Restriction map for
pF1KE0750
(Circular) (Six-base) MAPSORT of: pF1KE0750.seq Check: 1912 from: 1 to: 4391
pF1KE0750 4391 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AccI GT'mk_AC
Cuts at: 1429 1429
Size: 4391
Acc65I G'GTAC_C
Cuts at: 1407 1407
Size: 4391
AclI AA'CG_TT
Cuts at: 4231 4231
Size: 4391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 394 492 2216 2648 3395 3663 353
Size: 41 98 1724 432 747 268 1081
Fragments arranged by size:
1724 1081 747 432 268 98 41
AflII C'TTAA_G
Cuts at: 526 526
Size: 4391
AflIII A'CryG_T
Cuts at: 1107 1743 2847 3824 1107
Size: 636 1104 977 1674
Fragments arranged by size:
1674 1104 977 636
AgeI A'CCGG_T
Cuts at: 203 2779 203
Size: 2576 1815
AhdI GACnn_n'nnGTC
Cuts at: 204 204
Size: 4391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 616 616
Size: 4391
AlwNI CAG_nnn'CTG
Cuts at: 1696 3263 1696
Size: 1567 2824
ApaLI G'TGCA_C
Cuts at: 601 3161 601
Size: 2560 1831
ApoI r'AATT_y
Cuts at: 210 637 695 805 1002 1031 1283 1395 3744
Size: 427 58 110 197 29 252 112 2349
Cuts at: 3744 4000 210
Size: 256 601
Fragments arranged by size:
2349 601 427 256 252 197 112 110
58 29
AseI AT'TA_AT
Cuts at: 20 20
Size: 4391
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
AvaI C'yCGr_G
Cuts at: 1411 1411
Size: 4391
BamHI G'GATC_C
Cuts at: 1416 1416
Size: 4391
BanI G'GyrC_C
Cuts at: 1407 1821 2069 2104 1407
Size: 414 248 35 3694
Fragments arranged by size:
3694 414 248 35
BanII G_rGCy'C
Cuts at: 546 1405 2435 546
Size: 859 1030 2502
Fragments arranged by size:
2502 1030 859
BbeI G_GCGC'C
Cuts at: 1825 2073 1825
Size: 248 4143
BbsI GAAGACnn'nnnn_
Cuts at: 498 651 714 1333 498
Size: 153 63 619 3556
Fragments arranged by size:
3556 619 153 63
BciVI GTATCCnnnnn_n'
Cuts at: 440 2289 3050 4391 440
Size: 1849 761 1341 440
Fragments arranged by size:
1849 1341 761 440
BclI T'GATC_A
Cuts at: 1911 1911
Size: 4391
BglII A'GATC_T
Cuts at: 1255 1906 3943 1255
Size: 651 2037 1703
Fragments arranged by size:
2037 1703 651
BlpI GC'TnA_GC
Cuts at: 1503 1503
Size: 4391
Bme1580I G_kGCm'C
Cuts at: 605 2016 2109 3165 605
Size: 1411 93 1056 1831
Fragments arranged by size:
1831 1411 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 452 1603 1684 2019 4163 452
Size: 1151 81 335 2144 680
Fragments arranged by size:
2144 1151 680 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 814 2758 814
Size: 1944 2447
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 656 1229 340
Size: 316 573 3502
Fragments arranged by size:
3502 573 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1567 2446 2938 3236 1567
Size: 879 492 298 2722
Fragments arranged by size:
2722 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4095 4095
Size: 4391
BsaAI yAC'GTr
Cuts at: 1744 1744
Size: 4391
BsaBI GATnn'nnATC
Cuts at: 6 1905 3942 6
Size: 1899 2037 455
Fragments arranged by size:
2037 1899 455
BsaHI Gr'CG_yC
Cuts at: 1822 2070 2772 4268 1822
Size: 248 702 1496 1945
Fragments arranged by size:
1945 1496 702 248
BsaWI w'CCGG_w
Cuts at: 203 1580 2101 2779 3053 3200 3611 203
Size: 1377 521 678 274 147 411 983
Fragments arranged by size:
1377 983 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 654 684 1370 1400 654
Size: 30 686 30 3645
Fragments arranged by size:
3645 686 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 280
Size: 4391
BseYI C'CCAG_C
Cuts at: 1816 2574 3151 3647 1816
Size: 758 577 496 2560
Fragments arranged by size:
2560 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 920 920
Size: 4391
BsiEI CG_ry'CG
Cuts at: 104 1933 1979 3187 104
Size: 1829 46 1208 1308
Fragments arranged by size:
1829 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 546 605 1405 2183 2373 3165 546
Size: 59 800 778 190 792 1772
Fragments arranged by size:
1772 800 792 778 190 59
Bsp1286I G_dGCh'C
Cuts at: 546 605 1405 2016 2109 2183 2373 2435 3165
Size: 59 800 611 93 74 190 62 730
Cuts at: 3165 546
Size: 1772
Fragments arranged by size:
1772 800 730 611 190 93 74 62
59
BspEI T'CCGG_A
Cuts at: 3611 3611
Size: 4391
BspHI T'CATG_A
Cuts at: 3567 3567
Size: 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1441 1655 1957 2338 1441
Size: 214 302 381 3494
Fragments arranged by size:
3494 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2683 2737 4221 4386 50
Size: 2633 54 1484 165 55
Fragments arranged by size:
2633 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2303 2303
Size: 4391
BsrFI r'CCGG_y
Cuts at: 203 2389 2570 2779 203
Size: 2186 181 209 1815
Fragments arranged by size:
2186 1815 209 181
BssSI C'ACGA_G
Cuts at: 2662 3020 2662
Size: 358 4033
BstAPI GCAn_nnn'nTGC
Cuts at: 1768 1768
Size: 4391
BstBI TT'CG_AA
Cuts at: 2752 2752
Size: 4391
BstXI CCAn_nnnn'nTGG
Cuts at: 269 269
Size: 4391
BstYI r'GATC_y
Cuts at: 1 991 1255 1416 1883 1906 2241 2487 3499
Size: 990 264 161 467 23 335 246 1012
Cuts at: 3499 3585 3597 3943 1
Size: 86 12 346 449
Fragments arranged by size:
1012 990 467 449 346 335 264 246
161 86 23 12
BtgI C'CryG_G
Cuts at: 129 129
Size: 4391
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2341 2522 2685 2341
Size: 181 163 4047
Fragments arranged by size:
4047 181 163
BtsI GCAGTG_nn'
Cuts at: 859 1634 859
Size: 775 3616
DraI TTT'AAA
Cuts at: 1390 1390
Size: 4391
DrdI GACnn_nn'nnGTC
Cuts at: 2097 2955 2097
Size: 858 3533
EaeI y'GGCC_r
Cuts at: 1155 1976 2150 2541 2568 3913 1155
Size: 821 174 391 27 1345 1633
Fragments arranged by size:
1633 1345 821 391 174 27
EagI C'GGCC_G
Cuts at: 1976 1976
Size: 4391
EarI CTCTTCn'nnn_
Cuts at: 2414 2624 2414
Size: 210 4181
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2599 2909 3055 4201 2599
Size: 310 146 1146 2789
Fragments arranged by size:
2789 1146 310 146
EcoICRI GAG'CTC
Cuts at: 544 1403 544
Size: 859 3532
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 353 394 492 656 1229 2216 2648 3395
Size: 13 41 98 164 573 987 432 747
Cuts at: 3395 3663 340
Size: 268 1068
Fragments arranged by size:
1068 987 747 573 432 268 164 98
41 13
EcoO109I rG'GnC_Cy
Cuts at: 1530 1530
Size: 4391
EcoRI G'AATT_C
Cuts at: 1283 1395 1283
Size: 112 4279
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 4391
FspI TGC'GCA
Cuts at: 2172 2172
Size: 4391
HaeII r_GCGC'y
Cuts at: 1825 2073 3095 4074 1825
Size: 248 1022 979 2142
Fragments arranged by size:
2142 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 190 222 467 499 2233 2263 2265 2295 190
Size: 32 245 32 1734 30 2 30 2286
Fragments arranged by size:
2286 1734 245 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1430 1430
Size: 4391
KasI G'GCGC_C
Cuts at: 1821 2069 1821
Size: 248 4143
KpnI G_GTAC'C
Cuts at: 1411 1411
Size: 4391
MluI A'CGCG_T
Cuts at: 3824 3824
Size: 4391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3062 3246 3062
Size: 184 4207
MscI TGG'CCA
Cuts at: 1157 2152 1157
Size: 995 3396
MslI CAynn'nnrTG
Cuts at: 598 1056 2380 2507 598
Size: 458 1324 127 2482
Fragments arranged by size:
2482 1324 458 127
MspA1I CmG'CkG
Cuts at: 1502 1816 2176 3189 3434 1502
Size: 314 360 1013 245 2459
Fragments arranged by size:
2459 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2572 2572
Size: 4391
NarI GG'CG_CC
Cuts at: 1822 2070 1822
Size: 248 4143
NcoI C'CATG_G
Cuts at: 129 129
Size: 4391
NgoMIV G'CCGG_C
Cuts at: 2570 2570
Size: 4391
NspI r_CATG'y
Cuts at: 943 1111 1444 1944 2851 943
Size: 168 333 500 907 2483
Fragments arranged by size:
2483 907 500 333 168
PasI CC'CwG_GG
Cuts at: 143 143
Size: 4391
PciI A'CATG_T
Cuts at: 1107 2847 1107
Size: 1740 2651
PmeI GTTT'AAAC
Cuts at: 1390 1390
Size: 4391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 325 357 3530 3562 325
Size: 32 3173 32 1154
Fragments arranged by size:
3173 1154 32 32
PstI C_TGCA'G
Cuts at: 1438 2123 1438
Size: 685 3706
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4391
PvuII CAG'CTG
Cuts at: 1816 2176 1816
Size: 360 4031
RsrII CG'GwC_CG
Cuts at: 2586 2586
Size: 4391
SacI G_AGCT'C
Cuts at: 546 1405 546
Size: 859 3532
SalI G'TCGA_C
Cuts at: 1428 1428
Size: 4391
SapI GCTCTTCn'nnn_
Cuts at: 2414 2624 2414
Size: 210 4181
SbfI CC_TGCA'GG
Cuts at: 1438 1438
Size: 4391
ScaI AGT'ACT
Cuts at: 3908 3908
Size: 4391
SfcI C'TryA_G
Cuts at: 33 303 468 1434 2119 3112 3303 33
Size: 270 165 966 685 993 191 1121
Fragments arranged by size:
1121 993 966 685 270 191 165
SfoI GGC'GCC
Cuts at: 1823 2071 1823
Size: 248 4143
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
SmaI CCC'GGG
Cuts at: 1413 1413
Size: 4391
SmlI C'TyrA_G
Cuts at: 526 1546 2461 2953 3215 526
Size: 1020 915 492 262 1702
Fragments arranged by size:
1702 1020 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4391
SphI G_CATG'C
Cuts at: 1444 1944 1444
Size: 500 3891
SspI AAT'ATT
Cuts at: 437 437
Size: 4391
StuI AGG'CCT
Cuts at: 677 677
Size: 4391
StyI C'CwwG_G
Cuts at: 129 1525 1879 129
Size: 1396 354 2641
Fragments arranged by size:
2641 1396 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1974 2107 2566 2775 1974
Size: 133 459 209 3590
Fragments arranged by size:
3590 459 209 133
TatI w'GTAC_w
Cuts at: 1172 3906 1172
Size: 2734 1657
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 471 503 654 686 3908 3940 471
Size: 32 151 32 3222 32 922
Fragments arranged by size:
3222 922 151 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2188 2188
Size: 4391
XbaI T'CTAG_A
Cuts at: 1422 1422
Size: 4391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1886 1886
Size: 4391
XmaI C'CCGG_G
Cuts at: 1411 1411
Size: 4391
XmnI GAAnn'nnTTC
Cuts at: 910 1078 1246 1330 910
Size: 168 168 84 3971
Fragments arranged by size:
3971 168 168 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AleI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI