Restriction map for
pF1KE0769
(Circular) (Six-base) MAPSORT of: pF1KE0769.seq Check: 5311 from: 1 to: 4130
pF1KE0769 4130 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 269 935 269
Size: 666 3464
AccI GT'mk_AC
Cuts at: 1168 1168
Size: 4130
Acc65I G'GTAC_C
Cuts at: 1146 1146
Size: 4130
AclI AA'CG_TT
Cuts at: 3970 3970
Size: 4130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 1955 2387 3134 3402 382
Size: 1573 432 747 268 1110
Fragments arranged by size:
1573 1110 747 432 268
AflIII A'CryG_T
Cuts at: 1482 2586 3563 1482
Size: 1104 977 2049
Fragments arranged by size:
2049 1104 977
AgeI A'CCGG_T
Cuts at: 2518 2518
Size: 4130
AleI CACnn'nnGTG
Cuts at: 556 556
Size: 4130
AlwNI CAG_nnn'CTG
Cuts at: 255 767 1119 1435 3002 255
Size: 512 352 316 1567 1383
Fragments arranged by size:
1567 1383 512 352 316
ApaI G_GGCC'C
Cuts at: 344 1052 344
Size: 708 3422
ApaLI G'TGCA_C
Cuts at: 2900 2900
Size: 4130
ApoI r'AATT_y
Cuts at: 1134 3483 3739 1134
Size: 2349 256 1525
Fragments arranged by size:
2349 1525 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4130
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
AvaI C'yCGr_G
Cuts at: 594 644 680 1150 594
Size: 50 36 470 3574
Fragments arranged by size:
3574 470 50 36
BamHI G'GATC_C
Cuts at: 1155 1155
Size: 4130
BanI G'GyrC_C
Cuts at: 146 354 876 1146 1560 1808 1843 146
Size: 208 522 270 414 248 35 2433
Fragments arranged by size:
2433 522 414 270 248 208 35
BanII G_rGCy'C
Cuts at: 196 332 344 401 1052 1144 2174 196
Size: 136 12 57 651 92 1030 2152
Fragments arranged by size:
2152 1030 651 136 92 57 12
BbeI G_GCGC'C
Cuts at: 1564 1812 1564
Size: 248 3882
BbsI GAAGACnn'nnnn_
Cuts at: 882 882
Size: 4130
BbvCI CC'TCA_GC
Cuts at: 1084 1084
Size: 4130
BciVI GTATCCnnnnn_n'
Cuts at: 2028 2789 4130 2028
Size: 761 1341 2028
Fragments arranged by size:
2028 1341 761
BclI T'GATC_A
Cuts at: 411 1650 411
Size: 1239 2891
BglI GCCn_nnn'nGGC
Cuts at: 175 175
Size: 4130
BglII A'GATC_T
Cuts at: 1645 3682 1645
Size: 2037 2093
BlpI GC'TnA_GC
Cuts at: 1242 1242
Size: 4130
Bme1580I G_kGCm'C
Cuts at: 151 321 344 359 516 774 881 1052 1755
Size: 170 23 15 157 258 107 171 703
Cuts at: 1755 1848 2904 151
Size: 93 1056 1377
Fragments arranged by size:
1377 1056 703 258 171 170 157 107
93 23 15
BmgBI CAC'GTC
Cuts at: 795 795
Size: 4130
BmrI ACTGGGnnnn_n'
Cuts at: 1342 1423 1758 3902 1342
Size: 81 335 2144 1570
Fragments arranged by size:
2144 1570 335 81
BmtI G_CTAG'C
Cuts at: 836 836
Size: 4130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 4130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 744 970 744
Size: 226 3904
Bpu10I CC'TnA_GC
Cuts at: 821 1084 821
Size: 263 3867
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1306 2185 2677 2975 1306
Size: 879 492 298 2461
Fragments arranged by size:
2461 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 447 616 3834 447
Size: 169 3218 743
Fragments arranged by size:
3218 743 169
BsaAI yAC'GTr
Cuts at: 1483 1483
Size: 4130
BsaBI GATnn'nnATC
Cuts at: 6 1644 3681 6
Size: 1638 2037 455
Fragments arranged by size:
2037 1638 455
BsaHI Gr'CG_yC
Cuts at: 1561 1809 2511 4007 1561
Size: 248 702 1496 1684
Fragments arranged by size:
1684 1496 702 248
BsaWI w'CCGG_w
Cuts at: 893 1319 1840 2518 2792 2939 3350 893
Size: 426 521 678 274 147 411 1673
Fragments arranged by size:
1673 678 521 426 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 1048 325
Size: 723 3407
BseYI C'CCAG_C
Cuts at: 195 626 798 825 943 1555 2313 2890 3386
Size: 431 172 27 118 612 758 577 496
Cuts at: 3386 195
Size: 939
Fragments arranged by size:
939 758 612 577 496 431 172 118
27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 597 241
Size: 356 3774
BsiEI CG_ry'CG
Cuts at: 104 408 1672 1718 2926 104
Size: 304 1264 46 1208 1308
Fragments arranged by size:
1308 1264 1208 304 46
BsiHKAI G_wGCw'C
Cuts at: 926 1144 1922 2112 2904 926
Size: 218 778 190 792 2152
Fragments arranged by size:
2152 792 778 218 190
BsmBI CGTCTCn'nnnn_
Cuts at: 785 785
Size: 4130
Bsp1286I G_dGCh'C
Cuts at: 151 196 321 332 344 359 401 516 774
Size: 45 125 11 12 15 42 115 258
Cuts at: 774 881 926 1052 1144 1755 1848 1922 2112
Size: 107 45 126 92 611 93 74 190
Cuts at: 2112 2174 2904 151
Size: 62 730 1377
Fragments arranged by size:
1377 730 611 258 190 126 125 115
107 93 92 74 62 45 45 42
15 12 11
BspEI T'CCGG_A
Cuts at: 893 3350 893
Size: 2457 1673
BspHI T'CATG_A
Cuts at: 3306 3306
Size: 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 269 935 1180 1394 1696 2077 269
Size: 666 245 214 302 381 2322
Fragments arranged by size:
2322 666 381 302 245 214
BsrBI CCG'CTC
Cuts at: 50 275 315 593 2422 2476 3960 4125 50
Size: 225 40 278 1829 54 1484 165 55
Fragments arranged by size:
1829 1484 278 225 165 55 54 40
BsrDI GCAATG_nn'
Cuts at: 2042 2042
Size: 4130
BsrFI r'CCGG_y
Cuts at: 930 2128 2309 2518 930
Size: 1198 181 209 2542
Fragments arranged by size:
2542 1198 209 181
BssSI C'ACGA_G
Cuts at: 699 2401 2759 699
Size: 1702 358 2070
Fragments arranged by size:
2070 1702 358
BstAPI GCAn_nnn'nTGC
Cuts at: 264 866 1507 264
Size: 602 641 2887
Fragments arranged by size:
2887 641 602
BstBI TT'CG_AA
Cuts at: 2491 2491
Size: 4130
BstYI r'GATC_y
Cuts at: 1 251 1155 1622 1645 1980 2226 3238 3324
Size: 250 904 467 23 335 246 1012 86
Cuts at: 3324 3336 3682 1
Size: 12 346 449
Fragments arranged by size:
1012 904 467 449 346 335 250 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 121 308 121
Size: 187 3943
BtgI C'CryG_G
Cuts at: 107 187 606 107
Size: 80 419 3631
Fragments arranged by size:
3631 419 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2080 2261 2424 2080
Size: 181 163 3786
Fragments arranged by size:
3786 181 163
BtsI GCAGTG_nn'
Cuts at: 486 871 1373 486
Size: 385 502 3243
Fragments arranged by size:
3243 502 385
DraI TTT'AAA
Cuts at: 1129 1129
Size: 4130
DraIII CAC_nnn'GTG
Cuts at: 146 866 146
Size: 720 3410
DrdI GACnn_nn'nnGTC
Cuts at: 1836 2694 1836
Size: 858 3272
EaeI y'GGCC_r
Cuts at: 405 693 837 1715 1889 2280 2307 3652 405
Size: 288 144 878 174 391 27 1345 883
Fragments arranged by size:
1345 883 878 391 288 174 144 27
EagI C'GGCC_G
Cuts at: 405 1715 405
Size: 1310 2820
EarI CTCTTCn'nnn_
Cuts at: 214 997 2153 2363 214
Size: 783 1156 210 1981
Fragments arranged by size:
1981 1156 783 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2338 2648 2794 3940 2338
Size: 310 146 1146 2528
Fragments arranged by size:
2528 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1142 1142
Size: 4130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 744 970 1955 2387 3134 3402 382
Size: 362 226 985 432 747 268 1110
Fragments arranged by size:
1110 985 747 432 362 268 226
EcoO109I rG'GnC_Cy
Cuts at: 340 384 599 685 764 803 1049 1116 1269
Size: 44 215 86 79 39 246 67 153
Cuts at: 1269 340
Size: 3201
Fragments arranged by size:
3201 246 215 153 86 79 67 44
39
EcoRI G'AATT_C
Cuts at: 1134 1134
Size: 4130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 4130
FspI TGC'GCA
Cuts at: 562 859 1911 562
Size: 297 1052 2781
Fragments arranged by size:
2781 1052 297
FspAI rTGC'GCAy
Cuts at: 859 859
Size: 4130
HaeII r_GCGC'y
Cuts at: 1564 1812 2834 3813 1564
Size: 248 1022 979 1881
Fragments arranged by size:
1881 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 403 435 1972 2002 2004 2034 403
Size: 32 1537 30 2 30 2499
Fragments arranged by size:
2499 1537 32 30 30 2
HincII GTy'rAC
