Restriction map for
pF1KE0759
(Circular) (Six-base) MAPSORT of: pF1KE0759.seq Check: 8208 from: 1 to: 4106
pF1KE0759 4106 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1144 1144
Size: 4106
Acc65I G'GTAC_C
Cuts at: 1122 1122
Size: 4106
AclI AA'CG_TT
Cuts at: 3946 3946
Size: 4106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 1931 2363 3110 3378 832
Size: 1099 432 747 268 1560
Fragments arranged by size:
1560 1099 747 432 268
AflIII A'CryG_T
Cuts at: 527 1458 2562 3539 527
Size: 931 1104 977 1094
Fragments arranged by size:
1104 1094 977 931
AgeI A'CCGG_T
Cuts at: 2494 2494
Size: 4106
AlwNI CAG_nnn'CTG
Cuts at: 1411 2978 1411
Size: 1567 2539
ApaLI G'TGCA_C
Cuts at: 995 2876 995
Size: 1881 2225
ApoI r'AATT_y
Cuts at: 238 1110 3459 3715 238
Size: 872 2349 256 629
Fragments arranged by size:
2349 872 629 256
AseI AT'TA_AT
Cuts at: 20 817 20
Size: 797 3309
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
AvaI C'yCGr_G
Cuts at: 323 1126 323
Size: 803 3303
BamHI G'GATC_C
Cuts at: 1131 1131
Size: 4106
BanI G'GyrC_C
Cuts at: 1122 1536 1784 1819 1122
Size: 414 248 35 3409
Fragments arranged by size:
3409 414 248 35
BanII G_rGCy'C
Cuts at: 479 1120 2150 479
Size: 641 1030 2435
Fragments arranged by size:
2435 1030 641
BbeI G_GCGC'C
Cuts at: 1540 1788 1540
Size: 248 3858
BbsI GAAGACnn'nnnn_
Cuts at: 382 382
Size: 4106
BciVI GTATCCnnnnn_n'
Cuts at: 2004 2765 4106 2004
Size: 761 1341 2004
Fragments arranged by size:
2004 1341 761
BclI T'GATC_A
Cuts at: 1626 1626
Size: 4106
BglII A'GATC_T
Cuts at: 1621 3658 1621
Size: 2037 2069
BlpI GC'TnA_GC
Cuts at: 800 1218 800
Size: 418 3688
Bme1580I G_kGCm'C
Cuts at: 999 1731 1824 2880 999
Size: 732 93 1056 2225
Fragments arranged by size:
2225 1056 732 93
BmrI ACTGGGnnnn_n'
Cuts at: 1318 1399 1734 3878 1318
Size: 81 335 2144 1546
Fragments arranged by size:
2144 1546 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 1071 421
Size: 650 3456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1282 2161 2653 2951 1282
Size: 879 492 298 2437
Fragments arranged by size:
2437 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3810 3810
Size: 4106
BsaAI yAC'GTr
Cuts at: 147 1459 147
Size: 1312 2794
BsaBI GATnn'nnATC
Cuts at: 6 1620 3657 6
Size: 1614 2037 455
Fragments arranged by size:
2037 1614 455
BsaHI Gr'CG_yC
Cuts at: 1537 1785 2487 3983 1537
Size: 248 702 1496 1660
Fragments arranged by size:
1660 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1295 1816 2494 2768 2915 3326 1295
Size: 521 678 274 147 411 2075
Fragments arranged by size:
2075 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 654 684 654
Size: 30 4076
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 424 675 424
Size: 251 3855
BseYI C'CCAG_C
Cuts at: 1009 1040 1531 2289 2866 3362 1009
Size: 31 491 758 577 496 1753
Fragments arranged by size:
1753 758 577 496 491 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 520 277
Size: 243 3863
BsiEI CG_ry'CG
Cuts at: 104 672 1648 1694 2902 104
Size: 568 976 46 1208 1308
Fragments arranged by size:
1308 1208 976 568 46
BsiHKAI G_wGCw'C
Cuts at: 999 1120 1898 2088 2880 999
Size: 121 778 190 792 2225
Fragments arranged by size:
2225 792 778 190 121
BsmI GAATG_Cn'
Cuts at: 574 574
Size: 4106
Bsp1286I G_dGCh'C
Cuts at: 479 999 1120 1731 1824 1898 2088 2150 2880
Size: 520 121 611 93 74 190 62 730
Cuts at: 2880 479
Size: 1705
Fragments arranged by size:
1705 730 611 520 190 121 93 74
62
BspEI T'CCGG_A
Cuts at: 3326 3326
Size: 4106
BspHI T'CATG_A
Cuts at: 588 3282 588
Size: 2694 1412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1156 1370 1672 2053 1156
Size: 214 302 381 3209
Fragments arranged by size:
3209 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2398 2452 3936 4101 50
Size: 2348 54 1484 165 55
Fragments arranged by size:
2348 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2018 2018
Size: 4106
BsrFI r'CCGG_y
Cuts at: 2104 2285 2494 2104
Size: 181 209 3716
Fragments arranged by size:
3716 209 181
BssSI C'ACGA_G
Cuts at: 992 2377 2735 992
Size: 1385 358 2363
Fragments arranged by size:
2363 1385 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1483 1483
Size: 4106
BstBI TT'CG_AA
Cuts at: 2467 2467
Size: 4106
BstYI r'GATC_y
Cuts at: 1 1131 1598 1621 1956 2202 3214 3300 3312
Size: 1130 467 23 335 246 1012 86 12
Cuts at: 3312 3658 1
Size: 346 449
Fragments arranged by size:
1130 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2056 2237 2400 2056
Size: 181 163 3762
Fragments arranged by size:
3762 181 163
BtsI GCAGTG_nn'
Cuts at: 1349 1349
Size: 4106
DraI TTT'AAA
Cuts at: 1105 1105
Size: 4106
DrdI GACnn_nn'nnGTC
Cuts at: 1812 2670 1812
Size: 858 3248
EaeI y'GGCC_r
Cuts at: 488 791 1691 1865 2256 2283 3628 488
Size: 303 900 174 391 27 1345 966
Fragments arranged by size:
1345 966 900 391 303 174 27
EagI C'GGCC_G
Cuts at: 1691 1691
Size: 4106
EarI CTCTTCn'nnn_
Cuts at: 899 1028 2129 2339 899
Size: 129 1101 210 2666
Fragments arranged by size:
2666 1101 210 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2314 2624 2770 3916 2314
Size: 310 146 1146 2504
Fragments arranged by size:
2504 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1118 1118
Size: 4106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 832 1071 1931 2363 3110 3378 421
Size: 411 239 860 432 747 268 1149
Fragments arranged by size:
1149 860 747 432 411 268 239
EcoO109I rG'GnC_Cy
Cuts at: 220 1245 220
Size: 1025 3081
EcoRI G'AATT_C
Cuts at: 1110 1110
Size: 4106
EcoRV GAT'ATC
Cuts at: 612 612
Size: 4106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 4106
FspI TGC'GCA
Cuts at: 1887 1887
Size: 4106
HaeII r_GCGC'y
Cuts at: 1540 1788 2810 3789 1540
Size: 248 1022 979 1857
Fragments arranged by size:
1857 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 355 387 630 654 662 686 746 778 1948
Size: 32 243 24 8 24 60 32 1170
Cuts at: 1948 1978 1980 2010 355
Size: 30 2 30 2451
Fragments arranged by size:
2451 1170 243 60 32 32 30 30
24 24 8 2
HincII GTy'rAC
Cuts at: 1145 1145
Size: 4106
HindIII A'AGCT_T
Cuts at: 166 855 166
Size: 689 3417
KasI G'GCGC_C
Cuts at: 1536 1784 1536
Size: 248 3858
KpnI G_GTAC'C
Cuts at: 1126 1126
Size: 4106
MluI A'CGCG_T
Cuts at: 3539 3539
Size: 4106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 140 2777 2961 140
Size: 2637 184 1285
Fragments arranged by size:
2637 1285 184
MscI TGG'CCA
Cuts at: 793 1867 793
Size: 1074 3032
MslI CAynn'nnrTG
Cuts at: 2095 2222 2095
Size: 127 3979
MspA1I CmG'CkG
Cuts at: 504 1217 1531 1891 2904 3149 504
Size: 713 314 360 1013 245 1461
Fragments arranged by size:
1461 1013 713 360 314 245
NaeI GCC'GGC
Cuts at: 2287 2287
Size: 4106
NarI GG'CG_CC
Cuts at: 1537 1785 1537
Size: 248 3858
NcoI C'CATG_G
Cuts at: 107 107
Size: 4106
NgoMIV G'CCGG_C
Cuts at: 2285 2285
Size: 4106
NspI r_CATG'y
Cuts at: 343 1159 1659 2566 343
Size: 816 500 907 1883
Fragments arranged by size:
1883 907 816 500
PciI A'CATG_T
Cuts at: 2562 2562
Size: 4106
PmeI GTTT'AAAC
Cuts at: 1105 1105
Size: 4106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 4074
PpuMI rG'GwC_Cy
Cuts at: 220 220
Size: 4106
PstI C_TGCA'G
Cuts at: 1153 1838 1153
Size: 685 3421
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4106
PvuII CAG'CTG
Cuts at: 1531 1891 1531
Size: 360 3746
RsrII CG'GwC_CG
Cuts at: 2301 2301
Size: 4106
SacI G_AGCT'C
Cuts at: 1120 1120
Size: 4106
SalI G'TCGA_C
Cuts at: 1143 1143
Size: 4106
SapI GCTCTTCn'nnn_
Cuts at: 2129 2339 2129
Size: 210 3896
SbfI CC_TGCA'GG
Cuts at: 1153 1153
Size: 4106
ScaI AGT'ACT
Cuts at: 3623 3623
Size: 4106
SfcI C'TryA_G
Cuts at: 33 951 1080 1149 1834 2827 3018 33
Size: 918 129 69 685 993 191 1121
Fragments arranged by size:
1121 993 918 685 191 129 69
SfoI GGC'GCC
Cuts at: 1538 1786 1538
Size: 248 3858
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
SmaI CCC'GGG
Cuts at: 1128 1128
Size: 4106
SmlI C'TyrA_G
Cuts at: 1261 2176 2668 2930 1261
Size: 915 492 262 2437
Fragments arranged by size:
2437 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4106
SphI G_CATG'C
Cuts at: 343 1159 1659 343
Size: 816 500 2790
Fragments arranged by size:
2790 816 500
StyI C'CwwG_G
Cuts at: 107 468 623 1240 1594 107
Size: 361 155 617 354 2619
Fragments arranged by size:
2619 617 361 354 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1689 1822 2281 2490 1689
Size: 133 459 209 3305
Fragments arranged by size:
3305 459 209 133
TatI w'GTAC_w
Cuts at: 3621 3621
Size: 4106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 667 699 3623 3655 667
Size: 32 2924 32 1118
Fragments arranged by size:
2924 1118 32 32
Tth111I GACn'n_nGTC
Cuts at: 130 1903 130
Size: 1773 2333
XbaI T'CTAG_A
Cuts at: 1137 1137
Size: 4106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 630 1601 630
Size: 971 3135
XmaI C'CCGG_G
Cuts at: 1126 1126
Size: 4106
XmnI GAAnn'nnTTC
Cuts at: 239 239
Size: 4106
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI