Restriction map for
pF1KE0760
(Circular) (Six-base) MAPSORT of: pF1KE0760.seq Check: 519 from: 1 to: 4409
pF1KE0760 4409 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 511 1447 511
Size: 936 3473
Acc65I G'GTAC_C
Cuts at: 1425 1425
Size: 4409
AclI AA'CG_TT
Cuts at: 4249 4249
Size: 4409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 2666 3413 3681 2234
Size: 432 747 268 2962
Fragments arranged by size:
2962 747 432 268
AflIII A'CryG_T
Cuts at: 506 1761 2865 3842 506
Size: 1255 1104 977 1073
Fragments arranged by size:
1255 1104 1073 977
AgeI A'CCGG_T
Cuts at: 2797 2797
Size: 4409
AleI CACnn'nnGTG
Cuts at: 812 896 1316 812
Size: 84 420 3905
Fragments arranged by size:
3905 420 84
AlwNI CAG_nnn'CTG
Cuts at: 1714 3281 1714
Size: 1567 2842
ApaLI G'TGCA_C
Cuts at: 3179 3179
Size: 4409
ApoI r'AATT_y
Cuts at: 279 306 635 887 971 1139 1307 1391 1413
Size: 27 329 252 84 168 168 84 22
Cuts at: 1413 3762 4018 279
Size: 2349 256 670
Fragments arranged by size:
2349 670 329 256 252 168 168 84
84 27 22
AseI AT'TA_AT
Cuts at: 20 20
Size: 4409
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
AvaI C'yCGr_G
Cuts at: 1429 1429
Size: 4409
BamHI G'GATC_C
Cuts at: 1434 1434
Size: 4409
BanI G'GyrC_C
Cuts at: 1425 1839 2087 2122 1425
Size: 414 248 35 3712
Fragments arranged by size:
3712 414 248 35
BanII G_rGCy'C
Cuts at: 386 1201 1423 2453 386
Size: 815 222 1030 2342
Fragments arranged by size:
2342 1030 815 222
BbeI G_GCGC'C
Cuts at: 1843 2091 1843
Size: 248 4161
BbsI GAAGACnn'nnnn_
Cuts at: 509 509
Size: 4409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4375
BciVI GTATCCnnnnn_n'
Cuts at: 207 2307 3068 4409 207
Size: 2100 761 1341 207
Fragments arranged by size:
2100 1341 761 207
BclI T'GATC_A
Cuts at: 270 876 1929 270
Size: 606 1053 2750
Fragments arranged by size:
2750 1053 606
BfrBI ATG'CAT
Cuts at: 174 1059 174
Size: 885 3524
BglII A'GATC_T
Cuts at: 426 1924 3961 426
Size: 1498 2037 874
Fragments arranged by size:
2037 1498 874
BlpI GC'TnA_GC
Cuts at: 1521 1521
Size: 4409
Bme1580I G_kGCm'C
Cuts at: 2034 2127 3183 2034
Size: 93 1056 3260
Fragments arranged by size:
3260 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1621 1702 2037 4181 1621
Size: 81 335 2144 1849
Fragments arranged by size:
2144 1849 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2776 2776
Size: 4409
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 1017 1585 2464 2956 3254 227
Size: 790 568 879 492 298 1382
Fragments arranged by size:
1382 879 790 568 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 439 4113 439
Size: 3674 735
BsaAI yAC'GTr
Cuts at: 1762 1762
Size: 4409
BsaBI GATnn'nnATC
Cuts at: 6 1923 3960 6
Size: 1917 2037 455
Fragments arranged by size:
2037 1917 455
BsaHI Gr'CG_yC
Cuts at: 1840 2088 2790 4286 1840
Size: 248 702 1496 1963
Fragments arranged by size:
1963 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1598 2119 2797 3071 3218 3629 1598
Size: 521 678 274 147 411 2378
Fragments arranged by size:
2378 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 110 175 110
Size: 65 4344
BseYI C'CCAG_C
Cuts at: 1834 2592 3169 3665 1834
Size: 758 577 496 2578
Fragments arranged by size:
2578 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1951 1997 3205 104
Size: 1847 46 1208 1308
Fragments arranged by size:
1847 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1201 1423 2201 2391 3183 1201
Size: 222 778 190 792 2427
Fragments arranged by size:
2427 792 778 222 190
BsmI GAATG_Cn'
Cuts at: 176 262 1061 176
Size: 86 799 3524
Fragments arranged by size:
3524 799 86
Bsp1286I G_dGCh'C
Cuts at: 386 1201 1423 2034 2127 2201 2391 2453 3183
Size: 815 222 611 93 74 190 62 730
Cuts at: 3183 386
Size: 1612
Fragments arranged by size:
1612 815 730 611 222 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3629 3629
Size: 4409
BspHI T'CATG_A
Cuts at: 3585 3585
Size: 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1459 1673 1975 2356 1459
Size: 214 302 381 3512
Fragments arranged by size:
3512 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2701 2755 4239 4404 50
Size: 2651 54 1484 165 55
Fragments arranged by size:
2651 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2321 2321
Size: 4409
BsrFI r'CCGG_y
Cuts at: 207 2407 2588 2797 207
Size: 2200 181 209 1819
Fragments arranged by size:
2200 1819 209 181
BssSI C'ACGA_G
Cuts at: 2680 3038 2680
Size: 358 4051
BstAPI GCAn_nnn'nTGC
Cuts at: 1786 1786
Size: 4409
BstBI TT'CG_AA
Cuts at: 2770 2770
Size: 4409
BstYI r'GATC_y
Cuts at: 1 426 1434 1901 1924 2259 2505 3517 3603
Size: 425 1008 467 23 335 246 1012 86
Cuts at: 3603 3615 3961 1
Size: 12 346 449
Fragments arranged by size:
1012 1008 467 449 425 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 571 571
Size: 4409
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2359 2540 2703 2359
Size: 181 163 4065
Fragments arranged by size:
4065 181 163
BtsI GCAGTG_nn'
Cuts at: 1096 1652 1096
Size: 556 3853
DraI TTT'AAA
Cuts at: 1408 1408
Size: 4409
DrdI GACnn_nn'nnGTC
Cuts at: 479 2115 2973 479
Size: 1636 858 1915
Fragments arranged by size:
1915 1636 858
EaeI y'GGCC_r
Cuts at: 1994 2168 2559 2586 3931 1994
Size: 174 391 27 1345 2472
Fragments arranged by size:
2472 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1994 1994
Size: 4409
EarI CTCTTCn'nnn_
Cuts at: 152 463 557 2432 2642 152
Size: 311 94 1875 210 1919
Fragments arranged by size:
1919 1875 311 210 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2617 2927 3073 4219 2617
Size: 310 146 1146 2807
Fragments arranged by size:
2807 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1199 1421 1199
Size: 222 4187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 2666 3413 3681 2234
Size: 432 747 268 2962
Fragments arranged by size:
2962 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1548 1548
Size: 4409
EcoRI G'AATT_C
Cuts at: 635 887 971 1139 1307 1391 1413 635
Size: 252 84 168 168 84 22 3631
Fragments arranged by size:
3631 252 168 168 84 84 22
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4409
FspI TGC'GCA
Cuts at: 2190 2190
Size: 4409
HaeII r_GCGC'y
Cuts at: 1843 2091 3113 4092 1843
Size: 248 1022 979 2160
Fragments arranged by size:
2160 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 418 450 588 620 2251 2281 2283 2313 418
Size: 32 138 32 1631 30 2 30 2514
Fragments arranged by size:
2514 1631 138 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1448 1448
Size: 4409
KasI G'GCGC_C
Cuts at: 1839 2087 1839
Size: 248 4161
KpnI G_GTAC'C
Cuts at: 1429 1429
Size: 4409
MluI A'CGCG_T
Cuts at: 3842 3842
Size: 4409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 281 648 3080 3264 281
Size: 367 2432 184 1426
Fragments arranged by size:
2432 1426 367 184
MscI TGG'CCA
Cuts at: 2170 2170
Size: 4409
MslI CAynn'nnrTG
Cuts at: 576 644 812 896 980 1064 1148 1232 1248
Size: 68 168 84 84 84 84 84 16
Cuts at: 1248 1316 1400 2398 2525 576
Size: 68 84 998 127 2460
Fragments arranged by size:
2460 998 168 127 84 84 84 84
84 84 68 68 16
MspA1I CmG'CkG
Cuts at: 352 1520 1834 2194 3207 3452 352
Size: 1168 314 360 1013 245 1309
Fragments arranged by size:
1309 1168 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2590 2590
Size: 4409
NarI GG'CG_CC
Cuts at: 1840 2088 1840
Size: 248 4161
NcoI C'CATG_G
Cuts at: 571 571
Size: 4409
NdeI CA'TA_TG
Cuts at: 1245 1245
Size: 4409
NgoMIV G'CCGG_C
Cuts at: 2588 2588
Size: 4409
NsiI A_TGCA'T
Cuts at: 176 1061 176
Size: 885 3524
NspI r_CATG'y
Cuts at: 510 1462 1962 2869 510
Size: 952 500 907 2050
Fragments arranged by size:
2050 952 907 500
PciI A'CATG_T
Cuts at: 506 2865 506
Size: 2359 2050
PmeI GTTT'AAAC
Cuts at: 1408 1408
Size: 4409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 3548
Size: 32 4377
PstI C_TGCA'G
Cuts at: 1456 2141 1456
Size: 685 3724
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4409
PvuII CAG'CTG
Cuts at: 352 1834 2194 352
Size: 1482 360 2567
Fragments arranged by size:
2567 1482 360
RsrII CG'GwC_CG
Cuts at: 2604 2604
Size: 4409
SacI G_AGCT'C
Cuts at: 1201 1423 1201
Size: 222 4187
SalI G'TCGA_C
Cuts at: 1446 1446
Size: 4409
SapI GCTCTTCn'nnn_
Cuts at: 2432 2642 2432
Size: 210 4199
SbfI CC_TGCA'GG
Cuts at: 1456 1456
Size: 4409
ScaI AGT'ACT
Cuts at: 3926 3926
Size: 4409
SexAI A'CCwGG_T
Cuts at: 317 317
Size: 4409
SfcI C'TryA_G
Cuts at: 33 1452 2137 3130 3321 33
Size: 1419 685 993 191 1121
Fragments arranged by size:
1419 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1841 2089 1841
Size: 248 4161
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
SmaI CCC'GGG
Cuts at: 1431 1431
Size: 4409
SmlI C'TyrA_G
Cuts at: 242 1032 1564 2479 2971 3233 242
Size: 790 532 915 492 262 1418
Fragments arranged by size:
1418 915 790 532 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4409
SphI G_CATG'C
Cuts at: 1462 1962 1462
Size: 500 3909
SspI AAT'ATT
Cuts at: 455 455
Size: 4409
StuI AGG'CCT
Cuts at: 416 1102 1186 1270 416
Size: 686 84 84 3555
Fragments arranged by size:
3555 686 84 84
StyI C'CwwG_G
Cuts at: 200 571 1543 1897 200
Size: 371 972 354 2712
Fragments arranged by size:
2712 972 371 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 167 1992 2125 2584 2793 167
Size: 1825 133 459 209 1783
Fragments arranged by size:
1825 1783 459 209 133
TatI w'GTAC_w
Cuts at: 3924 3924
Size: 4409
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 424 456 605 637 3926 3958 424
Size: 32 149 32 3289 32 875
Fragments arranged by size:
3289 875 149 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2206 2206
Size: 4409
XbaI T'CTAG_A
Cuts at: 1440 1440
Size: 4409
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1904 1904
Size: 4409
XmaI C'CCGG_G
Cuts at: 1429 1429
Size: 4409
XmnI GAAnn'nnTTC
Cuts at: 1208 1208
Size: 4409
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI