Restriction map for
pF1KE0754
(Circular) (Six-base) MAPSORT of: pF1KE0754.seq Check: 6277 from: 1 to: 4403
pF1KE0754 4403 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AccI GT'mk_AC
Cuts at: 1441 1441
Size: 4403
Acc65I G'GTAC_C
Cuts at: 1419 1419
Size: 4403
AclI AA'CG_TT
Cuts at: 4243 4243
Size: 4403
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 730 2228 2660 3407 3675 270
Size: 460 1498 432 747 268 998
Fragments arranged by size:
1498 998 747 460 432 268
AfeI AGC'GCT
Cuts at: 480 480
Size: 4403
AflIII A'CryG_T
Cuts at: 691 1755 2859 3836 691
Size: 1064 1104 977 1258
Fragments arranged by size:
1258 1104 1064 977
AgeI A'CCGG_T
Cuts at: 265 2791 265
Size: 2526 1877
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 428 428
Size: 4403
AlwNI CAG_nnn'CTG
Cuts at: 1126 1708 3275 1126
Size: 582 1567 2254
Fragments arranged by size:
2254 1567 582
ApaI G_GGCC'C
Cuts at: 903 903
Size: 4403
ApaLI G'TGCA_C
Cuts at: 3173 3173
Size: 4403
ApoI r'AATT_y
Cuts at: 509 540 620 678 815 1207 1221 1407 3756
Size: 31 80 58 137 392 14 186 2349
Cuts at: 3756 4012 509
Size: 256 900
Fragments arranged by size:
2349 900 392 256 186 137 80 58
31 14
AseI AT'TA_AT
Cuts at: 20 839 20
Size: 819 3584
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4403
AvaI C'yCGr_G
Cuts at: 1423 1423
Size: 4403
BamHI G'GATC_C
Cuts at: 1428 1428
Size: 4403
BanI G'GyrC_C
Cuts at: 262 1419 1833 2081 2116 262
Size: 1157 414 248 35 2549
Fragments arranged by size:
2549 1157 414 248 35
BanII G_rGCy'C
Cuts at: 302 903 1417 2447 302
Size: 601 514 1030 2258
Fragments arranged by size:
2258 1030 601 514
BbeI G_GCGC'C
Cuts at: 1837 2085 1837
Size: 248 4155
BbsI GAAGACnn'nnnn_
Cuts at: 206 599 206
Size: 393 4010
BciVI GTATCCnnnnn_n'
Cuts at: 2301 3062 4403 2301
Size: 761 1341 2301
Fragments arranged by size:
2301 1341 761
BclI T'GATC_A
Cuts at: 825 1923 825
Size: 1098 3305
BglII A'GATC_T
Cuts at: 1918 3955 1918
Size: 2037 2366
BlpI GC'TnA_GC
Cuts at: 1515 1515
Size: 4403
Bme1580I G_kGCm'C
Cuts at: 903 2028 2121 3177 903
Size: 1125 93 1056 2129
Fragments arranged by size:
2129 1125 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1615 1696 2031 4175 1615
Size: 81 335 2144 1843
Fragments arranged by size:
2144 1843 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 952 2770 952
Size: 1818 2585
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 316
Size: 4403
Bpu10I CC'TnA_GC
Cuts at: 1256 1256
Size: 4403
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1579 2458 2950 3248 1579
Size: 879 492 298 2734
Fragments arranged by size:
2734 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 940 4107 940
Size: 3167 1236
BsaAI yAC'GTr
Cuts at: 321 1756 321
Size: 1435 2968
BsaBI GATnn'nnATC
Cuts at: 6 1917 3954 6
Size: 1911 2037 455
Fragments arranged by size:
2037 1911 455
BsaHI Gr'CG_yC
Cuts at: 277 1371 1834 2082 2784 4280 277
Size: 1094 463 248 702 1496 400
Fragments arranged by size:
1496 1094 702 463 400 248
BsaWI w'CCGG_w
Cuts at: 265 1592 2113 2791 3065 3212 3623 265
Size: 1327 521 678 274 147 411 1045
Fragments arranged by size:
1327 1045 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 379 877 379
Size: 498 3905
BseYI C'CCAG_C
Cuts at: 1828 2586 3163 3659 1828
Size: 758 577 496 2572
Fragments arranged by size:
2572 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 270 1945 1991 3199 104
Size: 166 1675 46 1208 1308
Fragments arranged by size:
1675 1308 1208 166 46
BsiHKAI G_wGCw'C
Cuts at: 1109 1417 2195 2385 3177 1109
Size: 308 778 190 792 2335
Fragments arranged by size:
2335 792 778 308 190
Bsp1286I G_dGCh'C
Cuts at: 302 903 1109 1417 2028 2121 2195 2385 2447
Size: 601 206 308 611 93 74 190 62
Cuts at: 2447 3177 302
Size: 730 1528
Fragments arranged by size:
1528 730 611 601 308 206 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3623 3623
Size: 4403
BspHI T'CATG_A
Cuts at: 3579 3579
Size: 4403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 420 1453 1667 1969 2350 420
Size: 1033 214 302 381 2473
Fragments arranged by size:
2473 1033 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2695 2749 4233 4398 50
Size: 2645 54 1484 165 55
Fragments arranged by size:
2645 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2315 2315
Size: 4403
BsrFI r'CCGG_y
Cuts at: 265 2401 2582 2791 265
Size: 2136 181 209 1877
Fragments arranged by size:
2136 1877 209 181
BssSI C'ACGA_G
Cuts at: 2674 3032 2674
Size: 358 4045
BstAPI GCAn_nnn'nTGC
Cuts at: 1780 1780
Size: 4403
BstBI TT'CG_AA
Cuts at: 2764 2764
Size: 4403
BstXI CCAn_nnnn'nTGG
Cuts at: 114 795 114
Size: 681 3722
BstYI r'GATC_y
Cuts at: 1 1428 1895 1918 2253 2499 3511 3597 3609
Size: 1427 467 23 335 246 1012 86 12
Cuts at: 3609 3955 1
Size: 346 449
Fragments arranged by size:
1427 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4403
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2353 2534 2697 2353
Size: 181 163 4059
Fragments arranged by size:
4059 181 163
BtsI GCAGTG_nn'
Cuts at: 1646 1646
Size: 4403
DraI TTT'AAA
Cuts at: 723 1212 1402 723
Size: 489 190 3724
Fragments arranged by size:
3724 489 190
DrdI GACnn_nn'nnGTC
Cuts at: 601 2109 2967 601
Size: 1508 858 2037
Fragments arranged by size:
2037 1508 858
EaeI y'GGCC_r
Cuts at: 1049 1988 2162 2553 2580 3925 1049
Size: 939 174 391 27 1345 1527
Fragments arranged by size:
1527 1345 939 391 174 27
EagI C'GGCC_G
Cuts at: 1988 1988
Size: 4403
EarI CTCTTCn'nnn_
Cuts at: 130 289 635 2426 2636 130
Size: 159 346 1791 210 1897
Fragments arranged by size:
1897 1791 346 210 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 182 2611 2921 3067 4213 182
Size: 2429 310 146 1146 372
Fragments arranged by size:
2429 1146 372 310 146
EcoICRI GAG'CTC
Cuts at: 1415 1415
Size: 4403
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 316 730 2228 2660 3407 3675 270
Size: 46 414 1498 432 747 268 998
Fragments arranged by size:
1498 998 747 432 414 268 46
EcoO109I rG'GnC_Cy
Cuts at: 899 1542 899
Size: 643 3760
EcoRI G'AATT_C
Cuts at: 1407 1407
Size: 4403
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4403
FspI TGC'GCA
Cuts at: 2184 2184
Size: 4403
HaeII r_GCGC'y
Cuts at: 482 1837 2085 3107 4086 482
Size: 1355 248 1022 979 799
Fragments arranged by size:
1355 1022 979 799 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 925 937 952 957 969 984 2245 2275 2277
Size: 12 15 5 12 15 1261 30 2
Cuts at: 2277 2307 925
Size: 30 3021
Fragments arranged by size:
3021 1261 30 30 15 15 12 12
5 2
HincII GTy'rAC
Cuts at: 1442 1442
Size: 4403
HindIII A'AGCT_T
Cuts at: 1305 1305
Size: 4403
KasI G'GCGC_C
Cuts at: 1833 2081 1833
Size: 248 4155
KpnI G_GTAC'C
Cuts at: 1423 1423
Size: 4403
MluI A'CGCG_T
Cuts at: 3836 3836
Size: 4403
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3074 3258 3074
Size: 184 4219
MscI TGG'CCA
Cuts at: 2164 2164
Size: 4403
MslI CAynn'nnrTG
Cuts at: 1051 2392 2519 1051
Size: 1341 127 2935
Fragments arranged by size:
2935 1341 127
MspA1I CmG'CkG
Cuts at: 1514 1828 2188 3201 3446 1514
Size: 314 360 1013 245 2471
Fragments arranged by size:
2471 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2584 2584
Size: 4403
NarI GG'CG_CC
Cuts at: 1834 2082 1834
Size: 248 4155
NcoI C'CATG_G
Cuts at: 107 107
Size: 4403
NgoMIV G'CCGG_C
Cuts at: 2582 2582
Size: 4403
NspI r_CATG'y
Cuts at: 695 1456 1956 2863 695
Size: 761 500 907 2235
Fragments arranged by size:
2235 907 761 500
PciI A'CATG_T
Cuts at: 691 2859 691
Size: 2168 2235
PfoI T'CCnGG_A
Cuts at: 152 152
Size: 4403
PmeI GTTT'AAAC
Cuts at: 1402 1402
Size: 4403
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3542 3574 3542
Size: 32 4371
PsiI TTA'TAA
Cuts at: 995 995
Size: 4403
PspOMI G'GGCC_C
Cuts at: 899 899
Size: 4403
PstI C_TGCA'G
Cuts at: 1290 1450 2135 1290
Size: 160 685 3558
Fragments arranged by size:
3558 685 160
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4403
PvuII CAG'CTG
Cuts at: 1828 2188 1828
Size: 360 4043
RsrII CG'GwC_CG
Cuts at: 2598 2598
Size: 4403
SacI G_AGCT'C
Cuts at: 1417 1417
Size: 4403
SalI G'TCGA_C
Cuts at: 1440 1440
Size: 4403
SapI GCTCTTCn'nnn_
Cuts at: 2426 2636 2426
Size: 210 4193
SbfI CC_TGCA'GG
Cuts at: 1450 1450
Size: 4403
ScaI AGT'ACT
Cuts at: 3920 3920
Size: 4403
SexAI A'CCwGG_T
Cuts at: 549 549
Size: 4403
SfcI C'TryA_G
Cuts at: 33 204 1286 1446 2131 3124 3315 33
Size: 171 1082 160 685 993 191 1121
Fragments arranged by size:
1121 1082 993 685 191 171 160
SfoI GGC'GCC
Cuts at: 1835 2083 1835
Size: 248 4155
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4403
SmaI CCC'GGG
Cuts at: 1425 1425
Size: 4403
SmlI C'TyrA_G
Cuts at: 1558 2473 2965 3227 1558
Size: 915 492 262 2734
Fragments arranged by size:
2734 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4403
SphI G_CATG'C
Cuts at: 1456 1956 1456
Size: 500 3903
SspI AAT'ATT
Cuts at: 837 837
Size: 4403
StyI C'CwwG_G
Cuts at: 107 113 1537 1891 107
Size: 6 1424 354 2619
Fragments arranged by size:
2619 1424 354 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1986 2119 2578 2787 1986
Size: 133 459 209 3602
Fragments arranged by size:
3602 459 209 133
TatI w'GTAC_w
Cuts at: 376 3918 376
Size: 3542 861
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3920 3952 3920
Size: 32 4371
Tth111I GACn'n_nGTC
Cuts at: 2200 2200
Size: 4403
XbaI T'CTAG_A
Cuts at: 955 1434 955
Size: 479 3924
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 256 910 1898 256
Size: 654 988 2761
Fragments arranged by size:
2761 988 654
XmaI C'CCGG_G
Cuts at: 1423 1423
Size: 4403
XmnI GAAnn'nnTTC
Cuts at: 509 509
Size: 4403
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI