Restriction map for
pF1KE0763
(Circular) (Six-base) MAPSORT of: pF1KE0763.seq Check: 3612 from: 1 to: 4124
pF1KE0763 4124 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1162 1162
Size: 4124
Acc65I G'GTAC_C
Cuts at: 1140 1140
Size: 4124
AclI AA'CG_TT
Cuts at: 1118 3964 1118
Size: 2846 1278
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1949 2381 3128 3396 1949
Size: 432 747 268 2677
Fragments arranged by size:
2677 747 432 268
AflIII A'CryG_T
Cuts at: 929 1476 2580 3557 929
Size: 547 1104 977 1496
Fragments arranged by size:
1496 1104 977 547
AgeI A'CCGG_T
Cuts at: 2512 2512
Size: 4124
AlwNI CAG_nnn'CTG
Cuts at: 279 1429 2996 279
Size: 1150 1567 1407
Fragments arranged by size:
1567 1407 1150
ApaLI G'TGCA_C
Cuts at: 2894 2894
Size: 4124
ApoI r'AATT_y
Cuts at: 498 871 1128 3477 3733 498
Size: 373 257 2349 256 889
Fragments arranged by size:
2349 889 373 257 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4124
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
AvaI C'yCGr_G
Cuts at: 1144 1144
Size: 4124
BamHI G'GATC_C
Cuts at: 1149 1149
Size: 4124
BanI G'GyrC_C
Cuts at: 1140 1554 1802 1837 1140
Size: 414 248 35 3427
Fragments arranged by size:
3427 414 248 35
BanII G_rGCy'C
Cuts at: 1138 2168 1138
Size: 1030 3094
BbeI G_GCGC'C
Cuts at: 1558 1806 1558
Size: 248 3876
BbsI GAAGACnn'nnnn_
Cuts at: 407 407
Size: 4124
BciVI GTATCCnnnnn_n'
Cuts at: 2022 2783 4124 2022
Size: 761 1341 2022
Fragments arranged by size:
2022 1341 761
BclI T'GATC_A
Cuts at: 467 1644 467
Size: 1177 2947
BglII A'GATC_T
Cuts at: 1639 3676 1639
Size: 2037 2087
BlpI GC'TnA_GC
Cuts at: 1236 1236
Size: 4124
Bme1580I G_kGCm'C
Cuts at: 1749 1842 2898 1749
Size: 93 1056 2975
Fragments arranged by size:
2975 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 267 554 1336 1417 1752 3896 267
Size: 287 782 81 335 2144 495
Fragments arranged by size:
2144 782 495 335 287 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 4124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1300 2179 2671 2969 1300
Size: 879 492 298 2455
Fragments arranged by size:
2455 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3828 3828
Size: 4124
BsaAI yAC'GTr
Cuts at: 486 1477 486
Size: 991 3133
BsaBI GATnn'nnATC
Cuts at: 6 1638 3675 6
Size: 1632 2037 455
Fragments arranged by size:
2037 1632 455
BsaHI Gr'CG_yC
Cuts at: 549 1555 1803 2505 4001 549
Size: 1006 248 702 1496 672
Fragments arranged by size:
1496 1006 702 672 248
BsaWI w'CCGG_w
Cuts at: 1313 1834 2512 2786 2933 3344 1313
Size: 521 678 274 147 411 2093
Fragments arranged by size:
2093 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 109 139 109
Size: 30 4094
BseYI C'CCAG_C
Cuts at: 391 1549 2307 2884 3380 391
Size: 1158 758 577 496 1135
Fragments arranged by size:
1158 1135 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1666 1712 2920 104
Size: 1562 46 1208 1308
Fragments arranged by size:
1562 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1138 1916 2106 2898 1138
Size: 778 190 792 2364
Fragments arranged by size:
2364 792 778 190
BsmI GAATG_Cn'
Cuts at: 953 953
Size: 4124
BsmBI CGTCTCn'nnnn_
Cuts at: 188 188
Size: 4124
Bsp1286I G_dGCh'C
Cuts at: 1138 1749 1842 1916 2106 2168 2898 1138
Size: 611 93 74 190 62 730 2364
Fragments arranged by size:
2364 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3344 3344
Size: 4124
BspHI T'CATG_A
Cuts at: 3300 3300
Size: 4124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1174 1388 1690 2071 1174
Size: 214 302 381 3227
Fragments arranged by size:
3227 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2416 2470 3954 4119 50
Size: 2366 54 1484 165 55
Fragments arranged by size:
2366 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2036 2036
Size: 4124
BsrFI r'CCGG_y
Cuts at: 2122 2303 2512 2122
Size: 181 209 3734
Fragments arranged by size:
3734 209 181
BssSI C'ACGA_G
Cuts at: 2395 2753 2395
Size: 358 3766
BstAPI GCAn_nnn'nTGC
Cuts at: 1501 1501
Size: 4124
BstBI TT'CG_AA
Cuts at: 2485 2485
Size: 4124
BstYI r'GATC_y
Cuts at: 1 1149 1616 1639 1974 2220 3232 3318 3330
Size: 1148 467 23 335 246 1012 86 12
Cuts at: 3330 3676 1
Size: 346 449
Fragments arranged by size:
1148 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 726 726
Size: 4124
BtgI C'CryG_G
Cuts at: 766 766
Size: 4124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2074 2255 2418 2074
Size: 181 163 3780
Fragments arranged by size:
3780 181 163
BtsI GCAGTG_nn'
Cuts at: 245 1367 245
Size: 1122 3002
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 822 857 822
Size: 35 4089
DraI TTT'AAA
Cuts at: 1123 1123
Size: 4124
DrdI GACnn_nn'nnGTC
Cuts at: 1830 2688 1830
Size: 858 3266
EaeI y'GGCC_r
Cuts at: 587 1709 1883 2274 2301 3646 587
Size: 1122 174 391 27 1345 1065
Fragments arranged by size:
1345 1122 1065 391 174 27
EagI C'GGCC_G
Cuts at: 1709 1709
Size: 4124
EarI CTCTTCn'nnn_
Cuts at: 1044 2147 2357 1044
Size: 1103 210 2811
Fragments arranged by size:
2811 1103 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2332 2642 2788 3934 2332
Size: 310 146 1146 2522
Fragments arranged by size:
2522 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1136 1136
Size: 4124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1949 2381 3128 3396 1949
Size: 432 747 268 2677
Fragments arranged by size:
2677 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 388 965 1263 388
Size: 577 298 3249
Fragments arranged by size:
3249 577 298
EcoRI G'AATT_C
Cuts at: 871 1128 871
Size: 257 3867
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 4124
FspI TGC'GCA
Cuts at: 1905 1905
Size: 4124
HaeII r_GCGC'y
Cuts at: 1558 1806 2828 3807 1558
Size: 248 1022 979 1875
Fragments arranged by size:
1875 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 380 412 706 738 1966 1996 1998 2028 380
Size: 32 294 32 1228 30 2 30 2476
Fragments arranged by size:
2476 1228 294 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1163 1163
Size: 4124
KasI G'GCGC_C
Cuts at: 1554 1802 1554
Size: 248 3876
KpnI G_GTAC'C
Cuts at: 1144 1144
Size: 4124
MluI A'CGCG_T
Cuts at: 3557 3557
Size: 4124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2795 2979 2795
Size: 184 3940
MscI TGG'CCA
Cuts at: 589 1885 589
Size: 1296 2828
MslI CAynn'nnrTG
Cuts at: 341 980 2113 2240 341
Size: 639 1133 127 2225
Fragments arranged by size:
2225 1133 639 127
MspA1I CmG'CkG
Cuts at: 122 1235 1549 1909 2922 3167 122
Size: 1113 314 360 1013 245 1079
Fragments arranged by size:
1113 1079 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2305 2305
Size: 4124
NarI GG'CG_CC
Cuts at: 1555 1803 1555
Size: 248 3876
NgoMIV G'CCGG_C
Cuts at: 2303 2303
Size: 4124
NspI r_CATG'y
Cuts at: 336 933 1177 1677 2584 336
Size: 597 244 500 907 1876
Fragments arranged by size:
1876 907 597 500 244
PciI A'CATG_T
Cuts at: 929 2580 929
Size: 1651 2473
PmeI GTTT'AAAC
Cuts at: 1123 1123
Size: 4124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 4092
PpuMI rG'GwC_Cy
Cuts at: 388 965 388
Size: 577 3547
PstI C_TGCA'G
Cuts at: 870 1171 1856 870
Size: 301 685 3138
Fragments arranged by size:
3138 685 301
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4124
PvuII CAG'CTG
Cuts at: 1549 1909 1549
Size: 360 3764
RsrII CG'GwC_CG
Cuts at: 2319 2319
Size: 4124
SacI G_AGCT'C
Cuts at: 1138 1138
Size: 4124
SalI G'TCGA_C
Cuts at: 1161 1161
Size: 4124
SanDI GG'GwC_CC
Cuts at: 388 388
Size: 4124
SapI GCTCTTCn'nnn_
Cuts at: 2147 2357 2147
Size: 210 3914
SbfI CC_TGCA'GG
Cuts at: 1171 1171
Size: 4124
ScaI AGT'ACT
Cuts at: 3641 3641
Size: 4124
SfcI C'TryA_G
Cuts at: 33 866 1167 1852 2845 3036 33
Size: 833 301 685 993 191 1121
Fragments arranged by size:
1121 993 833 685 301 191
SfoI GGC'GCC
Cuts at: 1556 1804 1556
Size: 248 3876
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
SmaI CCC'GGG
Cuts at: 1146 1146
Size: 4124
SmlI C'TyrA_G
Cuts at: 1279 2194 2686 2948 1279
Size: 915 492 262 2455
Fragments arranged by size:
2455 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4124
SphI G_CATG'C
Cuts at: 1177 1677 1177
Size: 500 3624
StyI C'CwwG_G
Cuts at: 1258 1612 1258
Size: 354 3770
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1707 1840 2299 2508 1707
Size: 133 459 209 3323
Fragments arranged by size:
3323 459 209 133
TatI w'GTAC_w
Cuts at: 444 596 881 3639 444
Size: 152 285 2758 929
Fragments arranged by size:
2758 929 285 152
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3641 3673 3641
Size: 32 4092
Tth111I GACn'n_nGTC
Cuts at: 1921 1921
Size: 4124
XbaI T'CTAG_A
Cuts at: 1155 1155
Size: 4124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1619 1619
Size: 4124
XmaI C'CCGG_G
Cuts at: 1144 1144
Size: 4124
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BseRI BsgI BsiWI BsrGI
BssHII BstEII BstXI BstZ17I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI