Restriction map for
pF1KE0742
(Circular) (Six-base) MAPSORT of: pF1KE0742.seq Check: 4271 from: 1 to: 4385
pF1KE0742 4385 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1423 1423
Size: 4385
Acc65I G'GTAC_C
Cuts at: 1401 1401
Size: 4385
AclI AA'CG_TT
Cuts at: 4225 4225
Size: 4385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 417 2210 2642 3389 3657 171
Size: 246 1793 432 747 268 899
Fragments arranged by size:
1793 899 747 432 268 246
AflIII A'CryG_T
Cuts at: 206 384 447 944 1282 1737 2841 3818 206
Size: 178 63 497 338 455 1104 977 773
Fragments arranged by size:
1104 977 773 497 455 338 178 63
AgeI A'CCGG_T
Cuts at: 2773 2773
Size: 4385
AleI CACnn'nnGTG
Cuts at: 229 697 229
Size: 468 3917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 306 494 306
Size: 188 4197
AlwNI CAG_nnn'CTG
Cuts at: 1155 1690 3257 1155
Size: 535 1567 2283
Fragments arranged by size:
2283 1567 535
ApaLI G'TGCA_C
Cuts at: 3155 3155
Size: 4385
ApoI r'AATT_y
Cuts at: 1389 3738 3994 1389
Size: 2349 256 1780
Fragments arranged by size:
2349 1780 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4385
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
AvaI C'yCGr_G
Cuts at: 136 1405 136
Size: 1269 3116
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 996 1029 996
Size: 33 4352
BamHI G'GATC_C
Cuts at: 1410 1410
Size: 4385
BanI G'GyrC_C
Cuts at: 985 1160 1401 1815 2063 2098 985
Size: 175 241 414 248 35 3272
Fragments arranged by size:
3272 414 248 241 175 35
BanII G_rGCy'C
Cuts at: 185 647 673 1067 1134 1349 1399 2429 185
Size: 462 26 394 67 215 50 1030 2141
Fragments arranged by size:
2141 1030 462 394 215 67 50 26
BbeI G_GCGC'C
Cuts at: 1819 2067 1819
Size: 248 4137
BbsI GAAGACnn'nnnn_
Cuts at: 877 877
Size: 4385
BciVI GTATCCnnnnn_n'
Cuts at: 2283 3044 4385 2283
Size: 761 1341 2283
Fragments arranged by size:
2283 1341 761
BclI T'GATC_A
Cuts at: 1905 1905
Size: 4385
BglII A'GATC_T
Cuts at: 1900 3937 1900
Size: 2037 2348
BlpI GC'TnA_GC
Cuts at: 1497 1497
Size: 4385
Bme1580I G_kGCm'C
Cuts at: 799 2010 2103 3159 799
Size: 1211 93 1056 2025
Fragments arranged by size:
2025 1211 1056 93
BmgBI CAC'GTC
Cuts at: 207 207
Size: 4385
BmrI ACTGGGnnnn_n'
Cuts at: 751 1269 1597 1678 2013 4157 751
Size: 518 328 81 335 2144 979
Fragments arranged by size:
2144 979 518 335 328 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 345 2752 345
Size: 2407 1978
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1148 1148
Size: 4385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1561 2440 2932 3230 1561
Size: 879 492 298 2716
Fragments arranged by size:
2716 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 490 752 4089 490
Size: 262 3337 786
Fragments arranged by size:
3337 786 262
BsaAI yAC'GTr
Cuts at: 385 448 1738 385
Size: 63 1290 3032
Fragments arranged by size:
3032 1290 63
BsaBI GATnn'nnATC
Cuts at: 6 814 1899 3936 6
Size: 808 1085 2037 455
Fragments arranged by size:
2037 1085 808 455
BsaHI Gr'CG_yC
Cuts at: 689 722 959 1816 2064 2766 4262 689
Size: 33 237 857 248 702 1496 812
Fragments arranged by size:
1496 857 812 702 248 237 33
BsaWI w'CCGG_w
Cuts at: 725 1574 2095 2773 3047 3194 3605 725
Size: 849 521 678 274 147 411 1505
Fragments arranged by size:
1505 849 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 309 339 595 625 309
Size: 30 256 30 4069
Fragments arranged by size:
4069 256 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1236 1236
Size: 4385
BseYI C'CCAG_C
Cuts at: 264 954 1810 2568 3145 3641 264
Size: 690 856 758 577 496 1008
Fragments arranged by size:
1008 856 758 690 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1127 1192 1127
Size: 65 4320
BsiEI CG_ry'CG
Cuts at: 104 1927 1973 3181 104
Size: 1823 46 1208 1308
Fragments arranged by size:
1823 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 458 647 843 1264 1349 1399 2177 2367 3159
Size: 189 196 421 85 50 778 190 792
Cuts at: 3159 458
Size: 1684
Fragments arranged by size:
1684 792 778 421 196 190 189 85
50
Bsp1286I G_dGCh'C
Cuts at: 185 458 647 673 799 843 1067 1134 1264
Size: 273 189 26 126 44 224 67 130
Cuts at: 1264 1349 1399 2010 2103 2177 2367 2429 3159
Size: 85 50 611 93 74 190 62 730
Cuts at: 3159 185
Size: 1411
Fragments arranged by size:
1411 730 611 273 224 190 189 130
126 93 85 74 67 62 50 44
26
BspEI T'CCGG_A
Cuts at: 3605 3605
Size: 4385
BspHI T'CATG_A
Cuts at: 3561 3561
Size: 4385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1214 1361 1435 1649 1951 2332 1214
Size: 147 74 214 302 381 3267
Fragments arranged by size:
3267 381 302 214 147 74
BsrBI CCG'CTC
Cuts at: 50 606 1214 1295 2677 2731 4215 4380 50
Size: 556 608 81 1382 54 1484 165 55
Fragments arranged by size:
1484 1382 608 556 165 81 55 54
BsrDI GCAATG_nn'
Cuts at: 659 896 962 2297 659
Size: 237 66 1335 2747
Fragments arranged by size:
2747 1335 237 66
BsrFI r'CCGG_y
Cuts at: 1025 2383 2564 2773 1025
Size: 1358 181 209 2637
Fragments arranged by size:
2637 1358 209 181
BsrGI T'GTAC_A
Cuts at: 209 611 209
Size: 402 3983
BssSI C'ACGA_G
Cuts at: 2656 3014 2656
Size: 358 4027
BstAPI GCAn_nnn'nTGC
Cuts at: 711 1762 711
Size: 1051 3334
BstBI TT'CG_AA
Cuts at: 2746 2746
Size: 4385
BstXI CCAn_nnnn'nTGG
Cuts at: 939 939
Size: 4385
BstYI r'GATC_y
Cuts at: 1 162 1031 1410 1877 1900 2235 2481 3493
Size: 161 869 379 467 23 335 246 1012
Cuts at: 3493 3579 3591 3937 1
Size: 86 12 346 449
Fragments arranged by size:
1012 869 467 449 379 346 335 246
161 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1036 1156 1036
Size: 120 4265
BtgI C'CryG_G
Cuts at: 107 1165 107
Size: 1058 3327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2335 2516 2679 2335
Size: 181 163 4041
Fragments arranged by size:
4041 181 163
BtsI GCAGTG_nn'
Cuts at: 1144 1628 1144
Size: 484 3901
DraI TTT'AAA
Cuts at: 1384 1384
Size: 4385
DraIII CAC_nnn'GTG
Cuts at: 1321 1321
Size: 4385
DrdI GACnn_nn'nnGTC
Cuts at: 2091 2949 2091
Size: 858 3527
EaeI y'GGCC_r
Cuts at: 312 420 629 833 1088 1970 2144 2535 2562
Size: 108 209 204 255 882 174 391 27
Cuts at: 2562 3907 312
Size: 1345 790
Fragments arranged by size:
1345 882 790 391 255 209 204 174
108 27
EagI C'GGCC_G
Cuts at: 1970 1970
Size: 4385
EarI CTCTTCn'nnn_
Cuts at: 1194 2408 2618 1194
Size: 1214 210 2961
Fragments arranged by size:
2961 1214 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2593 2903 3049 4195 2593
Size: 310 146 1146 2783
Fragments arranged by size:
2783 1146 310 146
EcoICRI GAG'CTC
Cuts at: 645 1347 1397 645
Size: 702 50 3633
Fragments arranged by size:
3633 702 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 417 1148 2210 2642 3389 3657 171
Size: 246 731 1062 432 747 268 899
Fragments arranged by size:
1062 899 747 731 432 268 246
EcoNI CCTnn'n_nnAGG
Cuts at: 1090 1090
Size: 4385
EcoO109I rG'GnC_Cy
Cuts at: 129 411 1152 1524 129
Size: 282 741 372 2990
Fragments arranged by size:
2990 741 372 282
EcoRI G'AATT_C
Cuts at: 1389 1389
Size: 4385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 690 2404 690
Size: 1714 2671
FspI TGC'GCA
Cuts at: 2166 2166
Size: 4385
HaeII r_GCGC'y
Cuts at: 955 1819 2067 3089 4068 955
Size: 864 248 1022 979 1272
Fragments arranged by size:
1272 1022 979 864 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2227 2257 2259 2289 2227
Size: 30 2 30 4323
Fragments arranged by size:
4323 30 30 2
HincII GTy'rAC
Cuts at: 1424 1424
Size: 4385
KasI G'GCGC_C
Cuts at: 1815 2063 1815
Size: 248 4137
KpnI G_GTAC'C
Cuts at: 1405 1405
Size: 4385
MluI A'CGCG_T
Cuts at: 1282 3818 1282
Size: 2536 1849
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 512 1106 3056 3240 512
Size: 594 1950 184 1657
Fragments arranged by size:
1950 1657 594 184
MscI TGG'CCA
Cuts at: 314 631 835 1090 2146 314
Size: 317 204 255 1056 2553
Fragments arranged by size:
2553 1056 317 255 204
MslI CAynn'nnrTG
Cuts at: 229 487 619 697 2374 2501 229
Size: 258 132 78 1677 127 2113
Fragments arranged by size:
2113 1677 258 132 127 78
MspA1I CmG'CkG
Cuts at: 159 579 1496 1810 2170 3183 3428 159
Size: 420 917 314 360 1013 245 1116
Fragments arranged by size:
1116 1013 917 420 360 314 245
NaeI GCC'GGC
Cuts at: 2566 2566
Size: 4385
NarI GG'CG_CC
Cuts at: 1816 2064 1816
Size: 248 4137
NcoI C'CATG_G
Cuts at: 107 107
Size: 4385
NgoMIV G'CCGG_C
Cuts at: 2564 2564
Size: 4385
NspI r_CATG'y
Cuts at: 739 948 1438 1938 2845 739
Size: 209 490 500 907 2279
Fragments arranged by size:
2279 907 500 490 209
PciI A'CATG_T
Cuts at: 944 2841 944
Size: 1897 2488
PflMI CCAn_nnn'nTGG
Cuts at: 582 582
Size: 4385
PmeI GTTT'AAAC
Cuts at: 1384 1384
Size: 4385
PmlI CAC'GTG
Cuts at: 385 385
Size: 4385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 4353
PpuMI rG'GwC_Cy
Cuts at: 129 129
Size: 4385
PstI C_TGCA'G
Cuts at: 707 1151 1432 2117 707
Size: 444 281 685 2975
Fragments arranged by size:
2975 685 444 281
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4385
PvuII CAG'CTG
Cuts at: 159 579 1810 2170 159
Size: 420 1231 360 2374
Fragments arranged by size:
2374 1231 420 360
RsrII CG'GwC_CG
Cuts at: 2580 2580
Size: 4385
SacI G_AGCT'C
Cuts at: 647 1349 1399 647
Size: 702 50 3633
Fragments arranged by size:
3633 702 50
SalI G'TCGA_C
Cuts at: 1422 1422
Size: 4385
SapI GCTCTTCn'nnn_
Cuts at: 2408 2618 2408
Size: 210 4175
SbfI CC_TGCA'GG
Cuts at: 1432 1432
Size: 4385
ScaI AGT'ACT
Cuts at: 3902 3902
Size: 4385
SfcI C'TryA_G
Cuts at: 33 703 1147 1428 2113 3106 3297 33
Size: 670 444 281 685 993 191 1121
Fragments arranged by size:
1121 993 685 670 444 281 191
SfoI GGC'GCC
Cuts at: 1817 2065 1817
Size: 248 4137
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
SmaI CCC'GGG
Cuts at: 1407 1407
Size: 4385
SmlI C'TyrA_G
Cuts at: 1540 2455 2947 3209 1540
Size: 915 492 262 2716
Fragments arranged by size:
2716 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4385
SphI G_CATG'C
Cuts at: 739 1438 1938 739
Size: 699 500 3186
Fragments arranged by size:
3186 699 500
StyI C'CwwG_G
Cuts at: 107 146 665 1091 1519 1873 107
Size: 39 519 426 428 354 2619
Fragments arranged by size:
2619 519 428 426 354 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 159 1158 1968 2101 2560 2769 159
Size: 999 810 133 459 209 1775
Fragments arranged by size:
1775 999 810 459 209 133
TatI w'GTAC_w
Cuts at: 209 362 611 893 1046 3900 209
Size: 153 249 282 153 2854 694
Fragments arranged by size:
2854 694 282 249 153 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 309 341 3902 3934 309
Size: 32 3561 32 760
Fragments arranged by size:
3561 760 32 32
Tth111I GACn'n_nGTC
Cuts at: 2182 2182
Size: 4385
XbaI T'CTAG_A
Cuts at: 1416 1416
Size: 4385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1880 1880
Size: 4385
XmaI C'CCGG_G
Cuts at: 1405 1405
Size: 4385
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI
BsmI BsmBI BssHII BstEII BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI