Restriction map for
pF1KE0745
(Circular) (Six-base) MAPSORT of: pF1KE0745.seq Check: 9125 from: 1 to: 4073
pF1KE0745 4073 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 673 1111 673
Size: 438 3635
Acc65I G'GTAC_C
Cuts at: 1089 1089
Size: 4073
AclI AA'CG_TT
Cuts at: 1067 3913 1067
Size: 2846 1227
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 1898 2330 3077 3345 345
Size: 1553 432 747 268 1073
Fragments arranged by size:
1553 1073 747 432 268
AflIII A'CryG_T
Cuts at: 856 1425 2529 3506 856
Size: 569 1104 977 1423
Fragments arranged by size:
1423 1104 977 569
AgeI A'CCGG_T
Cuts at: 2461 2461
Size: 4073
AlwNI CAG_nnn'CTG
Cuts at: 1378 2945 1378
Size: 1567 2506
ApaI G_GGCC'C
Cuts at: 638 638
Size: 4073
ApaLI G'TGCA_C
Cuts at: 2843 2843
Size: 4073
ApoI r'AATT_y
Cuts at: 1077 3426 3682 1077
Size: 2349 256 1468
Fragments arranged by size:
2349 1468 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4073
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
AvaI C'yCGr_G
Cuts at: 1093 1093
Size: 4073
BamHI G'GATC_C
Cuts at: 206 1098 206
Size: 892 3181
BanI G'GyrC_C
Cuts at: 1089 1503 1751 1786 1089
Size: 414 248 35 3376
Fragments arranged by size:
3376 414 248 35
BanII G_rGCy'C
Cuts at: 197 261 638 881 1087 2117 197
Size: 64 377 243 206 1030 2153
Fragments arranged by size:
2153 1030 377 243 206 64
BbeI G_GCGC'C
Cuts at: 1507 1755 1507
Size: 248 3825
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 967 1001 90
Size: 34 843 34 3162
Fragments arranged by size:
3162 843 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 618 1971 2732 4073 618
Size: 1353 761 1341 618
Fragments arranged by size:
1353 1341 761 618
BclI T'GATC_A
Cuts at: 1593 1593
Size: 4073
BglII A'GATC_T
Cuts at: 1588 3625 1588
Size: 2037 2036
BlpI GC'TnA_GC
Cuts at: 1185 1185
Size: 4073
Bme1580I G_kGCm'C
Cuts at: 638 1698 1791 2847 638
Size: 1060 93 1056 1864
Fragments arranged by size:
1864 1060 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 131 1285 1366 1701 3845 131
Size: 1154 81 335 2144 359
Fragments arranged by size:
2144 1154 359 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 4073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 137
Size: 4073
Bpu10I CC'TnA_GC
Cuts at: 920 920
Size: 4073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 538 1249 2128 2620 2918 184
Size: 354 711 879 492 298 1339
Fragments arranged by size:
1339 879 711 492 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 364 856 3777 364
Size: 492 2921 660
Fragments arranged by size:
2921 660 492
BsaAI yAC'GTr
Cuts at: 1426 1426
Size: 4073
BsaBI GATnn'nnATC
Cuts at: 6 1587 3624 6
Size: 1581 2037 455
Fragments arranged by size:
2037 1581 455
BsaHI Gr'CG_yC
Cuts at: 1504 1752 2454 3950 1504
Size: 248 702 1496 1627
Fragments arranged by size:
1627 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1262 1783 2461 2735 2882 3293 1262
Size: 521 678 274 147 411 2042
Fragments arranged by size:
2042 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 172 175 187 544 547 172
Size: 3 12 357 3 3698
Fragments arranged by size:
3698 357 12 3 3
BseYI C'CCAG_C
Cuts at: 630 663 1498 2256 2833 3329 630
Size: 33 835 758 577 496 1374
Fragments arranged by size:
1374 835 758 577 496 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 736
Size: 4073
BsiEI CG_ry'CG
Cuts at: 104 1615 1661 2869 104
Size: 1511 46 1208 1308
Fragments arranged by size:
1511 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 197 261 881 1087 1865 2055 2847 197
Size: 64 620 206 778 190 792 1423
Fragments arranged by size:
1423 792 778 620 206 190 64
Bsp1286I G_dGCh'C
Cuts at: 197 261 638 881 1087 1698 1791 1865 2055
Size: 64 377 243 206 611 93 74 190
Cuts at: 2055 2117 2847 197
Size: 62 730 1423
Fragments arranged by size:
1423 730 611 377 243 206 190 93
74 64 62
BspEI T'CCGG_A
Cuts at: 3293 3293
Size: 4073
BspHI T'CATG_A
Cuts at: 3249 3249
Size: 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 878 1123 1337 1639 2020 878
Size: 245 214 302 381 2931
Fragments arranged by size:
2931 381 302 245 214
BsrBI CCG'CTC
Cuts at: 50 2365 2419 3903 4068 50
Size: 2315 54 1484 165 55
Fragments arranged by size:
2315 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 995 1985 995
Size: 990 3083
BsrFI r'CCGG_y
Cuts at: 711 2071 2252 2461 711
Size: 1360 181 209 2323
Fragments arranged by size:
2323 1360 209 181
BssSI C'ACGA_G
Cuts at: 2344 2702 2344
Size: 358 3715
BstAPI GCAn_nnn'nTGC
Cuts at: 1450 1450
Size: 4073
BstBI TT'CG_AA
Cuts at: 130 2434 130
Size: 2304 1769
BstYI r'GATC_y
Cuts at: 1 206 1098 1565 1588 1923 2169 3181 3267
Size: 205 892 467 23 335 246 1012 86
Cuts at: 3267 3279 3625 1
Size: 12 346 449
Fragments arranged by size:
1012 892 467 449 346 335 246 205
86 23 12
BtgI C'CryG_G
Cuts at: 780 780
Size: 4073
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2023 2204 2367 2023
Size: 181 163 3729
Fragments arranged by size:
3729 181 163
BtsI GCAGTG_nn'
Cuts at: 262 1316 262
Size: 1054 3019
DraI TTT'AAA
Cuts at: 1072 1072
Size: 4073
DrdI GACnn_nn'nnGTC
Cuts at: 181 1779 2637 181
Size: 1598 858 1617
Fragments arranged by size:
1617 1598 858
EaeI y'GGCC_r
Cuts at: 215 458 653 936 1658 1832 2223 2250 3595
Size: 243 195 283 722 174 391 27 1345
Cuts at: 3595 215
Size: 693
Fragments arranged by size:
1345 722 693 391 283 243 195 174
27
EagI C'GGCC_G
Cuts at: 1658 1658
Size: 4073
EarI CTCTTCn'nnn_
Cuts at: 886 2096 2306 886
Size: 1210 210 2653
Fragments arranged by size:
2653 1210 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2281 2591 2737 3883 2281
Size: 310 146 1146 2471
Fragments arranged by size:
2471 1146 310 146
EcoICRI GAG'CTC
Cuts at: 195 259 879 1085 195
Size: 64 620 206 3183
Fragments arranged by size:
3183 620 206 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 345 1898 2330 3077 3345 137
Size: 208 1553 432 747 268 865
Fragments arranged by size:
1553 865 747 432 268 208
EcoNI CCTnn'n_nnAGG
Cuts at: 919 919
Size: 4073
EcoO109I rG'GnC_Cy
Cuts at: 513 537 634 1212 513
Size: 24 97 578 3374
Fragments arranged by size:
3374 578 97 24
EcoRI G'AATT_C
Cuts at: 1077 1077
Size: 4073
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 4073
FspI TGC'GCA
Cuts at: 1854 1854
Size: 4073
HaeII r_GCGC'y
Cuts at: 1507 1755 2777 3756 1507
Size: 248 1022 979 1824
Fragments arranged by size:
1824 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 469 501 1915 1945 1947 1977 469
Size: 32 1414 30 2 30 2565
Fragments arranged by size:
2565 1414 32 30 30 2
HincII GTy'rAC
Cuts at: 1112 1112
Size: 4073
KasI G'GCGC_C
Cuts at: 1503 1751 1503
Size: 248 3825
KpnI G_GTAC'C
Cuts at: 1093 1093
Size: 4073
MluI A'CGCG_T
Cuts at: 856 3506 856
Size: 2650 1423
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2744 2928 2744
Size: 184 3889
MscI TGG'CCA
Cuts at: 217 460 938 1834 217
Size: 243 478 896 2456
Fragments arranged by size:
2456 896 478 243
MslI CAynn'nnrTG
Cuts at: 2062 2189 2062
Size: 127 3946
MspA1I CmG'CkG
Cuts at: 611 663 782 1184 1498 1858 2871 3116 611
Size: 52 119 402 314 360 1013 245 1568
Fragments arranged by size:
1568 1013 402 360 314 245 119 52
NaeI GCC'GGC
Cuts at: 2254 2254
Size: 4073
NarI GG'CG_CC
Cuts at: 1504 1752 1504
Size: 248 3825
NgoMIV G'CCGG_C
Cuts at: 2252 2252
Size: 4073
NspI r_CATG'y
Cuts at: 1126 1626 2533 1126
Size: 500 907 2666
Fragments arranged by size:
2666 907 500
PciI A'CATG_T
Cuts at: 2529 2529
Size: 4073
PmeI GTTT'AAAC
Cuts at: 1072 1072
Size: 4073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3212 3244 3212
Size: 32 4041
PpuMI rG'GwC_Cy
Cuts at: 513 537 513
Size: 24 4049
PspOMI G'GGCC_C
Cuts at: 634 634
Size: 4073
PstI C_TGCA'G
Cuts at: 875 1120 1805 875
Size: 245 685 3143
Fragments arranged by size:
3143 685 245
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4073
PvuII CAG'CTG
Cuts at: 663 1498 1858 663
Size: 835 360 2878
Fragments arranged by size:
2878 835 360
RsrII CG'GwC_CG
Cuts at: 2268 2268
Size: 4073
SacI G_AGCT'C
Cuts at: 197 261 881 1087 197
Size: 64 620 206 3183
Fragments arranged by size:
3183 620 206 64
SacII CC_GC'GG
Cuts at: 783 783
Size: 4073
SalI G'TCGA_C
Cuts at: 1110 1110
Size: 4073
SapI GCTCTTCn'nnn_
Cuts at: 886 2096 2306 886
Size: 1210 210 2653
Fragments arranged by size:
2653 1210 210
SbfI CC_TGCA'GG
Cuts at: 875 1120 875
Size: 245 3828
ScaI AGT'ACT
Cuts at: 3590 3590
Size: 4073
SexAI A'CCwGG_T
Cuts at: 149 149
Size: 4073
SfcI C'TryA_G
Cuts at: 33 871 1116 1801 2794 2985 33
Size: 838 245 685 993 191 1121
Fragments arranged by size:
1121 993 838 685 245 191
SfoI GGC'GCC
Cuts at: 1505 1753 1505
Size: 248 3825
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
SmaI CCC'GGG
Cuts at: 1095 1095
Size: 4073
SmlI C'TyrA_G
Cuts at: 199 553 1228 2143 2635 2897 199
Size: 354 675 915 492 262 1375
Fragments arranged by size:
1375 915 675 492 354 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4073
SphI G_CATG'C
Cuts at: 1126 1626 1126
Size: 500 3573
StyI C'CwwG_G
Cuts at: 275 944 1207 1561 275
Size: 669 263 354 2787
Fragments arranged by size:
2787 669 354 263
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 880 1656 1789 2248 2457 880
Size: 776 133 459 209 2496
Fragments arranged by size:
2496 776 459 209 133
TatI w'GTAC_w
Cuts at: 314 3588 314
Size: 3274 799
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 440 472 3590 3622 440
Size: 32 3118 32 891
Fragments arranged by size:
3118 891 32 32
Tth111I GACn'n_nGTC
Cuts at: 481 1870 481
Size: 1389 2684
XbaI T'CTAG_A
Cuts at: 1104 1104
Size: 4073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1568 1568
Size: 4073
XmaI C'CCGG_G
Cuts at: 1093 1093
Size: 4073
XmnI GAAnn'nnTTC
Cuts at: 418 418
Size: 4073
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI