Restriction map for
pF1KE0758
(Circular) (Six-base) MAPSORT of: pF1KE0758.seq Check: 8959 from: 1 to: 4409
pF1KE0758 4409 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AccI GT'mk_AC
Cuts at: 1447 1447
Size: 4409
Acc65I G'GTAC_C
Cuts at: 1425 1425
Size: 4409
AclI AA'CG_TT
Cuts at: 4249 4249
Size: 4409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 816 900 1068 1320 1374 2234 2666 3413 3681
Size: 84 168 252 54 860 432 747 268
Cuts at: 3681 816
Size: 1544
Fragments arranged by size:
1544 860 747 432 268 252 168 84
54
AflIII A'CryG_T
Cuts at: 858 1761 2865 3842 858
Size: 903 1104 977 1425
Fragments arranged by size:
1425 1104 977 903
AgeI A'CCGG_T
Cuts at: 2797 2797
Size: 4409
AlwNI CAG_nnn'CTG
Cuts at: 1714 3281 1714
Size: 1567 2842
ApaLI G'TGCA_C
Cuts at: 3179 3179
Size: 4409
ApoI r'AATT_y
Cuts at: 698 782 866 1286 1413 3762 4018 698
Size: 84 84 420 127 2349 256 1089
Fragments arranged by size:
2349 1089 420 256 127 84 84
AseI AT'TA_AT
Cuts at: 20 497 20
Size: 477 3932
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
AvaI C'yCGr_G
Cuts at: 1429 1429
Size: 4409
BamHI G'GATC_C
Cuts at: 1434 1434
Size: 4409
BanI G'GyrC_C
Cuts at: 1425 1839 2087 2122 1425
Size: 414 248 35 3712
Fragments arranged by size:
3712 414 248 35
BanII G_rGCy'C
Cuts at: 1423 2453 1423
Size: 1030 3379
BbeI G_GCGC'C
Cuts at: 1843 2091 1843
Size: 248 4161
BbsI GAAGACnn'nnnn_
Cuts at: 526 526
Size: 4409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4375
BciVI GTATCCnnnnn_n'
Cuts at: 2307 3068 4409 2307
Size: 761 1341 2307
Fragments arranged by size:
2307 1341 761
BclI T'GATC_A
Cuts at: 1929 1929
Size: 4409
BglII A'GATC_T
Cuts at: 1924 3961 1924
Size: 2037 2372
BlpI GC'TnA_GC
Cuts at: 1521 1521
Size: 4409
Bme1580I G_kGCm'C
Cuts at: 2034 2127 3183 2034
Size: 93 1056 3260
Fragments arranged by size:
3260 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 168 1621 1702 2037 4181 168
Size: 1453 81 335 2144 396
Fragments arranged by size:
2144 1453 396 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2776 2776
Size: 4409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 1148 1232 1316 133
Size: 1015 84 84 3226
Fragments arranged by size:
3226 1015 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1585 2464 2956 3254 1585
Size: 879 492 298 2740
Fragments arranged by size:
2740 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 138 4113 138
Size: 3975 434
BsaAI yAC'GTr
Cuts at: 1762 1762
Size: 4409
BsaBI GATnn'nnATC
Cuts at: 6 1923 3960 6
Size: 1917 2037 455
Fragments arranged by size:
2037 1917 455
BsaHI Gr'CG_yC
Cuts at: 1840 2088 2790 4286 1840
Size: 248 702 1496 1963
Fragments arranged by size:
1963 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1598 2119 2797 3071 3218 3629 1598
Size: 521 678 274 147 411 2378
Fragments arranged by size:
2378 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1834 2592 3169 3665 1834
Size: 758 577 496 2578
Fragments arranged by size:
2578 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1951 1997 3205 104
Size: 1847 46 1208 1308
Fragments arranged by size:
1847 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1423 2201 2391 3183 1423
Size: 778 190 792 2649
Fragments arranged by size:
2649 792 778 190
BsmI GAATG_Cn'
Cuts at: 1234 1234
Size: 4409
Bsp1286I G_dGCh'C
Cuts at: 1423 2034 2127 2201 2391 2453 3183 1423
Size: 611 93 74 190 62 730 2649
Fragments arranged by size:
2649 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3629 3629
Size: 4409
BspHI T'CATG_A
Cuts at: 3585 3585
Size: 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1459 1673 1975 2356 1459
Size: 214 302 381 3512
Fragments arranged by size:
3512 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2701 2755 4239 4404 50
Size: 2651 54 1484 165 55
Fragments arranged by size:
2651 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1401 2321 1401
Size: 920 3489
BsrFI r'CCGG_y
Cuts at: 2407 2588 2797 2407
Size: 181 209 4019
Fragments arranged by size:
4019 209 181
BssSI C'ACGA_G
Cuts at: 2680 3038 2680
Size: 358 4051
BstAPI GCAn_nnn'nTGC
Cuts at: 1786 1786
Size: 4409
BstBI TT'CG_AA
Cuts at: 2770 2770
Size: 4409
BstXI CCAn_nnnn'nTGG
Cuts at: 928 928
Size: 4409
BstYI r'GATC_y
Cuts at: 1 1434 1901 1924 2259 2505 3517 3603 3615
Size: 1433 467 23 335 246 1012 86 12
Cuts at: 3615 3961 1
Size: 346 449
Fragments arranged by size:
1433 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1018 1018
Size: 4409
BtgI C'CryG_G
Cuts at: 196 196
Size: 4409
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 830 2359 2540 2703 830
Size: 1529 181 163 2536
Fragments arranged by size:
2536 1529 181 163
BtsI GCAGTG_nn'
Cuts at: 1362 1652 1362
Size: 290 4119
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 969 1004 969
Size: 35 4374
DraI TTT'AAA
Cuts at: 1408 1408
Size: 4409
DrdI GACnn_nn'nnGTC
Cuts at: 530 2115 2973 530
Size: 1585 858 1966
Fragments arranged by size:
1966 1585 858
EaeI y'GGCC_r
Cuts at: 1994 2168 2559 2586 3931 1994
Size: 174 391 27 1345 2472
Fragments arranged by size:
2472 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1994 1994
Size: 4409
EarI CTCTTCn'nnn_
Cuts at: 238 2432 2642 238
Size: 2194 210 2005
Fragments arranged by size:
2194 2005 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2617 2927 3073 4219 2617
Size: 310 146 1146 2807
Fragments arranged by size:
2807 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1421 1421
Size: 4409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 816 900 1068 1148 1232 1316 1320 1374
Size: 683 84 168 80 84 84 4 54
Cuts at: 1374 2234 2666 3413 3681 133
Size: 860 432 747 268 861
Fragments arranged by size:
861 860 747 683 432 268 168 84
84 84 80 54 4
EcoO109I rG'GnC_Cy
Cuts at: 1548 1548
Size: 4409
EcoRI G'AATT_C
Cuts at: 698 782 866 1286 1413 698
Size: 84 84 420 127 3694
Fragments arranged by size:
3694 420 127 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4409
FspI TGC'GCA
Cuts at: 2190 2190
Size: 4409
HaeII r_GCGC'y
Cuts at: 1843 2091 3113 4092 1843
Size: 248 1022 979 2160
Fragments arranged by size:
2160 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1167 1199 2251 2281 2283 2313 1167
Size: 32 1052 30 2 30 3263
Fragments arranged by size:
3263 1052 32 30 30 2
HincII GTy'rAC
Cuts at: 1448 1448
Size: 4409
KasI G'GCGC_C
Cuts at: 1839 2087 1839
Size: 248 4161
KpnI G_GTAC'C
Cuts at: 1429 1429
Size: 4409
MluI A'CGCG_T
Cuts at: 3842 3842
Size: 4409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 158 3080 3264 158
Size: 2922 184 1303
Fragments arranged by size:
2922 1303 184
MscI TGG'CCA
Cuts at: 2170 2170
Size: 4409
MslI CAynn'nnrTG
Cuts at: 791 1043 1143 2398 2525 791
Size: 252 100 1255 127 2675
Fragments arranged by size:
2675 1255 252 127 100
MspA1I CmG'CkG
Cuts at: 1340 1520 1834 2194 3207 3452 1340
Size: 180 314 360 1013 245 2297
Fragments arranged by size:
2297 1013 360 314 245 180
NaeI GCC'GGC
Cuts at: 2590 2590
Size: 4409
NarI GG'CG_CC
Cuts at: 1840 2088 1840
Size: 248 4161
NcoI C'CATG_G
Cuts at: 196 196
Size: 4409
NgoMIV G'CCGG_C
Cuts at: 2588 2588
Size: 4409
NspI r_CATG'y
Cuts at: 348 862 980 1462 1962 2869 348
Size: 514 118 482 500 907 1888
Fragments arranged by size:
1888 907 514 500 482 118
PciI A'CATG_T
Cuts at: 858 2865 858
Size: 2007 2402
PflMI CCAn_nnn'nTGG
Cuts at: 411 411
Size: 4409
PmeI GTTT'AAAC
Cuts at: 1408 1408
Size: 4409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 118 150 3548 3580 118
Size: 32 3398 32 947
Fragments arranged by size:
3398 947 32 32
PstI C_TGCA'G
Cuts at: 1456 2141 1456
Size: 685 3724
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4409
PvuII CAG'CTG
Cuts at: 1340 1834 2194 1340
Size: 494 360 3555
Fragments arranged by size:
3555 494 360
RsrII CG'GwC_CG
Cuts at: 2604 2604
Size: 4409
SacI G_AGCT'C
Cuts at: 1423 1423
Size: 4409
SalI G'TCGA_C
Cuts at: 1446 1446
Size: 4409
SapI GCTCTTCn'nnn_
Cuts at: 238 2432 2642 238
Size: 2194 210 2005
Fragments arranged by size:
2194 2005 210
SbfI CC_TGCA'GG
Cuts at: 1456 1456
Size: 4409
ScaI AGT'ACT
Cuts at: 3926 3926
Size: 4409
SexAI A'CCwGG_T
Cuts at: 128 128
Size: 4409
SfcI C'TryA_G
Cuts at: 33 1341 1452 2137 3130 3321 33
Size: 1308 111 685 993 191 1121
Fragments arranged by size:
1308 1121 993 685 191 111
SfoI GGC'GCC
Cuts at: 1841 2089 1841
Size: 248 4161
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
SmaI CCC'GGG
Cuts at: 1431 1431
Size: 4409
SmlI C'TyrA_G
Cuts at: 1564 2479 2971 3233 1564
Size: 915 492 262 2740
Fragments arranged by size:
2740 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4409
SphI G_CATG'C
Cuts at: 1462 1962 1462
Size: 500 3909
SspI AAT'ATT
Cuts at: 648 648
Size: 4409
StyI C'CwwG_G
Cuts at: 196 356 1543 1897 196
Size: 160 1187 354 2708
Fragments arranged by size:
2708 1187 354 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1992 2125 2584 2793 1992
Size: 133 459 209 3608
Fragments arranged by size:
3608 459 209 133
TatI w'GTAC_w
Cuts at: 1093 3924 1093
Size: 2831 1578
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3926 3958 3926
Size: 32 4377
Tth111I GACn'n_nGTC
Cuts at: 2206 2206
Size: 4409
XbaI T'CTAG_A
Cuts at: 1440 1440
Size: 4409
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1904 1904
Size: 4409
XmaI C'CCGG_G
Cuts at: 1429 1429
Size: 4409
XmnI GAAnn'nnTTC
Cuts at: 448 718 829 913 448
Size: 270 111 84 3944
Fragments arranged by size:
3944 270 111 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI