 273 245 2349 256 1031
Fragments arranged by size:
2349 1031 294 273 256 245
AseI AT'TA_AT
Cuts at: 20 562 770 20
Size: 542 208 3698
Fragments arranged by size:
3698 542 208
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
AvaI C'yCGr_G
Cuts at: 1468 1468
Size: 4448
BamHI G'GATC_C
Cuts at: 1473 1473
Size: 4448
BanI G'GyrC_C
Cuts at: 148 1464 1878 2126 2161 148
Size: 1316 414 248 35 2435
Fragments arranged by size:
2435 1316 414 248 35
BanII G_rGCy'C
Cuts at: 1195 1228 1462 2492 1195
Size: 33 234 1030 3151
Fragments arranged by size:
3151 1030 234 33
BbeI G_GCGC'C
Cuts at: 1882 2130 1882
Size: 248 4200
BbsI GAAGACnn'nnnn_
Cuts at: 314 314
Size: 4448
BciVI GTATCCnnnnn_n'
Cuts at: 2346 3107 4448 2346
Size: 761 1341 2346
Fragments arranged by size:
2346 1341 761
BclI T'GATC_A
Cuts at: 1218 1968 1218
Size: 750 3698
BfrBI ATG'CAT
Cuts at: 559 559
Size: 4448
BglII A'GATC_T
Cuts at: 1963 4000 1963
Size: 2037 2411
BlpI GC'TnA_GC
Cuts at: 1560 1560
Size: 4448
Bme1580I G_kGCm'C
Cuts at: 151 1195 2073 2166 3222 151
Size: 1044 878 93 1056 1377
Fragments arranged by size:
1377 1056 1044 878 93
BmrI ACTGGGnnnn_n'
Cuts at: 1660 1741 2076 4220 1660
Size: 81 335 2144 1888
Fragments arranged by size:
2144 1888 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1212 1212
Size: 4448
Bpu10I CC'TnA_GC
Cuts at: 828 828
Size: 4448
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1624 2503 2995 3293 1624
Size: 879 492 298 2779
Fragments arranged by size:
2779 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 172 4152 172
Size: 3980 468
BsaAI yAC'GTr
Cuts at: 1305 1801 1305
Size: 496 3952
BsaBI GATnn'nnATC
Cuts at: 6 1962 3999 6
Size: 1956 2037 455
Fragments arranged by size:
2037 1956 455
BsaHI Gr'CG_yC
Cuts at: 1879 2127 2829 4325 1879
Size: 248 702 1496 2002
Fragments arranged by size:
2002 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1637 2158 2836 3110 3257 3668 1637
Size: 521 678 274 147 411 2417
Fragments arranged by size:
2417 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 127 157 127
Size: 30 4418
BseYI C'CCAG_C
Cuts at: 1873 2631 3208 3704 1873
Size: 758 577 496 2617
Fragments arranged by size:
2617 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1990 2036 3244 104
Size: 1886 46 1208 1308
Fragments arranged by size:
1886 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 829 1462 2240 2430 3222 829
Size: 633 778 190 792 2055
Fragments arranged by size:
2055 792 778 633 190
Bsp1286I G_dGCh'C
Cuts at: 151 829 1195 1228 1462 2073 2166 2240 2430
Size: 678 366 33 234 611 93 74 190
Cuts at: 2430 2492 3222 151
Size: 62 730 1377
Fragments arranged by size:
1377 730 678 611 366 234 190 93
74 62 33
BspEI T'CCGG_A
Cuts at: 3668 3668
Size: 4448
BspHI T'CATG_A
Cuts at: 1145 3624 1145
Size: 2479 1969
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1498 1712 2014 2395 1498
Size: 214 302 381 3551
Fragments arranged by size:
3551 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2740 2794 4278 4443 50
Size: 2690 54 1484 165 55
Fragments arranged by size:
2690 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2360 2360
Size: 4448
BsrFI r'CCGG_y
Cuts at: 2446 2627 2836 2446
Size: 181 209 4058
Fragments arranged by size:
4058 209 181
BssSI C'ACGA_G
Cuts at: 2719 3077 2719
Size: 358 4090
BstAPI GCAn_nnn'nTGC
Cuts at: 1825 1825
Size: 4448
BstBI TT'CG_AA
Cuts at: 2809 2809
Size: 4448
BstXI CCAn_nnnn'nTGG
Cuts at: 993 1264 993
Size: 271 4177
BstYI r'GATC_y
Cuts at: 1 226 735 1125 1161 1338 1473 1940 1963
Size: 225 509 390 36 177 135 467 23
Cuts at: 1963 2298 2544 3556 3642 3654 4000 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 509 467 449 390 346 335 246
225 177 135 86 36 23 12
BstZ17I GTA'TAC
Cuts at: 850 850
Size: 4448
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2398 2579 2742 2398
Size: 181 163 4104
Fragments arranged by size:
4104 181 163
BtsI GCAGTG_nn'
Cuts at: 963 1691 963
Size: 728 3720
DraI TTT'AAA
Cuts at: 1447 1447
Size: 4448
DrdI GACnn_nn'nnGTC
Cuts at: 2154 3012 2154
Size: 858 3590
EaeI y'GGCC_r
Cuts at: 1393 2033 2207 2598 2625 3970 1393
Size: 640 174 391 27 1345 1871
Fragments arranged by size:
1871 1345 640 391 174 27
EagI C'GGCC_G
Cuts at: 2033 2033
Size: 4448
EarI CTCTTCn'nnn_
Cuts at: 341 527 1029 2471 2681 341
Size: 186 502 1442 210 2108
Fragments arranged by size:
2108 1442 502 210 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2656 2966 3112 4258 2656
Size: 310 146 1146 2846
Fragments arranged by size:
2846 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1460 1460
Size: 4448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1004 1031 1212 2273 2705 3452 3720 1004
Size: 27 181 1061 432 747 268 1732
Fragments arranged by size:
1732 1061 747 432 268 181 27
EcoO109I rG'GnC_Cy
Cuts at: 1587 1587
Size: 4448
EcoRI G'AATT_C
Cuts at: 1452 1452
Size: 4448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 4448
FspI TGC'GCA
Cuts at: 2229 2229
Size: 4448
HaeII r_GCGC'y
Cuts at: 1882 2130 3152 4131 1882
Size: 248 1022 979 2199
Fragments arranged by size:
2199 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1163 1195 1210 1242 1407 1439 2290 2320 2322
Size: 32 15 32 165 32 851 30 2
Cuts at: 2322 2352 1163
Size: 30 3259
Fragments arranged by size:
3259 851 165 32 32 32 30 30
15 2
HincII GTy'rAC
Cuts at: 1487 1487
Size: 4448
HindIII A'AGCT_T
Cuts at: 890 890
Size: 4448
KasI G'GCGC_C
Cuts at: 1878 2126 1878
Size: 248 4200
KpnI G_GTAC'C
Cuts at: 1468 1468
Size: 4448
MluI A'CGCG_T
Cuts at: 3881 3881
Size: 4448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3119 3303 3119
Size: 184 4264
MscI TGG'CCA
Cuts at: 1395 2209 1395
Size: 814 3634
MslI CAynn'nnrTG
Cuts at: 1262 2437 2564 1262
Size: 1175 127 3146
Fragments arranged by size:
3146 1175 127
MspA1I CmG'CkG
Cuts at: 191 1559 1873 2233 3246 3491 191
Size: 1368 314 360 1013 245 1148
Fragments arranged by size:
1368 1148 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2629 2629
Size: 4448
NarI GG'CG_CC
Cuts at: 1879 2127 1879
Size: 248 4200
NgoMIV G'CCGG_C
Cuts at: 2627 2627
Size: 4448
NsiI A_TGCA'T
Cuts at: 561 561
Size: 4448
NspI r_CATG'y
Cuts at: 793 1501 2001 2908 793
Size: 708 500 907 2333
Fragments arranged by size:
2333 907 708 500
PacI TTA_AT'TAA
Cuts at: 770 770
Size: 4448
PciI A'CATG_T
Cuts at: 2904 2904
Size: 4448
PmeI GTTT'AAAC
Cuts at: 1447 1447
Size: 4448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 3587
Size: 32 4416
PsiI TTA'TAA
Cuts at: 1286 1286
Size: 4448
PspOMI G'GGCC_C
Cuts at: 1191 1191
Size: 4448
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 897 929 897
Size: 32 4416
PstI C_TGCA'G
Cuts at: 489 1320 1495 2180 489
Size: 831 175 685 2757
Fragments arranged by size:
2757 831 685 175
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4448
PvuII CAG'CTG
Cuts at: 1873 2233 1873
Size: 360 4088
RsrII CG'GwC_CG
Cuts at: 2643 2643
Size: 4448
SacI G_AGCT'C
Cuts at: 1462 1462
Size: 4448
SalI G'TCGA_C
Cuts at: 1485 1485
Size: 4448
SapI GCTCTTCn'nnn_
Cuts at: 341 527 2471 2681 341
Size: 186 1944 210 2108
Fragments arranged by size:
2108 1944 210 186
SbfI CC_TGCA'GG
Cuts at: 1495 1495
Size: 4448
ScaI AGT'ACT
Cuts at: 3965 3965
Size: 4448
SfcI C'TryA_G
Cuts at: 33 485 1316 1348 1491 2176 3169 3360 33
Size: 452 831 32 143 685 993 191 1121
Fragments arranged by size:
1121 993 831 685 452 191 143 32
SfoI GGC'GCC
Cuts at: 1880 2128 1880
Size: 248 4200
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
SmaI CCC'GGG
Cuts at: 1470 1470
Size: 4448
SmlI C'TyrA_G
Cuts at: 1603 2518 3010 3272 1603
Size: 915 492 262 2779
Fragments arranged by size:
2779 915 492 262
SnaBI TAC'GTA
Cuts at: 1305 1305
Size: 4448
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4448
SphI G_CATG'C
Cuts at: 1501 2001 1501
Size: 500 3948
StyI C'CwwG_G
Cuts at: 521 986 1582 1936 521
Size: 465 596 354 3033
Fragments arranged by size:
3033 596 465 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2031 2164 2623 2832 2031
Size: 133 459 209 3647
Fragments arranged by size:
3647 459 209 133
TatI w'GTAC_w
Cuts at: 3963 3963
Size: 4448
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 127 159 3965 3997 127
Size: 32 3806 32 578
Fragments arranged by size:
3806 578 32 32
Tth111I GACn'n_nGTC
Cuts at: 2245 2245
Size: 4448
XbaI T'CTAG_A
Cuts at: 1479 1479
Size: 4448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1943 1943
Size: 4448
XmaI C'CCGG_G
Cuts at: 1468 1468
Size: 4448
XmnI GAAnn'nnTTC
Cuts at: 1211 1211
Size: 4448
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PacI PciI PmeI PpiI PsiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
PasI PflMI PfoI PmlI PpuMI PshAI PspXI SacII
SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI
XhoI ZraI