Cuts at: 1169 1169
Size: 4130
KasI G'GCGC_C
Cuts at: 1560 1808 1560
Size: 248 3882
KpnI G_GTAC'C
Cuts at: 1150 1150
Size: 4130
MluI A'CGCG_T
Cuts at: 3563 3563
Size: 4130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 482 2801 2985 482
Size: 2319 184 1627
Fragments arranged by size:
2319 1627 184
MscI TGG'CCA
Cuts at: 695 1891 695
Size: 1196 2934
MslI CAynn'nnrTG
Cuts at: 556 2119 2246 556
Size: 1563 127 2440
Fragments arranged by size:
2440 1563 127
MspA1I CmG'CkG
Cuts at: 285 608 630 636 1241 1555 1915 2928 3173
Size: 323 22 6 605 314 360 1013 245
Cuts at: 3173 285
Size: 1242
Fragments arranged by size:
1242 1013 605 360 323 314 245 22
6
NaeI GCC'GGC
Cuts at: 932 2311 932
Size: 1379 2751
NarI GG'CG_CC
Cuts at: 1561 1809 1561
Size: 248 3882
NcoI C'CATG_G
Cuts at: 107 107
Size: 4130
NgoMIV G'CCGG_C
Cuts at: 930 2309 930
Size: 1379 2751
NheI G'CTAG_C
Cuts at: 832 832
Size: 4130
NspI r_CATG'y
Cuts at: 1183 1683 2590 1183
Size: 500 907 2723
Fragments arranged by size:
2723 907 500
PasI CC'CwG_GG
Cuts at: 337 337
Size: 4130
PciI A'CATG_T
Cuts at: 2586 2586
Size: 4130
PflMI CCAn_nnn'nTGG
Cuts at: 438 438
Size: 4130
PmeI GTTT'AAAC
Cuts at: 1129 1129
Size: 4130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 974 1006 3269 3301 974
Size: 32 2263 32 1803
Fragments arranged by size:
2263 1803 32 32
PpuMI rG'GwC_Cy
Cuts at: 685 685
Size: 4130
PspOMI G'GGCC_C
Cuts at: 340 1048 340
Size: 708 3422
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1012 1044 1012
Size: 32 4098
PstI C_TGCA'G
Cuts at: 635 1115 1177 1862 635
Size: 480 62 685 2903
Fragments arranged by size:
2903 685 480 62
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4130
PvuII CAG'CTG
Cuts at: 285 630 636 1555 1915 285
Size: 345 6 919 360 2500
Fragments arranged by size:
2500 919 360 345 6
RsrII CG'GwC_CG
Cuts at: 2325 2325
Size: 4130
SacI G_AGCT'C
Cuts at: 1144 1144
Size: 4130
SacII CC_GC'GG
Cuts at: 609 609
Size: 4130
SalI G'TCGA_C
Cuts at: 1167 1167
Size: 4130
SapI GCTCTTCn'nnn_
Cuts at: 214 2153 2363 214
Size: 1939 210 1981
Fragments arranged by size:
1981 1939 210
SbfI CC_TGCA'GG
Cuts at: 1177 1177
Size: 4130
ScaI AGT'ACT
Cuts at: 3647 3647
Size: 4130
SexAI A'CCwGG_T
Cuts at: 142 884 142
Size: 742 3388
SfcI C'TryA_G
Cuts at: 33 631 1111 1173 1858 2851 3042 33
Size: 598 480 62 685 993 191 1121
Fragments arranged by size:
1121 993 685 598 480 191 62
SfoI GGC'GCC
Cuts at: 1562 1810 1562
Size: 248 3882
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
SmaI CCC'GGG
Cuts at: 682 1152 682
Size: 470 3660
SmlI C'TyrA_G
Cuts at: 1285 2200 2692 2954 1285
Size: 915 492 262 2461
Fragments arranged by size:
2461 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4130
SphI G_CATG'C
Cuts at: 1183 1683 1183
Size: 500 3630
StuI AGG'CCT
Cuts at: 652 652
Size: 4130
StyI C'CwwG_G
Cuts at: 107 1264 1618 107
Size: 1157 354 2619
Fragments arranged by size:
2619 1157 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 817 1713 1846 2305 2514 817
Size: 896 133 459 209 2433
Fragments arranged by size:
2433 896 459 209 133
TatI w'GTAC_w
Cuts at: 3645 3645
Size: 4130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3647 3679 3647
Size: 32 4098
Tth111I GACn'n_nGTC
Cuts at: 1927 1927
Size: 4130
XbaI T'CTAG_A
Cuts at: 1161 1161
Size: 4130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1625 1625
Size: 4130
XmaI C'CCGG_G
Cuts at: 680 1150 680
Size: 470 3660
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PmeI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BsaXI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI EcoNI EcoRV FseI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PspXI SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI