Restriction map for
pF1KE0757
(Circular) (Six-base) MAPSORT of: pF1KE0757.seq Check: 2654 from: 1 to: 4103
pF1KE0757 4103 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 659 1141 659
Size: 482 3621
Acc65I G'GTAC_C
Cuts at: 1119 1119
Size: 4103
AclI AA'CG_TT
Cuts at: 3943 3943
Size: 4103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 1928 2360 3107 3375 159
Size: 1769 432 747 268 887
Fragments arranged by size:
1769 887 747 432 268
AfeI AGC'GCT
Cuts at: 615 615
Size: 4103
AflIII A'CryG_T
Cuts at: 1455 2559 3536 1455
Size: 1104 977 2022
Fragments arranged by size:
2022 1104 977
AgeI A'CCGG_T
Cuts at: 2491 2491
Size: 4103
AlwNI CAG_nnn'CTG
Cuts at: 368 861 956 1408 2975 368
Size: 493 95 452 1567 1496
Fragments arranged by size:
1567 1496 493 452 95
ApaLI G'TGCA_C
Cuts at: 870 2873 870
Size: 2003 2100
ApoI r'AATT_y
Cuts at: 1107 3456 3712 1107
Size: 2349 256 1498
Fragments arranged by size:
2349 1498 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4103
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
AvaI C'yCGr_G
Cuts at: 787 865 934 1045 1123 787
Size: 78 69 111 78 3767
Fragments arranged by size:
3767 111 78 78 69
BamHI G'GATC_C
Cuts at: 410 1128 410
Size: 718 3385
BanI G'GyrC_C
Cuts at: 469 898 1119 1533 1781 1816 469
Size: 429 221 414 248 35 2756
Fragments arranged by size:
2756 429 414 248 221 35
BanII G_rGCy'C
Cuts at: 284 670 707 722 1117 2147 284
Size: 386 37 15 395 1030 2240
Fragments arranged by size:
2240 1030 395 386 37 15
BbeI G_GCGC'C
Cuts at: 1537 1785 1537
Size: 248 3855
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4069
BciVI GTATCCnnnnn_n'
Cuts at: 2001 2762 4103 2001
Size: 761 1341 2001
Fragments arranged by size:
2001 1341 761
BclI T'GATC_A
Cuts at: 1623 1623
Size: 4103
BglI GCCn_nnn'nGGC
Cuts at: 161 161
Size: 4103
BglII A'GATC_T
Cuts at: 1618 3655 1618
Size: 2037 2066
BlpI GC'TnA_GC
Cuts at: 844 1215 844
Size: 371 3732
Bme1580I G_kGCm'C
Cuts at: 241 381 874 901 1728 1821 2877 241
Size: 140 493 27 827 93 1056 1467
Fragments arranged by size:
1467 1056 827 493 140 93 27
BmgBI CAC'GTC
Cuts at: 1059 1059
Size: 4103
BmrI ACTGGGnnnn_n'
Cuts at: 368 488 1315 1396 1731 3875 368
Size: 120 827 81 335 2144 596
Fragments arranged by size:
2144 827 596 335 120 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 316
Size: 4103
Bpu10I CC'TnA_GC
Cuts at: 398 398
Size: 4103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 955 1279 2158 2650 2948 796
Size: 159 324 879 492 298 1951
Fragments arranged by size:
1951 879 492 324 298 159
BsaI GGTCTCn'nnnn_
Cuts at: 239 3807 239
Size: 3568 535
BsaAI yAC'GTr
Cuts at: 1456 1456
Size: 4103
BsaBI GATnn'nnATC
Cuts at: 6 415 1617 3654 6
Size: 409 1202 2037 455
Fragments arranged by size:
2037 1202 455 409
BsaHI Gr'CG_yC
Cuts at: 1534 1782 2484 3980 1534
Size: 248 702 1496 1657
Fragments arranged by size:
1657 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1292 1813 2491 2765 2912 3323 1292
Size: 521 678 274 147 411 2072
Fragments arranged by size:
2072 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 652 682 652
Size: 30 4073
BseYI C'CCAG_C
Cuts at: 610 621 831 1528 2286 2863 3359 610
Size: 11 210 697 758 577 496 1354
Fragments arranged by size:
1354 758 697 577 496 210 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 969 969
Size: 4103
BsiEI CG_ry'CG
Cuts at: 104 168 1645 1691 2899 104
Size: 64 1477 46 1208 1308
Fragments arranged by size:
1477 1308 1208 64 46
BsiHKAI G_wGCw'C
Cuts at: 277 284 589 874 1117 1895 2085 2877 277
Size: 7 305 285 243 778 190 792 1503
Fragments arranged by size:
1503 792 778 305 285 243 190 7
Bsp1286I G_dGCh'C
Cuts at: 241 277 284 381 589 670 707 722 874
Size: 36 7 97 208 81 37 15 152
Cuts at: 874 901 1117 1728 1821 1895 2085 2147 2877
Size: 27 216 611 93 74 190 62 730
Cuts at: 2877 241
Size: 1467
Fragments arranged by size:
1467 730 611 216 208 190 152 97
93 81 74 62 37 36 27 15
7
BspEI T'CCGG_A
Cuts at: 3323 3323
Size: 4103
BspHI T'CATG_A
Cuts at: 284 3279 284
Size: 2995 1108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1153 1367 1669 2050 1153
Size: 214 302 381 3206
Fragments arranged by size:
3206 381 302 214
BsrBI CCG'CTC
Cuts at: 50 848 1002 2395 2449 3933 4098 50
Size: 798 154 1393 54 1484 165 55
Fragments arranged by size:
1484 1393 798 165 154 55 54
BsrDI GCAATG_nn'
Cuts at: 2015 2015
Size: 4103
BsrFI r'CCGG_y
Cuts at: 766 2101 2282 2491 766
Size: 1335 181 209 2378
Fragments arranged by size:
2378 1335 209 181
BsrGI T'GTAC_A
Cuts at: 677 677
Size: 4103
BssHII G'CGCG_C
Cuts at: 179 1030 179
Size: 851 3252
BssSI C'ACGA_G
Cuts at: 2374 2732 2374
Size: 358 3745
BstAPI GCAn_nnn'nTGC
Cuts at: 229 756 861 878 956 1480 229
Size: 527 105 17 78 524 2852
Fragments arranged by size:
2852 527 524 105 78 17
BstBI TT'CG_AA
Cuts at: 2464 2464
Size: 4103
BstXI CCAn_nnnn'nTGG
Cuts at: 831 831
Size: 4103
BstYI r'GATC_y
Cuts at: 1 410 428 508 1128 1595 1618 1953 2199
Size: 409 18 80 620 467 23 335 246
Cuts at: 2199 3211 3297 3309 3655 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 620 467 449 409 346 335 246
86 80 23 18 12
Bsu36I CC'TnA_GG
Cuts at: 356 465 356
Size: 109 3994
BtgI C'CryG_G
Cuts at: 637 707 713 818 885 637
Size: 70 6 105 67 3855
Fragments arranged by size:
3855 105 70 67 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2053 2234 2397 2053
Size: 181 163 3759
Fragments arranged by size:
3759 181 163
BtsI GCAGTG_nn'
Cuts at: 957 1346 957
Size: 389 3714
ClaI AT'CG_AT
Cuts at: 419 419
Size: 4103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 960 995 960
Size: 35 4068
DraI TTT'AAA
Cuts at: 1102 1102
Size: 4103
DrdI GACnn_nn'nnGTC
Cuts at: 655 1809 2667 655
Size: 1154 858 2091
Fragments arranged by size:
2091 1154 858
EaeI y'GGCC_r
Cuts at: 165 710 821 1688 1862 2253 2280 3625 165
Size: 545 111 867 174 391 27 1345 643
Fragments arranged by size:
1345 867 643 545 391 174 111 27
EagI C'GGCC_G
Cuts at: 165 1688 165
Size: 1523 2580
EarI CTCTTCn'nnn_
Cuts at: 2126 2336 2126
Size: 210 3893
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 142 2311 2621 2767 3913 142
Size: 2169 310 146 1146 332
Fragments arranged by size:
2169 1146 332 310 146
EcoICRI GAG'CTC
Cuts at: 282 1115 282
Size: 833 3270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 316 1928 2360 3107 3375 159
Size: 157 1612 432 747 268 887
Fragments arranged by size:
1612 887 747 432 268 157
EcoNI CCTnn'n_nnAGG
Cuts at: 253 253
Size: 4103
EcoO109I rG'GnC_Cy
Cuts at: 1242 1242
Size: 4103
EcoRI G'AATT_C
Cuts at: 1107 1107
Size: 4103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 4103
FspI TGC'GCA
Cuts at: 1884 1884
Size: 4103
HaeII r_GCGC'y
Cuts at: 617 839 1537 1785 2807 3786 617
Size: 222 698 248 1022 979 934
Fragments arranged by size:
1022 979 934 698 248 222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 236 268 652 684 1945 1975 1977 2007 236
Size: 32 384 32 1261 30 2 30 2332
Fragments arranged by size:
2332 1261 384 32 32 30 30 2
HincII GTy'rAC
Cuts at: 660 1142 660
Size: 482 3621
KasI G'GCGC_C
Cuts at: 1533 1781 1533
Size: 248 3855
KpnI G_GTAC'C
Cuts at: 1123 1123
Size: 4103
MluI A'CGCG_T
Cuts at: 3536 3536
Size: 4103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2774 2958 2774
Size: 184 3919
MscI TGG'CCA
Cuts at: 1864 1864
Size: 4103
MslI CAynn'nnrTG
Cuts at: 2092 2219 2092
Size: 127 3976
MspA1I CmG'CkG
Cuts at: 639 715 843 1214 1528 1888 2901 3146 639
Size: 76 128 371 314 360 1013 245 1596
Fragments arranged by size:
1596 1013 371 360 314 245 128 76
NaeI GCC'GGC
Cuts at: 768 2284 768
Size: 1516 2587
NarI GG'CG_CC
Cuts at: 1534 1782 1534
Size: 248 3855
NdeI CA'TA_TG
Cuts at: 516 516
Size: 4103
NgoMIV G'CCGG_C
Cuts at: 766 2282 766
Size: 1516 2587
NspI r_CATG'y
Cuts at: 598 759 1156 1656 2563 598
Size: 161 397 500 907 2138
Fragments arranged by size:
2138 907 500 397 161
PasI CC'CwG_GG
Cuts at: 143 143
Size: 4103
PciI A'CATG_T
Cuts at: 2559 2559
Size: 4103
PflMI CCAn_nnn'nTGG
Cuts at: 490 490
Size: 4103
PfoI T'CCnGG_A
Cuts at: 431 431
Size: 4103
PmeI GTTT'AAAC
Cuts at: 1102 1102
Size: 4103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 4071
PstI C_TGCA'G
Cuts at: 655 857 1150 1835 655
Size: 202 293 685 2923
Fragments arranged by size:
2923 685 293 202
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4103
PvuII CAG'CTG
Cuts at: 1528 1888 1528
Size: 360 3743
RsrII CG'GwC_CG
Cuts at: 2298 2298
Size: 4103
SacI G_AGCT'C
Cuts at: 284 1117 284
Size: 833 3270
SacII CC_GC'GG
Cuts at: 640 716 640
Size: 76 4027
SalI G'TCGA_C
Cuts at: 658 1140 658
Size: 482 3621
SapI GCTCTTCn'nnn_
Cuts at: 2126 2336 2126
Size: 210 3893
SbfI CC_TGCA'GG
Cuts at: 857 1150 857
Size: 293 3810
ScaI AGT'ACT
Cuts at: 3620 3620
Size: 4103
SfcI C'TryA_G
Cuts at: 33 651 853 1146 1831 2824 3015 33
Size: 618 202 293 685 993 191 1121
Fragments arranged by size:
1121 993 685 618 293 202 191
SfoI GGC'GCC
Cuts at: 1535 1783 1535
Size: 248 3855
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
SmaI CCC'GGG
Cuts at: 789 1125 789
Size: 336 3767
SmlI C'TyrA_G
Cuts at: 811 970 1258 2173 2665 2927 811
Size: 159 288 915 492 262 1987
Fragments arranged by size:
1987 915 492 288 262 159
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4103
SphI G_CATG'C
Cuts at: 759 1156 1656 759
Size: 397 500 3206
Fragments arranged by size:
3206 500 397
SrfI GCCC'GGGC
Cuts at: 789 789
Size: 4103
StuI AGG'CCT
Cuts at: 354 632 354
Size: 278 3825
StyI C'CwwG_G
Cuts at: 1025 1082 1237 1591 1025
Size: 57 155 354 3537
Fragments arranged by size:
3537 354 155 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1686 1819 2278 2487 1686
Size: 133 459 209 3302
Fragments arranged by size:
3302 459 209 133
TatI w'GTAC_w
Cuts at: 677 3618 677
Size: 2941 1162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 395 427 3620 3652 395
Size: 32 3193 32 846
Fragments arranged by size:
3193 846 32 32
Tth111I GACn'n_nGTC
Cuts at: 1900 1900
Size: 4103
XbaI T'CTAG_A
Cuts at: 1134 1134
Size: 4103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 262 1083 1598 262
Size: 821 515 2767
Fragments arranged by size:
2767 821 515
XmaI C'CCGG_G
Cuts at: 787 1123 787
Size: 336 3767
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmtI BseRI
BsiWI BsmI BsmBI BstEII BstZ17I DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NheI NotI NruI
NsiI PacI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SspI SwaI
XhoI XmnI ZraI