Restriction map for
pF1KE0762
(Circular) (Six-base) MAPSORT of: pF1KE0762.seq Check: 3534 from: 1 to: 4421
pF1KE0762 4421 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1373 1459 1373
Size: 86 4335
Acc65I G'GTAC_C
Cuts at: 1437 1437
Size: 4421
AclI AA'CG_TT
Cuts at: 4261 4261
Size: 4421
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 2246 2678 3425 3693 1009
Size: 1237 432 747 268 1737
Fragments arranged by size:
1737 1237 747 432 268
AflIII A'CryG_T
Cuts at: 269 986 1773 2877 3854 269
Size: 717 787 1104 977 836
Fragments arranged by size:
1104 977 836 787 717
AgeI A'CCGG_T
Cuts at: 2809 2809
Size: 4421
AlwNI CAG_nnn'CTG
Cuts at: 1726 3293 1726
Size: 1567 2854
ApaLI G'TGCA_C
Cuts at: 3191 3191
Size: 4421
ApoI r'AATT_y
Cuts at: 332 1399 1425 3774 4030 332
Size: 1067 26 2349 256 723
Fragments arranged by size:
2349 1067 723 256 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 4421
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
AvaI C'yCGr_G
Cuts at: 464 958 1441 464
Size: 494 483 3444
Fragments arranged by size:
3444 494 483
BamHI G'GATC_C
Cuts at: 1125 1446 1125
Size: 321 4100
BanI G'GyrC_C
Cuts at: 907 1089 1437 1851 2099 2134 907
Size: 182 348 414 248 35 3194
Fragments arranged by size:
3194 414 348 248 182 35
BanII G_rGCy'C
Cuts at: 581 998 1435 2465 581
Size: 417 437 1030 2537
Fragments arranged by size:
2537 1030 437 417
BbeI G_GCGC'C
Cuts at: 1855 2103 1855
Size: 248 4173
BciVI GTATCCnnnnn_n'
Cuts at: 2319 3080 4421 2319
Size: 761 1341 2319
Fragments arranged by size:
2319 1341 761
BclI T'GATC_A
Cuts at: 1941 1941
Size: 4421
BfrBI ATG'CAT
Cuts at: 669 669
Size: 4421
BglII A'GATC_T
Cuts at: 693 1936 3973 693
Size: 1243 2037 1141
Fragments arranged by size:
2037 1243 1141
BlpI GC'TnA_GC
Cuts at: 1533 1533
Size: 4421
Bme1580I G_kGCm'C
Cuts at: 967 2046 2139 3195 967
Size: 1079 93 1056 2193
Fragments arranged by size:
2193 1079 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1312 1633 1714 2049 4193 1312
Size: 321 81 335 2144 1540
Fragments arranged by size:
2144 1540 335 321 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1259 2788 1259
Size: 1529 2892
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 459
Size: 4421
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 1597 2476 2968 3266 1141
Size: 456 879 492 298 2296
Fragments arranged by size:
2296 879 492 456 298
BsaI GGTCTCn'nnnn_
Cuts at: 523 809 1343 4125 523
Size: 286 534 2782 819
Fragments arranged by size:
2782 819 534 286
BsaAI yAC'GTr
Cuts at: 861 987 1774 861
Size: 126 787 3508
Fragments arranged by size:
3508 787 126
BsaBI GATnn'nnATC
Cuts at: 6 1935 3972 6
Size: 1929 2037 455
Fragments arranged by size:
2037 1929 455
BsaHI Gr'CG_yC
Cuts at: 244 1852 2100 2802 4298 244
Size: 1608 248 702 1496 367
Fragments arranged by size:
1608 1496 702 367 248
BsaWI w'CCGG_w
Cuts at: 1610 2131 2809 3083 3230 3641 1610
Size: 521 678 274 147 411 2390
Fragments arranged by size:
2390 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 797 1031 235
Size: 562 234 3625
Fragments arranged by size:
3625 562 234
BseYI C'CCAG_C
Cuts at: 306 1846 2604 3181 3677 306
Size: 1540 758 577 496 1050
Fragments arranged by size:
1540 1050 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 529 475
Size: 54 4367
BsiEI CG_ry'CG
Cuts at: 104 1963 2009 3217 104
Size: 1859 46 1208 1308
Fragments arranged by size:
1859 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 581 845 1435 2213 2403 3195 581
Size: 264 590 778 190 792 1807
Fragments arranged by size:
1807 792 778 590 264 190
Bsp1286I G_dGCh'C
Cuts at: 581 845 967 998 1435 2046 2139 2213 2403
Size: 264 122 31 437 611 93 74 190
Cuts at: 2403 2465 3195 581
Size: 62 730 1807
Fragments arranged by size:
1807 730 611 437 264 190 122 93
74 62 31
BspEI T'CCGG_A
Cuts at: 3641 3641
Size: 4421
BspHI T'CATG_A
Cuts at: 1064 1322 3597 1064
Size: 258 2275 1888
Fragments arranged by size:
2275 1888 258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1471 1685 1987 2368 1471
Size: 214 302 381 3524
Fragments arranged by size:
3524 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2713 2767 4251 4416 50
Size: 2663 54 1484 165 55
Fragments arranged by size:
2663 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2333 2333
Size: 4421
BsrFI r'CCGG_y
Cuts at: 2419 2600 2809 2419
Size: 181 209 4031
Fragments arranged by size:
4031 209 181
BssSI C'ACGA_G
Cuts at: 2692 3050 2692
Size: 358 4063
BstAPI GCAn_nnn'nTGC
Cuts at: 1089 1798 1089
Size: 709 3712
BstBI TT'CG_AA
Cuts at: 1221 2782 1221
Size: 1561 2860
BstYI r'GATC_y
Cuts at: 1 416 693 1031 1125 1446 1913 1936 2271
Size: 415 277 338 94 321 467 23 335
Cuts at: 2271 2517 3529 3615 3627 3973 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 415 346 338 335 321
277 246 94 86 23 12
BtgI C'CryG_G
Cuts at: 932 1037 932
Size: 105 4316
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2371 2552 2715 2371
Size: 181 163 4077
Fragments arranged by size:
4077 181 163
BtsI GCAGTG_nn'
Cuts at: 122 1664 122
Size: 1542 2879
DraI TTT'AAA
Cuts at: 1420 1420
Size: 4421
DraIII CAC_nnn'GTG
Cuts at: 552 552
Size: 4421
DrdI GACnn_nn'nnGTC
Cuts at: 2127 2985 2127
Size: 858 3563
EaeI y'GGCC_r
Cuts at: 407 935 2006 2180 2571 2598 3943 407
Size: 528 1071 174 391 27 1345 885
Fragments arranged by size:
1345 1071 885 528 391 174 27
EagI C'GGCC_G
Cuts at: 2006 2006
Size: 4421
EarI CTCTTCn'nnn_
Cuts at: 586 835 1205 2444 2654 586
Size: 249 370 1239 210 2353
Fragments arranged by size:
2353 1239 370 249 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 499 2629 2939 3085 4231 499
Size: 2130 310 146 1146 689
Fragments arranged by size:
2130 1146 689 310 146
EcoICRI GAG'CTC
Cuts at: 579 1433 579
Size: 854 3567
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 1009 2246 2678 3425 3693 459
Size: 550 1237 432 747 268 1187
Fragments arranged by size:
1237 1187 747 550 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1364 1560 1364
Size: 196 4225
EcoRI G'AATT_C
Cuts at: 332 1425 332
Size: 1093 3328
EcoRV GAT'ATC
Cuts at: 867 867
Size: 4421
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 391 564 651 2440 391
Size: 173 87 1789 2372
Fragments arranged by size:
2372 1789 173 87
FspI TGC'GCA
Cuts at: 2202 2202
Size: 4421
HaeII r_GCGC'y
Cuts at: 1855 2103 3125 4104 1855
Size: 248 1022 979 2172
Fragments arranged by size:
2172 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 329 361 520 552 2263 2293 2295 2325 329
Size: 32 159 32 1711 30 2 30 2425
Fragments arranged by size:
2425 1711 159 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1460 1460
Size: 4421
HindIII A'AGCT_T
Cuts at: 373 373
Size: 4421
KasI G'GCGC_C
Cuts at: 1851 2099 1851
Size: 248 4173
KpnI G_GTAC'C
Cuts at: 1441 1441
Size: 4421
MluI A'CGCG_T
Cuts at: 3854 3854
Size: 4421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1121 1143 3092 3276 1121
Size: 22 1949 184 2266
Fragments arranged by size:
2266 1949 184 22
MscI TGG'CCA
Cuts at: 409 937 2182 409
Size: 528 1245 2648
Fragments arranged by size:
2648 1245 528
MslI CAynn'nnrTG
Cuts at: 668 859 2410 2537 668
Size: 191 1551 127 2552
Fragments arranged by size:
2552 1551 191 127
MspA1I CmG'CkG
Cuts at: 240 1532 1846 2206 3219 3464 240
Size: 1292 314 360 1013 245 1197
Fragments arranged by size:
1292 1197 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2602 2602
Size: 4421
NarI GG'CG_CC
Cuts at: 1852 2100 1852
Size: 248 4173
NgoMIV G'CCGG_C
Cuts at: 2600 2600
Size: 4421
NsiI A_TGCA'T
Cuts at: 671 671
Size: 4421
NspI r_CATG'y
Cuts at: 273 669 1474 1974 2881 273
Size: 396 805 500 907 1813
Fragments arranged by size:
1813 907 805 500 396
PciI A'CATG_T
Cuts at: 269 2877 269
Size: 2608 1813
PfoI T'CCnGG_A
Cuts at: 419 833 419
Size: 414 4007
PmeI GTTT'AAAC
Cuts at: 1420 1420
Size: 4421
PmlI CAC'GTG
Cuts at: 987 987
Size: 4421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3560 3592 3560
Size: 32 4389
PpuMI rG'GwC_Cy
Cuts at: 1364 1364
Size: 4421
PspXI vC'TCGA_Gb
Cuts at: 958 958
Size: 4421
PstI C_TGCA'G
Cuts at: 117 1468 2153 117
Size: 1351 685 2385
Fragments arranged by size:
2385 1351 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4421
PvuII CAG'CTG
Cuts at: 240 1846 2206 240
Size: 1606 360 2455
Fragments arranged by size:
2455 1606 360
RsrII CG'GwC_CG
Cuts at: 2616 2616
Size: 4421
SacI G_AGCT'C
Cuts at: 581 1435 581
Size: 854 3567
SalI G'TCGA_C
Cuts at: 1458 1458
Size: 4421
SanDI GG'GwC_CC
Cuts at: 1364 1364
Size: 4421
SapI GCTCTTCn'nnn_
Cuts at: 586 1205 2444 2654 586
Size: 619 1239 210 2353
Fragments arranged by size:
2353 1239 619 210
SbfI CC_TGCA'GG
Cuts at: 1468 1468
Size: 4421
ScaI AGT'ACT
Cuts at: 3938 3938
Size: 4421
SfcI C'TryA_G
Cuts at: 33 113 1266 1374 1464 2149 3142 3333 33
Size: 80 1153 108 90 685 993 191 1121
Fragments arranged by size:
1153 1121 993 685 191 108 90 80
SfoI GGC'GCC
Cuts at: 1853 2101 1853
Size: 248 4173
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
SmaI CCC'GGG
Cuts at: 1443 1443
Size: 4421
SmlI C'TyrA_G
Cuts at: 958 1156 1576 2491 2983 3245 958
Size: 198 420 915 492 262 2134
Fragments arranged by size:
2134 915 492 420 262 198
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4421
SphI G_CATG'C
Cuts at: 1474 1974 1474
Size: 500 3921
StyI C'CwwG_G
Cuts at: 875 1230 1555 1909 875
Size: 355 325 354 3387
Fragments arranged by size:
3387 355 354 325
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 214 2004 2137 2596 2805 214
Size: 1790 133 459 209 1830
Fragments arranged by size:
1830 1790 459 209 133
TatI w'GTAC_w
Cuts at: 890 3936 890
Size: 3046 1375
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3938 3970 3938
Size: 32 4389
Tth111I GACn'n_nGTC
Cuts at: 2218 2218
Size: 4421
XbaI T'CTAG_A
Cuts at: 1452 1452
Size: 4421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 373 402 417 546 1916 373
Size: 29 15 129 1370 2878
Fragments arranged by size:
2878 1370 129 29 15
XhoI C'TCGA_G
Cuts at: 958 958
Size: 4421
XmaI C'CCGG_G
Cuts at: 1441 1441
Size: 4421
XmnI GAAnn'nnTTC
Cuts at: 793 793
Size: 4421
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PfoI PmeI PmlI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI PacI PasI PflMI PshAI PsiI PspOMI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI ZraI